Protein Alignment trio and Kalrn

DIOPT Version :10

• Sequence 1: NP_651960.2
• Gene: trio / 43974
• FlyBaseID: FBgn0024277
• Length: 2263
• Species: Drosophila melanogaster
• Sequence 2: XP_011244253.1
• Gene: Kalrn / 545156
• MGIID: 2685385
• Length: 2999
• Species: Mus musculus
• Alignment Length: 2354
• Identity: 1021/2354 - (43%)
• Similarity: 1460/2354 - (62%)
• Gaps: 231/2354 - (9%)

  Fly     2 DGQRARDLLTLLQERVVFLTGGRDRRGGPLLCFPATPRRDRLKPEDLRRLLSYLISIPSDAAKNL 66
            ||.:|.|:|.:|:|:|.|::||||:||||:|.|||....||::.||||:|::||.|:||:.....
Mouse    36 DGLKASDVLPILKEKVAFVSGGRDKRGGPILTFPARSNHDRIRQEDLRKLVTYLASVPSEDVCKR 100

  Fly    67 GFTVIIDMRGNGNCSTNVKTILKVLQEHFSANIHNVVIIKPDNFWQKQRASISSHKYKFETTTVS 131
            ||||||||||:.  ...:|.:||.|||.|.|.||..:|||||||||||:.:..|.|:.|||:.||
Mouse   101 GFTVIIDMRGSK--WDLIKPLLKTLQEAFPAEIHVALIIKPDNFWQKQKTNFGSSKFIFETSMVS 163

  Fly   132 IESLNKIVESHQLTGDFEGQQLYDHQQWTDARLAIEDFFWQAGDMADRIDDLQEDLNRNDFAEDV 196
            :|.|.|:|:..|||.:|:|...|:|::|.:.||::|:||..|..:..|::||||.|.|.:|..||
Mouse   164 VEGLTKLVDPSQLTEEFDGSLDYNHEEWIELRLSLEEFFNSAVHLLSRLEDLQEMLARKEFPVDV 228

  Fly   197 PLARHAIDHHNEMRKKITKLPIEDLDMQGKKLLAKINAMAPPGGQAPTNSGGPDMDSGPSSAGQQ 261
            ..:|..||.|.:::||:.|.|:|:||.:|::||..|..           |.|        .:|:.
Mouse   229 EGSRRLIDEHTQLKKKVLKAPVEELDREGQRLLQCIRC-----------SDG--------FSGRN 274

  Fly   262 SQPSQQSRSVGGNPDMSAAVNKALRQIELIHTGQEKLLMLWQHKKVKLDQCFQWRLFEQDCEKMF 326
            ..|        |:.|..:.|.|....::.:|:.::.|..:|..:|:|||||||.||||||.||||
Mouse   275 CIP--------GSADFQSLVPKITSLLDKLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMF 331

  Fly   327 DWILHNRDVFQMSYVEIGHNYSVAKSLQDEHQKFAVASMNVSVNIDRILAVAARLIESQHYAAQH 391
            |||.||:::|..|:.|||.:|..|..||.:|..||:.|||..|||:||::||:||.|:.|||:|.
Mouse   332 DWISHNKELFLQSHTEIGVSYQHALDLQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYASQQ 396

  Fly   392 IKTLAQRLDRTWKDFAAGLDERTAVLQLSVLFHHKAEQYCNSVASWAAACQASQPLPSDIQSLET 456
            ||.::.:||:.||.|||.||||:.:|.:|.:||.||||:.:.|.:|...| :...|||::|.||.
Mouse   397 IKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKAEQFLSGVDAWCKMC-SEGGLPSEMQDLEL 460

  Fly   457 AIRTHQSLYEAMCQAYTEVHSTSKKLLYQLDHLVQVCNQPPPPGVPDHRKNSNNGSFNKYERQNP 521
            ||..||||||.:.||||||....|.||       .|..:|..||      ||        |....
Mouse   461 AIHHHQSLYEQVTQAYTEVSQDGKALL-------DVLQRPLSPG------NS--------ESLTA 504

  Fly   522 AADYSEGASHVLAVIHQILSHHRTLEAKWHQERLRLHQTLALRLFQEDVKQVLDWLKNHGEVFLR 586
            .|:||:....||.|:|::|.|.|.||:.|...::||||.|.|.:||:||:|||||::||||.||.
Mouse   505 TANYSKAVHQVLDVVHEVLHHQRRLESIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLS 569

  Fly   587 KNTGIGRNFHKARVYQTSHDNFENVAQNTYSNAQKLLAAADELARSGEADPNEIYSVARELELQV 651
            |:||:|::.|:||..|..||:||.||||||:||.|||.||::||::||.||.|||..||.||:::
Mouse   570 KHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRI 634

