DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment trio and Trio

DIOPT Version :9

Sequence 1:	NP_651960.2	Gene:	trio / 43974	FlyBaseID:	FBgn0024277	Length:	2263	Species:	Drosophila melanogaster
Sequence 2:	NP_001101128.3	Gene:	Trio / 310192	RGDID:	1308360	Length:	3103	Species:	Rattus norvegicus

Alignment Length:	2339	Identity:	1034/2339 - (44%)
Similarity:	1453/2339 - (62%)	Gaps:	194/2339 - (8%)

- Green bases have known domain annotations that are detailed below.

  Fly     2 DGQRARDLLTLLQERVVFLTGGRDRRGGPLLCFPATPRRDRLKPEDLRRLLSYLISIPSDAAKNL 66
            |..:|.|:|.:|:|:|.:|:||||:||||:|.|||....||::.||||||:|||..|||:.....
  Rat    58 DEMKAMDVLPILKEKVAYLSGGRDKRGGPILTFPARSNHDRMRQEDLRRLISYLACIPSEEVCKR 122

  Fly    67 GFTVIIDMRGNGNCSTNVKTILKVLQEHFSANIHNVVIIKPDNFWQKQRASISSHKYKFETTTVS 131
            |||||:||||:.  ..::|.:||:|||.|...||..:||||||||||||.:..|.|::|||..||
  Rat   123 GFTVIVDMRGSK--WDSIKPLLKILQESFPCCIHIALIIKPDNFWQKQRTNFGSSKFEFETNMVS 185

  Fly   132 IESLNKIVESHQLTGDFEGQQLYDHQQWTDARLAIEDFFWQAGDMADRIDDLQEDLNRNDFAEDV 196
            :|.|.|:|:..|||.:|:|...|:|::|.:.|:|.||:...|..|..|:::||:.|.:.:..:|:
  Rat   186 LEGLTKVVDPSQLTPEFDGCLEYNHEEWIEIRVAFEDYISNAAHMLSRLEELQDVLAKKELPQDL 250

  Fly   197 PLARHAIDHHNEMRKKITKLPIEDLDMQGKKLLAKINAMAPPGGQAPTNSGGPDMDSGPSSAGQQ 261
            ..||:.||.|::::||:.|.||||||::|:|||.:|                           |.
  Rat   251 EGARNMIDEHSQLKKKVIKAPIEDLDLEGQKLLQRI---------------------------QS 288

  Fly   262 SQPSQQSRSVGGNPDMSAAVNKALRQIELIHTGQEKLLMLWQHKKVKLDQCFQWRLFEQDCEKMF 326
            |:...:..|..||.|:...:.|....::.:|:.::.|..:|..:|:|||||||.||||||.||||
  Rat   289 SESFPKKNSGSGNADLQNLLPKVSTMLDRLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMF 353

  Fly   327 DWILHNRDVFQMSYVEIGHNYSVAKSLQDEHQKFAVASMNVSVNIDRILAVAARLIESQHYAAQH 391
            |||.||:.:|..||.|||.::..|..||.:|..||:..|||.|||:||::||.||:||.|||:|.
  Rat   354 DWITHNKGLFLNSYTEIGASHPHAMELQTQHNHFAMNCMNVYVNINRIMSVANRLVESGHYASQQ 418

  Fly   392 IKTLAQRLDRTWKDFAAGLDERTAVLQLSVLFHHKAEQYCNSVASWAAACQASQPLPSDIQSLET 456
            ||.:|.:|::.||.|||.||||:.:|.:|.:||.|||:|.::|.||..|| ....|||::|.||.
  Rat   419 IKQIANQLEQEWKAFAAALDERSTLLDMSSIFHQKAEKYMSNVDSWCKAC-GEVDLPSELQDLED 482

