DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment trio and Trio

DIOPT Version :9

Sequence 1:	NP_651960.2	Gene:	trio / 43974	FlyBaseID:	FBgn0024277	Length:	2263	Species:	Drosophila melanogaster
Sequence 2:	NP_001074771.1	Gene:	Trio / 223435	MGIID:	1927230	Length:	3103	Species:	Mus musculus

Alignment Length:	2336	Identity:	1030/2336 - (44%)
Similarity:	1456/2336 - (62%)	Gaps:	188/2336 - (8%)

- Green bases have known domain annotations that are detailed below.

  Fly     2 DGQRARDLLTLLQERVVFLTGGRDRRGGPLLCFPATPRRDRLKPEDLRRLLSYLISIPSDAAKNL 66
            |..:|.|:|.:|:|:|.:|:||||:||||:|.|||....||::.||||||:|||..|||:.....
Mouse    58 DEMKAMDVLPILKEKVAYLSGGRDKRGGPILTFPARSNHDRIRQEDLRRLISYLACIPSEEVCKR 122

  Fly    67 GFTVIIDMRGNGNCSTNVKTILKVLQEHFSANIHNVVIIKPDNFWQKQRASISSHKYKFETTTVS 131
            |||||:||||:.  ..::|.:||:|||.|...||..:||||||||||||.:..|.|::|||..||
Mouse   123 GFTVIVDMRGSK--WDSIKPLLKILQESFPCCIHIALIIKPDNFWQKQRTNFGSSKFEFETNMVS 185

  Fly   132 IESLNKIVESHQLTGDFEGQQLYDHQQWTDARLAIEDFFWQAGDMADRIDDLQEDLNRNDFAEDV 196
            :|.|.|:|:..|||.:|:|...|:|::|.:.|:|.|::...|..|..|:::||:.|.:.:..:|:
Mouse   186 LEGLTKVVDPSQLTPEFDGCLEYNHEEWIEIRVAFEEYISNAAHMLSRLEELQDVLAKKELPQDL 250

  Fly   197 PLARHAIDHHNEMRKKITKLPIEDLDMQGKKLLAKINAMAPPGGQAPTNSGGPDMDSGPSSAGQQ 261
            ..||:.||.|::::||:.|.||||||::|:|||.:|                           |.
Mouse   251 EGARNMIDEHSQLKKKVIKAPIEDLDLEGQKLLQRI---------------------------QS 288

  Fly   262 SQPSQQSRSVGGNPDMSAAVNKALRQIELIHTGQEKLLMLWQHKKVKLDQCFQWRLFEQDCEKMF 326
            |....:..|..||.|:...:.|....::.:|:.::.|..:|..:|:|||||||.||||||.||||
Mouse   289 SDSFPKKNSGSGNADLQNLLPKVSTMLDRLHSTRQHLHQMWHVRKLKLDQCFQLRLFEQDAEKMF 353

  Fly   327 DWILHNRDVFQMSYVEIGHNYSVAKSLQDEHQKFAVASMNVSVNIDRILAVAARLIESQHYAAQH 391
            |||.||:.:|..||.|||.::..|..||.:|..||:..|||.|||:||::||.||:||.|||:|.
Mouse   354 DWITHNKGLFLNSYTEIGTSHPHAMELQTQHNHFAMNCMNVYVNINRIMSVANRLVESGHYASQQ 418

  Fly   392 IKTLAQRLDRTWKDFAAGLDERTAVLQLSVLFHHKAEQYCNSVASWAAACQASQPLPSDIQSLET 456
            ||.:|.:|::.||.|||.||||:.:|.:|.:||.|||:|.::|.||..|| ....|||::|.||.
Mouse   419 IKQIANQLEQEWKAFAAALDERSTLLDMSSIFHQKAEKYMSNVDSWCKAC-GEVDLPSELQDLED 482

  Fly   457 AIRTHQSLYEAMCQAYTEVHSTSKKLLYQLDHLVQVCNQPPPPGVPDHRKNSNNGSFNKYERQNP 521
            ||..||.:||.:..||:||....|.||.:|       .:|..||..|....|
Mouse   483 AIHHHQGIYEHITLAYSEVSQDGKSLLDKL-------QRPLTPGSSDSLTAS------------- 527

