Protein Alignment trio and Pura

DIOPT Version :10

• Sequence 1: NP_651960.2
• Gene: trio / 43974
• FlyBaseID: FBgn0024277
• Length: 2263
• Species: Drosophila melanogaster
• Sequence 2: NP_996016.2
• Gene: Pura / 2768948
• FlyBaseID: FBgn0035802
• Length: 1904
• Species: Drosophila melanogaster
• Alignment Length: 1974
• Identity: 402/1974 - (20%)
• Similarity: 684/1974 - (34%)
• Gaps: 650/1974 - (32%)

  Fly    86 TILKVLQEHFSANIHNVVIIKPDNFWQKQRASISSHKYK-----FETTTVSIES--LNKIVE--- 140
            |:.:::..|.| :|:.|       :|.:.||.:.....:     |:.:.|..|.  |.|..:
  Fly   322 TLARLIDSHLS-HIYPV-------YWARTRAILIQQARERLVCGFDGSLVRFEQRFLCKFAQIAA 378

  Fly   141 ----SHQLTGDFEGQQLYDHQQW-------------TDARLA----IEDFFWQAGDMADRIDDLQ 184
                :||:     |:....|..|             :||.:|    ..:.:     :..:::..:
  Fly   379 TLRTAHQI-----GESWLWHPGWPLATSNGALVLQLSDAEIAHALRPSELY-----LCIKLESTE 433

  Fly   185 EDLN---------RNDFAEDVPLARHAIDHHNEMRKKITKLPIEDLDMQGKKLLAKINAMAPPGG 240
            .|.:         |:...|: .:..|.|||.|.     .:||:..|.::.....|.:.|
  Fly   434 SDADCKPRLYAVWRSSRKEE-SVQSHCIDHENP-----GELPLSVLQLEMSTAAAALMA------ 486

  Fly   241 QAPTNSGGPDMDSGPSSAGQQSQPSQQSRSVGGNPDMSAAVNKALRQIELIHTGQEKLLMLWQHK 305
                           |..|:..|..|         ::..:|.:::.::.|     .:|.|...|.
  Fly   487 ---------------SDCGELPQLLQ---------NLLVSVERSIERVSL-----NELQMPMHHD 522

  Fly   306 KVK--LDQ-----CFQWRLFEQDCE-KMFDWILHNRDVFQMSYVEIGHNYSVAKSLQDEHQKFAV 362
            :.:  ||:     |....|....|. :..|.|..|: :|...|:     .....:.||.....:.
  Fly   523 RGEDSLDEANNNGCNNNLLGSPKCALRRSDLITKNK-LFNSRYM-----LRRLTNRQDSMNSTSS 581

  Fly   363 ASMNVSVNIDRILAVAARLIESQHYAAQHIKTLAQRLDRTWKDFAAGLDERTAVLQLSVLFHHKA 427
            .:.|||   ||..:.::   .|...|.:.:.|                            .|.:|
  Fly   582 GNSNVS---DRSSSSSS---SSSSSAGEELLT----------------------------SHSEA 612

  Fly   428 EQYCNSVASWAAACQASQPLP--------SDIQSLETAIRT-----HQSLYEAMCQAYTEVHSTS 479
            :...|||.:.|:....|..||        ..|.|.|.|..:     |.||  ..|:  ||:..:|
  Fly   613 KMRSNSVGNEASGASTSPALPLYCLGNSFPHIDSDEEASDSIGPGKHHSL--ETCE--TELLPSS 673

  Fly   480 ------KKLL-----------YQLDHLVQV---CNQPPPPGVPDHRKNSNNGSFNKYERQNPAAD 524
                  :|||           .|.|.||.|   |              ..:...|.||.......
  Fly   674 SIAELPEKLLTSGVYLPGTRTLQGDPLVTVDAAC--------------VASAGINCYEMATLLLY 724

  Fly   525 YS---EGASHVLAV-----IHQILSHHRTLEAKWHQERLRLHQ---------TLALRLFQEDVKQ 572
            ||   |.:|.....     ....::.|::.|.:..|::.:..|         |:.:.:.:.....
  Fly   725 YSTIPERSSCPFTTPATPGTTATINGHKSTEQQQQQQQQQQQQKQQPAQQTFTILIAIEKSQHLC 789

  Fly   573 VLDWLKNHGEVFLRKNTG-----------------IGRNFHKARVYQTSHDN------------- 607
            |:|.:.....: |.|..|                 .|:...|.:..|..::|
  Fly   790 VIDLICQSLRL-LAKQIGNCEILAVCPDSSLLQLCNGQQQQKLQQQQQQNNNSDHPEPSSPSPVD 853

