Protein Alignment trio and kalrnb

DIOPT Version :10

• Sequence 1: NP_651960.2
• Gene: trio / 43974
• FlyBaseID: FBgn0024277
• Length: 2263
• Species: Drosophila melanogaster
• Sequence 2: NP_001314918.2
• Gene: kalrnb / 678652
• ZFINID: ZDB-GENE-060421-7244
• Length: 2306
• Species: Danio rerio
• Alignment Length: 2347
• Identity: 971/2347 - (41%)
• Similarity: 1409/2347 - (60%)
• Gaps: 262/2347 - (11%)

  Fly     2 DGQRARDLLTLLQERVVFLTGGRDRRGGPLLCFPATPRRDRLKPEDLRRLLSYLISIPSDAAKNL 66
            ||.:|.|:||:|:|:|.|::||||:||||:|.|||....||:|.||||:|::||.::||:.....
Zfish    35 DGIKAADVLTVLREKVAFVSGGRDKRGGPILTFPARSNHDRIKQEDLRKLVTYLSTVPSEDVSKR 99

  Fly    67 GFTVIIDMRGNGNCSTNVKTILKVLQEHFSANIHNVVIIKPDNFWQKQRASISSHKYKFETTTVS 131
            |||||:||||:.  ...:|.:||.|||.|.|.|...:|||||||||||:.:..|.|:.|||:.||
Zfish   100 GFTVIVDMRGSK--WDLIKPLLKTLQESFPAEICVALIIKPDNFWQKQKTNFGSAKFTFETSMVS 162

  Fly   132 IESLNKIVESHQLTGDFEGQQLYDHQQWTDARLAIEDFFWQAGDMADRIDDLQEDLNRNDFAEDV 196
            :|.|.|:|:..|||||.||...|||.:||:.|:::|:|...|..:..|:::|||.|:|.:.|...
Zfish   163 VEGLTKLVDPSQLTGDLEGTLEYDHVEWTELRVSLEEFTGGALHLLSRLEELQEVLSRQELASSA 227

  Fly   197 PLARHAIDHHNEMRKKITKLPIEDLDMQGKKLLAKINAMAPPGGQAPTNSGGPDMDSGPSSAGQQ 261
            ..||..::.|..:||.:.|.|:::||.:|::||.||.    .||.... |||.|..|..
Zfish   228 EEARKLLEDHARLRKTMAKAPVDELDQEGQRLLQKIR----DGGDGRL-SGGADFQSLV------ 281

  Fly   262 SQPSQQSRSVGGNPDMSAAVNKALRQIELIHTGQEKLLMLWQHKKVKLDQCFQWRLFEQDCEKMF 326
                         |.:||.::|       :...::.||..|.::|.:||||||.||:|||.||||
Zfish   282 -------------PKISALLDK-------LQVTRQHLLQGWHNRKQQLDQCFQLRLYEQDAEKMF 326

  Fly   327 DWILHNRDVFQMSYVEIGHNYSVAKSLQDEHQKFAVASMNVSVNIDRILAVAARLIESQHYAAQH 391
            :||.||:::|..::.:||.:|..|..||.:|..||:.|||..|||.||::||.||.|:.||||..
Zfish   327 EWIGHNKELFLQTHTDIGVSYQHAADLQTQHDHFAMNSMNAYVNISRIVSVATRLCEAGHYAATQ 391

  Fly   392 IKTLAQRLDRTWKDFAAGLDERTAVLQLSVLFHHKAEQYCNSVASWAAACQASQPLPSDIQSLET 456
            |:.::.:||..||.||:||:||:|:|.:|.:||.|:||:.:|:..|..:| ....||:..|.||.
Zfish   392 IQQISGQLDHDWKSFASGLEERSAILAMSSVFHQKSEQFLSSMEGWVKSC-GEVGLPTVTQELEL 455

  Fly   457 AIRTHQSLYEAMCQAYTEVHSTSKKLLYQLDHLVQVCNQPPPPGVPDHRKNSNNGSFNKYERQNP 521
            ||..||:|||.:..|||||....|.||       .|..:||||        :::||.      ..
Zfish   456 AIHNHQNLYEQVTTAYTEVSQKGKTLL-------DVLQRPPPP--------ADSGSL------TA 499

  Fly   522 AADYSEGASHVLAVIHQILSHHRTLEAKWHQERLRLHQTLALRLFQEDVKQVLDWLKNHGEVFLR 586
            .||||:....||.|:|:::..:|.||......:|||||.|.|.:||:||:|||||::||||.||.
Zfish   500 GADYSQAVRGVLEVVHEVVHQYRRLEGLLQHHKLRLHQRLQLCVFQQDVQQVLDWIENHGEAFLS 564

