Protein Alignment trio and mcf2lb

DIOPT Version :10

• Sequence 1: NP_651960.2
• Gene: trio / 43974
• FlyBaseID: FBgn0024277
• Length: 2263
• Species: Drosophila melanogaster
• Sequence 2: XP_068079741.1
• Gene: mcf2lb / 569231
• ZFINID: ZDB-GENE-081104-416
• Length: 1104
• Species: Danio rerio
• Alignment Length: 1896
• Identity: 333/1896 - (17%)
• Similarity: 555/1896 - (29%)
• Gaps: 897/1896 - (47%)

  Fly     6 ARDLLTLLQERVVFLTGGRDRRGGPLLCFPATPRRDRLKPEDLRRLLSYLISIPSDAAKNLGFTV 70
            |.|::|.|:.:..||:|||.:.|.|::.||..|....:..::.|.:|:||.||||.:|..:||.|
Zfish    44 AADVITELKRQFAFLSGGRAQDGSPIIIFPEYPSFCEIGDQEFRNVLTYLTSIPSLSAAGVGFIV 108

  Fly    71 IIDMRGNGNCSTNVKTILKVLQEHFSANIHNVVIIKPDNFWQKQRASI--SSHKYKFETTTV--- 130
            :||.|.:.  .|.:|..|..:...|.||:..|::::|....|:..:.:  ..|:..|:...:
Zfish   109 VIDRRQDR--WTCLKGTLLRISGWFPANLRLVLVLRPSAILQRTLSDVFFKLHRDDFKVPVIMLS 171

  Fly   131 SIESLNKIVESHQLTGDFEGQQLYDHQQWTDARLAIEDFFWQAGDMADRIDDLQEDLNRNDFAED 195
            |:..|:..:|.:|||.:..|.|.|.|:.|...|..:|.|
Zfish   172 SVADLHTYIERNQLTQELGGSQYYCHKTWISHRTDLESF-------------------------- 210

  Fly   196 VPLARHAIDHHNEMRKKITKLPIEDLDMQGKKLLAKINAMAPPGGQAPTNSGGPDMDSGPSSAGQ 260
                                                                             
Zfish   211 ----------------------------------------------------------------- 210

  Fly   261 QSQPSQQSRSVGGNPDMSAAVNKALRQIELIHTGQEKLLMLWQHKKVKLDQCFQWRLFEQDCEKM 325
                                                                             
Zfish   211 ----------------------------------------------------------------- 210

  Fly   326 FDWILHNRDVFQMSYVEIGHNYSVAKSLQDEHQKFAVASMNVSVNIDRILAVAARLIESQHYAAQ 390
                                                                           |.
Zfish   211 ---------------------------------------------------------------AA 212

  Fly   391 HIKTLAQRLDRTWKDFAAGLDERTAVLQLSVLFHHKAEQYCNSVASWAAACQASQPLPSDIQSLE 455
            .:|.:||||....::.                                    |...|||:..:..
Zfish   213 LVKRMAQRLQVFGREL------------------------------------AETELPSNHLTAS 241

  Fly   456 TAIRTHQSLYEAMCQAYTEVHSTSKKLLYQLDHLVQVCNQPPPPGVPDHRKNSNNGSFNKYERQN 520
            :.:.||                ||:|
Zfish   242 SLLNTH----------------TSRK--------------------------------------- 251

  Fly   521 PAADYSEGASHVLAVIHQILSHHRTLEAKWHQERLRLHQTLALRLFQEDVKQVLDWLKNHGEVFL 585
                                                       .:|:||:...|
Zfish   252 -------------------------------------------DIFKEDMAGAL----------- 262

  Fly   586 RKNTGIGRNFHKARVYQTSHDNFENVAQNTYSNAQKLLAAADELARSGEADPNEIYSVARELELQ 650
                                           |..:|:|
Zfish   263 -------------------------------SQGRKIL--------------------------- 269

  Fly   651 VGSFAERVEQRRRRLDMAVIFYTHEKDVTAWIDKLRTDVSTDETRLSQENLEGIERILQQYQRDQ 715
                                                                  |.|.:..:||
Zfish   270 ------------------------------------------------------ENIREPVRRD- 279