  Fly   457 AIRTHQSLYEAMCQAYTEVHSTSKKLLYQLDHLVQVCNQPPPPGVPDHRKNSNNGSFNKYERQNP 521
            ||..||.:||.:..||:||....|.||.:|       .:|..||..|....|
  Rat   483 AIHHHQGIYEHITLAYSEVSQDGKSLLDKL-------QRPLTPGSSDSLTAS------------- 527

  Fly   522 AADYSEGASHVLAVIHQILSHHRTLEAKWHQERLRLHQTLALRLFQEDVKQVLDWLKNHGEVFLR 586
             |:||:...|||.|||::|.|.|.||..|...::||||.|.|.:||:||:|||||::||||.||.
  Rat   528 -ANYSKAVHHVLDVIHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLS 591

  Fly   587 KNTGIGRNFHKARVYQTSHDNFENVAQNTYSNAQKLLAAADELARSGEADPNEIYSVARELELQV 651
            |:||:|::.|:||..|..|::||.||||||:||.|||.||::||::||.||.|||..|.:||.::
  Rat   592 KHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRI 656

  Fly   652 GSFAERVEQRRRRLDMAVIFYTHEKDVTAWIDKLRTDVSTDETRLSQENLEGIERILQQYQRDQQ 716
            ..|..|||||:..|||:|.|:||.|::..|:::|:.::..|   :..|::|.::.:::::.: ||
  Rat   657 QDFVRRVEQRKILLDMSVSFHTHVKELWTWLEELQKELLDD---VYAESVEAVQDLIKRFGQ-QQ 717

  Fly   717 ESSVNVCLTTISQGEALLQEMRSLEYADN----TGSIAALEATLEKLNKQKVELEELWSARKFRA 777
            ::::.|.:..|.:||.|:|::|....:.|    ..||..:|..|::|::.:.::|||:..||.:.
  Rat   718 QTTLQVTVNVIKEGEDLIQQLRDSAISSNKTPHNSSINHIETVLQQLDEAQSQMEELFQERKIKL 782

  Fly   778 DLILRLRYFERDARLLSVQMEMWSEEL--QHADL-SRDYQKAEQLIRMHNESVTEIQNATYEVVQ 839
            :|.|:||.|||||..:...:|.|::||  |..|. :.|...|||.::.|.:....:.|.|::|:.
  Rat   783 ELFLQLRIFERDAIDIISDLESWNDELSQQMNDFDTEDLTIAEQRLQHHADKALTMNNLTFDVIH 847

  Fly   840 QGQDLLQL---FENAGFISMADATHTAQARIEYLLDFLREREIDLEEMSEAKRAKLEQAVQLCQF 901
            |||||||.   .:.:|...:.|.......|::.||:||.|::.:|:..:|..|..|||.|||...
  Rat   848 QGQDLLQYVNEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDLAAEQHRKHLEQCVQLRHL 912

  Fly   902 QNDANQVISWIRNGEAMLVASFVTPNSLQEAEQLRKGHEQFQIAIEKTHTSAVQVKYRADALINA 966
            |.:..||:.||||||:||.|..:|.:||||||||::.|||||.||||||.||:||:.:|:|::.|
  Rat   913 QAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQREHEQFQHAIEKTHQSALQVQQKAEAMLQA 977

  Fly   967 NHYDPLSIDEISDDVTKKWQQLVTYAEERHKLVTASINFYKTAEQVCSVLDSLEREYKREDDWCD 1031
            ||||...|.:.::.|...||||:...|:|.|||.||:.||||:|||||||:|||:|||||:|||
  Rat   978 NHYDMDMIRDCAEKVASHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWC- 1041

  Fly  1032 GGGGSDK------AQAIVQLISKHQEQKEAFLKACTLARRTAETFLKYANRSQ-----QCYQYKG 1085
              ||:||      ...:..:||||.|||||||||||||||.|:.||||.:|:.     .....|.
  Rat  1042 --GGADKLGPNSETDHVTPMISKHLEQKEAFLKACTLARRNADVFLKYLHRNSVSMPGMVTHIKA 1104

