Protein Alignment trio and plekhg4

DIOPT Version :10

• Sequence 1: NP_651960.2
• Gene: trio / 43974
• FlyBaseID: FBgn0024277
• Length: 2263
• Species: Drosophila melanogaster
• Sequence 2: NP_001128280.3
• Gene: plekhg4 / 779571
• XenbaseID: XB-GENE-5998210
• Length: 1921
• Species: Xenopus tropicalis
• Alignment Length: 1987
• Identity: 400/1987 - (20%)
• Similarity: 679/1987 - (34%)
• Gaps: 651/1987 - (32%)

  Fly   275 PDMSAAVNKALRQIELIHTGQ-----EKLLMLWQHKKVKLDQCFQ--WRLFEQDCEKMFDWILHN 332
            |::|......|..:|.|::|:     |..|::..      ||.|:  |           :.::|.
 Frog   137 PEVSYTSIFTLEWLEFINSGRMGTSLENCLLMCD------DQIFRVPW-----------ERVVHP 184

  Fly   333 RDVFQMS--------------YVEIGHNYS--VAKSLQDEHQKFAVASMNVSVNIDRILAVAARL 381
            ..:.:..              |:|..:..|  :.:.|.|||       :...||:|.:.:.|:..
 Frog   185 EFIDKAQPPDENASDKKTTGLYLECNNEMSQPLWRDLTDEH-------IETPVNVDYVSSEASST 242

  Fly   382 IESQ----------------------------------------HYAAQHIKTLAQR-------- 398
            ::::                                        :|.:|. ||:||:
 Frog   243 LDTEFQVKTSMSSSEIEGEYVQLSDISVPRLGPQMGSLTQSIALNYKSQQ-KTVAQQTQDKQNQV 306

  Fly   399 --LD-RTWKDFAAGLDERTAVLQL----SVLFHHKAEQYCN-----SVASWAAACQASQPLPSDI 451
              || ||:|......|....:..|    ...|.||.....|     .:...|.....|:......
 Frog   307 VFLDSRTYKTSVVSSDIMPTLCSLQPSTKATFSHKPTTQQNIDVQDKIVDLAFGESISKKHLKQE 371

  Fly   452 QSLETAIRTHQSLYEAMCQAYTEVHSTSKKLLY--------QLDHLVQVCNQPPPPGVPDHRKNS 508
            .|.......|..:...:.|...:.|....::..        ..|...|.|.||...|
 Frog   372 SSFSAGSIKHSFVETCITQNNHQKHEDISEITVPGKVSDTGDTDKCTQSCEQPSLQG-------- 428

  Fly   509 NNGSFNKYERQNPAADYSEGASHVLAVIHQILSHHRTLEAKWHQERLRLHQTLALRLFQEDVKQV 573
            .|.|.:...:|...:.:..|...|..|...:        .:...|..|..:|:::..||...|.:
 Frog   429 GNFSTSLSFQQESRSHFINGTEDVRVVTDAL--------QEPPAEHSRPQETVSVEYFQSSNKAI 485

  Fly   574 -------------------LDWLK-NHGEVFLRKNTGIGRNFHKARVYQTSHDNFENVAQNTYSN 618
                               :|..| ...|...::||.......|.|          |...:.||:
 Frog   486 PKDLIHYTITDKCDQSLPSMDTTKCQFEEQKSKENTADTEESPKNR----------NAGSHIYSS 540

  Fly   619 AQKLLAAA------------DELARSGE-ADPNEIYSVARELELQVGSFAERVEQRRR------- 663
            |:....|.            |....||| .|......|:|:||......|..||||.:
 Frog   541 AESSAFAVSVLQEGNNVLNLDVCKESGEQVDGVNKELVSRDLENMQLQNALSVEQREQEPKSDSH 605

  Fly   664 --------------RLDMAVIFYTHEKDVTAWIDKLRT--DVSTDETRLSQEN----------LE 702
                          .|.|.|:..:.:..:|     |.|  :|..||..|:..|          .|
 Frog   606 GKIYTMLNSEQVGTHLSMCVLSGSDKVPLT-----LETLHEVVEDEQDLADFNSVLSNGELPPSE 665

  Fly   703 GIER-----ILQQYQRDQQESSVNVCLTTISQGEALLQEMRSLEYADNTGSIAALEATLEKLNKQ 762
            .|.:     :..|.:|..|:...| |...:.|..||.:...:...     :|.|.:..||.|..:
 Frog   666 DISKTALGNLKDQEERTDQDPQEN-CQQNMCQTPALSRRSNAQPL-----TIMAQDVNLEVLLSE 724

  Fly   763 KVELEELWSARKFRADLILRLR-------------------YF----ERDARLLSVQMEMWSEEL 804
            .|.|... ..:..||.:|:..|                   ||    .:|.|.|.:.:.:.|...
 Frog   725 VVYLPGT-KDKSGRAVIIINTRNTAWLNPHCNASELGRLFLYFYSIPRKDCRSLGLTLLVDSRRC 788

