Protein Alignment trio and Tiam2

DIOPT Version :10

• Sequence 1: NP_651960.2
• Gene: trio / 43974
• FlyBaseID: FBgn0024277
• Length: 2263
• Species: Drosophila melanogaster
• Sequence 2: NP_036008.2
• Gene: Tiam2 / 24001
• MGIID: 1344338
• Length: 1715
• Species: Mus musculus
• Alignment Length: 1813
• Identity: 335/1813 - (18%)
• Similarity: 588/1813 - (32%)
• Gaps: 669/1813 - (36%)

  Fly   188 NRNDFAEDVPLARHAIDHHNEMRKKITKLP-------IEDLDMQGKK--LLAKINAMAPPGG--- 240
            ||..||.|:.:... :||.:.:......||       .||   ..||  |.|::...:...|
Mouse   329 NRVSFASDMDVPSR-VDHRDPLHYSSFTLPCRKSKALTED---AAKKDTLKARMRRFSDWTGSLS 389

  Fly   241 ------QAPTNSGGPD-MDSGPSSAGQQSQPSQQSRSVGGNPDMSAAVNKALRQIELIHTGQEKL 298
                  |.|.:..|.: .||.......:.|...|:.|
Mouse   390 RKKRKLQEPRSMEGSEYFDSHSDGLNAEGQVPAQTSS---------------------------- 426

  Fly   299 LMLWQHKKVKLDQCFQWRLFEQDCEKMFDWILHNRDVFQMSYVEIGHNYSVAKSLQDEHQKFAVA 363
             :||.....:                    .|.:|.       |..|..||....|:.::.|
Mouse   427 -LLWSGGSAQ--------------------TLPHRS-------ESTHAISVDPLRQNIYENF--- 460

  Fly   364 SMNVSVNIDRILAVAARLIESQHYAAQHIKTLAQRLDRTWKDFAAGLDERTAVLQLSVLFHHKAE 428
                           .|.:|......:|::|..:.::       :..:..:::.||.:||  :.|
Mouse   461 ---------------MRELEMSRSNTEHVETSTETME-------SSSESVSSLEQLDLLF--EKE 501

  Fly   429 QYCNSVASWAAACQASQPLPS--DIQSLETAIRTHQS------------LYEAMCQAYTEVHSTS 479
            |.....|.|.    ..:||.:  ..:.||...|....            .||...:..||.:|..
Mouse   502 QGVVRKAGWL----FFKPLVTLQKERKLELVARRKWKQYWVTLKGCTLLFYETYGKNSTEQNSAP 562

  Fly   480 KKLLYQLDHLVQVCNQPPPPGVPDHRKNSNNGSFNKYERQNPAADYSEGASHVLAVIHQILSHHR 544
            :..|:..|.:||        .||:|.|.                      .||..:.:..
Mouse   563 RCALFAEDSIVQ--------SVPEHPKK----------------------EHVFCLSNSC----- 592

  Fly   545 TLEAKWHQERLRLHQTLALRLFQEDVKQVLDWLKNHGEVFLRKNTGIGRNFHKARVYQ-TSHDNF 608
                                                |:|:|               :| ||..:.
Mouse   593 ------------------------------------GDVYL---------------FQATSQTDL 606

  Fly   609 ENVAQNTYSNAQKLLAAADELARSGEADPNEIYSVARELELQVGSFAERVEQRRRRLDMAVIFYT 673
            ||.....:|....|.|     .:.|:.|      ..|.|:.|..|..::::...:...||
Mouse   607 ENWVTAIHSACASLFA-----KKHGKED------TVRLLKSQTRSLLQKIDMDSKMKKMA----- 655

  Fly   674 HEKDVTAWIDKLRTDVSTDETRLSQENLEGIERILQQYQRDQQESSVNV----C-LTTISQGEAL 733
                      :|:..|.:|     .:|.:.||..::|::::.::..:::    | |.::..||
Mouse   656 ----------ELQLSVVSD-----PKNRKAIENQIRQWEQNLEKFHMDLFRMRCYLASLQGGE-- 703

  Fly   734 LQEMRSLEYADNTGSIAAL-------------------EATLEK----LNKQKVELEELWSARKF 775
            |...:||..|.:..|..||                   ::||.|    |.::....:.::|:.|.
Mouse   704 LPNPKSLLAATSRPSKLALGRLGVLSVSSFHALVCSRDDSTLRKRTLSLTQRGKSKKGIFSSLKG 768

