Protein Alignment trio and RhoGEF64C

DIOPT Version :9

• Sequence 1: NP_651960.2
• Gene: trio / 43974
• FlyBaseID: FBgn0024277
• Length: 2263
• Species: Drosophila melanogaster
• Sequence 2: NP_001261425.1
• Gene: RhoGEF64C / 38578
• FlyBaseID: FBgn0035574
• Length: 1984
• Species: Drosophila melanogaster
• Alignment Length: 1156
• Identity: 227/1156 - (19%)
• Similarity: 387/1156 - (33%)
• Gaps: 367/1156 - (31%)

  Fly   672 YTHEKDVTAWIDKLRTDVSTDETRLSQENLEGIERILQQYQRDQ---QESSVNV----CLTTISQ 729
            :|||      :.:|:...|.::..|..|........|.....:.   :::|:.:    |.:|::.
  Fly   962 FTHE------VHRLKISHSEEDADLGVELRRRPNLFLSSPGEEDAAPKKTSILISGDDCYSTMNL 1020

  Fly   730 GEALLQEMRSLEYADNTGSIAALEATLEKLNKQKVELEELWSARKFRADL---ILRLRYFERDAR 791
            .....|.:.......|:.|..::..::....:|:.||.:|.:.|..:..:   |:.:|.......
  Fly  1021 SANAAQPLYQSSVVVNSHSATSVCISVSSAEEQEQELNQLNTLRSQQMGMGIAIIPIRGASEVNL 1085

  Fly   792 LLSVQMEMWSEELQHADLSRDYQKAEQLIRMHNE-----SVTE---------------------- 829
            ..||.....:.....:..|....:...|||:..:     |.|.
  Fly  1086 QRSVSCISSTSSSSTSSSSSSSGRGRTLIRLDYDRERKGSATSSTPINTPPVISSTPATPPPTDL 1150

  Fly   830 -IQNATYEVVQQGQDLLQLFEN-AGFISMADATHTAQARIEYLLDFLRE----REIDLEEMSEAK 888
             |:||. .:|....:||::..| ...:.:....|....|..     .||    ::|..||:|...
  Fly  1151 PIENAN-PLVPHETELLKILRNPVEAVKLNLVPHVCGLRAP-----TRESGKSKKIQEEEVSGTP 1209

  Fly   889 ------RAKLEQAVQLCQFQNDANQVISWIRNG-EAMLVASFVTPNSLQEAEQLRKGHEQFQIAI 946
                  .|||.|...|.|           :..| |...||..: .||||..:|.|:|   ..::.
  Fly  1210 VKDPGFIAKLLQDPMLGQ-----------VAEGLETETVAELI-ENSLQRLQQARRG---IDMSG 1259

  Fly   947 EKTHTSAVQVKYRADALINANHYDPLSIDEISDDVTKKWQQLVTYAEERHKL----VTASINFYK 1007
            .|.|                            ||:.:    |::.:.:|.:|    .|..|:..|
  Fly  1260 TKDH----------------------------DDMNR----LISASLQRIRLERQPATPGISATK 1292

  Fly  1008 TAEQV-----CSVLDSLEREYKREDDWCDGGGGSDKAQAIVQLISKHQEQKEAFLKACTLAR--- 1064
            ..:::     .|..:|....:..|....|||.|.:::.......|:        |.|..:..
  Fly  1293 EEDRLSNRGSISSANSFASAHNYELFDFDGGAGGNESDCYQSCSSE--------LTASGILEEDS 1349

  Fly  1065 ----RTAETFLKYANRSQQCYQYKGNCEGHVKSKLDKLLTQENQVLDYWTLRKKSL--DQCQQFV 1123
                :..||.|..|.|: :.||.          .:|..|.:    :::.|...::|  |....:.
  Fly  1350 VSPIKDPETELDAATRA-KFYQL----------LVDATLAE----IEHSTHMDQTLGTDSEHHYE 1399

  Fly  1124 LFERSAKQAIEWIHNT-----------GEAYL---SSRSNLVGISK---------EETEGLLKEH 1165
            ....|.....|.||..           .:|.|   ::||...|.||         .:..||.:|.
  Fly  1400 SIRHSGDPIYEEIHEVPPPLPLSAPPLNDAELEKKAARSIFEGASKYDILSYLVDAKERGLAREE 1464

  Fly  1166 N-EFRSTAKETRERVKLLIQLADSLVEKGHAHASDIKQWVASVDQRYKDF-SNRMDS-------- 1220
            : ::.:..|...|      :.||:|        ||:.:..|...:|.:|. .:.|.|
  Fly  1465 SFDYGNNPKIIEE------EAADTL--------SDLDKRQAEERERDRDSGQSSMSSLSPPPHNF 1515