  Fly   652 GSFAERVEQRRRRLDMAVIFYTHEKDVTAWIDKLRTDVSTDETRLSQENLEGIERILQQYQRDQQ 716
            ..|..|||||:..|||:|.|:||.|::..|::.|:.:|..|   :..::::.::.:::|:|: ||
Mouse   635 QDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKEVLED---VCADSVDAVQELIKQFQQ-QQ 695

  Fly   717 ESSVNVCLTTISQGEALLQEMRS--------LEYAD---------NTGSIAALEATLEKLNKQKV 764
            .::::..|..|.:||.|:|::||        .|..|         ::.||:.:|:.|::|:..:|
Mouse   696 TATLDATLNVIKEGEDLIQQLRSAPPSLGEPTEARDSAMSNNKTPHSSSISHIESVLQQLDDAQV 760

  Fly   765 ELEELWSARKFRADLILRLRYFERDARLLSVQMEMWSEEL--QHADL-SRDYQKAEQLIRMHNES 826
            ::|||:..||.:.|:.|:||.||:....::.:::.|:|:|  |..|. :.|...|||.::.|.|.
Mouse   761 QMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLLRQMNDFNTEDLTLAEQRLQRHTER 825

  Fly   827 VTEIQNATYEVVQQGQDLLQLF---ENAGFISMADATHTAQARIEYLLDFLREREIDLEEMSEAK 888
            ...:.|.|:||:||||||.|..   :.:|...:.:......|:::.||:||.|::.:||..:|..
Mouse   826 KLAMNNMTFEVIQQGQDLHQYIMEVQASGIELICEKDLDLAAQVQELLEFLHEKQHELELNAEQT 890

  Fly   889 RAKLEQAVQLCQFQNDANQVISWIRNGEAMLVASFVTPNSLQEAEQLRKGHEQFQIAIE------ 947
            ..:|||.:||...|.:..||:.||||||:||.||.|..:||.|||||::.|||||:|||
Mouse   891 HKRLEQCLQLRHLQAEVKQVLGWIRNGESMLNASLVNASSLSEAEQLQREHEQFQLAIESLFHAT 955

  Fly   948 ---KTHTSAVQVKYRADALINANHYDPLSIDEISDDVTKKWQQLVTYAEERHKLVTASINFYKTA 1009
               |||.||:||:.:|:||:.|.|||..:|.|.::.|...||||:...|:|.|||.||:.||||:
Mouse   956 SLQKTHQSALQVQQKAEALLQAGHYDADAIRECAEKVALHWQQLMLKMEDRLKLVNASVAFYKTS 1020

  Fly  1010 EQVCSVLDSLEREYKREDDWCDGGGGSDK------AQAIVQLISKHQEQKEAFLKACTLARRTAE 1068
            |||||||:|||:||:|::|||   ||.||      ...::.|:|||.|||||||||||||||.||
Mouse  1021 EQVCSVLESLEQEYRRDEDWC---GGRDKLGPAAEMDHVIPLLSKHLEQKEAFLKACTLARRNAE 1082

  Fly  1069 TFLKYANRSQ----QCYQYKGNCEGHVKSKLDKLLTQENQVLDYWTLRKKSLDQCQQFVLFERSA 1129
            .||||.:|:.    ....:....|..||:.|.:||.:||:||.:|||:|:.||||||:|:|||||
Mouse  1083 VFLKYIHRNNVSMPSVASHTRGPEQQVKAILSELLQRENRVLHFWTLKKRRLDQCQQYVVFERSA 1147

  Fly  1130 KQAIEWIHNTGEAYLSSRSNLVGISKEETEGLLKEHNEFRSTAKETRERVKLLIQLADSLVEKGH 1194
            |||::||..|||.|||:.:: .|.:.|||:.||||:.|||..||:|:|:||||||||||.|||||
Mouse  1148 KQALDWIQETGEYYLSTHTS-TGETTEETQELLKEYGEFRVPAKQTKEKVKLLIQLADSFVEKGH 1211

  Fly  1195 AHASDIKQWVASVDQRYKDFSNRMDSYCEQLEKSLGMSQQQLLLGSDGNSS-----ISSSSGDRH 1254
            .||::|::||.:||:.|:|||.||..|...|||:||::       ::.|..     |.:|..||
Mouse  1212 IHATEIRKWVTTVDKHYRDFSLRMGKYRYSLEKALGVN-------TEDNKDLELDIIPASLSDR- 1268