  Fly   522 AADYSEGASHVLAVIHQILSHHRTLEAKWHQERLRLHQTLALRLFQEDVKQVLDWLKNHGEVFLR 586
             |:||:...|||.|||::|.|.|.||..|...::||||.|.|.:||:||:|||||::||||.||.
Mouse   528 -ANYSKAVHHVLDVIHEVLHHQRQLENIWQHRKVRLHQRLQLCVFQQDVQQVLDWIENHGEAFLS 591

  Fly   587 KNTGIGRNFHKARVYQTSHDNFENVAQNTYSNAQKLLAAADELARSGEADPNEIYSVARELELQV 651
            |:||:|::.|:||..|..|::||.||||||:||.|||.||::||::||.||.|||..|.:||.::
Mouse   592 KHTGVGKSLHRARALQKRHEDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYQAAHQLEDRI 656

  Fly   652 GSFAERVEQRRRRLDMAVIFYTHEKDVTAWIDKLRTDVSTDETRLSQENLEGIERILQQYQRDQQ 716
            ..|..|||||:..|||:|.|:||.|::..|:::|:.::..|   :..|::|.::.:::::.: ||
Mouse   657 QDFVRRVEQRKILLDMSVSFHTHVKELWTWLEELQKELLDD---VYAESVEAVQDLIKRFGQ-QQ 717

  Fly   717 ESSVNVCLTTISQGEALLQEMRSLEYADN----TGSIAALEATLEKLNKQKVELEELWSARKFRA 777
            ::::.|.:..|.:||.|:|::|....:.|    ..||..:|..|::|::.:.::|||:..||.:.
Mouse   718 QTTLQVTVNVIKEGEDLIQQLRDSAISSNKTPHNSSINHIETVLQQLDEAQSQMEELFQERKIKL 782

  Fly   778 DLILRLRYFERDARLLSVQMEMWSEEL--QHADL-SRDYQKAEQLIRMHNESVTEIQNATYEVVQ 839
            :|.|:||.|||||..:...:|.|::||  |..|. :.|...|||.::.|.:....:.|.|::|:.
Mouse   783 ELFLQLRIFERDAIDIISDLESWNDELSQQMNDFDTEDLTIAEQRLQHHADKALTMNNLTFDVIH 847

  Fly   840 QGQDLLQL---FENAGFISMADATHTAQARIEYLLDFLREREIDLEEMSEAKRAKLEQAVQLCQF 901
            |||||||.   .:.:|...:.|.......|::.||:||.|::.:|:..:|..|..|||.|||...
Mouse   848 QGQDLLQYVNEVQASGVELLCDRDVDMATRVQDLLEFLHEKQQELDLAAEQHRKHLEQCVQLRHL 912

  Fly   902 QNDANQVISWIRNGEAMLVASFVTPNSLQEAEQLRKGHEQFQIAIEKTHTSAVQVKYRADALINA 966
            |.:..||:.||||||:||.|..:|.:||||||||::.|||||.||||||.||:||:.:|:|::.|
Mouse   913 QAEVKQVLGWIRNGESMLNAGLITASSLQEAEQLQREHEQFQHAIEKTHQSALQVQQKAEAMLQA 977

  Fly   967 NHYDPLSIDEISDDVTKKWQQLVTYAEERHKLVTASINFYKTAEQVCSVLDSLEREYKREDDWCD 1031
            ||||...|.:.::.|...||||:...|:|.|||.||:.||||:|||||||:|||:|||||:|||
Mouse   978 NHYDMDMIRDCAEKVASHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYKREEDWC- 1041

  Fly  1032 GGGGSDK------AQAIVQLISKHQEQKEAFLKACTLARRTAETFLKYANRSQQCYQYKGNC--- 1087
              ||:||      ...:..:||||.|||||||||||||||.|:.||||.:|:.  ....|..
Mouse  1042 --GGADKLGPNSETDHVTPMISKHLEQKEAFLKACTLARRNADVFLKYLHRNS--VSMPGMVTHI 1102

