Protein Alignment trio and triob

DIOPT Version :10

• Sequence 1: NP_651960.2
• Gene: trio / 43974
• FlyBaseID: FBgn0024277
• Length: 2263
• Species: Drosophila melanogaster
• Sequence 2: XP_073781895.1
• Gene: triob / 368847
• ZFINID: ZDB-GENE-030616-399
• Length: 3023
• Species: Danio rerio
• Alignment Length: 2361
• Identity: 1024/2361 - (43%)
• Similarity: 1460/2361 - (61%)
• Gaps: 249/2361 - (10%)

  Fly     2 DGQRARDLLTLLQERVVFLTGGRDRRGGPLLCFPATPRRDRLKPEDLRRLLSYLISIPSDAAKNL 66
            :..:|.::|.:|:|:|.||:|||||||||:|.|||....||::.||||||::||..|||:.....
Zfish    45 EDMKAIEVLPILKEKVAFLSGGRDRRGGPVLTFPARSNHDRIRQEDLRRLIAYLAGIPSEEVSRH 109

  Fly    67 GFTVIIDMRGNGNCSTNVKTILKVLQEHFSANIHNVVIIKPDNFWQKQRASISSHKYKFETTTVS 131
            |||||:||||:.  ..::|.:||:|||.|.:.||..:||||||||||||.:..|.|::|||..||
Zfish   110 GFTVIVDMRGSK--WDSIKPLLKILQESFPSCIHVALIIKPDNFWQKQRTNFGSSKFEFETIMVS 172

  Fly   132 IESLNKIVESHQLTGDFEGQQLYDHQQWTDARLAIEDFFWQAGDMADRIDDLQEDLNRNDFAEDV 196
            :|.|.|:|:..|||.||||...|||::|.:.|:|.|||...|..:..|:::||:.|::.:...|:
Zfish   173 LEGLTKVVDPSQLTPDFEGSLDYDHEEWIEVRVAFEDFTSNAARILARLEELQDMLSQRELPSDL 237

  Fly   197 PLARHAIDHHNEMRKKITKLPIEDLDMQGKKLLAKINAMAPPGGQAPTNSGGPDMDSGPSSAGQQ 261
            ..:|.|::.|..::||:||.|:|:||.:|:.||.:|..         .:.|..|:.
Zfish   238 DGSRRAMEEHASLKKKVTKAPVEELDTEGQHLLQRIQC---------ADKGRGDIQ--------- 284

  Fly   262 SQPSQQSRSVGGNPDMSAAVNKALRQIELIHTGQEKLLMLWQHKKVKLDQCFQWRLFEQDCEKMF 326
                      |..|.:.|.::|       :|..::.|...|..:|:|||||||.|||:||.||||
Zfish   285 ----------GLAPKVQALLDK-------LHGTRQHLHQAWHLRKLKLDQCFQLRLFQQDAEKMF 332

  Fly   327 DWILHNRDVFQMSYVEIGHNYSVAKSLQDEHQKFAVASMNVSVNIDRILAVAARLIESQHYAAQH 391
            |||:||:.:|..:|.|||.|:.....||.:|..||:..|||.|||.||::|..||:||.|||.|.
Zfish   333 DWIVHNKGLFLTTYTEIGANHQHVMELQTQHNHFAMNCMNVYVNISRIMSVGNRLLESGHYATQQ 397

  Fly   392 IKTLAQRLDRTWKDFAAGLDERTAVLQLSVLFHHKAEQYCNSVASWAAACQASQPLPSDIQSLET 456
            |:.::.:|::.||.|||.||||:.:|::|..||.|.:||.:::.||..||...: |||::|.||.
Zfish   398 IQQISGQLEQEWKAFAAALDERSTLLEMSANFHQKTDQYMSNMDSWCKACGEGE-LPSELQDLED 461

  Fly   457 AIRTHQSLYEAMCQAYTEVHSTSKKLLYQLDHLVQVCNQPPPPGVPDHRKNSNNGSFNKYERQNP 521
            .|..||.|||.:..||:||....|.||.:|       .:|..||..|...:|
Zfish   462 TIHHHQGLYEHITAAYSEVSQDGKALLDKL-------QRPLTPGSADSLTSS------------- 506

  Fly   522 AADYSEGASHVLAVIHQILSHHRTLEAKWHQERLRLHQTLALRLFQEDVKQVLDWLKNHGEVFLR 586
             |:||:...|||.:||::|.|.|.||..|...:|||||.|.|.:||:||:|||||::||||.||.
Zfish   507 -ANYSKAVHHVLDIIHEVLHHQRQLENIWQHRKLRLHQRLQLCVFQQDVQQVLDWIENHGEAFLS 570

