DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment nmd and Spast

DIOPT Version :9

Sequence 1:	NP_001285801.1	Gene:	nmd / 44021	FlyBaseID:	FBgn0005322	Length:	369	Species:	Drosophila melanogaster
Sequence 2:	NP_001156342.1	Gene:	Spast / 50850	MGIID:	1858896	Length:	614	Species:	Mus musculus

Alignment Length:	317	Identity:	115/317 - (36%)
Similarity:	188/317 - (59%)	Gaps:	22/317 - (6%)

- Green bases have known domain annotations that are detailed below.

  Fly    43 PTSKNKKKAKVLAEEQLKRLAEQEGFKLRGQEFSDYELMIASHL---VVPADITVSWADIAGLDS 104
            |...||......|..:.|.|          :.|.:.:..:|:.:   :|.....|.:.||||.:.
Mouse   293 PNRTNKPSTPTTAVRKKKDL----------KNFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQEL 347

  Fly   105 VIQELRESVVLPIQHKDLFKHSKLWQAP-KGVLLHGPPGCGKTLIAKATAKEAGMRFINLDVAIL 168
            ..|.|:|.|:||....:||...:   || :|:||.||||.|||::|||.|.|:...|.|:..|.|
Mouse   348 AKQALQEIVILPSLRPELFTGLR---APARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASL 409

  Fly   169 TDKWYGESQKLTSAVFSLASRIEPCIIFIDEIDSFLRSRNMNDHEATAMMKTQFMMLWDGLSTNA 233
            |.|:.||.:||..|:|::|..::|.||||||:||.|..|...:|:|:..:||:|::.:||:.:..
Mouse   410 TSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQSAG 474

  Fly   234 NSTVIVMGATNRPQDLDKAIVRRMPAQFHIGLPSETQRKDILKLIL--QSEEVSQDVDLNRLSKL 296
            :..|:|||||||||:||:|::||...:.::.||:|..|..:||.:|  |...::|. :|.:|:::
Mouse   475 DDRVLVMGATNRPQELDEAVLRRFIKRVYVSLPNEETRLLLLKNLLCKQGSPLTQK-ELAQLARM 538

  Fly   297 TNGFSGSDLREMCRNASVYRMRQLITSRDPSATALDRNNVRITMDDLLGSHLKIKES 353
            |:|:|||||..:.::|::..:|:|...:..:.:|.:..|:|::  |...|..|||.|
Mouse   539 TDGYSGSDLTALAKDAALGPIRELKPEQVKNMSASEMRNIRLS--DFTESLKKIKRS 593

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
nmd	NP_001285801.1	AAA	132..266	CDD:214640	62/134 (46%)
nmd	NP_001285801.1	AAA	135..265	CDD:278434	60/129 (47%)
Spast	NP_001156342.1	Required for interaction with RTN1. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	1..298		1/4 (25%)
		Required for midbody localization. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	1..192
		Required for interaction with ATL1. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	1..78
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..48
		Required for nuclear localization. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	1..48
		Nuclear localization signal. /evidence=ECO:0000255\|HAMAP-Rule:MF_03021	4..11
		Required for interaction with SSNA1 and microtubules. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	48..85
		Nuclear export signal. /evidence=ECO:0000255\|HAMAP-Rule:MF_03021	57..65
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	91..112
		Sufficient for interaction with CHMP1B. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	110..194
		Required for interaction with microtubules. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	112..198
		MIT_spastin	114..193	CDD:239142
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	220..306		3/12 (25%)
		Sufficient for microtubule severing. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	226..614		115/317 (36%)
		Required for interaction with microtubules and microtubule severing. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	268..326		8/42 (19%)
		Nuclear localization signal. /evidence=ECO:0000255\|HAMAP-Rule:MF_03021	307..310		0/2 (0%)
		Required for interaction with microtubules. /evidence=ECO:0000250\|UniProtKB:Q9UBP0	308..310		0/1 (0%)
		P-loop_NTPase	339..>395	CDD:304359	27/58 (47%)
		AAA	372..508	CDD:214640	63/135 (47%)
		AAA	376..506	CDD:278434	60/129 (47%)
		Vps4_C	<578..610	CDD:286426	7/18 (39%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0464
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.810