  Fly  1255 SDPTLEAKLNSSNKENKEINEEKRKSARRKEFIMAELMQTERAYVNDLATCIKCFLEEFRAG-KS 1318
                 |.||..:   |.|||||||||||:||||||||:|||:|||.||..|::.:|.|..:| :.
Mouse  1269 -----EVKLRDA---NHEINEEKRKSARKKEFIMAELLQTEKAYVRDLHECLETYLWEMTSGVEE 1325

  Fly  1319 VPSALIGQEDVIFGNIKEIHHFHQKIFLRELEKYETMPEDVGHCFVTWASKFDMYVHYCKNKPTS 1383
            :|..::.:|.:|||||:||:.||..|||:||||||.:||||||||||||.||.|||.||||||.|
Mouse  1326 IPPGILNKEHIIFGNIQEIYDFHNNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDS 1390

  Fly  1384 NNLLVQHGGSFFEELQRRLEVDHPLPAYLIKPVQRITKYQLLLKDLLSCCEESHGEIKEGLEVML 1448
            |.|:::|.|:||:|:|:|..:.:.:.:||||||||:||||||||:||:||||..||:|:||||||
Mouse  1391 NQLILEHAGTFFDEIQQRHGLANSISSYLIKPVQRVTKYQLLLKELLTCCEEGKGELKDGLEVML 1455

  Fly  1449 NVPKKANDAMHLSLLENCDVSVDKLGEVVLQDAFQAWDTKQIIRKGRERRVFLFELYLLFAKEVK 1513
            :||||||||||:|:||..|.::|..||::||||||.||.|.:|||||||.:||||:.|:|:||:|
Mouse  1456 SVPKKANDAMHVSMLEGFDENLDVQGELILQDAFQVWDPKSLIRKGRERHLFLFEISLVFSKEIK 1520

  Fly  1514 ESN-VVKYQFKSKLMTTDMGITEHIEGDETKFAVWTGRSPMLSDCRIVLKATSLETKQIWVKKLR 1577
            :|: ..||.:|:||:|:::|:|||:|||..|||:|:||:|. ||.:.||||:::||||.|:|.:|
Mouse  1521 DSSGHTKYVYKNKLLTSELGVTEHVEGDPCKFALWSGRTPS-SDNKTVLKASNIETKQEWIKNIR 1584

  Fly  1578 EVMQETC--FSGT---SLTLPKSPAKHSGSSQRSS-RDLDEQLT-ENDHDRCSLASFGSGNTTDS 1635
            ||:||..  ..|.   .:.|||:|||...:|:|.. .|.|.|.. .:..|..|:||..|.||.:|
Mouse  1585 EVIQERIIHLKGALKEPIQLPKTPAKLRNNSKRDGVEDGDSQGDGSSQPDTISIASRTSQNTVES 1649

  Fly  1636 DNKLGNQEATWVVADYIATSGSNELSVSKGQQVEIVEPPTAGEPDFCLVRLNPQHDDAAVQEGLV 1700
            |...|..|.|.|:.|:.| ..|:|||:..||.||::|.|:. .|.:||||..   :.:..|||||
Mouse  1650 DKLSGGCELTVVLQDFSA-GHSSELSIQVGQTVELLERPSE-RPGWCLVRTT---ERSPPQEGLV 1709

  Fly  1701 PVSVL--------------KPPPGSHKHGSGTAANAASSAGSQKSDMQDQSNRSKADALSS-STK 1750
            |.|.|              .|...|:.|.|      :.:.|..:|....||..|.....|| ..|
Mouse  1710 PSSALCISHSRSSVEMDCFFPLKDSYSHSS------SENGGKSESVAHLQSQPSLNSIHSSPGPK 1768

  Fly  1751 RRGFSGRNWLPL-MNRKGTDKPPSSKPLVKKPSEKNLRTPQKHAEELAEQQNQQPGASPATVLPS 1814
            |...:.:.||.. :.|..:.|...:   :||  :|.:|..:|.    .:..:.:||..   ..|.
Mouse  1769 RSTNTLKKWLTSPVRRLNSGKADGN---IKK--QKKVRDGRKS----FDLGSPKPGDE---TTPQ 1821

  Fly  1815 SQFPSSTQHPGGAGAGDYEPDEEVGLELPPPMKPIQEPHLIANGPPAFAKDAKES--SGNMASPG 1877
            .   .|.......|.|:.|||||....||||||      :..|.|   .:|...|  :...|.|.
Mouse  1822 G---DSADEKSKKGWGEDEPDEESHTPLPPPMK------IFDNDP---TQDEMSSLLAARQAPPD 1874

  Fly  1878 KMDGNPL-SEIEEIVKTTTEQHESNSRVDGGGGVENASTNGHGRSHD---------NNDESHTAV 1932
            ......| |.||::||        |.....||....:..:..|..::         |.:|
Mouse  1875 VPTAADLVSAIEKLVK--------NKLTLEGGSYRGSLKDPTGCLNEGMTPPTPPRNLEE----- 1926