  Fly   587 KNTGIGRNFHKARVYQTSHDNFENVAQNTYSNAQKLLAAADELARSGEADPNEIYSVARELELQV 651
            |:||:|::.|:||..|..|::||.||||||:||.|||.||::||::||.||.|||..|.:||.::
Zfish   571 KHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRI 635

  Fly   652 GSFAERVEQRRRRLDMAVIFYTHEKDVTAWIDKLRTDVSTDETRLSQENLEGIERILQQYQRDQQ 716
            ..|..|||||:..|||:|.|:||.|::..|:::|:.::..|   :..|::|.::.:::::.: ||
Zfish   636 QDFVRRVEQRKVLLDMSVAFHTHSKELWTWLEELQKELLDD---VYAESVEAVQELIKRFGQ-QQ 696

  Fly   717 ESSVNVCLTTISQGEALLQEMRSLEYADN----TGSIAALEATLEKLNKQKVELEELWSARKFRA 777
            ::::.|.:..|.:||.|:|::|....:.|    ..|:|.:|:.|::|::.:.::|||:..||.:.
Zfish   697 QTTLQVTVNVIKEGEDLIQQLRDSAISSNKTPHNSSMAHIESVLQQLDEAQAQMEELFQERKIKL 761

  Fly   778 DLILRLRYFERDARLLSVQMEMWSEELQHA----DLSRDYQKAEQLIRMHNESVTEIQNATYEVV 838
            :|.|:||.|||||..:...:|.|:|||...    | :.|...|||.::.|.:....:.|.|:.|:
Zfish   762 ELFLQLRIFERDAIDVISDLESWNEELSQQMNEFD-TEDLTLAEQRLQHHADKALTMNNLTFHVI 825

  Fly   839 QQGQDLLQL---FENAGFISMADATHTAQARIEYLLDFLREREIDLEEMSEAKRAKLEQAVQLCQ 900
            .|||:|||.   .:.:|...:.|.......|::.|||||.|::.:|:..:|..|..|||.|||..
Zfish   826 HQGQELLQYVTEVQASGVELLCDRDVDMATRVQDLLDFLHEKQQELDAAAEQHRRHLEQCVQLRH 890

  Fly   901 FQNDANQVISWIRNGEAMLVASFVTPNSLQEAEQLRKGHEQFQIAIEKTHTSAVQVKYRADALIN 965
            .|.:..||:.||||||:||.|..:|.:||||||||::.|||||.||||||.||:||:.:|:||:.
Zfish   891 LQAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQREHEQFQHAIEKTHQSALQVQQKAEALLQ 955

  Fly   966 ANHYDPLSIDEISDDVTKKWQQLVTYAEERHKLVTASINFYKTAEQVCSVLDSLEREYKREDDWC 1030
            |||||...|.:.::.|...||||:...|:|.|||.||:.||||:|||||||:|||:|||||:|||
Zfish   956 ANHYDMDMIRDCAEKVASHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWC 1020

  Fly  1031 DGGGGSDK------AQAIVQLISKHQEQKEAFLKACTLARRTAETFLKYANRSQQCYQYKGNCEG 1089
               ||:||      :..:..::|||.|||||||||||||||.|:.||||.:|:..      |..|
Zfish  1021 ---GGTDKLGPNSESDHVTPMVSKHLEQKEAFLKACTLARRNADVFLKYLHRNSV------NMPG 1076

  Fly  1090 ---HVKSK-------LDKLLTQENQVLDYWTLRKKSLDQCQQFVLFERSAKQAIEWIHNTGEAYL 1144
               |||:.       |::||.:||:||.:||:||:.||||||:|:||||||||:||||:|||.||
Zfish  1077 MLAHVKAPEQQVKNILNELLQRENRVLHFWTMRKRRLDQCQQYVVFERSAKQALEWIHDTGEFYL 1141

  Fly  1145 SSRSNLVGISKEETEGLLKEHNEFRSTAKETRERVKLLIQLADSLVEKGHAHASDIKQWVASVDQ 1209
            |:.:: .|.:...|:.|||||.||:.|||:|:|||||||||||...||||||||:|::||||||:
Zfish  1142 STHTS-TGSTIHHTQELLKEHEEFQITAKQTKERVKLLIQLADGFCEKGHAHASEIQKWVASVDK 1205