  Fly   716 QESSVNVCLTTISQGEALLQEMRSLEYADNTGSIAALEATLEKLNKQKVELEELWSARKFRADLI 780
            .:||:|                     .|...::|.:...|.:||:.....:|.|...:.:.:|.
Zfish   280 PDSSLN---------------------PDQLENLATVHRLLSQLNESSTAFDEFWIQHQNKLELC 323

  Fly   781 LRLRYFERDARLLSVQMEMWSEELQHADLSRDYQKAEQLIRMHNESVTEIQNATYEVVQQGQDLL 845
            |::..||.:.:.|.                                 ||:..||        :.|
Zfish   324 LKVCQFEHNFQQLR---------------------------------TELDRAT--------ETL 347

  Fly   846 QLFENAGFISMADATHTAQARIEYLLDFLREREIDLEEMSEAKRAKLEQAVQLCQFQNDANQVIS 910
            ..|...|.         :.|:.|:||..|...|                 .::|:.   .::|.|
Zfish   348 NAFSAVGI---------SPAQTEHLLQELTNHE-----------------KKVCEV---LDRVRS 383

  Fly   911 WIRNGEAMLVASFVTPNSL-QEAEQLRKGHEQFQIAIEKTHTSAVQVKYRADALINANHYDPLSI 974
            .:..|:.::.:|.:..:|: .:..:|.|..|..                               .
Zfish   384 LVAEGQGLIDSSQILDDSIAAKCSELEKASENL-------------------------------T 417

  Fly   975 DEISDDVTKKWQQLVTYAEERHKLVTASINFYKTAEQVCSVLDSLEREYKREDDWCDGGGGSDKA 1039
            .|:.|..||               :|.:::.:|..:.:|.              |||.|
Zfish   418 QELKDKQTK---------------LTQAMDLHKRLDMMCK--------------WCDDG------ 447

  Fly  1040 QAIVQLISKHQEQKEAFLKACTLARRTAETFLKYANRSQQCYQYKGNCEGHVKSKLDKLLTQENQ 1104
                                                                             
Zfish   448 ----------------------------------------------------------------- 447

  Fly  1105 VLDYWTLRKKSLDQCQQFVLFERSAKQAIEWIHNTGEAYLSSRSNLVGISKEETEGLLKEHNEFR 1169
               .:.|..:.||:||.    :..|:.|:..:    |.||.:.:..   .|.|...:.:|::...
Zfish   448 ---IYMLASQPLDKCQS----QEGAESALSEL----ECYLETANEK---QKWEISTVWQEYDNIL 498

  Fly  1170 STAKETRERVKLLIQLADSLVEKGHAHASDIKQWVASVDQRYKDFSNRMDSYCEQLEKSLGMSQQ 1234
            :  .|.||:|....:...||.|........:|:..|...:..:..:...:|    |........:
Zfish   499 N--NELREQVTRAFEKKASLQEMFDRRRVSLKKLAAKQTRPVQPVAPSPES----LSSPAHRDHE 557

  Fly  1235 QLLLGSDGNS-----SISSSSGDRHSDPTLEAKLNSSNKENKEINEEKRKSARRKEFIMAELMQT 1294
            .:.:..|.:|     .|:|...||.|             .|..::||:...|..:..:|.||::|
Zfish   558 SICISEDSDSRGSCKQINSDQVDRSS-------------RNASVSEEEETLAVLRRHVMNELLET 609

  Fly  1295 ERAYVNDLATCIKCFLEEFRA-------GKSVPSALIGQEDVIFGNIKEIHHFHQKIFLRELEKY 1352
            |||||.:|    .|.|:.:.|       ...:|:||..::||:|||::||::||::|||||||.|
Zfish   610 ERAYVEEL----MCVLQGYAAEMDNPSMAPLIPAALQNKKDVLFGNMQEIYNFHKRIFLRELETY 670

  Fly  1353 ETMPEDVGHCFVTWASKFDMYVHYCKNKPTSNNLLVQHGG-SFFEELQRRLEVDHPLPAYLIKPV 1416
            ...||.||.||:.|..:..:|..||.|||.|.:|..|... :||:|.|::||....|.:||:|||
Zfish   671 TDYPELVGRCFLDWMGELQIYEKYCHNKPRSESLWRQCSDCAFFQECQKKLEHKLGLDSYLLKPV 735