  Fly  1086 NCEGHVKSKLDKLLTQENQVLDYWTLRKKSLDQCQQFVLFERSAKQAIEWIHNTGEAYLSSRSNL 1150
            . |..||:.|::|..:||:||.|||:||:.||||||:|:||||||||:||||:.||.|||:.::
  Rat  1105 P-EQQVKNILNELFQRENRVLHYWTMRKRRLDQCQQYVVFERSAKQALEWIHDNGEFYLSTHTS- 1167

  Fly  1151 VGISKEETEGLLKEHNEFRSTAKETRERVKLLIQLADSLVEKGHAHASDIKQWVASVDQRYKDFS 1215
            .|.|.:.|:.|||||.||:.|||:|:|||||||||||...|||||||::||:.|.:||:||:|||
  Rat  1168 TGSSIQHTQELLKEHEEFQITAKQTKERVKLLIQLADGFCEKGHAHAAEIKKCVTAVDKRYRDFS 1232

  Fly  1216 NRMDSYCEQLEKSLGMSQQQLLLGSDGNSSISSSSGD--RHSDPTLEAKLNSSNKENKEINEEKR 1278
            .||:.|...|||:||:|       ||.|.|..|...|  ..|.|..|.||..:   ..|:|||||
  Rat  1233 LRMEKYRTSLEKALGIS-------SDSNKSSKSLQLDIIPASIPGSEVKLRDA---AHELNEEKR 1287

  Fly  1279 KSARRKEFIMAELMQTERAYVNDLATCIKCFLEEFRAG-KSVPSALIGQEDVIFGNIKEIHHFHQ 1342
            |||||||||||||:|||:|||.||..|:..:|.|..:| :.:|..::.:|.:||||::||:.||.
  Rat  1288 KSARRKEFIMAELIQTEKAYVRDLRECMDTYLWEMTSGVEEIPPGIVNKELIIFGNMQEIYEFHN 1352

  Fly  1343 KIFLRELEKYETMPEDVGHCFVTWASKFDMYVHYCKNKPTSNNLLVQHGGSFFEELQRRLEVDHP 1407
            .|||:||||||.:||||||||||||.||.|||.||||||.|..|:::|.||:|:|:|:|..:.:.
  Rat  1353 NIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSTQLILEHAGSYFDEIQQRHGLANS 1417

  Fly  1408 LPAYLIKPVQRITKYQLLLKDLLSCCEESHGEIKEGLEVMLNVPKKANDAMHLSLLENCDVSVDK 1472
            :.:|||||||||||||||||:||:||||..||||:||||||:|||:||||||||:||..|.:::.
  Rat  1418 ISSYLIKPVQRITKYQLLLKELLTCCEEGKGEIKDGLEVMLSVPKRANDAMHLSMLEGFDENIES 1482

  Fly  1473 LGEVVLQDAFQAWDTKQIIRKGRERRVFLFELYLLFAKEVKESN-VVKYQFKSKLMTTDMGITEH 1536
            .||::||::||.||.|.:|||||||.:||||:.|:|:||||:|: ..||.:||||.|:::|:|||
  Rat  1483 QGELILQESFQVWDPKTLIRKGRERHLFLFEMSLVFSKEVKDSSGRSKYLYKSKLFTSELGVTEH 1547

  Fly  1537 IEGDETKFAVWTGRSPMLSDCRIVLKATSLETKQIWVKKLREVMQETC--FSGT---SLTLPKSP 1596
            :|||..|||:|.||:| .||.:|||||:|:|.||.|:|.:|||:||..  ..|.   .:.:||:.
  Rat  1548 VEGDPCKFALWVGRTP-TSDNKIVLKASSIENKQDWIKHIREVIQERTVHLRGALKEPIHIPKTA 1611

