Protein Alignment trio and Kalrn

DIOPT Version :10

• Sequence 1: NP_651960.2
• Gene: trio / 43974
• FlyBaseID: FBgn0024277
• Length: 2263
• Species: Drosophila melanogaster
• Sequence 2: XP_038944566.1
• Gene: Kalrn / 84009
• RGDID: 621865
• Length: 2989
• Species: Rattus norvegicus
• Alignment Length: 2352
• Identity: 1015/2352 - (43%)
• Similarity: 1465/2352 - (62%)
• Gaps: 226/2352 - (9%)

  Fly     2 DGQRARDLLTLLQERVVFLTGGRDRRGGPLLCFPATPRRDRLKPEDLRRLLSYLISIPSDAAKNL 66
            ||.:|.|:|.:|:|:|.|::||||:||||:|.|||....||::.||||:|::||.|:||:.....
  Rat    31 DGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKR 95

  Fly    67 GFTVIIDMRGNGNCSTNVKTILKVLQEHFSANIHNVVIIKPDNFWQKQRASISSHKYKFETTTVS 131
            ||||||||||:.  ...:|.:||.|||.|.|.||..:|||||||||||:.:..|.|:.|||:.||
  Rat    96 GFTVIIDMRGSK--WDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVS 158

  Fly   132 IESLNKIVESHQLTGDFEGQQLYDHQQWTDARLAIEDFFWQAGDMADRIDDLQEDLNRNDFAEDV 196
            :|.|.|:|:..|||.:|:|...|:|::|.:.||::|:||..|..:..|::||||.|.|.:|..||
  Rat   159 VEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDV 223

  Fly   197 PLARHAIDHHNEMRKKITKLPIEDLDMQGKKLLAKINAMAPPGGQAPTNSGGPDMDSGPSSAGQQ 261
            ..:|..||.|.:::||:.|.|:|:||.:|::||..|..           |.|        .:|:.
  Rat   224 EGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRC-----------SDG--------FSGRN 269

  Fly   262 SQPSQQSRSVGGNPDMSAAVNKALRQIELIHTGQEKLLMLWQHKKVKLDQCFQWRLFEQDCEKMF 326
            ..|        |:.|..:.|.|....::.:|:.::.|..:|..:|:|||||||.||||||.||||
  Rat   270 CIP--------GSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMF 326

  Fly   327 DWILHNRDVFQMSYVEIGHNYSVAKSLQDEHQKFAVASMNVSVNIDRILAVAARLIESQHYAAQH 391
            |||.||:::|..|:.|||.:|..|..||.:|..||:.|||..|||:||::||:||.|:.|||:|.
  Rat   327 DWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQ 391

  Fly   392 IKTLAQRLDRTWKDFAAGLDERTAVLQLSVLFHHKAEQYCNSVASWAAACQASQPLPSDIQSLET 456
            ||.::.:||:.||.|||.||||:.:|.:|.:||.||||:.:.|.:|...| :...|||::|.||.
  Rat   392 IKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMC-SEGGLPSEMQDLEL 455

  Fly   457 AIRTHQSLYEAMCQAYTEVHSTSKKLLYQLDHLVQVCNQPPPPGVPDHRKNSNNGSFNKYERQNP 521
            ||..||||||.:.||||||....|.||       .|..:|..||      ||        |....
  Rat   456 AIHHHQSLYEQVTQAYTEVSQDGKALL-------DVLQRPLSPG------NS--------ESLTA 499

  Fly   522 AADYSEGASHVLAVIHQILSHHRTLEAKWHQERLRLHQTLALRLFQEDVKQVLDWLKNHGEVFLR 586
            .|:||:....||.|:|::|.|.|.||:.|...::||||.|.|.:||:||:|||||::||||.||.
  Rat   500 TANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLS 564

  Fly   587 KNTGIGRNFHKARVYQTSHDNFENVAQNTYSNAQKLLAAADELARSGEADPNEIYSVARELELQV 651
            |:||:|::.|:||..|..||:||.||||||:||.|||.||::||::||.||.|||..||.||:::
  Rat   565 KHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRI 629