  Fly  1933 SDKAAALKKRQCVFAELMSTEEAYVQDLHEIVNGYMTEINNTNSDIPMPEDLKGGKMKLVFNNIK 1997
            ..||.||:.|..|..||:.||:.||:||..:|.|:|..|....    :|||:: ||.|:||.||.
Mouse  1927 EQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKG----VPEDMR-GKEKIVFGNIH 1986

  Fly  1998 DIYEWHRDFFLRALRNCQKSPADLGPLIKRSATKFALYYTYCSNKPLSEYIVSAHYQYFDSIRQK 2062
            .||:||:||||..|..|.:....|..|..:...|..:|..||.|||.|||||:.:..||:.::|:
Mouse  1987 QIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQE 2051

  Fly  2063 LGHRLDLSNLIIKPVQRITKYELLIKEIIKATEGAGLYKEVPMLQEAYQQMKVVVKTVNDMMVVL 2127
            :..||.||:.:|||:||||||:||:|:.::.:|.|||  |...:::|.:.|.:|.|..||||.:.
Mouse  2052 INQRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGL--ECSDIEKAVELMCLVPKRCNDMMNLG 2114

  Fly  2128 RSLQDFDGEITAQGSLLMQGPMNCV-VDAAQKHR--ELQVFLFQQIIIFADIEKTKNQYASPIFK 2189
            | ||.|:|.:||||.||.|.....: :||..:.|  |.:||||:||:||:::  .:....:|.:.
Mouse  2115 R-LQGFEGTLTAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSEL--LRKGSLTPGYM 2176

  Fly  2190 YRSHIQLNHMQMKELGD---CRFQI--RSTDPKIPEMTIICQAASQENYAGWRDMLNKILQQQND 2249
            ::..|::|::.:::..|   |:|.:  |.|..:     :|.|||:.:....|...:|::|:.|.|
Mouse  2177 FKRSIKMNYLVLEDNVDGDPCKFALMNRETSER-----VILQAANSDIQQAWVQDINQVLETQRD 2236

  Fly  2250 LIFMLSNPLSTKNK 2263
            .:..|.:|:..:.|
Mouse  2237 FLNALQSPIEYQRK 2250

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
trio	NP_651960.2	SEC14	13..152	CDD:214706	75/138 (54%)
		SPEC	314..>468	CDD:238103	83/153 (54%)
		SPEC	563..778	CDD:238103	97/231 (42%)
		SPEC	782..997	CDD:238103	93/229 (41%)
		SPEC	897..1119	CDD:238103	125/240 (52%)
		SPEC	1125..1226	CDD:197544	60/100 (60%)
		RhoGEF	1287..1455	CDD:459876	105/168 (63%)
		PH1_Kalirin_Trio_like	1462..1584	CDD:270060	69/122 (57%)
		SH3_Kalirin_1	1643..1707	CDD:212786	28/77 (36%)
		RhoGEF	1945..2121	CDD:459876	75/175 (43%)
		PH2_Kalirin_Trio_p63RhoGEF	2130..2260	CDD:270061	42/137 (31%)
Kalrn	XP_011244253.1	PH1_Kalirin_Trio_like	1469..1591	CDD:270060	69/122 (57%)
		SH3_Kalirin_1	1657..1716	CDD:212786	28/63 (44%)
		SH3-RhoG_link	1714..1896	CDD:465196	53/219 (24%)
		RhoGEF	1939..2108	CDD:459876	75/175 (43%)
		PH2_Kalirin_Trio_p63RhoGEF	2113..2247	CDD:270061	43/141 (30%)
		SH3_Kalirin_2	2331..2387	CDD:212787
		I-set	2478..2572	CDD:400151
		Ig strand B	2495..2499	CDD:409353
		Ig strand C	2508..2512	CDD:409353
		Ig strand E	2538..2542	CDD:409353
		Ig strand F	2552..2557	CDD:409353
		Ig strand G	2565..2568	CDD:409353
		FN3	2576..2667	CDD:238020
		STKc_Kalirin_C	2697..2944	CDD:271017
		SEC14	47..185	CDD:214706	75/139 (54%)
		SPEC	198..418	CDD:238103	103/246 (42%)
		SPEC	319..544	CDD:238103	116/246 (47%)
		SPEC	545..774	CDD:238103	97/232 (42%)
		SPEC	899..1137	CDD:238103	125/240 (52%)
		SPEC	1143..1243	CDD:197544	60/100 (60%)
		RhoGEF	1292..1462	CDD:238091	106/169 (63%)