  Fly   776 RADLILRLRYFERDARLLSVQMEMWSEELQHADL-------SRDYQKAEQLIRMHNESV-TEIQN 832
            ...|..:.|  |:.|.:    .:|:.....|..|       |.:..||..| .::..:| :.:::
Mouse   769 LDTLARKGR--EKRASI----TQMFDSSHSHGFLGTQLPQKSTNSNKAHDL-HLYGSAVDSALRD 826

  Fly   833 ATYEVVQQGQDLLQLFENAGFISMADATHTAQARIEYLLDFL-REREIDLEEMSEAKRAKLEQAV 896
            :.:||    |..:...:|.|    ...|...:.|:|.:|..: :.|:::........|..::::|
Mouse   827 SMWEV----QTYVHFQDNEG----VTVTIKPEHRVEDVLALVCKMRQLEPTHYGLQLRKVVDKSV 883

  Fly   897 QLC---QFQNDANQVISWIRNGEAMLVASFVTPNSLQEAEQLRKGH-EQFQIAIEKTHTSAVQVK 957
            :.|   .::....||...|.          |.|.|:.:.:..:.|. ..|..|:      .|||.
Mouse   884 EWCVPALYEYMQEQVYDEIE----------VFPLSVYDVQLTKTGDMTDFGFAV------TVQVD 932

  Fly   958 YRADALINANHYDPLSIDEISDDVTKKWQQLVTYAEERHKLVTASINFYKTAEQVCSVLDSLERE 1022
                   ...|.:.:.|.::..|                     |:.:
Mouse   933 -------EHQHLNRIFISDVLPD---------------------SLAY----------------- 952

  Fly  1023 YKREDDWCDGGGGSDKAQAIVQLISK-----HQEQKEAFLK----ACTLARRTAETFLKYANRSQ 1078
                      |||..|...|..|..:     ..:|.||...    ..||..|...|      |..
Mouse   953 ----------GGGLRKGNEITSLNGEPVSDLDIQQMEALFSEKSVGLTLVARPVTT------RRT 1001

  Fly  1079 QCYQYKGNCEGHVKSKLDKLLTQENQVLDYWTLRKKSLDQC-QQFVLFERSAKQAIEWIHNTGEA 1142
            .|..:..:                    |.::..:|||... .|..|.|       |::.|..:.
Mouse  1002 LCASWSDS--------------------DLFSRDQKSLPPSPNQSQLLE-------EFLDNFRKT 1039

  Fly  1143 YLSSRSNL----VGISKEETEGLLKE--HNEFRSTAKETRERVKLLIQLADSLVEKGHAHASDIK 1201
            ..|..||:    .|:.:.:|||.|.:  |          ||:::.....||.:.|
Mouse  1040 ATSDFSNVPEITTGLKRSQTEGTLDQVPH----------REKMEQTFLSADQIAE---------- 1084

  Fly  1202 QWVASVDQRYKDFSNRMDSYCEQLEKSLGMSQQQLLLGSDGNSSISSSSGDRHSDPTLEAKLNSS 1266
                               .|..|..:                         |:: ::||...|.
Mouse  1085 -------------------LCRDLNNT-------------------------HTN-SMEAPTESH 1104

  Fly  1267 NKENKEINEEKRKSARRKEFIMAELMQTERAYVNDLATCIKCFLEEFRAGKSVPSALIGQEDV-- 1329
            :...:.:......:.|.:: ::.||:.||::||.||:...:.:||..:     ....:.|:::
Mouse  1105 DPPPRPLARHLSDADRLRK-VIQELVDTEKSYVKDLSCLFELYLEPLQ-----NETFLTQDEMES 1163

  Fly  1330 IFGNIKEIHHFHQKIFLRELE----------------KYETMPEDVGHCFVTWASKFDMYVHYCK 1378
            :||::.|:..| ||:||..||                ::..:...:|..|:.:|..|.:|..:|
Mouse  1164 LFGSLPEMLEF-QKVFLETLEDAISASSDFSVLETPSQFRKLLFSLGGSFLYYADHFKLYSGFC- 1226

  Fly  1379 NKPTSNNLLVQHGGSFFEELQRR---------LEVDHP-------LPAYLIKPVQRITKYQLLLK 1427
                :|::.||      ..|:|.         |:..:|       |.:||||||||:.||.||||
Mouse  1227 ----ANHIKVQ------RVLERAKTDKAFKAFLDARNPTKQHSSTLESYLIKPVQRVLKYPLLLK 1281

