Protein Alignment trio and plekhg4

DIOPT Version :10

• Sequence 1: NP_651960.2
• Gene: trio / 43974
• FlyBaseID: FBgn0024277
• Length: 2263
• Species: Drosophila melanogaster
• Sequence 2: XP_073764743.1
• Gene: plekhg4 / 326099
• ZFINID: ZDB-GENE-030131-4824
• Length: 2679
• Species: Danio rerio
• Alignment Length: 2051
• Identity: 423/2051 - (20%)
• Similarity: 721/2051 - (35%)
• Gaps: 576/2051 - (28%)

  Fly    44 KPEDLRRLLSYLISIPSDAAKNLGFTVIIDMRGNGNCSTNVKTILKVLQEHFSANIHNVVIIKPD 108
            |...:.:|::.:.|.|....|::..|.:         .:.:...|:..||  |.:::|.:..:|.
Zfish   786 KQVSISQLVNEIFSTPQPDNKSVSTTQL---------DSKLVNTLQDKQE--SKSVNNSIDNEPV 839

  Fly   109 NFWQKQRASISSHKYKFETTTVSIESL---------------------NKIVESHQLTGDFEGQQ 152
            ...|.....:  .:.:|::..||...|                     ||.|.:.||.|:.
Zfish   840 KTSQPDTEPL--RRSQFDSNPVSNSQLDGKQFSISQLVSEIFSTTQPDNKSVSTTQLDGNI---- 898

  Fly   153 LYDHQQWTDARLAIEDFFWQAGDMADRIDDLQEDLNRNDFA--------EDVPLARHAID---HH 206
                                  |...::|..||.:::.|..        |.|..::..::   |.
Zfish   899 ----------------------DRTSQLDSKQESVSQTDSKPVSISPDNEPVKTSQPQLESTLHP 941

  Fly   207 NEMRKKITKLPIEDL-----DMQGKKLLAKINAMAPPGGQAPTNSGGPDMDSGPSSAGQQSQ--- 263
            |..:..:::|..|.:     |::...:....:..|.|.|.|.......::|:|..|..|...
Zfish   942 NSKQASMSQLVNEHIQTSQPDLKPVSIAQLYSEDAKPDGYADVKPITTELDNGLVSTSQPDNQPV 1006

  Fly   264 --PSQQSRSVG-----GNP-DMSAAVNKAL--------------RQIELIHTGQEKLLMLWQHKK 306
              |..:..:|.     ||| |.|..||..|              ..|.:|:|.|.:..||
Zfish  1007 DTPQAECTTVNITLPEGNPNDTSYIVNLPLSTSQPNSKTVHVEQADINVINTSQPETEML----- 1066

  Fly   307 VKLDQCFQWRLFEQDCEKMFDWILHNRDVFQMSYVEIGHNYSVAKSLQDEHQKFAVASMNVSVNI 371
                .|    :.|::          |.:....:|:|:        .:.|.:.:....|..:.||.
Zfish  1067 ----SC----VLERE----------NSEQVSPAYIEL--------IVSDPYAEVRCESKELRVNC 1105

  Fly   372 DRILAVAARLIE----SQHYAAQHIKTLAQRLDRTWKDFAAGLDERTAVLQLSVLFHHKAEQYCN 432
            |         :|    ||:..|...:|:|:                      ||...::.:|..|
Zfish  1106 D---------LEGSCGSQNNRASLAETVAE----------------------SVSSQNQIDQTGN 1139

  Fly   433 SVASWAAACQASQPL--PSD-IQSLETAIRTHQSLYEAMCQAYTEVHSTSKKL------------ 482
            |:      .|..:|:  ||: :..|.|....||:...:.       |:|::.:
Zfish  1140 SL------YQMHKPIHTPSEQMHDLPTIKEDHQTFSSSS-------HNTNESVGDLSPIAEEDGG 1191

  Fly   483 -----LYQLDH--LVQVCNQPPPPGV------------PDHRKNSNNGSF-NKYER--QNPA--- 522
                 ||. :|  ||.|...|....|            |:.|.|...... .|.||  ||.|
Zfish  1192 SVTAALYS-EHVALVSVTTVPGENPVTLISQGQVGTESPEDRINQTEVELQTKEERAVQNVAGMP 1255

  Fly   523 ----ADYSEGASHVLAVIHQILSHHRTLEAKWHQERLRLHQTLALRLFQEDVKQVLDWLKNHGEV 583
                |:..|..|...|   :|.:....:.|...||.:...||..:....:...||.:.:.:..|.
Zfish  1256 PCKGAEMVEKKSSSEA---EIKTDENDVVAVCRQEEVETSQTGLVDATPKSDVQVQEEVSSPVET 1317

  Fly   584 FLRKNT-----GIGRNFHKARVYQTSH----DNFENVAQNTYSNAQKLLAAADELARSGEADPNE 639
            ..:|.|     |:.....||:..:...    .|.||..|:..|  :.|..|:|....:|.....:
Zfish  1318 DEKKQTSVQEKGVEEEEVKAKTVREQEAIVTSNSENRVQSACS--EVLDPASDVPNTNGHMHAQD 1380

