DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment msn and TNIK

DIOPT Version :9

Sequence 1:	NP_524679.3	Gene:	msn / 44030	FlyBaseID:	FBgn0010909	Length:	1504	Species:	Drosophila melanogaster
Sequence 2:	NP_055843.1	Gene:	TNIK / 23043	HGNCID:	30765	Length:	1360	Species:	Homo sapiens

Alignment Length:	1575	Identity:	687/1575 - (43%)
Similarity:	894/1575 - (56%)	Gaps:	309/1575 - (19%)

- Green bases have known domain annotations that are detailed below.

  Fly    16 SLDDIDLTALKDPAGIFELIEVVGNGTYGQVYKGRHTKTGQLAAIKVMDVTEDEEEEIKLEINVL 80
            |||:|||:||:||||||||:|:||||||||||||||.||||||||||||||.|||||||.|||:|
Human     9 SLDEIDLSALRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKVMDVTGDEEEEIKQEINML 73

  Fly    81 KKYSNHRNIATYYGAFIKKSPPGKDDQLWLVMEYCGAGSVTDLVKSTKGQSLKEEWIAYICREIL 145
            ||||:||||||||||||||:|||.|||||||||:|||||||||:|:|||.:||||||||||||||
Human    74 KKYSHHRNIATYYGAFIKKNPPGMDDQLWLVMEFCGAGSVTDLIKNTKGNTLKEEWIAYICREIL 138

  Fly   146 RGLSYLHSNKVIHRDIKGQNVLLTDNAEVKLVDFGVSAQLDRTIGRRNTFIGTPYWMAPEVIACD 210
            ||||:||.:|||||||||||||||:||||||||||||||||||:|||||||||||||||||||||
Human   139 RGLSHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACD 203

  Fly   211 ENPDATYDNRSDLWSLGITALEMAESQPPLCDLHPMRALFLIPRNSPPRLKSKKWSKKFHGFIDT 275
            ||||||||.:||||||||||:||||..|||||:||||||||||||..||||||||||||..||::
Human   204 ENPDATYDFKSDLWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPAPRLKSKKWSKKFQSFIES 268

  Fly   276 VLVKDYHQRPYTENLLKHGFIKDQPTDRQVRIQLKDHIDRCKKRKQEKEREDYRYSGSDNDDDEP 340
            .|||::.|||.||.|:||.||:|||.:|||||||||||||.||::.||:..:|.||||:.:::|.
Human   269 CLVKNHSQRPATEQLMKHPFIRDQPNERQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEEEEEEN 333

  Fly   341 QLAGEHSSIVQAPGGDTLRRNFQQIQEG---------RLAAEQQQQHH------LMAQAQ----- 385
            . :||.|||:..||..||||:|.::|..         |...||||:.:      |:|:.|
Human   334 D-SGEPSSILNLPGESTLRRDFLRLQLANKERSEALRRQQLEQQQRENEEHKRQLLAERQKRIEE 397

  Fly   386 --AQAAAAHAAAQAQAQLQQQQQQAAAAAAAAAHAAQQ--AQQAAQQAQAQQPQANRQPKPPSRQ 446
              .|........:.:.:|::||::     ....|..:|  .::..::|:.:|....||.:...||
Human   398 QKEQRRRLEEQQRREKELRKQQER-----EQRRHYEEQMRREEERRRAEHEQEYIRRQLEEEQRQ 457

  Fly   447 ---------------------QVEEPGPPARPPQRLIVVPDPPHANRPLPPTPKCGEPAGQTPQQ 490
                                 |:||    .|..:||                            |
Human   458 LEILQQQLLHEQALLLEYKRKQLEE----QRQAERL----------------------------Q 490

  Fly   491 QQRNSQNNFKPSLPPRRPEPQP-------QTAAGGQGSQQQA---QPEAPPRNNRQSSGLSSSGG 545
            :|...:.::..||..:|.|.:|       ....|...|::.|   :.|...|.|||||
Human   491 RQLKQERDYLVSLQHQRQEQRPVEKKPLYHYKEGMSPSEKPAWAKEVEERSRLNRQSS------- 548