  Fly   652 GSFAERVEQRRRRLDMAVIFYTHEKDVTAWIDKLRTDVSTDETRLSQENLEGIERILQQYQRDQQ 716
            ..|..|||||:..|||:|.|:||.|::..|::.|:.:|..|   :..::::.::.:::|:|: ||
  Rat   630 QDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLED---VCADSVDAVQELIKQFQQ-QQ 690

  Fly   717 ESSVNVCLTTISQGEALLQEMRS--------LEYAD---------NTGSIAALEATLEKLNKQKV 764
            .::::..|..|.:||.|:|::||        .|..|         ::.||:.:|:.|::|:..:|
  Rat   691 TATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAVSNNKTPHSSSISHIESVLQQLDDAQV 755

  Fly   765 ELEELWSARKFRADLILRLRYFERDARLLSVQMEMWSEEL--QHADL-SRDYQKAEQLIRMHNES 826
            ::|||:..||.:.|:.|:||.||:....::.:::.|:|:|  |..|. :.|...|||.::.|.|.
  Rat   756 QMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTER 820

  Fly   827 VTEIQNATYEVVQQGQDLLQLF---ENAGFISMADATHTAQARIEYLLDFLREREIDLEEMSEAK 888
            ...:.|.|:||:||||||.|..   :.:|...:.:......|:::.||:||.|::.:||..:|..
  Rat   821 KLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDVDLAAQVQELLEFLHEKQHELELNAEQT 885

  Fly   889 RAKLEQAVQLCQFQNDANQVISWIRNGEAMLVASFVTPNSLQEAEQLRKGHEQFQIAIE------ 947
            ..:|||.:||...|.:..||:.||||||:||.||.|..:||.|||||::.|||||:|||
  Rat   886 HKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHAT 950

  Fly   948 ---KTHTSAVQVKYRADALINANHYDPLSIDEISDDVTKKWQQLVTYAEERHKLVTASINFYKTA 1009
               |||.||:||:.:|:||:.|.|||..:|.|.::.|...||||:...|:|.|||.||:.||||:
  Rat   951 SLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTS 1015

  Fly  1010 EQVCSVLDSLEREYKREDDWCDGGGGSDK------AQAIVQLISKHQEQKEAFLKACTLARRTAE 1068
            |||||||:|||:||:|::|||   ||.||      ...::.|:|||.|||||||||||||||.||
  Rat  1016 EQVCSVLESLEQEYRRDEDWC---GGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAE 1077

  Fly  1069 TFLKYANRSQ----QCYQYKGNCEGHVKSKLDKLLTQENQVLDYWTLRKKSLDQCQQFVLFERSA 1129
            .||||.:|:.    ....:....|..||:.|.:||.:||:||.:|||:|:.||||||:|:|||||
  Rat  1078 VFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSA 1142

  Fly  1130 KQAIEWIHNTGEAYLSSRSNLVGISKEETEGLLKEHNEFRSTAKETRERVKLLIQLADSLVEKGH 1194
            |||::||..|||.|||:.:: .|.:.|||:.||||:.|||..||:|:|:||||||||||.|||||
  Rat  1143 KQALDWIQETGEYYLSTHTS-TGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGH 1206

  Fly  1195 AHASDIKQWVASVDQRYKDFSNRMDSYCEQLEKSLGMSQQQLLLGSDGNSS-----ISSSSGDRH 1254
            .||::|::||.:||:.|:|||.||..|...|||:||::       ::.|..     |.:|..||
  Rat  1207 IHATEIRKWVTTVDKHYRDFSLRMGKYRYTLEKALGVN-------TEDNKDLELDIIPASLSDR- 1263

  Fly  1255 SDPTLEAKLNSSNKENKEINEEKRKSARRKEFIMAELMQTERAYVNDLATCIKCFLEEFRAG-KS 1318
                 |.||..:   |.|:|||||||||:||||||||:|||:|||.||..|::.:|.|..:| :.
  Rat  1264 -----EVKLRDA---NHEVNEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEE 1320

  Fly  1319 VPSALIGQEDVIFGNIKEIHHFHQKIFLRELEKYETMPEDVGHCFVTWASKFDMYVHYCKNKPTS 1383
            :|..::.:|.:|||||:||:.||..|||:||||||.:||||||||||||.||.|||.||||||.|
  Rat  1321 IPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDS 1385