  Fly   805 Q--------------------HADLSRDYQKAEQLIRMHNESVTEIQNATYEVVQQGQDLLQLFE 849
            .                    |..|    ...|:.:.:|.|....||   ::::...:.|.:..|
 Frog   789 SPVPALFKAFHIVQAAVPSCIHGVL----MLVEKDLALHVEKPHGIQ---FDLLTSTKSLHKHIE 846

  Fly   850 NAGFISMADATHTAQARIEY--LLDFLRER----EIDLEEMSEAKRAKLEQAVQLCQFQNDANQV 908
                        .:|..:|:  |..:..:.    .:.||.:.|..|       ..|.|..||
 Frog   847 ------------ASQLPLEFDGLFRYCHKDWVTFRMKLEHLQENCR-------NACAFLQDA--- 889

  Fly   909 ISWIRNGEAMLVASFVTPNSLQEAE-QLRKGHEQFQIAIEKTHTSAVQVKYRADALINANHYDPL 972
               |:|   :.:.|.  ||..::|: .|:|..|..::.:|.|.  .||::....|::..     |
 Frog   890 ---IKN---LHIDSL--PNKAEDAKLLLQKFGELMRMVLEDTR--LVQLQKEGGAILAR-----L 939

  Fly   973 SIDEISDDVTKKWQQLVTYAEER--------HKLVTASINFYKTAEQVCSVLDSLEREYKREDDW 1029
            ..:|....:|:.:::.|..|.|.        |.||..|....:..|.|.. .::.:.:::....|
 Frog   940 RKEESCVTLTEDYREAVDVAAELYNQVDEGVHSLVKVSNKRIQEMELVIE-FEAFQEQFQEVGSW 1003

  Fly  1030 CDGGGG---------SDKAQAIVQLISKHQEQKEAFLKACTLARRTAETFLKYANRSQQCYQYKG 1085
            .:..|.         .|..:.::|   ..::.||.::.||...||..:...|     .:.:|...
 Frog  1004 IEHVGERLLKECNTLEDSLEVLLQ---TQRQFKEFYVVACEYCRRGQDIMQK-----MEKWQDLS 1060

  Fly  1086 NCEGHV-KSKLDKLLTQENQVLDYWTLRKKSLDQCQQFVLFERSAKQAIEWIHNTGEAYLSSRSN 1149
            :.|..: :.||.:.   :.:|.|:|...::|..|.::.|...:...:|.||.       |....:
 Frog  1061 SSETQIHRLKLQRC---KEKVEDFWRRLEESRYQLEKSVTLYQFFDKAYEWA-------LERMRH 1115

  Fly  1150 LVGISKEE----------TEGLLK---EHNEFRSTAKETRERVKLLIQLADSL-VEKGHAHASDI 1200
            |...|.::          .|||.:   :|.|.      :.|:...:.:||..| :||.      .
 Frog  1116 LASFSLDDCSSPEMCLSAIEGLERYKIQHPEI------SEEKFMEMKELACELKIEKA------T 1168

  Fly  1201 KQWVASVDQRYKDFSNRMDSYCEQ----LEKSL---------------------GMSQQQLL--- 1237
            |||..:.            |.|::    |||.|                     ||.|::||
 Frog  1169 KQWKFAW------------SKCQEARHVLEKKLEAALRAKSMLSGEQSSPREADGMEQKELLESN 1221

  Fly  1238 ---------------------LG-----------------------SDGNS-----------SIS 1247
                                 ||                       ||..|           |:.
 Frog  1222 RNKYFFQSKPYNSPLCPRERRLGRVLCNRPEIGNTDDHTTRKASPTSDAGSTVTSVFRTRKMSLQ 1286

  Fly  1248 SSSGD-------------------------------------------------RHSDPT----- 1258
            |.||:                                                 |.|:||
 Frog  1287 SISGEDLSLDLLHIAPSGSSTPTVTPRPFTRRLLRKAQSFDLPGSEGPGYGCQRRLSEPTRRGNT 1351

  Fly  1259 ------LEAKLNS------SNKENKEINEEKRKSARR--------------KEFIMAELMQTERA 1297
                  ||.....      |.::.::.:.|...|.||              ...|:.|::.|||.
 Frog  1352 GVFIKGLEVSSTELVDRPCSPRQTRDWSAECMYSERRCSVSSSDGRSRSSKLRHIIDEMVTTERE 1416