  Fly   608 ---FENVAQNTYSNAQK-----LLAAADELAR-SGEADPNEIYSVARELELQVGSFAERVEQRRR 663
               |.:|.|.|.|.|..     ||.:...|:. .|.:..:...:..||...|:.:|..:......
  Fly   854 LVKFISVDQVTTSVAPSQLPAGLLPSVPALSTLPGISQRHHDATKWREFFAQLEAFQRQCSTAGG 918

  Fly   664 RLDMAVIFYTHEKDVTAWIDKLRTDVSTDETRLSQENLEGIERILQQYQRDQQESSVNVCLTTIS 728
            ||            |:|..:....|:....||   ..|.|..|.|.:...|.|..|    |..:.
  Fly   919 RL------------VSALSEIRAADLQGLPTR---RQLYGQHRALSRALMDSQLHS----LRKMG 964

  Fly   729 QGE-ALLQEM-RSLEYA---------------DNTGSIAALEAT---------LEKLNKQKVELE 767
            .|: |.||:: |::..|               .::|:...::|.         ..::::....||
  Fly   965 AGQLARLQDLARAITSAPGSPRMTSQPQSSSSSSSGAFLNVDAACKLRKVTLLFNEVDRAAQRLE 1029

  Fly   768 ELWSARKFRADLILRLRYFERDARLLSVQMEMWSEELQHADLSRDYQKAEQLIRMHNESVTEIQN 832
            :|...|:.|...:.|.|..|.:..    ::..|    ..:|.:.:.||..|| ...|||....|.
  Fly  1030 QLTEQRRERLRQLTRQRALEDEIS----EVTSW----MGSDGAENLQKFSQL-HWDNESQLRTQE 1085

  Fly   833 ATYEVVQQGQDLLQLFENAGFISMADATHTAQARIEYLLDFLREREIDLEEMSEAK---RAKLEQ 894
                         |.||...||:|   .|.|:.|      .|....:.:..:||:.   ::.|:|
  Fly  1086 -------------QEFEKYYFIAM---KHLAKGR------DLHAAALKVSVLSESANNLKSALDQ 1128

  Fly   895 AVQLCQFQNDANQVISWIRNGEAMLVASFVTPNSLQEA--EQLRKGHEQFQI---------AIEK 948
            ..|..:..::        |...|..:...:|.:..:.|  |:|::..||...         .:.|
  Fly  1129 FAQKLELAHE--------RYDAAGRLLHLLTQHQRENAAREELQRLAEQLGATSLLEKHWPVMRK 1185

  Fly   949 THTSAV-----------QVKYRADALINANHYDPLSIDEISDDVTKKWQQLVTYAEERHKLVTAS 1002
            |||..|           :|.||..:...::.....|....|           .:.|.|:
  Fly  1186 THTLPVASSTPAAAAGKRVSYRCSSASRSSFEGGPSSSNCS-----------CWRESRN------ 1233

  Fly  1003 INFYKTAEQVCSVLDSLEREYKREDDWCDGGGGSDKAQAIVQLISKHQEQKEAFLKACTLARRTA 1067
                      ...:|.:|.|.:.:|..||||.|.::...|.          ::.|..|
  Fly  1234 ----------LEDMDQMEEEEEEQDHDCDGGDGEEQQSKIA----------DSGLGVC------- 1271

  Fly  1068 ETFLKYANRSQQCYQYKGNCEGHVKSKLDKLLTQENQVLDYWTLRKKSLDQCQQFVLFERSAKQA 1132
                             .|||.:  .||.::.:.:       :|.:||.|:..:...|:|.:|:.
  Fly  1272 -----------------DNCERN--PKLARICSCQ-------SLNEKSHDELLEDECFDRPSKRY 1310

  Fly  1133 IEWIHNTGE--AYLSSRSNLVGISKEETEGLLKEHNEFRSTAKETRERVKLLIQLADSLVEKGHA 1195
            ::.:|:..|  |:|....:.:.:.|.|.
  Fly  1311 MDIMHSPMEANAHLQCHGSSLELPKLEE------------------------------------- 1338

  Fly  1196 HASDIKQWVASVDQRYKDFSNRMDSYCEQLEKSLGMSQQQLLLGSDGNSSISSSSGDRHSDPTLE 1260
                    ::.:|.:.                     |:.|||
  Fly  1339 --------LSCLDPKI---------------------QKTLLL---------------------- 1352

  Fly  1261 AKLNSSNKENKEINEEKRKSARRKEFIMAELMQTERAYVNDLATCIKCFLEEFRAGKSVPSALIG 1325
                                      ||.|::.|||.||..|...|:.:::|. ..:.:|..|.|
  Fly  1353 --------------------------IMREMIGTERDYVRSLYYVIENYIDEL-LREDIPQPLRG 1390

