DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment trio and Arhgef25

DIOPT Version :9

Sequence 1:	NP_651960.2	Gene:	trio / 43974	FlyBaseID:	FBgn0024277	Length:	2263	Species:	Drosophila melanogaster
Sequence 2:	XP_006241516.1	Gene:	Arhgef25 / 314904	RGDID:	735210	Length:	613	Species:	Rattus norvegicus

Alignment Length:	616	Identity:	171/616 - (27%)
Similarity:	269/616 - (43%)	Gaps:	125/616 - (20%)

- Green bases have known domain annotations that are detailed below.

  Fly  1674 PTAGEPDFCLVRLNPQHDDAAVQEGLVPVSVLKPPPGSHKHGSGTAANAASSAGSQKSDM----Q 1734
            ||.|..|..|          .|..|::....:   ||.    .|.........||.|::.    :
  Rat     7 PTPGRTDRIL----------GVMGGMLRACAV---PGQ----EGPQERDPLGPGSTKTESECTEE 54

  Fly  1735 DQSNRSKADALSSSTKRRGFSGRNWLPLMNRKGTDKPPSSKPLVKKPSEKNLRTPQKHAEELAEQ 1799
            ||:...:.:.|:            |                            .||..:..:|..
  Rat    55 DQTGEREREVLA------------W----------------------------APQPESYSIAGS 79

  Fly  1800 QNQQPGASPATVLPSSQFPSS--TQHPGGAGAGDYEPDEEVGLELPPPMKPI--------QEPHL 1854
            :... .||..:.|.:...|||  :.||..                |.|..|:        ...|.
  Rat    80 EGSM-SASAVSGLAALSGPSSGLSSHPCS----------------PVPPGPVTGLRRWLDHSKHC 127

  Fly  1855 IANGPPAFAKDAKESSGNMASPGKMDGNPLSEIEEIVKTTTEQHESNSRVDGGGGV--ENASTNG 1917
            ::....|.:...::....|..|....|..|.|: .::.|..|          |.||  :.|....
  Rat   128 LSVETEADSGQTRQCENWMLEPTLTTGQELPEL-TLLTTLLE----------GPGVKAQPAEEET 181

  Fly  1918 HGRSHDNNDESHTAVSDKAAALKKRQCVFAELMSTEEAYVQDLHEIVNGYMTEINNTNSDIPMPE 1982
            ..::..|.:|      .|..||::...|.:||:.||..||.||.:||.|||.    |.:...:||
  Rat   182 LSQAPKNEEE------QKKTALERSMFVLSELVETERMYVDDLGQIVEGYMA----TMATQGVPE 236

  Fly  1983 DLKGGKMKLVFNNIKDIYEWHRDFFLRALRNCQKSPADLGPLIKRSATKFALYYTYCSNKPLSEY 2047
            .|: |:.::||.||:.|||||||:||:.|:.|.|.|..|..|..:...:..:|..||.|||.||:
  Rat   237 SLR-GRDRIVFGNIQQIYEWHRDYFLQELQQCLKDPDWLAQLFIKHERRLHMYVVYCQNKPKSEH 300

  Fly  2048 IVSAH-YQYFDSIRQKLGHRLDLSNLIIKPVQRITKYELLIKEIIKATEGAGLYKEVPMLQEAYQ 2111
            ::|.. ..||:.:||:|||||.||:|:|||||||.||:||:|:.:|....||:  :...|::|.:
  Rat   301 VLSEFGDSYFEELRQQLGHRLQLSDLLIKPVQRIMKYQLLLKDFLKYYRRAGM--DTEELEQAVE 363

  Fly  2112 QMKVVVKTVNDMMVVLRSLQDFDGEITAQGSLLMQGPMNCVVDAA-----QKHRELQVFLFQQII 2171
            .|..|.|..||||.:.| |:.|:|::||||.||.|.........|     .:.||.:||||:||:
  Rat   364 VMCFVPKRCNDMMSLGR-LRGFEGKLTAQGKLLGQDTFLVTEPEAGGLLSSRGRERRVFLFEQIV 427

  Fly  2172 IFAD-IEKTKNQYASPIFKYRSHIQLNHMQMK---ELGDCRFQIRSTDPKIPEMTIICQAASQEN 2232
            ||:: :.........|.:.|::.|:::.:.::   :...|||.:.|..|:......:.||:....
  Rat   428 IFSEALGGGGRGGTQPGYVYKNSIKVSCLGLEGNLQGNACRFALTSRGPEGGIQRYVLQASDPAV 492

  Fly  2233 YAGWRDMLNKILQQQNDLIFMLSNPLSTKNK 2263
            ...|...:.:||:.|.|.:..|.:|:..:.:
  Rat   493 SQAWIKQVAQILESQRDFLNALQSPIEYQRR 523

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
trio	NP_651960.2	SEC14	13..152	CDD:214706
		SPEC	314..>468	CDD:238103
		SPEC	563..778	CDD:238103
		SPEC	782..997	CDD:238103
		SPEC	897..1119	CDD:238103
		SPEC	1125..1226	CDD:197544
		RhoGEF	1287..1456	CDD:279015
		PH1_Kalirin_Trio_like	1462..1584	CDD:270060
		PH	1495..1580	CDD:278594
		SH3_Kalirin_1	1643..1707	CDD:212786	7/32 (22%)
		RhoGEF	1945..2122	CDD:279015	79/177 (45%)
		PH2_Kalirin_Trio_p63RhoGEF	2130..2260	CDD:270061	38/138 (28%)
Arhgef25	XP_006241516.1	RhoGEF	203..374	CDD:279015	79/177 (45%)
Arhgef25	XP_006241516.1	PH2_Kalirin_Trio_p63RhoGEF	378..520	CDD:270061	39/142 (27%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	148	1.000	Domainoid score	I4334
eggNOG	1	0.900	-	-		E1_KOG0689
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	4	3.770