  Fly  1210 RYKDFSNRMDSYCEQLEKSLGMSQQQLLLGSDGNSSISSSSGDRHSDPTLEAKLNSSNKENKEIN 1274
            ||:|||.|||.|...|||:||:|........|....|..:|.     |..|.||..   .|.|:|
Zfish  1206 RYRDFSLRMDKYRSCLEKALGLSTDSNKASKDLQLDIIPASA-----PGAEVKLRD---VNHELN 1262

  Fly  1275 EEKRKSARRKEFIMAELMQTERAYVNDLATCIKCFLEEFRAG-KSVPSALIGQEDVIFGNIKEIH 1338
            |||||||||||||||||:|||:|||.||..|:..:|.|..:| :.:|..::.:|.:||||:::::
Zfish  1263 EEKRKSARRKEFIMAELIQTEKAYVRDLRECMDTYLWEMSSGVEEIPPGIVNKEHIIFGNMQDLY 1327

  Fly  1339 HFHQKIFLRELEKYETMPEDVGHCFVTWASKFDMYVHYCKNKPTSNNLLVQHGGSFFEELQRRLE 1403
            .||..|||:||||||.:||||||||||||.||.|||:||||||.|..|:::|.|.:|:|:|:|..
Zfish  1328 EFHHNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVNYCKNKPDSTQLILEHAGPYFDEIQQRHR 1392

  Fly  1404 VDHPLPAYLIKPVQRITKYQLLLKDLLSCCEESHGEIKEGLEVMLNVPKKANDAMHLSLLENCDV 1468
            :.:.:.:|||||||||||||||||:||:||||..||||:||||||:|||:||||||||:||..|.
Zfish  1393 LANSISSYLIKPVQRITKYQLLLKELLTCCEEGKGEIKDGLEVMLSVPKRANDAMHLSMLEGFDE 1457

  Fly  1469 SVDKLGEVVLQDAFQAWDTKQIIRKGRERRVFLFELYLLFAKEVKESN-VVKYQFKSKLMTTDMG 1532
            :::..||::||::||.||.|.:|||||||.:||||:.|:|:|:||:|| ..||.:||||||:::|
Zfish  1458 NIESQGELILQESFQVWDPKTLIRKGRERHLFLFEMSLIFSKDVKDSNGRSKYLYKSKLMTSELG 1522

  Fly  1533 ITEHIEGDETKFAVWTGRSPMLSDCRIVLKATSLETKQIWVKKLREVMQETCFS-----GTSLTL 1592
            :|||:|||..|||:|.||:| .||.:|||||:.:|.||.|:|.:|||:||....     ...:.:
Zfish  1523 VTEHVEGDPCKFALWVGRTP-TSDNKIVLKASCIENKQDWIKHVREVIQERTIHLRGALKEPIHI 1586

  Fly  1593 PKSPA-KHSGSSQRSSRDLDEQ-LTENDHDRCSLASFGSGNTTDSDNKLGNQEATWVVADYIATS 1655
            ||:.| ||.| .:|...|||.| ...:..|..|:||..|.||.|||...|..|.|.|:.|::|::
Zfish  1587 PKATATKHKG-KRRDGEDLDSQGDASSQPDTISIASRTSQNTLDSDKLSGGSELTVVIHDFMASN 1650

  Fly  1656 GSNELSVSKGQQVEIVEPPTAGEPDFCLVRLNPQHDDAAVQEGLVPVSVL--------KPPPG-- 1710
            |: ||:|.:||.||::|.| ..:||:||||..   |.:..||||||.::|        ....|
Zfish  1651 GA-ELTVRRGQTVELLERP-QDKPDWCLVRTT---DRSPAQEGLVPSAMLCIDHSRSSMEMEGIF 1710

  Fly  1711 SHKHGSGTAANAASSAGS---QKSDMQDQSNRSKADALSSSTKRRGFSGRNWL--PLMN------ 1764
            :||.....::|....:||   |.|.:|.          |...||.|.:.|.||  |:..
Zfish  1711 NHKDTLSVSSNEGGISGSATLQPSHLQS----------SPGPKRPGNTLRKWLTSPVRRLSSGKA 1765

  Fly  1765 ----RKGTDKPPSSKPLVK-------KPSEKNLRTPQKHAEELAEQQNQQPGASPATVLPSSQFP 1818
                :|...|...|:.:.|       |.|:.:..|||   :|..|::.:..|.|..|:..||
Zfish  1766 DGHVKKLAHKHKKSRDVRKNADGGSQKDSDDSAATPQ---DETIEERMRNEGLSSGTLSKSS--- 1824

