DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Pak and SKM1

DIOPT Version :9

Sequence 1:	NP_001138013.2	Gene:	Pak / 44039	FlyBaseID:	FBgn0267698	Length:	840	Species:	Drosophila melanogaster
Sequence 2:	NP_014528.1	Gene:	SKM1 / 854036	SGDID:	S000005473	Length:	655	Species:	Saccharomyces cerevisiae

Alignment Length:	776	Identity:	222/776 - (28%)
Similarity:	322/776 - (41%)	Gaps:	294/776 - (37%)

- Green bases have known domain annotations that are detailed below.

  Fly    83 ISYPTNFEHTVHVGFDAVTGEFTGMPEAWARLLMNSNISKQEQKKNPQAVLDVLKWFDNTTKQRP 147
            :|.||||.|.||||||...|.|.|:|::||:||..|.|:..:..:|.:||:..|:::::
Yeast   123 VSSPTNFTHKVHVGFDPKVGNFVGVPDSWAKLLQTSEITYDDWNRNSKAVIKALQFYED------ 181

  Fly   148 SSKYMTNAITT-----HSGSSLSRVSSSSPSSTPTDSELHGSNSGGNLIGVQLGSMTLGPNANNV 207
                 .|.:.|     |..:||    ...|..:||
Yeast   182 -----YNGLDTMQFNDHLNTSL----DLKPLKSPT------------------------------ 207

  Fly   208 AVAGQILGNHYQQQQQHLLQQQQPLLHQNHNQHHMGISQSHSYNFVGHTVSSSTSQHSSANEDDM 272
                           ::::.::.                        :::..|.|:.....:.|.
Yeast   208 ---------------RYIINKRT------------------------NSIKRSVSRTLRKGKTDS 233

  Fly   273 LGPQHPQQQPPPPPVASRPERTKSIYTRPIEDLQPAIIPMPVAPATTPATPLQNHRTPGGISAPA 337
            :.|.:..:..|.|    ||......:|. |||                    ...|..|.:
Yeast   234 ILPVYQSELKPFP----RPSDDDYKFTN-IED--------------------NKVREEGRV---- 269

  Fly   338 ASPMMHNNATTTLDKNKNNATPTSPTTTKTTAETEGTDATSTLLSATNPSCSTTPTTDP---APA 399
                 |.:..:|.|           :.||...:.|.....|.|....|.|     |..|   ||:
Yeast   270 -----HVSKESTAD-----------SQTKQLGKKEQKVIQSHLRRHDNNS-----TFRPHRLAPS 313

  Fly   400 APRGSLVETATAHATTAAATVATSTTTNHHSSASSFSSFPSFHDDDGTHHALPTIHCPTPTSSSR 464
            ||                      .|.||.|..       .:|.:|
Yeast   314 AP----------------------ATKNHDSKT-------KWHKED------------------- 330

  Fly   465 NSTVSFPVAVPLPPIVTPASPTPASIESPDLYTPEPTVAQVSAGGPSSQVAGNQIAVPQAAVAPA 529

Yeast   331 ----------------------------------------------------------------- 330

  Fly   530 ATPNTRAANAKKKKMSDE--EILEKLRTIVSVGDPNRKYTKMEKIGQGASGTVYTA--------- 583
                     ..:.|.:|:  ||:.|::|:....:|...:..:||.||||||.||.:
Yeast   331 ---------LLELKNNDDSNEIIMKMKTVAIDVNPRPYFQLVEKAGQGASGAVYLSKRIKLPQEN 386

  Fly   584 ----IESS----TGMEVAIKQMNLSQQPKKELIINEILVMRENKHPNVVNYLDSYLV-SEELWVV 639
                ::|.    .|..|||||:.||:||||:||:||:|||.:::..|:||:|::|:: .|||||:
Yeast   387 DPRFLKSHCHRVVGERVAIKQIRLSEQPKKQLIMNELLVMNDSRQENIVNFLEAYIIDDEELWVI 451

  Fly   640 MEYLPGGSLTDVVTETC----------MDEGQIAAVCREVLQALEFLHANQVIHRDIKSDNILLG 694
            |||:.||.|||::....          ::|.|:|.:.:|..|.|:|||..::||||||||||||.
Yeast   452 MEYMEGGCLTDILDAVARSNTGEHSSPLNENQMAYIVKETCQGLKFLHNKKIIHRDIKSDNILLN 516

  Fly   695 LDGSVKLTDFGFCAQISPEQSKRTTMVGTPYWMAPEVVTRKQYGPKVDLWSLGIMAIEMVEGEPP 759
            ..|.||:||||||.:::.::|||.||||||||||||:|.:|.|..|||:||||||.|||:|||||
Yeast   517 SQGLVKITDFGFCVELTEKRSKRATMVGTPYWMAPEIVNQKGYDEKVDVWSLGIMLIEMIEGEPP 581

  Fly   760 YLNENPLKALYLIATNGKPEIKEKDKLSSAFQDFLDQCLEVEVDRRASALDLLKHPFLKLA 820
            ||||:|||||||||.||.|:::..:.:|...:.|||.||:|.|:.|||...||...||.:|
Yeast   582 YLNEDPLKALYLIANNGSPKLRHPESVSKQTKQFLDACLQVNVESRASVRKLLTFEFLSMA 642

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Pak	NP_001138013.2	PBD	83..137	CDD:279166	25/53 (47%)
		STKc_PAK_I	558..818	CDD:270814	144/287 (50%)
		S_TKc	566..817	CDD:214567	142/278 (51%)
SKM1	NP_014528.1	PH_Cla4_Ste20	4..113	CDD:270097
		PBD	122..180	CDD:395634	26/56 (46%)
		STKc_PAK	359..640	CDD:270789	143/280 (51%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	1	0.950	-	0	Normalized mean entropy	S857
OMA	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000763
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100484
Panther	1	1.100	-	-	O	PTHR48015
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1405
SonicParanoid	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	7	6.890