  Fly  1417 QRITKYQLLLKDLLSCCEESHG--EIKEGLEVMLNVPKKANDAMHLSLLENCDVSVDKLGEVVLQ 1479
            |||||||||:|:::...:...|  |::..|..:|.:.|..||:|||..:...|.::..||.:::|
Zfish   736 QRITKYQLLIKEMIKYSKGCEGSVELQAALSSILGILKAVNDSMHLIAITGYDGNLGDLGRLLMQ 800

  Fly  1480 DAFQAW--------DTKQIIR-KGRERRVFLFELYLLFAKEVKES-----NVVKYQFKSKLMTTD 1530
            .:|..|        ...::.| |..:|.:||.|..|||.|..:||     ....|.||.:|....
Zfish   801 GSFSVWAEHKRGHVKVMELARFKPMQRHLFLHEKALLFCKRREESGEGYEKAPSYSFKQELSMAA 865

  Fly  1531 MGITEHIEGDETKFAVWTGRSPMLSDCRIVLKATSLETKQIWVKKLREVMQ---ETCFSGTSLTL 1592
            :|||||.:||..||.:|:..    .|....::|.|.|.|.|||.::|:::.   |.|    ...:
Zfish   866 IGITEHAKGDSKKFEIWSSS----RDEVYTIQAASEEVKTIWVTEIRKLLTGQLEAC----KERI 922

  Fly  1593 PKSPAKHSGSSQRSSRDLDEQLTENDHDRCSLASFGSGNTTDSDNKLGNQEATWVVADYIATSGS 1657
            .:.| |:..|.||:    .||.|               :.:.|.|:|...|.:.:.:|.:.....
Zfish   923 FEEP-KNEASQQRA----PEQFT---------------SESSSTNRLVRNERSSLKSDGVEKQKR 967

  Fly  1658 NELSVSKGQQVEIVEPPTAGEPDFCLVRLNPQHDDAAVQEGLVPVSVLKPPPGSHKHGSGTAANA 1722
            .|   .:|:::|                                 |:.:         |.||..|
Zfish   968 EE---ERGKELE---------------------------------SIFE---------SRTAERA 987

  Fly  1723 ASSAGSQKSDMQDQSNRSKADALSSSTKRRGFSGRNWLPLMN-----RKGTDKPPSSKPLVKKPS 1782
            ..|:.|.::..:.|..||....|.:. ......|..|....:     |:||
Zfish   988 KGSSFSFETKPKRQDVRSDPTPLETG-PHPNLGGVRWFSTSSLFQSRRRGT-------------- 1037

  Fly  1783 EKNLRTPQKHAEELAEQQNQQPGASPATVLPSSQFPSSTQHPGGAGA---GDYEPDEE----VGL 1840
                     |.|.....:.            |....:|.:|.|...|   .:.:|:||    :..
Zfish  1038 ---------HTEYTCWMKG------------SLSLDASVEHDGYFSAEEQANSDPEEEREDKLSE 1081

  Fly  1841 ELPPPMKPIQE 1851
            |.|.|::..:|
Zfish  1082 EEPQPVRIDEE 1092

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
trio	NP_651960.2	SEC14	13..152	CDD:214706	44/143 (31%)
		SPEC	314..>468	CDD:238103	12/153 (8%)
		SPEC	563..778	CDD:238103	21/214 (10%)
		SPEC	782..997	CDD:238103	29/215 (13%)
		SPEC	897..1119	CDD:238103	23/222 (10%)
		SPEC	1125..1226	CDD:197544	18/100 (18%)
		RhoGEF	1287..1455	CDD:459876	76/177 (43%)
		PH1_Kalirin_Trio_like	1462..1584	CDD:270060	42/138 (30%)
		SH3_Kalirin_1	1643..1707	CDD:212786	6/63 (10%)
		RhoGEF	1945..2121	CDD:459876
		PH2_Kalirin_Trio_p63RhoGEF	2130..2260	CDD:270061
mcf2lb	XP_068079741.1	CRAL_TRIO_2	66..197	CDD:463965	40/132 (30%)
		SPEC	328..>473	CDD:413338	43/356 (12%)
		RhoGEF	599..776	CDD:238091	76/180 (42%)
		PH-like	783..914	CDD:473070	41/134 (31%)