  Fly   546 SASGGGGSSKPAAALPQQSNNHLGQPVNPLDPLDSSDSDSEPDEPNDRARNDGTLLASDPPKP-- 608
                        .|:|.:..|.:..|..|......|.|..:|      ||....|...||..|
Human   549 ------------PAMPHKVANRISDPNLPPRSESFSISGVQP------ARTPPMLRPVDPQIPHL 595

  Fly   609 --LPGLGPVSEDANTTTPLSHGSGGPPNRPLPPTPDDDDQAGDRTLIMKRKLEQNINRLQKSAST 671
              :...||    |.|.:...|..         ||                   :.::..|::.:.
Human   596 VAVKSQGP----ALTASQSVHEQ---------PT-------------------KGLSGFQEALNV 628

  Fly   672 SQANVTPSRRGDESNLLRDWDFDRFFPKNANGPRGSGRGSPTTTASLSRSSQLSTLKVDTKLQRA 736
            :...|...|:..:             |.:.|.|      .||......|||.|..
Human   629 TSHRVEMPRQNSD-------------PTSENPP------LPTRIEKFDRSSWLRQ---------- 664

  Fly   737 SVAEAITRPVPRGYQPLKAEPSASQSIAKEQGSASGSGSGSGSASGSGSGGSTNSPAHKRQDSDS 801
              .|.|...||      :...|.|.::|::  ::.|:||..|...||....::| |..:|.:...
Human   665 --EEDIPPKVP------QRTTSISPALARK--NSPGNGSALGPRLGSQPIRASN-PDLRRTEPIL 718

  Fly   802 RLPTNFERGFRRENSDFFPLAKRYSAVFSGASASGSTAGS-PSAQALQRSSAVYQRNSIYSSSIS 865
            ..|                 .:|.|   ||:|:|.||..| ||:|...:..:  |..|...:.:.
Human   719 ESP-----------------LQRTS---SGSSSSSSTPSSQPSSQGGSQPGS--QAGSSERTRVR 761

  Fly   866 SKSKENDVPGAPGAAAAGTATASAKPGPAAATTTATKGAAPKTSKSLANFHFLRPRREKTESVIV 930
            :.||....|..|...|.                     ..|:.|:.:.     ||.|..:....:
Human   762 ANSKSEGSPVLPHEPAK---------------------VKPEESRDIT-----RPSRPASYKKAI 800

  Fly   931 LQNAAVRAQRLQQLQQQQQQQQLQQQQQQQNRGGGGGGGGGGGGGGSSGVGADGTGLGTPGTRTS 995
            .::....|:.|::|:.::..:.:::.....:        .......|.....||......||...
Human   801 DEDLTALAKELRELRIEETNRPMKKVTDYSS--------SSEESESSEEEEEDGESETHDGTVAV 857

  Fly   996 SVLPDLLSQASPATPPRHDKSSSEEYQAAISSSVHSTPSKSFIASSGSGGLGLGGGTVV---GGV 1057
            |.:|.|:...:|        .|:|:|...:..: |...:..  |.|.||.:...|..::   .|.
Human   858 SDIPRLIPTGAP--------GSNEQYNVGMVGT-HGLETSH--ADSFSGSISREGTLMIRETSGE 911

  Fly  1058 IISSNHSPQS----TISLAS--SSSNSRQNSPKNSISK--TRSSSSITNLLHKSASSSSAN---- 1110
            ...|.||..:    .|:|..  ..|:|...:|...:.:  |.|....:...:...||:.|:
Human   912 KKRSGHSDSNGFAGHINLPDLVQQSHSPAGTPTEGLGRVSTHSQEMDSGTEYGMGSSTKASFTPF 976