  Fly  1298 YVNDLATCIKCFLEEFRAGKSVPSALIGQEDVIFGNIKEIHHFHQKIFLRELEKYETMPEDVGHC 1362
            ||..|....:.:..|... ..:|..|.|:..:||||::::..||.:.||:|||.....|..|.||
 Frog  1417 YVRSLWYISENYFPEMER-VDLPQDLRGKRGIIFGNLEKLRDFHSQYFLKELESCCNHPLRVSHC 1480

  Fly  1363 FVTWASKFDMYVHYCKNKPTSNNLLVQHGGSFFEELQRRLEVDHPLPAYLIKPVQRITKYQLLLK 1427
            |:....:|.||..|.||||.|:.||..||..||:..|..|.....|.:||:||:||::||.||||
 Frog  1481 FLRHKDQFGMYALYSKNKPRSDALLGSHGNIFFKNKQFLLGDKMDLASYLLKPIQRMSKYALLLK 1545

  Fly  1428 DLLSCCEESHGE----IKEGLEVMLNVPKKANDAMHLSLLENCDVSVDKLGEVVLQDAFQAWDTK 1488
            ||:..|||:..:    ::...|::....:..||.:.:..:.:|||::.:.|::|.||.|..|
 Frog  1546 DLIKECEEAQEQELAYLRAAEEMVKFQLRHGNDLLAMDAIRDCDVNLKEQGQLVRQDEFTVW--- 1607

  Fly  1489 QIIRKGRERRVFLFELYLLFAKEVK-ESNVVKYQFKSKLMTTDMGITEHIEGDETKFAVWTGRSP 1552
             :.||..:|.|||||..:||:|..: |..:..|.:|....|.|:|:||:......:|.:|..|..
 Frog  1608 -LGRKKCQRHVFLFEDLILFSKPKRIEGGLDVYIYKRSFKTADIGLTENSGDSGLRFEIWFRRRK 1671

  Fly  1553 MLSDCRIVLKATSLETKQIWVKKLREVMQETCFSGTSLTLPKSPAKHSGSSQRSSRDLDEQLTEN 1617
            . ||..| |:|::.|||..|...:.:::.                      |:::|:.:.::.|
 Frog  1672 S-SDTHI-LQASNPETKHAWTSDIAKILW----------------------QQATRNKEIRMQE- 1711

  Fly  1618 DHDRCSLASFGSGNTTDSDNKLGNQEATWVVADYI------ATSGSNELSV-------SKGQ-QV 1668
                  :.|.|.||....|.|..:........|||      .|..|..:|:       .:|| .:
 Frog  1712 ------MVSMGVGNKPFLDIKPSDAAINNRAVDYIMKGRGARTRASIAVSLFDHSDPYKRGQAPI 1770

  Fly  1669 EIVEPPTAGEPDFCLVRLNPQHDDAAVQEGLVPVSVLKPPPGSHKHGSG---TAANAASSAGSQK 1730
            ......:||.|....: |.|.:....|.:.|:               ||   |:....:|:..::
 Frog  1771 TCSSVSSAGGPSSSSL-LGPLNLHTYVNQSLL---------------SGVFSTSRPFDTSSCLEE 1819

  Fly  1731 SDMQDQSNRSKADALSSSTKR------RGFSGRNW-----LPLMNRKGTDKPPS--SKP--LVKK 1780
            .::::::|.|:....:.|::.      .|.||.:.     :|..|.......||  |.|  :.::
 Frog  1820 DELENETNSSQPSMTTESSESSQCMSGAGSSGSDSGCVSNIPQDNVSEDAGSPSDASAPYSIQER 1884

  Fly  1781 PSEKNLRTPQKHAEELAEQQ--NQQPG---ASPATVL 1812
            .:..:.|.|:....:...:|  :..|.   .||:|::
 Frog  1885 RNNSSSRFPRAEKSKFINRQYISANPSHICLSPSTIV 1921

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
trio	NP_651960.2	SEC14	13..152	CDD:214706
		SPEC	314..>468	CDD:238103	34/231 (15%)
		SPEC	563..778	CDD:238103	58/285 (20%)
		SPEC	782..997	CDD:238103	47/272 (17%)
		SPEC	897..1119	CDD:238103	49/240 (20%)
		SPEC	1125..1226	CDD:197544	22/118 (19%)
		RhoGEF	1287..1455	CDD:459876	64/171 (37%)
		PH1_Kalirin_Trio_like	1462..1584	CDD:270060	40/122 (33%)
		SH3_Kalirin_1	1643..1707	CDD:212786	15/77 (19%)
		RhoGEF	1945..2121	CDD:459876
		PH2_Kalirin_Trio_p63RhoGEF	2130..2260	CDD:270061
plekhg4	NP_001128280.3	SEC14	726..857	CDD:469559	23/150 (15%)
		SPEC	989..1193	CDD:413338	47/246 (19%)
		RhoGEF	1406..1568	CDD:459876	63/162 (39%)
		PH_puratrophin-1	1570..1705	CDD:270062	44/162 (27%)