  Fly  1088 ---EGHVKSKLDKLLTQENQVLDYWTLRKKSLDQCQQFVLFERSAKQAIEWIHNTGEAYLSSRSN 1149
               |..||:.|::|..:||:||.|||:||:.||||||:|:||||||||:||||:.||.|||:.::
Mouse  1103 KAPEQQVKNILNELFQRENRVLHYWTMRKRRLDQCQQYVVFERSAKQALEWIHDNGEFYLSTHTS 1167

  Fly  1150 LVGISKEETEGLLKEHNEFRSTAKETRERVKLLIQLADSLVEKGHAHASDIKQWVASVDQRYKDF 1214
             .|.|.:.|:.|||||.||:.|||:|:|||||||||||...|||||||::||:.|.:||:||:||
Mouse  1168 -TGSSIQHTQELLKEHEEFQITAKQTKERVKLLIQLADGFCEKGHAHAAEIKKCVTAVDKRYRDF 1231

  Fly  1215 SNRMDSYCEQLEKSLGMSQQQLLLGSDGNSSISSSSGD--RHSDPTLEAKLNSSNKENKEINEEK 1277
            |.||:.|...|||:||:|       ||.|.|..|...|  ..|.|..|.||..:   ..|:||||
Mouse  1232 SLRMEKYRTSLEKALGIS-------SDSNKSSKSLQLDIIPASIPGSEVKLRDA---AHELNEEK 1286

  Fly  1278 RKSARRKEFIMAELMQTERAYVNDLATCIKCFLEEFRAG-KSVPSALIGQEDVIFGNIKEIHHFH 1341
            ||||||||||||||:|||:|||.||..|:..:|.|..:| :.:|..::.:|.:||||::||:.||
Mouse  1287 RKSARRKEFIMAELIQTEKAYVRDLRECMDTYLWEMTSGVEEIPPGIVNKELIIFGNMQEIYEFH 1351

  Fly  1342 QKIFLRELEKYETMPEDVGHCFVTWASKFDMYVHYCKNKPTSNNLLVQHGGSFFEELQRRLEVDH 1406
            ..|||:||||||.:||||||||||||.||.|||.||||||.|..|:::|.||:|:|:|:|..:.:
Mouse  1352 NNIFLKELEKYEQLPEDVGHCFVTWADKFQMYVTYCKNKPDSTQLILEHAGSYFDEIQQRHGLAN 1416

  Fly  1407 PLPAYLIKPVQRITKYQLLLKDLLSCCEESHGEIKEGLEVMLNVPKKANDAMHLSLLENCDVSVD 1471
            .:.:|||||||||||||||||:||:||||..||||:||||||:|||:||||||||:||..|.:::
Mouse  1417 SISSYLIKPVQRITKYQLLLKELLTCCEEGKGEIKDGLEVMLSVPKRANDAMHLSMLEGFDENIE 1481

  Fly  1472 KLGEVVLQDAFQAWDTKQIIRKGRERRVFLFELYLLFAKEVKESN-VVKYQFKSKLMTTDMGITE 1535
            ..||::||::||.||.|.:|||||||.:||||:.|:|:||||:|: ..||.:||||.|:::|:||
Mouse  1482 SQGELILQESFQVWDPKTLIRKGRERHLFLFEMSLVFSKEVKDSSGRSKYLYKSKLFTSELGVTE 1546

  Fly  1536 HIEGDETKFAVWTGRSPMLSDCRIVLKATSLETKQIWVKKLREVMQETC--FSGT---SLTLPKS 1595
            |:|||..|||:|.||:| .||.:|||||:|:|.||.|:|.:|||:||..  ..|.   .:.:||:
Mouse  1547 HVEGDPCKFALWVGRTP-TSDNKIVLKASSIENKQDWIKHIREVIQERTVHLRGALKEPIHIPKT 1610

  Fly  1596 PAKHSGSSQRSSRDLDEQLT-ENDHDRCSLASFGSGNTTDSDNKLGNQEATWVVADYIATSGSNE 1659
            ........:|...|||.|.. .:..|..|:||..|.||.|||...|..|.|.|:.|:.|.: |||
Mouse  1611 APAARQKGRRDGEDLDSQGDGSSQPDTISIASRTSQNTLDSDKLSGGCELTVVIHDFTACN-SNE 1674

