DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Pak and shk2

DIOPT Version :9

Sequence 1:	NP_001138013.2	Gene:	Pak / 44039	FlyBaseID:	FBgn0267698	Length:	840	Species:	Drosophila melanogaster
Sequence 2:	NP_592864.1	Gene:	shk2 / 2541468	PomBaseID:	SPAC1F5.09c	Length:	589	Species:	Schizosaccharomyces pombe

Alignment Length:	765	Identity:	211/765 - (27%)
Similarity:	295/765 - (38%)	Gaps:	311/765 - (40%)

- Green bases have known domain annotations that are detailed below.

  Fly    83 ISYPTNFEHTVHVGFDAVTGEFTGMPEAWARLLMNSNISKQEQKKNPQAVLDVLKWFDNTTKQRP 147
            :|.|.|.:|.||||.|. .|.:.|:|:.|..||.:|:|:|||..:.|:||:..|.::   :||
pombe   129 VSSPMNPKHQVHVGIDN-EGNYVGLPKEWILLLQSSSITKQECMEEPKAVIQALDFY---SKQ-- 187

  Fly   148 SSKYMTNAITTHSGSSLSRVSSSSPSSTPTDSELHGSNSGGNLIGVQLGSMTLGPNANNVAVAGQ 212
                                       ..|.||
pombe   188 ---------------------------LDTTSE-------------------------------- 193

  Fly   213 ILGNHYQQQQQHLLQQQQPLLHQNHNQHHMGISQSHSYNFVGHTV-SSSTSQHSSANEDDMLGPQ 276
                                             ...|::|...|: .|||:.:|..:.|
pombe   194 ---------------------------------TKDSFSFCKETLPRSSTTSYSIRDAD------ 219

  Fly   277 HPQQQPPPPPVASRPERTKSIYTRPIEDLQPAIIPMPVAPATTPATPLQNHRTPGGISAPAASPM 341

pombe   220 ----------------------------------------------------------------- 219

  Fly   342 MHNNATTTLDKNKNNATPTSPTTTKTTAETEGTDATSTLLSATNPSCSTTPTTDPAPAAPRGSLV 406
            .|:..||:.....|......|.||                                        :
pombe   220 KHHKLTTSGVTKMNITERCKPKTT----------------------------------------I 244

  Fly   407 ETATAHATTAAATVATSTTTNHHSSASSFSSFPSFHDDDGTHHALPTIHCPTPTSSSRNSTVSFP 471
            :|...|
pombe   245 QTDKKH----------------------------------------------------------- 250

  Fly   472 VAVPLPPIVTPASPTPASIESPDLYTPEPTVAQVSAGGPSSQVAGNQIAVPQAAVAPAATPNTRA 536
                   |:.|.....:.:||  :.|.:.|...|.|.                           :
pombe   251 -------IIRPFIEDKSHVES--IMTGKVTKVPVKAD---------------------------S 279

  Fly   537 ANAKKKKMSDEEILEKLRTIVSVGDPNRKYTKMEKIGQGASGTVYTAI---ESSTGM--EVAIKQ 596
            .|...::|:|.:.|..|:..|:..||...:....|:||||||:||.|.   ....|:  .||||.
pombe   280 KNTLSRRMTDRQALAMLKDSVTSHDPVEYFNVKHKLGQGASGSVYLAKVVGGKQLGIFDSVAIKS 344

  Fly   597 MNLSQQPKKELIINEILVMRENKHPNVVNYLDSYLVSE-ELWVVMEYLPGGSLTDVVTETCMDEG 660
            ::|..|.:||||:|||.||||:.|||:|.||||:||.| .|||||||:..|||||::.::.:.|.
pombe   345 IDLQCQTRKELILNEITVMRESIHPNIVTYLDSFLVRERHLWVVMEYMNAGSLTDIIEKSKLTEA 409

  Fly   661 QIAAVCREVLQALEFLHANQVIHRDIKSDNILLGLDGSVKLTDFGFCAQISPEQSKRTTMVGTPY 725
            |||.:|.|..:.::.|||..:|||||||||:||...|::|:|||||||::|...:||.|||||||
pombe   410 QIARICLETCKGIQHLHARNIIHRDIKSDNVLLDNSGNIKITDFGFCARLSNRTNKRVTMVGTPY 474

  Fly   726 WMAPEVVTRKQYGPKVDLWSLGIMAIEMVEGEPPYLNENPLKALYLIATNGKPEIKEKDKLSSAF 790
            |||||||.:.:||.|||:||||||.|||:|.|||||.|:|::||||||.||.|.:|:.:.:|...
pombe   475 WMAPEVVKQNEYGTKVDIWSLGIMIIEMIENEPPYLREDPIRALYLIAKNGTPTLKKPNLVSKNL 539

  Fly   791 QDFLDQCLEVEVDRRASALDLLKHPFLKLARPLASLTPLIMAAKEATKGN 840
            :.||:.||.::...||:|.:||.|.||..|.|...|..:|.:.|..|..|
pombe   540 KSFLNSCLTIDTIFRATAAELLTHSFLNQACPTEDLKSIIFSRKANTHIN 589

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Pak	NP_001138013.2	PBD	83..137	CDD:279166	23/53 (43%)
		STKc_PAK_I	558..818	CDD:270814	143/265 (54%)
		S_TKc	566..817	CDD:214567	140/256 (55%)
shk2	NP_592864.1	PH_Cla4_Ste20	24..120	CDD:270097
		PBD	128..182	CDD:279166	23/53 (43%)
		STKc_PAK	308..567	CDD:270789	141/258 (55%)
		S_TKc	309..566	CDD:214567	140/256 (55%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000763
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100484
Panther	1	1.100	-	-	O	PTHR48015
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	5	4.910