  Fly   587 KNTGIGRNFHKARVYQTSHDNFENVAQNTYSNAQKLLAAADELARSGEADPNEIYSVARELELQV 651
            |:||:|::.|:||..|..||:|:.||||||:||.|||.||::||::||.||.|||:.|..||::|
Zfish   565 KHTGVGKSVHRARALQKRHDDFQEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYAAAHHLEVRV 629

  Fly   652 GSFAERVEQRRRRLDMAVIFYTHEKDVTAWIDKLRTDVSTDETRLSQENLEGIERILQQYQRDQQ 716
            ..|..|||||:..||::|.|:||.|::.:|::.|:.  |..|..::.|:::.::.:::|:|: ||
Zfish   630 QEFVRRVEQRKLLLDISVSFHTHTKELWSWMEDLQK--SLVEPVVNSESVDAVQELIRQFQQ-QQ 691

  Fly   717 ESSVNVCLTTISQGEALLQEMRSLEYADN----TGSIAALEATLEKLNKQKVELEELWSARKFRA 777
            .|:::..|..|.:||.|:|.:|....|.|    ..|:|.:|..|::|::.:..:|||:..|:.:.
Zfish   692 SSTLDATLNVIKEGEELIQHLRDSALACNKLPHASSVAHIEGVLQQLDEAQAHMEELFHERRIKL 756

  Fly   778 DLILRLRYFERDARLLSVQMEMWSEEL--QHADL-SRDYQKAEQLIRMHNESVTEIQNATYEVVQ 839
            |:.|:||.||:.|..:..:::.|.::|  |..|. :.:...|||.:..|.|....:.|.||||:|
Zfish   757 DIFLQLRIFEQYALEVMGELDAWKQDLMRQATDFNTEELTLAEQRLHRHTERKLAMNNMTYEVIQ 821

  Fly   840 QGQDLLQLF---ENAGFISMADATHTAQARIEYLLDFLREREIDLEEMSEAKRAKLEQAVQLCQF 901
            |||||.|..   :.:|.....:......|:::.||:||.|::.:||..:|....:|||.:||...
Zfish   822 QGQDLHQYIMEVQASGMELTGEKDVDLAAKVQELLEFLNEKQHELEVSAEHTHKRLEQCLQLRHL 886

  Fly   902 QNDANQVISWIRNGEAMLVASFVTPNSLQEAEQLRKGHEQFQIAIE---------KTHTSAVQVK 957
            |.:..||:.||||||:||.||......|.|.|||::.|||||:|||         :||.||:|::
Zfish   887 QAEVKQVLGWIRNGESMLTASTTNAGPLSEVEQLQREHEQFQLAIESLLHADSLQRTHQSALQLQ 951

  Fly   958 YRADALINANHYDPLSIDEISDDVTKKWQQLVTYAEERHKLVTASINFYKTAEQVCSVLDSLERE 1022
            .||:.|:.|.||||.::...::.|...||.|:...|:|.|||.||:.||||:||||:||:|||.|
Zfish   952 QRAELLLQAGHYDPDAVRACAETVALHWQTLMLKMEDRLKLVNASVAFYKTSEQVCNVLESLELE 1016

  Fly  1023 YKREDDWCDGGGGSDKAQAIVQ------LISKHQEQKEAFLKACTLARRTAETFLKYANRSQ--- 1078
            |:||:|||   ||.||..:..:      ||:||.|||||||||||||||.||.||||.:|:.
Zfish  1017 YRREEDWC---GGHDKLGSTAETDHVSPLINKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSM 1078

  Fly  1079 -QCYQYKGNCEGHVKSKLDKLLTQENQVLDYWTLRKKSLDQCQQFVLFERSAKQAIEWIHNTGEA 1142
             ....:....|..||:.|.:||.:||:||.:||::|:.||||||:|||||.|||||:|:..|||.
Zfish  1079 PGVVTHNRATESQVKAILSELLQRENRVLHFWTMKKRRLDQCQQYVLFERHAKQAIDWLQETGEY 1143

  Fly  1143 YLSSRSNLVGISKEETEGLLKEHNEFRSTAKETRERVKLLIQLADSLVEKGHAHASDIKQWVASV 1207
            :||:.:: .|.:.|:.:.||||:.:||.:||:|:|:||||||||.|||||||.|.:::|:||::|
Zfish  1144 FLSTHTS-SGETSEKAQELLKEYEDFRVSAKQTQEKVKLLIQLAGSLVEKGHVHVTELKRWVSTV 1207