  Fly  1660 LSVSKGQQVEIVEPPTAGEPDFCLVRLNPQHDDAAVQEGLVPVSVL--------KPPPG--SHKH 1714
            |::.:||.||::|.| ..:||:||||..   |.:...|||||...|        ....|  :||.
Mouse  1675 LTIRRGQTVEVLERP-HDKPDWCLVRTT---DRSPAAEGLVPCGSLCIAHSRSSMEMEGIFNHKD 1735

  Fly  1715 GSGTAANAASSAGSQKSDMQDQSNRSKADALSSSTKRRGFSGRNWL--PLMN----------RKG 1767
            ....::|.||...|..| :|.....:::   |...||.|.:.|.||  |:..          :|.
Mouse  1736 SLSVSSNDASPPASVAS-LQPHMIGAQS---SPGPKRPGNTLRKWLTSPVRRLSSGKADGHAKKL 1796

  Fly  1768 TDKPPSSKPLVK-------KPSEKNLRTPQKHAEELAEQQNQQPGASPATVLPSSQFPSSTQHPG 1825
            ..|...|:.:.|       |.|:.:..|||   :|..|::.:..|.|..|:..||.       .|
Mouse  1797 AHKHKKSREVRKSADAGSQKDSDDSAATPQ---DETIEERGRNEGLSSGTLSKSSS-------SG 1851

  Fly  1826 GAGAGDYEPDEEV-GLELPPPMKPIQEPHLIANGPPAFAKDAKESSGNMASPGKMD----GNPLS 1885
            ....|:.|.:|.. .:.||||| .||:..|:...    ::|.|.||..:..|...:    ...:|
Mouse  1852 MQSCGEEEGEEGADAVPLPPPM-AIQQHSLLQPD----SQDDKASSRLLVRPTSSETPSAAELVS 1911

  Fly  1886 EIEEIVKT--TTEQHESNSRVDGGGGVE---NASTNGHGRSHDNNDESHTAVSDKAAALKKRQCV 1945
            .|||:||:  ..|...|:..||.|....   |.|.|....|....||..   ..|.::||:|..|
Mouse  1912 AIEELVKSKMALEDRPSSLLVDQGDSSSPSFNPSDNSLLSSSSPIDEME---ERKCSSLKRRHYV 1973

  Fly  1946 FAELMSTEEAYVQDLHEIVNGYMTEINNTNSDIPMPEDLKGGKMKLVFNNIKDIYEWHRDFFLRA 2010
            ..||:.||..||:||..:|.|||..:....    :|:|:| ||.|:||.||..||:|||||||..
Mouse  1974 LQELVETERDYVRDLGCVVEGYMALMKEDG----VPDDMK-GKDKIVFGNIHQIYDWHRDFFLGE 2033

  Fly  2011 LRNCQKSPADLGPLIKRSATKFALYYTYCSNKPLSEYIVSAHYQ-YFDSIRQKLGHRLDLSNLII 2074
            |..|.:.|..||.|..:...:..:|..||.|||.||:|||.:.. :|:.::|:|||||.|::|:|
Mouse  2034 LEKCLEDPEKLGSLFVKHERRLHMYIVYCQNKPKSEHIVSEYIDTFFEDLKQRLGHRLQLTDLLI 2098

  Fly  2075 KPVQRITKYELLIKEIIKATEGAGLYKEVPMLQEAYQQMKVVVKTVNDMMVVLRSLQDFDGEITA 2139
            ||||||.||:||:|:.:|.::.|.|  :...|::|.:.|.:|.|..||||.|.| ||.|||:|.|
Mouse  2099 KPVQRIMKYQLLLKDFLKYSKKASL--DTSELEKAVEVMCIVPKRCNDMMNVGR-LQGFDGKIVA 2160

