DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment trio and mcf2a

DIOPT Version :9

Sequence 1:	NP_651960.2	Gene:	trio / 43974	FlyBaseID:	FBgn0024277	Length:	2263	Species:	Drosophila melanogaster
Sequence 2:	XP_021328504.1	Gene:	mcf2a / 557201	ZFINID:	ZDB-GENE-030616-52	Length:	1111	Species:	Danio rerio

Alignment Length:	1137	Identity:	266/1137 - (23%)
Similarity:	430/1137 - (37%)	Gaps:	329/1137 - (28%)

- Green bases have known domain annotations that are detailed below.

  Fly   740 LEYADNTGSIAALEATLEKLNKQKVELEELWSARKFRADLILR--LRYFERDARLLSVQMEMWSE 802
            |.|.|.....:....||:..:..       |        ::||  :..|....:.::..::.:..
Zfish   174 LRYIDENQLTSEFGGTLDYCHSD-------W--------IVLRTAIESFAVMVKDIAQMLQAFGT 223

  Fly   803 ELQHADLSRDYQKAEQLIRMHNESVTEIQNATYEVVQQGQDLLQLFENA---GFISMA-DATHTA 863
            ||....||.:....|.|:..|.|....:::|...|:::|:.||...|.:   |.:... |.|...
Zfish   224 ELAETQLSEECSAIEFLLLSHTEKYRRLKDAIRSVMREGRQLLSNLETSRKEGDVDTRWDITQDW 288

  Fly   864 QARIEYLLDFLREREIDLEEMSEAKRAKLEQAVQLCQFQNDANQVISWIRNGEAMLVASFVTPNS 928
            .. ::.||..|.:.||..:...:....||:|.:||.:::                        :|
Zfish   289 DT-MQRLLAQLTDMEIAFDGFFDKHHLKLQQYLQLLRYE------------------------HS 328

  Fly   929 LQEAEQLRKGHEQFQIAIEKTHTSAVQVKYRADALINANHYDPLSIDEISDDVTKKWQQLVTYAE 993
            .||.|          .::||..                                         |:
Zfish   329 FQEME----------CSLEKLR-----------------------------------------AQ 342

  Fly   994 ERH-KLVTASINFYKTAEQVCSVLDSLEREYKREDDWCDGGGGSDKAQAIV----QLISKHQEQK 1053
            ||. .::..|:|   ..||....||.|::..:.|         ..:||.::    ||.:.|....
Zfish   343 ERKISIIGESLN---QTEQSVRELDGLDKRAQEE---------MSRAQVLILHGHQLAAGHHYAM 395

  Fly  1054 EAFLKACTLARRTAETFLKYANRSQQCYQYKGNCEGHVKSKLDKLLTQENQVLDYWTLRKKSLDQ 1118
            ...::.|...|...:|                     :.|.|              :.::.||.|
Zfish   396 ALIIQRCNELRHQCDT---------------------LTSAL--------------STKRSSLTQ 425

  Fly  1119 CQQFVLFERSAKQAIEWIHNTGEAYLSSRSNLVGIS-KEETEGLLKEHNEFRSTAKET------- 1175
            .|  .|..| .::|..|..:  .|||.:...:.... ||..:..|::..:|:..|...
Zfish   426 AQ--TLLHR-LEEAQRWCDD--GAYLLANQQVEKCQCKEGAQTALRDIEKFQEAAPPLLTAGADV 485

  Fly  1176 --RERVKLLIQLADSLVEKGHAHASDIKQWVASVDQRYKDFSNRMDSYCEQLEKSLGMSQQQLLL 1238
              .|...:|.....|.:||.....|.::   |.:..|........|.:...:         ||:.
Zfish   486 LFLEYESVLTPYLQSHIEKTFQKYSSVQ---ALIQSRQICLKKMADKHIRPI---------QLVA 538

  Fly  1239 GSDGN-----SSISSSSGDRHS------DPTLEAKLNSSNKENKE----INEEKRKSA------- 1281
            ....|     |.:.|...|.:|      |..|..|..|....|..    |::.:|.|:
Zfish   539 PRPENPPRSKSPLFSPKHDFNSGLKFTFDLPLPGKRTSRKSPNSRKIEVIHDYQRSSSLPYSIDG 603

  Fly  1282 -----RRKEFIMAELMQTERAYVNDLATCIKCFLEEF---RAGKSVPSALIGQEDVIFGNIKEIH 1338
                 ..|..:|.||::|||.||.:|...:..:..|.   .....:|::|..:.||:|||:.:|:
Zfish   604 EDSSDLLKRHVMKELIETERIYVEELLAVLLGYRAEMDNPSLAPLLPTSLRNKRDVLFGNLPDIY 668