  Fly  1208 DQRYKDFSNRMDSYCEQLEKSLGMSQQQLLLGSDGNSSISSS---SGDRHSDPTLEAKLNSSNKE 1269
            |:||:|||.||..|...|:::||:.       |:.|..:...   :...|:||  |..||.   .
Zfish  1208 DRRYRDFSMRMGHYRCSLDRALGVC-------SEDNKDLELDIIPANLTHTDP--EVNLND---P 1260

  Fly  1270 NKEINEEKRKSARRKEFIMAELMQTERAYVNDLATCIKCFLEEFRAGKSVPSALIGQEDVIFGNI 1334
            :.|:||||:||||:||:|||||:|||||||.||..|::.|..|..:|..|||.:..:|.||||||
Zfish  1261 DHEVNEEKKKSARKKEYIMAELLQTERAYVRDLQECLETFFWEMTSGVEVPSGIANKEHVIFGNI 1325

  Fly  1335 KEIHHFHQKIFLRELEKYETMPEDVGHCFVTWASKFDMYVHYCKNKPTSNNLLVQHGGSFFEELQ 1399
            :||:.||..|||:|||.||.:||||||||||||.||.|||.||||||.|:.|:....|.||:|:|
Zfish  1326 QEIYDFHNNIFLKELENYEQLPEDVGHCFVTWADKFHMYVTYCKNKPDSSQLIQGQAGRFFDEIQ 1390

  Fly  1400 RRLEVDHPLPAYLIKPVQRITKYQLLLKDLLSCCEESHGEIKEGLEVMLNVPKKANDAMHLSLLE 1464
            ||..:.:.:.:|||||||||||||||||:||:||||..|||||||||||:|||:||||||:|:||
Zfish  1391 RRHGLANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGEIKEGLEVMLSVPKRANDAMHVSMLE 1455

  Fly  1465 NCDVSVDKLGEVVLQDAFQAWDTKQIIRKGRERRVFLFELYLLFAKEVKESN-VVKYQFKSKLMT 1528
            ..|.::...||::|||.||.||.|.:|||||:|.:||||:.|:|:||:|:|: ..||.:|::|:|
Zfish  1456 GFDENLAVQGELILQDTFQVWDPKSLIRKGRDRHLFLFEISLVFSKEMKDSSGRTKYVYKNRLLT 1520

  Fly  1529 TDMGITEHIEGDETKFAVWTGRSPMLSDCRIVLKATSLETKQIWVKKLREVMQETC--FSGT--- 1588
            :::|:|||||||..|||:|.||:| .||.:.||||:|:|.||.|::.:|||:||..  ..|.
Zfish  1521 SELGVTEHIEGDPCKFALWAGRTP-TSDNKTVLKASSIEVKQEWIRNIREVIQERTVHLKGALKE 1584

  Fly  1589 SLTLPKSPAKHSGSSQR-SSRDLDEQ-LTENDHDRCSLASFGSGNTTDSDNKLGNQEATWVVADY 1651
            .:..||:||:....|:| ::.|.|.| ...:..|..|:||..|.||.|||...|..|.|.|:.|:
Zfish  1585 PIHAPKTPARQRSISKRETTEDADSQGDASSQPDTISIASRTSQNTVDSDKLSGGCEQTVVLQDF 1649

  Fly  1652 IATSGSNELSVSKGQQVEIVEPPTAGE-PDFCLVRLNPQHDDAAVQEGLVPVSVLKP-------- 1707
            :|.| ..|||:..||.||::|  .:|| |.:||||..   :::..||||||.|.|..
Zfish  1650 VAGS-VGELSIQSGQTVELLE--RSGERPGWCLVRTT---ENSPPQEGLVPSSTLSVSHSRSSVD 1708

  Fly  1708 -----PPGSHKHGSGTAANAASSAGSQKSDMQDQSNRSKADALSSSTKRRGFSG---RNWLP--- 1761
                 |||...:..|::...:.||.|    :|.|::.:.....|...||.|.:|   :.||.
Zfish  1709 MDCFFPPGKENYPVGSSEVKSESAAS----LQPQTSVTSLPTGSPGPKRSGTTGNTLKKWLTSPV 1769