  Fly  1384 NNLLVQHGGSFFEELQRRLEVDHPLPAYLIKPVQRITKYQLLLKDLLSCCEESHGEIKEGLEVML 1448
            |.|:::|.|:||:|:|:|..:.:.:.:||||||||:||||||||:||:||||..||:|:||||||
  Rat  1386 NQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVML 1450

  Fly  1449 NVPKKANDAMHLSLLENCDVSVDKLGEVVLQDAFQAWDTKQIIRKGRERRVFLFELYLLFAKEVK 1513
            :||||||||||:|:||..|.::|..||::||||||.||.|.:|||||||.:||||:.|:|:||:|
  Rat  1451 SVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIK 1515

  Fly  1514 ESN-VVKYQFKSKLMTTDMGITEHIEGDETKFAVWTGRSPMLSDCRIVLKATSLETKQIWVKKLR 1577
            :|: ..||.:|:||:|:::|:|||:|||..|||:|:||:|. ||.:.||||:::||||.|:|.:|
  Rat  1516 DSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPS-SDNKTVLKASNIETKQEWIKNIR 1579

  Fly  1578 EVMQETC--FSGT---SLTLPKSPAKHSGSSQRSS-RDLDEQLT-ENDHDRCSLASFGSGNTTDS 1635
            ||:||..  ..|.   .:.|||:|||...:|:|.. .|.|.|.. .:..|..|:||..|.||.:|
  Rat  1580 EVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVES 1644

  Fly  1636 DNKLGNQEATWVVADYIATSGSNELSVSKGQQVEIVEPPTAGEPDFCLVRLNPQHDDAAVQEGLV 1700
            |...|..|.|.|:.|:.| :.|:|||:..||.||::|.|:. .|.:||||..   :.:..|||||
  Rat  1645 DKLSGGCELTVVLQDFSA-AHSSELSIQVGQTVELLERPSE-RPGWCLVRTT---ERSPPQEGLV 1704

  Fly  1701 PVSVL---------------KPPPGSHKHGSGTAANAASSAGSQKSDMQDQSNRSKADALSSSTK 1750
            |.|.|               .....|:.|.||.....:.|..:.:|.....|..|     |...|
  Rat  1705 PSSTLCISHSRSSVEMDCFFPLVKDSYSHSSGENGGKSESVANLQSQPSLNSIHS-----SPGPK 1764

  Fly  1751 RRGFSGRNWLPL-MNRKGTDKPPSSKPLVKKPSEKNLRTPQKHAEELAEQQNQQPGASPATVLPS 1814
            |...:.:.||.. :.|..:.|...:   :||  :|.:|..:|.    .:..:.:||..   ..|.
  Rat  1765 RSTNTLKKWLTSPVRRLNSGKADGN---IKK--QKKVRDGRKS----FDLGSPKPGDE---TTPQ 1817

  Fly  1815 SQFPSSTQHPGGAGAGDYEPDEEVGLELPPPMK-----PIQE--PHLIANGPPAFAKDAKESSGN 1872
            .   .|.......|.|:.|||||....||||||     |.|:  ..|:|         |:::..:
  Rat  1818 G---DSADEKSKKGWGEDEPDEESHTPLPPPMKIFDNDPTQDEMSSLLA---------ARQAPTD 1870

  Fly  1873 MASPGKMDGNPLSEIEEIVKTTTEQHESNSRVDGG---GGVENASTNGHGRSHDNNDESHTAVSD 1934
            :.:...:    :|.||::||       |...::||   |.:::.:...:..........:.....
  Rat  1871 VPTAADL----VSAIEKLVK-------SKLTLEGGSYRGSLKDPTVCLNEGMAPPTPPRNLEEEQ 1924

  Fly  1935 KAAALKKRQCVFAELMSTEEAYVQDLHEIVNGYMTEINNTNSDIPMPEDLKGGKMKLVFNNIKDI 1999
            ||.||:.|..|..||:.||:.||:||..:|.|:|..|....    :|||:: ||.|:||.||..|
  Rat  1925 KAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKG----VPEDMR-GKEKIVFGNIHQI 1984