  Fly   640 IYSVARELELQV-----GSFAERVEQ--------------RRRRLDMAVIFYTHEKDVTAWIDKL 685
              :...||:.|:     |..||...|              .:::.||...|  |:::..|...|:
Zfish  1381 --TPTTELKGQITKEEDGKGAEEKRQEDEIASSVNGSQKEHQQQADMDSSF--HQQEEQACSQKV 1441

  Fly   686 RTDVSTDETRLSQENLEGIERILQQ-YQRDQQESS----VN--------VCLTTI--SQGEALLQ 735
            .:.|.....:...|:...:...... |.|.|...|    :|        :|||..  ..|.||:.
Zfish  1442 DSTVCHLSAKTESEDPSAVPSPQPPVYHRAQPVKSKAHDINPDVLSSGVLCLTGTRDKSGHALVT 1506

  Fly   736 -EMRSLEYAD---NTGSIAALE----ATLEKLNKQKVELEELWSARK-------FRADLILR--- 782
             .||:..:.:   |:|.:..:.    :||.| ....:.|..|..||:       |:|..||:
Zfish  1507 VTMRNTVWLNPNCNSGELVRIMVYFCSTLRK-EVSALGLTVLVDARRCSPVPALFKAFNILQEAM 1570

  Fly   783 -------LRYFERDARL-----LSVQMEMWS---EELQHADLSRDYQKAEQLIRMHNESVTEIQN 832
                   |...:||..|     .|:|:|:.:   ...:|.|:|:...:.|......:.|....:.
Zfish  1571 PGCIHTVLLLADRDLALRMEKTSSIQVELLTSLKSLYKHVDVSQLPTEFEGTFPYAHSSWVCFRM 1635

  Fly   833 ATYEVVQQGQDLLQLFENAGFISMADATHTAQARIEYLLDFLREREIDLEEMSEAKRAKL----- 892
            ...::....:|.:.|.:|.  ||..::|.......|..|...:.||:....:.:::..:|
Zfish  1636 RLEQLTSHCEDAVSLLQNT--ISKLESTVLPPTAEEAQLLLCKYRELMRTVLEDSRLVRLQLEGG 1698

  Fly   893 ---------EQAVQLCQFQNDANQVISWIRNGEAMLVASFVTPNS--------LQEAEQLRKGHE 940
                     |.:|.|.:...||.:....:.|....||...|..::        :.|...|.:|.:
Zfish  1699 AALSRLRKEETSVSLTEDYRDAIESAGRLYNQVDELVHRLVMLSNKCTQELEFIMEFNSLEEGFK 1763

  Fly   941 QFQIAIEKTHTSAVQVKYRADALINANHYDPLSIDEISDDVTKKWQQLVTYAEERHKLVTASINF 1005
            :....||:...|.:|                 ::.|:.|.:           |:.|...:....|
Zfish  1764 EVSQWIEEVGESRLQ-----------------TLSELEDSL-----------EQLHHKQSIFREF 1800

  Fly  1006 YKTAEQVC----SVLDSLER------------EYKREDDWCDGGGGSDKAQAIVQLISKHQEQKE 1054
            |..|.:.|    ::|..||:            |.|....|......|.:.:.....|.|..:..|
Zfish  1801 YTAAYEHCKSGEALLKRLEKWEDVSSAELQVYEVKVRSFWVHLRDFSQRVEDTKDKIDKTVQLYE 1865

  Fly  1055 AFLKACTLA----RRTAETFLKYANRSQQC---------------------YQYKGNCEGHVKSK 1094
            .|.||...|    |..|...::....|::|                     :|...:..|.:||:
Zfish  1866 FFDKAYEWALEGMRHLACVSMEDCGMSEKCQSVITCLETYRSQHPPIPDAHFQEMKDLAGELKSE 1930

  Fly  1095 LDKLLTQENQVLDYWTLRKKSLDQCQQF-VLFERSAKQAIEWIHNTGEAYLSS------------ 1146
                     |.|..|   |.:..:||:. .:||:..:.|:....::....:.|
Zfish  1931 ---------QGLKQW---KFAWSKCQETKQMFEKKLEMALRSRRDSDSKSVRSDSSRRNSDVSTK 1983

  Fly  1147 ---RSNLVGISKEETEG------LLKEHNEFRSTAK-------ETRERVK--------LLIQLAD 1187
               ||:.:..|..:..|      .|..|:...||..       :.|:.|:        :..::..
Zfish  1984 HQERSSSISFSCRKAFGGAWGRDRLSSHSSTSSTPSSPSGIRLDARDTVRTPTGSPYSIEHRIPH 2048