  Fly  1326 QEDVIFGNIKEIHHFHQKIFLRELEKYETMPEDVGHCFVTWASKFDMYVHYCKNKPTSNNLLVQH 1390
            |.:||||||::|..||...||.|||:||..|..||..|:...|||.:|..|.||||.|:.||.::
  Fly  1391 QRNVIFGNIEKIFEFHNSHFLGELERYERNPLKVGAAFLEMESKFYLYALYNKNKPKSDTLLSEY 1455

  Fly  1391 GGSFFEELQRRLEVDHPLPAYLIKPVQRITKYQLLLKDLLSCCEESHG-----------EIKEGL 1444
            |.|||:..|.:|:....|.:||:|||||:.||.|||:.|:..|:...|           |::...
  Fly  1456 GSSFFKPKQMQLQDKLDLASYLLKPVQRMGKYALLLQQLVKACKGVEGAALQEIAADVEELQRAE 1520

  Fly  1445 EVMLNVPKKANDAMHLSLLENCDVSVDKLGEVVLQDAFQAWDTKQIIRKGRE--RRVFLFELYLL 1507
            |::....:..||.:.:..|.:|||:|.:.|.::.|:.|..|..    |.|::  |:|||||..:|
  Fly  1521 EMVKFQLRHGNDLLAMDSLRDCDVNVKEQGRLLRQNEFLVWQG----RGGKKTLRQVFLFEELVL 1581

  Fly  1508 FAKEVK---ESNVVKYQFKSKLMTTDMGITEHIEGDETKFAVW-TGRSPMLSDCRIVLKATSLET 1568
            |:|..:   ..|:..|.:|:.:.|:|:|:|.|.....|||.:| ..|.|   |...:|:..|.:.
  Fly  1582 FSKARRFPDHKNLDIYIYKNSIKTSDIGLTAHTGDSATKFEIWFRKRKP---DDTWMLQCMSEDI 1643

  Fly  1569 KQIWVKKLREVMQETCFSGTSLTLPKSPAKHSGS----------SQRSSRDLD-EQLTEND---H 1619
            |..|.:::.:::.:.......:.|.:..:...||          :|.|.|.:. .||.:..   |
  Fly  1644 KNAWTEEISKLLWKQAKRNREVRLAEMSSMGIGSKPCLDIRPSNNQISDRSIPLAQLNKTPKLRH 1708

  Fly  1620 DRCSLASFGSGNTTDSDNKLGNQEATWVVADYIATSGSNELSVSKGQQVEIVEPPTAGEPDFCLV 1684
            ......|....|:..|::.|.:..:|        ||||   |:|.|.        ::|
  Fly  1709 SEPGKGSMRRPNSLISESSLSSGTST--------TSGS---SISGGS--------SSG------- 1747

  Fly  1685 RLNPQHDDAAVQEGLVPVSVLKPPPGSHKHGSGTAANAASSAGSQKSDMQDQSNRSKADALSSST 1749
                 |:|..               |.|....|....|.|::.:..|...:..|.::|..|:.|.
  Fly  1748 -----HNDGG---------------GRHSLHFGKLPGAHSASSTNCSATLELINETQALKLAKSP 1792

  Fly  1750 KRRGFSGRNWLPLMNRKGTDKPPSSKPLVKKPSEKNLRTPQKHAEELAEQQNQQPG-ASPATVLP 1813
            ...|..|.:  ...:...|....:......:.|::|    .|.:..:..|.:.:.| .|...|.|
  Fly  1793 SGGGIKGAD--TAADGGNTSGNGNGNGNAFRSSKRN----HKRSTTIVSQLSMESGIVSDMCVTP 1851

  Fly  1814 --------SSQFPSSTQHPGGAGA 1829
                    ||:...||...|.:||
  Fly  1852 DQEQSAAESSRSSWSTSTTGASGA 1875

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
trio	NP_651960.2	SEC14	13..152	CDD:214706	16/79 (20%)
		SPEC	314..>468	CDD:238103	33/167 (20%)
		SPEC	563..778	CDD:238103	51/279 (18%)
		SPEC	782..997	CDD:238103	48/239 (20%)
		SPEC	897..1119	CDD:238103	42/243 (17%)
		SPEC	1125..1226	CDD:197544	10/102 (10%)
		RhoGEF	1287..1455	CDD:459876	71/178 (40%)
		PH1_Kalirin_Trio_like	1462..1584	CDD:270060	39/127 (31%)
		SH3_Kalirin_1	1643..1707	CDD:212786	11/63 (17%)
		RhoGEF	1945..2121	CDD:459876
		PH2_Kalirin_Trio_p63RhoGEF	2130..2260	CDD:270061
Pura	NP_996016.2	COG4913	<1020..>1167	CDD:443941	39/185 (21%)
		RhoGEF	1353..1524	CDD:459876	71/171 (42%)
		PH_puratrophin-1	1524..1663	CDD:270062	41/145 (28%)