  Fly  2000 YEWHRDFFLRALRNCQKSPADLGPLIKRSATKFALYYTYCSNKPLSEYIVSAHYQYFDSIRQKLG 2064
            |:||:||||..|..|.:....|..|..:...|..:|..||.|||.|||||:.:..||:.::|::.
  Rat  1985 YDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEIN 2049

  Fly  2065 HRLDLSNLIIKPVQRITKYELLIKEIIKATEGAGLYKEVPMLQEAYQQMKVVVKTVNDMMVVLRS 2129
            .||.||:.:|||:||||||:||:|:.::.:|.|||  |...:::|.:.|.:|.|..||||.:.|
  Rat  2050 QRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGL--ECSDIEKAVELMCLVPKRCNDMMNLGR- 2111

  Fly  2130 LQDFDGEITAQGSLLMQGPMNCV-VDAAQKHR--ELQVFLFQQIIIFADIEKTKNQYASPIFKYR 2191
            ||.|:|.:||||.||.|.....: :||..:.|  |.:||||:||:||:::  .:....:|.:.::
  Rat  2112 LQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSEL--LRKGSLTPGYMFK 2174

  Fly  2192 SHIQLNHMQMKELGD---CRFQI--RSTDPKIPEMTIICQAASQENYAGWRDMLNKILQQQNDLI 2251
            ..|::|::.::|..|   |:|.:  |.|..:     :|.|||:.:....|...:|::|:.|.|.:
  Rat  2175 RSIKMNYLVLEEDVDDDPCKFALMNRETSER-----VILQAANPDIQQAWVQDINQVLETQRDFL 2234

  Fly  2252 FMLSNPLSTKNK 2263
            ..|.:|:..:.|
  Rat  2235 NALQSPIEYQRK 2246

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
trio	NP_651960.2	SEC14	13..152	CDD:214706	75/138 (54%)
		SPEC	314..>468	CDD:238103	83/153 (54%)
		SPEC	563..778	CDD:238103	97/231 (42%)
		SPEC	782..997	CDD:238103	93/229 (41%)
		SPEC	897..1119	CDD:238103	125/240 (52%)
		SPEC	1125..1226	CDD:197544	60/100 (60%)
		RhoGEF	1287..1455	CDD:459876	105/168 (63%)
		PH1_Kalirin_Trio_like	1462..1584	CDD:270060	69/122 (57%)
		SH3_Kalirin_1	1643..1707	CDD:212786	28/78 (36%)
		RhoGEF	1945..2121	CDD:459876	75/175 (43%)
		PH2_Kalirin_Trio_p63RhoGEF	2130..2260	CDD:270061	43/137 (31%)
Kalrn	XP_038944566.1	SEC14	42..180	CDD:214706	75/139 (54%)
		SPEC	193..413	CDD:238103	103/246 (42%)
		SPEC	314..539	CDD:238103	116/246 (47%)
		SPEC	540..769	CDD:238103	97/232 (42%)
		SPEC	894..1132	CDD:238103	125/240 (52%)
		SPEC	1138..1238	CDD:197544	60/100 (60%)
		RhoGEF	1287..1457	CDD:238091	106/169 (63%)
		PH1_Kalirin_Trio_like	1464..1586	CDD:270060	69/122 (57%)
		SH3_Kalirin_1	1652..1711	CDD:212786	28/63 (44%)
		SH3-RhoG_link	1708..1892	CDD:465196	49/223 (22%)
		RhoGEF	1935..2104	CDD:459876	75/175 (43%)
		PH2_Kalirin_Trio_p63RhoGEF	2109..2243	CDD:270061	44/141 (31%)
		SH3_Kalirin_2	2327..2385	CDD:212787
		I-set	2474..2568	CDD:400151
		Ig strand B	2491..2495	CDD:409353
		Ig strand C	2504..2508	CDD:409353
		Ig strand E	2534..2538	CDD:409353
		Ig strand F	2548..2553	CDD:409353
		Ig strand G	2561..2564	CDD:409353
		FN3	2572..2663	CDD:238020
		STKc_Kalirin_C	2693..2940	CDD:271017