  Fly  1188 SLVEKGH--------------AHASDIKQWVAS-----VDQRYKDFSNRMDSYCEQLEKSLGMSQ 1233
            |...:.|              .||..:.....|     .::|....:...|:...:.....|..|
Zfish  2049 STPVRSHRLTRSISTDESPQSPHAEGLACTSPSGTSVQTNRRVLRKTQSFDTAGSEPVSRYGTCQ 2113

  Fly  1234 QQLLLGSD----GNSSI--------SSSSGDR---------HSDPTLEAKLNSSNKENKEINEEK 1277
            :.|   |:    ||:.:        |:...||         |...:::|..:.|..      .|.
Zfish  2114 RTL---SEPARRGNTGVFIKGLEVSSTEVIDRPYSPRLPPIHGWSSIDAHRSGSPA------PET 2169

  Fly  1278 RKSARRKEFIMAELMQTERAYVNDLATCIKCFLEEFRAGKSVPSALIGQEDVIFGNIKEIHHFHQ 1342
            |....:...|:.|::.|||.||..|...|..:..|.... .:|..|.|:..|||||::::..||.
Zfish  2170 RSKGSKLRHIVDEMVTTEREYVRSLRYIIDNYFPEMERA-DLPQDLRGKRSVIFGNLEKLVDFHS 2233

  Fly  1343 KIFLRELEKYETMPEDVGHCFVTWASKFDMYVHYCKNKPTSNNLLVQHGGSFFEELQRRLEVDHP 1407
            :.||:|||.....|..|.|||:....:|.:|..|.||||.|:.||..||.:||:..|..|.....
Zfish  2234 QYFLKELESCCNHPLRVSHCFLRHQDQFSLYALYSKNKPKSDTLLASHGNNFFKHKQMELGDKMD 2298

  Fly  1408 LPAYLIKPVQRITKYQLLLKDLLSCCEESHGE----IKEGLEVMLNVPKKANDAMHLSLLENCDV 1468
            |.:||:||:||::||.||||||:....|...:    ::...|::....:..||.:.:..:.:|||
Zfish  2299 LASYLLKPIQRMSKYALLLKDLIKEVSEVQEQELTYLRAAAEMVKFQLRHGNDLLAMDAIRDCDV 2363

  Fly  1469 SVDKLGEVVLQDAFQAWDTKQIIRKGRERRVFLFELYLLFAKEVK-ESNVVKYQFKSKLMTTDMG 1532
            ::.:.|::|.||.|    |....||..:|.|||||..:||:|..: |..:..|.:|....|.|:|
Zfish  2364 NLKEQGQLVRQDEF----TISYGRKKCQRHVFLFEDLVLFSKPKRIEGGLDVYIYKHSFKTADVG 2424

  Fly  1533 ITEHIEGDETKFAVWTGRSPMLSDCRIVLKATSLETKQIWVKKLREVMQETCFSGTSLTLPKSPA 1597
            :||....:..:|.:|..|....:. ..:|::::.:.|..|          ||
Zfish  2425 MTETSGENSLRFEIWFRRRTSKNQ-TYILQSSTADIKHAW----------TC------------- 2465

  Fly  1598 KHSGSSQRSSRDLDEQLTENDHDRC-SLASFGSGNTTDSDNKLGNQEATWVVADYI------ATS 1655
                   ..:|.|.:|.|.|...|. .:.|.|.||....|.|..:........|||      .|.
Zfish  2466 -------DIARILWQQATRNKEIRMQEMVSMGVGNKPFLDIKPSDAAINDRAIDYIMKGRGARTR 2523

  Fly  1656 GSNELSV---SKGQQVEIVEPPTAGEP-------DFCLVRLN-PQHDDAAVQEGLVPVSVLKP-- 1707
            .|..:|:   |...:...|..|.:|.|       ...|..|| ..:...::..|..|  ::.|
Zfish  2524 ASIAVSLFDHSNPFKRAAVNTPVSGTPVSGGPSSSTLLGPLNLHMYSSQSLLAGERP--LIGPCI 2586

  Fly  1708 -----------PPGSHKHGSGTAANAASSAGSQKSD 1732
                       .|......||::::..|.:||..||
Zfish  2587 EEDELEHETSSQPSMTTESSGSSSHCLSGSGSSGSD 2622

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
trio	NP_651960.2	SEC14	13..152	CDD:214706	23/128 (18%)
		SPEC	314..>468	CDD:238103	28/160 (18%)
		SPEC	563..778	CDD:238103	55/272 (20%)
		SPEC	782..997	CDD:238103	42/254 (17%)
		SPEC	897..1119	CDD:238103	48/270 (18%)
		SPEC	1125..1226	CDD:197544	21/155 (14%)
		RhoGEF	1287..1455	CDD:459876	63/171 (37%)
		PH1_Kalirin_Trio_like	1462..1584	CDD:270060	34/122 (28%)
		SH3_Kalirin_1	1643..1707	CDD:212786	16/80 (20%)
		RhoGEF	1945..2121	CDD:459876
		PH2_Kalirin_Trio_p63RhoGEF	2130..2260	CDD:270061
plekhg4	XP_073764743.1	None