  Fly  1339 HFHQKIFLRELEKYETMPEDVGHCFVTWASKFDMYVHYCKNKPTSNNLLVQHGG-SFFEELQRRL 1402
            :||..:||::||.....||.||.||:.....|.:|..||:|||.|.:|..|... .||:|.|::|
Zfish   669 NFHSSVFLQDLESCLESPEAVGACFLERKDHFHLYECYCQNKPRSESLWRQFSDCPFFQECQKKL 733

  Fly  1403 EVDHPLPAYLIKPVQRITKYQLLLKDLL---SCCEESHGEIKEGLEVMLNVPKKANDAMHLSLLE 1464
            |....|.:||:||:||:||||||||:||   |.||.. .|::..||.||::.|..||:||...:.
Zfish   734 EHKLALDSYLLKPIQRLTKYQLLLKELLKYSSGCERV-SELQGALEAMLDLLKSVNDSMHQIAIT 797

  Fly  1465 NCDVSVDKLGEVVLQDAFQAW--------DTKQIIR-KGRERRVFLFELYLLFAKEVKE--SNVV 1518
            ..:..:..||.|::|.:|..|        ..|::.| |..:|.:||.|..|||.|..:|  .|..
Zfish   798 GYEGDLSDLGRVLMQGSFSVWIGHKKGPTRVKEMARFKPMQRHLFLHEHALLFCKRREEHGENAD 862

  Fly  1519 K---YQFKSKLMTTDMGITEHIEGDETKFAVW-TGRSPMLSDCRIVLKATSLETKQIWVKKLREV 1579
            |   |.||..|..:.:||||:::||..||.:| :||..:     .|::|.::|.|..|:.::|::
Zfish   863 KTPSYSFKHCLKMSAVGITENVKGDVKKFEIWYSGREEV-----YVIQAPTVEVKIAWLNEIRKI 922

  Fly  1580 M--------QETCFSGTSLTLPKSPAKHSGSSQRSSRDLDEQLTENDHDRCSLASFG--SGNTTD 1634
            :        :|.|.|             :..|::....:...|:.:...|.|::|.|  ||:::.
Zfish   923 LTNQQKQLKEELCLS-------------TAVSEQMQMQISPPLSVSKQQRASVSSDGTESGHSSP 974

  Fly  1635 SDNKLGNQE----ATWVVADYIAT--------SGSNELSVSKGQQVEIVEPPTAGEPDFCLVRLN 1687
            ......:|:    .:|..|.|...        ||:|.||          .|....|.|  .|.|.
Zfish   975 DAFSCSSQQQRNRRSWPGAPYSVDICEGLEDWSGANSLS----------NPSDTEEED--AVHLV 1027

  Fly  1688 P---------------------------QHDD----------AAVQEGLVPVSVLKPPPGSHKHG 1715
            |                           ||::          :..|||.||...|:...|
Zfish  1028 PGRYKVFMDWRNNGLDNLMIKCGDVIQLQHEEDHGQWLVKNLSRRQEGRVPTPNLQCILG----- 1087

  Fly  1716 SGTAANAASSAGSQKSDMQDQSNRSKADALSS 1747
                    .|..:..|.:.||..| ||..|||
Zfish  1088 --------ESMKNHSSRLGDQGIR-KARKLSS 1110

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
trio	NP_651960.2	SEC14	13..152	CDD:214706
		SPEC	314..>468	CDD:238103
		SPEC	563..778	CDD:238103	6/37 (16%)
		SPEC	782..997	CDD:238103	38/221 (17%)
		SPEC	897..1119	CDD:238103	31/226 (14%)
		SPEC	1125..1226	CDD:197544	20/110 (18%)
		RhoGEF	1287..1456	CDD:279015	72/175 (41%)
		PH1_Kalirin_Trio_like	1462..1584	CDD:270060	42/144 (29%)
		PH	1495..1580	CDD:278594	32/90 (36%)
		SH3_Kalirin_1	1643..1707	CDD:212786	22/112 (20%)
		RhoGEF	1945..2122	CDD:279015
		PH2_Kalirin_Trio_p63RhoGEF	2130..2260	CDD:270061
mcf2a	XP_021328504.1	SEC14	46..191	CDD:214706	4/16 (25%)
		SPEC	324..520	CDD:321951	50/325 (15%)
		RhoGEF	611..788	CDD:238091	73/177 (41%)
		PH_Dbs	795..926	CDD:269934	41/135 (30%)
		SH3_DBS	1030..1084	CDD:212791	8/53 (15%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG4240
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	3	2.810