  Fly  1597 AKHSGSSQRSSRDLDEQLT-ENDHDRCSLASFGSGNTTDSDNKLGNQEATWVVADYIATSGSNEL 1660
            .......:|...|||.|.. .:..|..|:||..|.||.|||...|..|.|.|:.|:.|.: ||||
  Rat  1612 PAARQKGRRDGEDLDSQGDGSSQPDTISIASRTSQNTLDSDKLSGGCELTVVIHDFTACN-SNEL 1675

  Fly  1661 SVSKGQQVEIVEPPTAGEPDFCLVRLNPQHDDAAVQEGLVPVSVL--------KPPPGSHKH--- 1714
            ::.:||.||::|.| ..:||:||||..   |.:...|||||...|        ....|...|
  Rat  1676 TIRRGQTVEVLERP-HDKPDWCLVRTT---DRSPAAEGLVPCGSLCIAHSRSSMEMEGIFNHKDS 1736

  Fly  1715 ---GSGTAANAASSAGSQKSDMQDQSNRSKADALSSSTKRRGFSGRNWL--PLMN---------- 1764
               .|..|:..||.|..|...|..||        |...||.|.:.|.||  |:..
  Rat  1737 LSVSSNDASPPASVASLQPHMMGAQS--------SPGPKRPGNTLRKWLTSPVRRLSSGKADGHA 1793

  Fly  1765 RKGTDKPPSSKPLVK-------KPSEKNLRTPQKHAEELAEQQNQQPGASPATVLPSSQFPSSTQ 1822
            :|...|...|:.:.|       |.|:.:..|||   :|..|::.:..|.|..|:..||.
  Rat  1794 KKLAHKHKKSREVRKSADAGSQKDSDDSAATPQ---DETIEERGRNEGLSSGTLSKSSS------ 1849

  Fly  1823 HPGGAGAGDYEPDEEV-GLELPPPMKPIQEPHLIANGPPAFAKDAKESSGNMASPGKMD----GN 1882
             .|....|:.|.:|.. .:.||||| .||:..|:...    ::|.|.||..:..|...:    ..
  Rat  1850 -SGMQSCGEEEGEEGADAVPLPPPM-AIQQHSLLQPD----SQDDKASSRLLVRPTSSETPSAAE 1908

  Fly  1883 PLSEIEEIVKT--TTEQHESNSRVDGGGGVE---NASTNGHGRSHDNNDESHTAVSDKAAALKKR 1942
            .:|.|||:||:  ..|...|:..||.|....   |.|.|....|....||..   ..|.::||:|
  Rat  1909 LVSAIEELVKSKMALEDRPSSLLVDQGDSSSPSFNPSDNSLLSSSSPIDEME---ERKCSSLKRR 1970

  Fly  1943 QCVFAELMSTEEAYVQDLHEIVNGYMTEINNTNSDIPMPEDLKGGKMKLVFNNIKDIYEWHRDFF 2007
            ..|..||:.||..||:||..:|.|||..:....    :|:|:| ||.|:||.||..||:||||||
  Rat  1971 HYVLQELVETERDYVRDLGCVVEGYMALMKEDG----VPDDMK-GKDKIVFGNIHQIYDWHRDFF 2030

  Fly  2008 LRALRNCQKSPADLGPLIKRSATKFALYYTYCSNKPLSEYIVSAHYQ-YFDSIRQKLGHRLDLSN 2071
            |..|..|.:.|..||.|..:...:..:|..||.|||.||:|||.:.. :|:.::|:|||||.|::
  Rat  2031 LGELEKCLEDPEKLGSLFVKHERRLHMYIVYCQNKPKSEHIVSEYIDTFFEDLKQRLGHRLQLTD 2095

  Fly  2072 LIIKPVQRITKYELLIKEIIKATEGAGLYKEVPMLQEAYQQMKVVVKTVNDMMVVLRSLQDFDGE 2136
            |:|||||||.||:||:|:.:|.::.|.|  :...|::|.:.|.:|.|..||||.|.| ||.|||:
  Rat  2096 LLIKPVQRIMKYQLLLKDFLKYSKKASL--DTSELEKAVEVMCIVPKRCNDMMNVGR-LQGFDGK 2157