  Fly  2140 QGSLLMQGPMNCVVD----AAQKHRELQVFLFQQIIIFADIEKTKNQYASPIFKYRSHIQLNHMQ 2200
            ||.||:|... .|.|    ...:.:|.:||||:||:||::....|..::.|.|.:::.|:::.:.
Mouse  2161 QGKLLLQDTF-LVTDQDAGLLPRCKERRVFLFEQIVIFSEPLDKKKGFSMPGFLFKNSIKVSCLC 2224

  Fly  2201 MK---ELGDCRFQIRSTDPKIPEMTIICQAASQENYAGWRDMLNKILQQQNDLIFMLSNPL 2258
            ::   |...|:|.:.|......| |.:..::|......|...:|:||:.|.:.:..|::|:
Mouse  2225 LEENVESDPCKFALTSRTGDAVE-TFVLHSSSPSVRQTWIHEINQILENQRNFLNALTSPI 2284

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
trio	NP_651960.2	SEC14	13..152	CDD:214706	76/138 (55%)
		SPEC	314..>468	CDD:238103	84/153 (55%)
		SPEC	563..778	CDD:238103	91/218 (42%)
		SPEC	782..997	CDD:238103	96/220 (44%)
		SPEC	897..1119	CDD:238103	126/233 (54%)
		SPEC	1125..1226	CDD:197544	62/100 (62%)
		RhoGEF	1287..1456	CDD:279015	105/169 (62%)
		PH1_Kalirin_Trio_like	1462..1584	CDD:270060	69/122 (57%)
		PH	1495..1580	CDD:278594	50/85 (59%)
		SH3_Kalirin_1	1643..1707	CDD:212786	28/71 (39%)
		RhoGEF	1945..2122	CDD:279015	78/177 (44%)
		PH2_Kalirin_Trio_p63RhoGEF	2130..2260	CDD:270061	41/136 (30%)
Trio	NP_001074771.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..27
		SEC14	69..204	CDD:214706	75/136 (55%)
		Spectrin 1	218..338		41/146 (28%)
		SPEC	220..445	CDD:238103	103/251 (41%)
		Spectrin 2	340..446		61/105 (58%)
		SPEC	341..566	CDD:238103	116/246 (47%)
		Spectrin 3	566..672		60/105 (57%)
		SPEC	567..783	CDD:238103	91/219 (42%)
		Spectrin 4	673..784		30/114 (26%)
		SPEC	787..1014	CDD:238103	100/226 (44%)
		Spectrin 5	907..1012		57/104 (55%)
		SPEC	908..1137	CDD:238103	126/233 (54%)
		Spectrin 6	1138..1244		66/106 (62%)
		SPEC	1143..1243	CDD:197544	62/100 (62%)
		RhoGEF	1295..1465	CDD:238091	105/169 (62%)
		PH1_Kalirin_Trio_like	1472..1594	CDD:270060	69/122 (57%)
		PH	1505..1590	CDD:278594	50/85 (59%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1601..1651		14/49 (29%)
		SH3_Kalirin_1	1659..1718	CDD:212786	28/63 (44%)
		SH3-RhoG_link	1715..1972	CDD:293215	72/278 (26%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1781..1906		33/139 (24%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1927..1957		8/29 (28%)
		RhoGEF	1970..2143	CDD:238091	79/179 (44%)
		PH2_Kalirin_Trio_p63RhoGEF	2148..2286	CDD:270061	43/140 (31%)
		PH	2175..2271	CDD:278594	23/96 (24%)
		SH3_Kalirin_2	2559..2617	CDD:212787
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	2643..2666
		I-set	2689..2780	CDD:254352
		Ig	2703..2780	CDD:299845
		STKc_Trio_C	2792..3054	CDD:271015
		S_TKc	2800..3054	CDD:214567
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	235	1.000	Domainoid score	I2354
eggNOG	1	0.900	-	-		E1_KOG4240
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H20847
Inparanoid	1	1.050	1771	1.000	Inparanoid score	I68
Isobase	1	0.950	-	0	Normalized mean entropy	S5434
OMA	1	1.010	-	-		QHG46230
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0002562
OrthoInspector	1	1.000	-	-		otm44233
orthoMCL	1	0.900	-	-		OOG6_103664
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3053
SonicParanoid	1	1.000	-	-		X1686
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	14	13.710