  Fly  1428 DLLSCCE---ESHGEIKEGLEVMLNVPKKANDAMHLSLLENCDVSVDKL---------------- 1473
            :|:|..:   |.|..:.|.|:.|..|....|:..  .:.|:..:..|:|
Mouse  1282 ELVSLTDHESEEHYHLTEALKAMEKVASHINEMQ--KIYEDYGMVFDQLVAEQSGTEKEVTELSM 1344

  Fly  1474 GEVVLQDAFQAWDTKQI----IRKGRERRVFLFE--LYLLFAKEVK--------------ESNVV 1518
            ||:::.... :|....:    .||..|..||:|:  :.|::.:..|              .:::.
Mouse  1345 GELLMHSTV-SWLNPFLSLGKARKDIELTVFVFKRAVILVYKENCKLKKKLPSNSRPAHNSADLD 1408

  Fly  1519 KYQFK----SKLMTTDMGITEHIEGDETKFAVWT-----GRSPMLSDCRIVLKATSLETKQIWVK 1574
            .::|:    ...:...:|.|...|.:.|...:.|     ||...:    ..|..:..|.|...||
Mouse  1409 PFKFRWLIPISALQVRLGNTAGTENNSTWELIHTKSEIEGRPETI----FQLCCSDSENKTSIVK 1469

  Fly  1575 KLREVMQET------CFSGTSLTLPKS------PAKHS---GSSQRSSRDLDEQLTENDHDRCSL 1624
            .:|.:::|.      |    .|.|.|:      |.|:.   .:...|||.|....|.:..:..|.
Mouse  1470 VIRSILRENFRRHIKC----ELPLEKTCKDRLVPLKNRVPVSAKLASSRSLKGLRTSSSSEWPSE 1530

  Fly  1625 ASFGSGNTTDSDN---KLGNQEATWVVADYIATSGSNELSVSKGQQVEIVE-------------- 1672
            .|  .||:.|||.   ..|.|.:...||:....|.|.||  .|..|..:.|
Mouse  1531 PS--KGNSLDSDECSLSSGTQSSGCPVAESRRDSKSTEL--EKDAQEGLAEFPDGLIKESDILSD 1591

  Fly  1673 -------PPTAGEP------DFCLVRLNPQHD------DAAVQEGLVPVSV------LKPPPGSH 1712
                   |...|.|      .|..::::.:.|      ....:.|..|..|      :|....|.
Mouse  1592 EDEDFHHPLKQGSPTKDIEIQFQRLKISEESDVHPVGQQPLTESGEQPKLVRGHFCPIKRKANST 1656

  Fly  1713 KHGSGTAANAASSAGSQKSDMQDQSNRSKADALS---SSTKRRGFSGRNWLPLMNRKG 1767
            |.|.||..         |:..:.||..|..:..|   :....|.||.::...::|.:|
Mouse  1657 KRGRGTLL---------KAQTRHQSLDSHPETASIDLNLVLEREFSVQSLTSVVNEEG 1705

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
trio	NP_651960.2	SEC14	13..152	CDD:214706
		SPEC	314..>468	CDD:238103	27/167 (16%)
		SPEC	563..778	CDD:238103	43/243 (18%)
		SPEC	782..997	CDD:238103	39/227 (17%)
		SPEC	897..1119	CDD:238103	36/234 (15%)
		SPEC	1125..1226	CDD:197544	18/106 (17%)
		RhoGEF	1287..1455	CDD:459876	57/204 (28%)
		PH1_Kalirin_Trio_like	1462..1584	CDD:270060	29/172 (17%)
		SH3_Kalirin_1	1643..1707	CDD:212786	17/102 (17%)
		RhoGEF	1945..2121	CDD:459876
		PH2_Kalirin_Trio_p63RhoGEF	2130..2260	CDD:270061
Tiam2	NP_036008.2	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..27
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	174..249
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	263..294
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	385..418		6/32 (19%)
		PH1_Tiam1_2	503..629	CDD:269937	34/220 (15%)
		Tiam_CC_Ex	643..740	CDD:408184	20/118 (17%)
		RBD	832..902	CDD:128731	13/73 (18%)
		PDZ_canonical	911..994	CDD:467153	21/143 (15%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1092..1113		4/46 (9%)
		RhoGEF	1124..1313	CDD:214619	57/205 (28%)
		PH2_Tiam1_2	1315..1487	CDD:269957	30/182 (16%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1515..1582		20/70 (29%)