  Fly  1762 --LMNRKGTDKPPSSKPLVKKPSEKNLRTPQKHAEELAEQQNQQPGASPATVLPSSQFPSSTQHP 1824
              |.:.||.:..      .|||:.|               |.::.|.....:.|..|  .||:..
Zfish  1770 RRLSHGKGDNAN------AKKPNNK---------------QRKRDGRKSVELGPHHQ--DSTEER 1811

  Fly  1825 GGAG---AGDYEPDEEVGLELPPPMKPIQEPHLIANGPPAFAKDAKESSGNMASPGKMDGNPLSE 1886
            |..|   .|:.|.::|....|||||:.|::|....:..|              .||...
Zfish  1812 GRRGTNSGGEEEAEDEPHTPLPPPMEIIKDPSAQEDKTP--------------EPGSFP------ 1856

  Fly  1887 IEEIVKTTTEQHESNSRVDGGGGVENASTNGHGRSHDNNDESHTAVSDKAAALKKRQCVFAELMS 1951
                                   |..:.|.......|...|      .|..||:.|..|..|::.
Zfish  1857 -----------------------VLGSDTPSDQTPRDPETE------QKNKALRGRMFVLNEMVQ 1892

  Fly  1952 TEEAYVQDLHEIVNGYMTEINNTNSDIPMPEDLKGGKMKLVFNNIKDIYEWHRDFFLRALRNCQK 2016
            ||..||:||...|.|:|..|....    :|||:. ||.|:||.||..||:||::|||..|..|.:
Zfish  1893 TERDYVKDLGVTVEGFMRRIGEKG----VPEDMT-GKDKIVFGNIHQIYDWHKEFFLCELEKCLQ 1952

  Fly  2017 SPADLGPLIKRSATKFALYYTYCSNKPLSEYIVSAHYQYFDSIRQKLGHRLDLSNLIIKPVQRIT 2081
            ....|..|..:...:..:|..||.|||.||::|:.:.:||:.::|::..||.:|:.:|||:||||
Zfish  1953 DHDKLAELFIKHERRLHMYVIYCQNKPRSEFVVAEYDKYFEGVQQEINSRLAISDYLIKPIQRIT 2017

  Fly  2082 KYELLIKEIIKATEGAGL-YKEVPMLQEAYQQMKVVVKTVNDMMVVLRSLQDFDGEITAQGSLLM 2145
            ||:||:|:.:|.:..||| .||:   ::|.:.|.:|.|..||||.:.| ||.::|::||||.||.
Zfish  2018 KYQLLLKDFLKYSTKAGLDCKEI---EKAVELMSLVPKQCNDMMNLGR-LQGYEGKLTAQGKLLQ 2078

  Fly  2146 QGPMNCVVD---AAQKHRELQVFLFQQIIIFADIEKTKNQYASPIFKYRSHIQLNHMQMKELGDC 2207
            |........   ...:.:|.:||||:||:||:::  .:...::|.::::..|:::::.|:|
Zfish  2079 QDTFFVTEQDSGVLSRSKERRVFLFEQIVIFSEL--LRKGSSTPGYQFKKSIKVSYLSMEE---- 2137

  Fly  2208 RFQIRSTDPKIPEMTIIC---------QAASQENYAGWRDMLNKILQQQNDLIFMLSNP------ 2257
              |:.| ||.  :..::|         |||:.:....|...:.::|..|.:.:..|.||
Zfish  2138 --QVES-DPY--KFVLLCRGSSERLTLQAANVDIKKEWVQSIRELLDMQINFLTALQNPQAALTS 2197

  Fly  2258 -LSTKNK 2263
             ||..|:
Zfish  2198 QLSNSNR 2204

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
trio	NP_651960.2	SEC14	13..152	CDD:214706	75/138 (54%)
		SPEC	314..>468	CDD:238103	75/153 (49%)
		SPEC	563..778	CDD:238103	94/218 (43%)
		SPEC	782..997	CDD:238103	86/229 (38%)
		SPEC	897..1119	CDD:238103	117/240 (49%)
		SPEC	1125..1226	CDD:197544	55/100 (55%)
		RhoGEF	1287..1455	CDD:459876	109/167 (65%)
		PH1_Kalirin_Trio_like	1462..1584	CDD:270060	65/122 (53%)
		SH3_Kalirin_1	1643..1707	CDD:212786	29/64 (45%)
		RhoGEF	1945..2121	CDD:459876	70/176 (40%)
		PH2_Kalirin_Trio_p63RhoGEF	2130..2260	CDD:270061	38/148 (26%)
kalrnb	NP_001314918.2	None