  Fly  1819 SSTQHPGGAGAGDYEPDEEVGLELPPPMKPIQEPHLIANGPPAFAKDAKESSGNMASPGKMDGNP 1883
            ||.....|...|:..||   .:.||||| .||:..|:       .:|::|...     .::.|.|
Zfish  1825 SSGMQSCGEEEGEEGPD---SVPLPPPM-AIQQHSLL-------HQDSQEDKA-----ARLSGRP 1873

  Fly  1884 -----------LSEIEEIVKT--TTEQHESNSRVDGGGGVENASTNGHGRSHDNNDESHTAVSD- 1934
                       :|.|||:||:  ..|...|:..|:     ...||:......||:..|.::..|
Zfish  1874 SSSETPSAAELVSAIEELVKSKMNLEDRPSSLPVE-----HRDSTSPSCNPSDNSLLSSSSPIDE 1933

  Fly  1935 ----KAAALKKRQCVFAELMSTEEAYVQDLHEIVNGYMTEINNTNSDIPMPEDLKGGKMKLVFNN 1995
                |...||:|..|..||:.||..||:||..:|.|||:.:....    :|:|:| ||.|:||.|
Zfish  1934 MDERKTGFLKRRHYVLLELVETERDYVRDLGAVVEGYMSRMKEEG----VPDDMK-GKDKIVFGN 1993

  Fly  1996 IKDIYEWHRDFFLRALRNCQKSPADLGPLIKRSATKFALYYTYCSNKPLSEYIVSAHYQ-YFDSI 2059
            |..|::||:||||..|..|.:.|..|.||..|...:..:|..||.|||.||:|||.:.. ||:.:
Zfish  1994 IHQIFDWHKDFFLAELEKCLEDPDRLAPLFVRQERRLHMYIVYCQNKPKSEHIVSEYIDTYFEDL 2058

  Fly  2060 RQKLGHRLDLSNLIIKPVQRITKYELLIKEIIKATEGAGLYKEVPMLQEAYQQMKVVVKTVNDMM 2124
            :|:|||||.:::|:|||||||.||:||:|:::|.|:.|||  :...|::|.:.|.||.|..||||
Zfish  2059 KQRLGHRLQITDLLIKPVQRIMKYQLLLKDLLKFTKKAGL--DTVDLEKAVEVMCVVPKRCNDMM 2121

  Fly  2125 VVLRSLQDFDGEITAQGSLLMQGPMNCVVD----AAQKHRELQVFLFQQIIIFADIEKTKNQYAS 2185
            .|.| ||.|||:|.|||.||:|... .|.|    ...:.:|.::|||:||:||::....|..::.
Zfish  2122 NVGR-LQGFDGKIVAQGRLLLQDTF-MVSDQDGGLLSRLKERRIFLFEQIVIFSEPLDKKKGFSM 2184

  Fly  2186 PIFKYRSHIQLNHMQMKELGD---CRFQIRSTDPKIPEMTIICQAASQENYA----------GWR 2237
            |.:.:::.|:::.:.::|..|   |:|.:.|..          .|.|.|:|.          .|.
Zfish  2185 PGYLFKNSIKVSWLGLEESPDNDPCKFILTSRS----------SAGSLEHYVLHSSNRVVCQTWI 2239

  Fly  2238 DMLNKILQQQNDLIFMLSNPL 2258
            ..::.||:.|.:.:..|::|:
Zfish  2240 HQISSILENQRNFLNALTSPI 2260

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
trio	NP_651960.2	SEC14	13..152	CDD:214706	79/138 (57%)
		SPEC	314..>468	CDD:238103	77/153 (50%)
		SPEC	563..778	CDD:238103	91/218 (42%)
		SPEC	782..997	CDD:238103	97/221 (44%)
		SPEC	897..1119	CDD:238103	127/237 (54%)
		SPEC	1125..1226	CDD:197544	66/100 (66%)
		RhoGEF	1287..1455	CDD:459876	101/168 (60%)
		PH1_Kalirin_Trio_like	1462..1584	CDD:270060	69/122 (57%)
		SH3_Kalirin_1	1643..1707	CDD:212786	29/71 (41%)
		RhoGEF	1945..2121	CDD:459876	80/176 (45%)
		PH2_Kalirin_Trio_p63RhoGEF	2130..2260	CDD:270061	40/146 (27%)
triob	XP_073781895.1	None