  Fly  1221 YCEQLEKSLGMSQQQLLLGSDGNSSISSSSGDRHSDPTLEAKLNSSNKENKEINEE--------- 1276
            .|....||.|...::...|....:|.:|.|..:.:...|....:.......::.:.
  Fly  1516 ICGGAGKSSGSDVERQDSGVGSETSKTSRSKYQATPTVLHLCEDCDGAVEMQVGDNGILYAPLVC 1580

  Fly  1277 ---KRKSARRKEFIMAELMQTERAYVNDLATCIKCFLEEFRAGKSVPSALIGQEDV--IFGNIKE 1336
               .:|...||| |:.|:::||..|..||    :..|||| ....:.:.|:.||.:  ||.|.::
  Fly  1581 RKCGKKRVERKE-IITEIVETEEKYGRDL----QIILEEF-CHPMLVAGLLTQEQLSAIFLNTED 1639

  Fly  1337 IHHFHQKIFLRELEKYETMPE--------------------DVGHCFVTWASKFDMYVHYCKNKP 1381
            :           ||..:|:.|                    ::|..|:.:......:..||..:.
  Fly  1640 L-----------LENNQTLAERMRDALDMSLEQGDDDLLTVNIGRIFLDFTQMLHAFESYCVRQA 1693

  Fly  1382 TSNNLLVQHGGS------FFEELQ------RRLEVDHPLPAYLIKPVQRITKYQLLLKDLLSCCE 1434
            .::.||......      |.:..|      ||:.    |.::|:.||||:|||.|||..|.. ..
  Fly  1694 GASLLLANLEKEKELLRIFLKVSQMENAVLRRMN----LNSFLMVPVQRVTKYPLLLARLYK-VT 1753

  Fly  1435 ESHGEIKEGLEVMLNVPKKANDAMHLSLL--ENCDVSVDKLGEVVLQDAFQAWDTKQIIRKGRER 1497
            .||   .||.|::....:|..  :||:.:  |..||..            :.|  ::|......|
  Fly  1754 PSH---LEGRELLKQAQEKIE--LHLNHINQEAKDVPT------------KLW--RRISSSSPNR 1799

  Fly  1498 RVFLFELYLLFAKEVKESNVVKYQFKSKLMTTDMGITEHIEGDETKFAVWTGRSPMLSDCRIVLK 1562
            |.         :.|:...|:     |.:.|..|:....|   ||.:||: .||          |.
  Fly  1800 RA---------SCEIDMINI-----KLRKMAIDVLEWNH---DEVRFAM-EGR----------LL 1836

  Fly  1563 ATSLETKQIWVKKLREVMQETCFSGTSLTLPKSPAKHSGSSQRSSRDLDEQLTENDH-------- 1619
            .|. .|...| ||.|.: :.|..:...:|..|..|.:     |:.:.:.|:|....|
  Fly  1837 YTQ-PTDSNW-KKARTI-KLTPVNALLVTNGKPSANY-----RAEKAMSEKLNFPKHTGIREASL 1893

  Fly  1620 -------------------DRCSLASFGSGNTTDSDNKLGNQEATWVVADY 1651
                               |||.:.|                ||.|  .||
  Fly  1894 LLVKEKCGRYTLIREPLYLDRCVVCS----------------EADW--DDY 1926

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
trio	NP_651960.2	SEC14	13..152	CDD:214706
		SPEC	314..>468	CDD:238103
		SPEC	563..778	CDD:238103	19/112 (17%)
		SPEC	782..997	CDD:238103	47/254 (19%)
		SPEC	897..1119	CDD:238103	45/240 (19%)
		SPEC	1125..1226	CDD:197544	27/133 (20%)
		RhoGEF	1287..1456	CDD:279015	50/202 (25%)
		PH1_Kalirin_Trio_like	1462..1584	CDD:270060	26/123 (21%)
		PH	1495..1580	CDD:278594	21/84 (25%)
		SH3_Kalirin_1	1643..1707	CDD:212786	5/9 (56%)
		RhoGEF	1945..2122	CDD:279015
		PH2_Kalirin_Trio_p63RhoGEF	2130..2260	CDD:270061
RhoGEF64C	NP_001261425.1	RhoGEF	1593..1779	CDD:279015	52/211 (25%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3601
SonicParanoid	0	0.000	Not matched by this tool.
	1	1.030