  Fly  1111 ----LHHLTPC-------SSTSSASISNPLPPHAYALQQKQRSFLTFGFGAGGSGPSRRESHVNV 1164
                ::..:|.       .|:::|..::.|      |:|:|...          ..:|:.|.|||
Human   977 VDPRVYQTSPTDEDEEDEESSAAALFTSEL------LRQEQAKL----------NEARKISVVNV 1025

  Fly  1165 NVTPTSHEAANDTPEIRKYKKRFNSEILCAALWGVNLLIGTENGLMLLDRSGQGKVYQLISRRRF 1229
            |  ||:....:|||||||||||||||||||||||||||:||||||||||||||||||.||:||||
Human  1026 N--PTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLINRRRF 1088

  Fly  1230 QQMEVLEGQNILVTISGKKNRVRVYYLSWLKSKILRTDGLSDQVERRNGWINVGDLQGAVHFKIV 1294
            |||:||||.|:|||||||||::||||||||:::||..|   .:||::.|||.||||:|.:|:|:|
Human  1089 QQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHND---PEVEKKQGWITVGDLEGCIHYKVV 1150

  Fly  1295 KYERIKFLVIALKDSIEIYAWAPKPYHKFMAFKNFGELEHRPLLVDLTIEDQSRLKVIYGSAEGF 1359
            |||||||||||||:::|||||||||||||||||:|.:|:|:|||||||:|:..|||||:||..||
Human  1151 KYERIKFLVIALKNAVEIYAWAPKPYHKFMAFKSFADLQHKPLLVDLTVEEGQRLKVIFGSHTGF 1215

  Fly  1360 HAVDLDSAEVYDIYLPKHTQGAIIPHCIVALPNSNGMQLLLCYDNEGVYVNTVGRVSKNIVLQWG 1424
            |.:|:||...||||:|.|.||.|.||.||.||.::||::|:||::|||||||.||::|::|||||
Human  1216 HVIDVDSGNSYDIYIPSHIQGNITPHAIVILPKTDGMEMLVCYEDEGVYVNTYGRITKDVVLQWG 1280

  Fly  1425 EMPTSVAYIGTGQIMGWGNKAIEIRSVESGHLDGVFMHKKAQRLKFLCERNDKVFFSSAKGASSC 1489
            |||||||||.:.||||||.|||||||||:||||||||||:||||||||||||||||:|.:...|.
Human  1281 EMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSS 1345

  Fly  1490 QIYFMTLNKPGMANW 1504
            |::|||||:..|.||
Human  1346 QVFFMTLNRNSMMNW 1360

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
msn	NP_524679.3	STKc_myosinIII_N_like	25..296	CDD:270785	230/270 (85%)
		S_TKc	32..296	CDD:214567	224/263 (85%)
		CNH	1183..1484	CDD:214481	225/300 (75%)
TNIK	NP_055843.1	STKc_TNIK	18..313	CDD:270807	250/294 (85%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	284..347		36/63 (57%)
		Mediates interaction with NEDD4. /evidence=ECO:0000269\|PubMed:20159449	290..1047		217/976 (22%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	398..440		6/46 (13%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	539..589		17/74 (23%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	601..801		60/319 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	814..878		13/79 (16%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	908..927		4/18 (22%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	933..998		10/64 (16%)
		CNH	1042..1340	CDD:214481	225/300 (75%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165160354
Domainoid	1	1.000	478	1.000	Domainoid score	I423
eggNOG	1	0.900	-	-		E1_KOG0587
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	1136	1.000	Inparanoid score	I219
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG46069
OrthoDB	1	1.010	-	-		D246443at33208
OrthoFinder	1	1.000	-	-		FOG0001422
OrthoInspector	1	1.000	-	-		otm41041
orthoMCL	1	0.900	-	-		OOG6_101812
Panther	1	1.100	-	-	O	PTHR48015
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R6825
SonicParanoid	1	1.000	-	-		X885
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	15	14.800