  Fly  2137 ITAQGSLLMQGPMNCVVD----AAQKHRELQVFLFQQIIIFADIEKTKNQYASPIFKYRSHIQLN 2197
            |.|||.||:|... .|.|    ...:.:|.:||||:||:||::....|..::.|.|.:::.|:::
  Rat  2158 IVAQGKLLLQDTF-LVTDQDAGLLPRCKERRVFLFEQIVIFSEPLDKKKGFSMPGFLFKNSIKVS 2221

  Fly  2198 HMQMK---ELGDCRFQIRSTDPKIPEMTIICQAASQENYAGWRDMLNKILQQQNDLIFMLSNPL 2258
            .:.::   |...|:|.:.|......| |.|..::|......|...:|:||:.|.:.:..|::|:
  Rat  2222 CLCLEENVESDPCKFALTSRTGDAVE-TFILHSSSPSVRQTWIHEINQILENQRNFLNALTSPI 2284

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
trio	NP_651960.2	SEC14	13..152	CDD:214706	76/138 (55%)
		SPEC	314..>468	CDD:238103	84/153 (55%)
		SPEC	563..778	CDD:238103	91/218 (42%)
		SPEC	782..997	CDD:238103	96/220 (44%)
		SPEC	897..1119	CDD:238103	126/232 (54%)
		SPEC	1125..1226	CDD:197544	62/100 (62%)
		RhoGEF	1287..1456	CDD:279015	105/169 (62%)
		PH1_Kalirin_Trio_like	1462..1584	CDD:270060	69/122 (57%)
		PH	1495..1580	CDD:278594	50/85 (59%)
		SH3_Kalirin_1	1643..1707	CDD:212786	28/71 (39%)
		RhoGEF	1945..2122	CDD:279015	78/177 (44%)
		PH2_Kalirin_Trio_p63RhoGEF	2130..2260	CDD:270061	42/136 (31%)
Trio	NP_001101128.3	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..27
		SEC14	69..204	CDD:214706	75/136 (55%)
		SPEC	227..445	CDD:238103	102/244 (42%)
		Spectrin 1	311..418		55/106 (52%)
		SPEC	341..566	CDD:238103	116/246 (47%)
		Spectrin 2	538..644		61/105 (58%)
		SPEC	567..783	CDD:238103	91/219 (42%)
		SPEC	787..1014	CDD:238103	100/226 (44%)
		Spectrin 3	878..984		57/105 (54%)
		SPEC	908..1137	CDD:238103	126/232 (54%)
		Spectrin 4	1109..1216		69/107 (64%)
		SPEC	1143..1243	CDD:197544	62/100 (62%)
		RhoGEF	1295..1465	CDD:238091	105/169 (62%)
		PH1_Kalirin_Trio_like	1472..1594	CDD:270060	69/122 (57%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1601..1651		14/49 (29%)
		SH3_Kalirin_1	1659..1718	CDD:212786	28/63 (44%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1738..1907		50/191 (26%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1927..1956		8/28 (29%)
		RhoGEF	1970..2143	CDD:238091	79/179 (44%)
		PH2_Kalirin_Trio_p63RhoGEF	2148..2286	CDD:270061	44/140 (31%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	2287..2556
		SH3_Kalirin_2	2559..2617	CDD:212787
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	2643..2667
		IG	2696..2780	CDD:214652
		STKc_Trio_C	2792..3054	CDD:271015
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	235	1.000	Domainoid score	I2286
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H20847
Inparanoid	1	1.050	1737	1.000	Inparanoid score	I62
OMA	1	1.010	-	-		QHG46230
OrthoDB	1	1.010	-	-		D4513at33208
OrthoFinder	1	1.000	-	-		FOG0002562
OrthoInspector	1	1.000	-	-		otm46332
orthoMCL	1	0.900	-	-		OOG6_103664
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X1686
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	12	11.880