Protein Alignment trio and cgef-1

DIOPT Version :10

• Sequence 1: NP_651960.2
• Gene: trio / 43974
• FlyBaseID: FBgn0024277
• Length: 2263
• Species: Drosophila melanogaster
• Sequence 2: NP_741736.2
• Gene: cgef-1 / 180571
• WormBaseID: WBGene00006496
• Length: 1055
• Species: Caenorhabditis elegans
• Alignment Length: 1166
• Identity: 233/1166 - (19%)
• Similarity: 402/1166 - (34%)
• Gaps: 363/1166 - (31%)

  Fly   739 SLEYADNTGSIAALEATLEKLNKQKVELEELWSARKFRADLILRLRYFERDARLLSVQMEMWSEE 803
            |.|...:|.|...:..::.|.|.|.:|      |..|         :::.|..|..::     ..
 Worm    16 SEEVTTSTLSRGPMTMSVSKSNSQFIE------APAF---------FYDMDTDLKDLK-----TA 60

  Fly   804 LQHADLSRD--YQKAEQLIRMHNESVTEIQNATYEVVQQGQDLLQLFENAGFISMADATHTAQAR 866
            |:..||..|  |:..             ::.:|:.....    |...|:.||.|..|||.
 Worm    61 LKDVDLLNDSGYRSV-------------MERSTHPFATS----LVFHEDNGFDSEDDATD----- 103

  Fly   867 IEYLLDFLR---EREIDLEEMSEAKRAKLEQAVQLCQFQNDANQVISWIRNGEAMLVASFVTPNS 928
             |:...:|.   :| |.:.:||:....:.              ..||..|..|.:.:.:|  |:|
 Worm   104 -EHWDGYLTVGGDR-IKIRDMSDILATRY--------------AFISGARTTEGLTIVTF--PDS 150

  Fly   929 ---------------LQEAEQLRKGHEQFQIAIEKTHTS-------------------------- 952
                           |.:...|...|:.|.|.|::....
 Worm   151 RSTLPFEDYSLLVKYLLQVPPLEDSHKGFVIIIDRRSDKWSSVRTLLLQISSFFPGKICVTFVIK 215

  Fly   953 -------AVQVKYRADALINANHYDPLSIDEISDDVTKKWQQLVTYAEER----HKLVTASIN-- 1004
                   |::|.||..|       |..|...|         ||.:.||.|    |:.:|..:.
 Worm   216 PEGVLQRALEVGYRGAA-------DTCSFQVI---------QLESSAELRKYIHHEFLTMDVGGL 264

  Fly  1005 -FYKTAEQVCSVLDSLER----------------EYKREDDWCDGGGGSDKAQAIVQLISKHQEQ 1052
             .|...|.|...:| :||                :..||.:.      .:.|.:..:::.....:
 Worm   265 IKYNHLEWVQHRMD-IERMKASATAIAASLTEFGKTLRETEL------PNDADSTARILELQTAE 322

  Fly  1053 KEAFLKACTLARRTAETFLKYANRSQQCYQYKGNCEG------HVKSKLDKLLTQ----ENQVLD 1107
            |.|..:...:|.|...|.||...:..:    |...|.      |..:.::::|.|    |.....
 Worm   323 KMAIKEEFKIAVRKGFTLLKSVRQLDK----KPTPEQLSPTRLHNVTAIERMLVQLEETERSFDS 383

  Fly  1108 YWTLRKKSLDQCQQFVLFERSAK--QAIEWIHNTGEAYLSSRSNLVGISKEETEGLLKEHNEFRS 1170
            :||..::.|..|.:...||.|.:  ||....|   ..||..... ||...|:...|.::|.::..
 Worm   384 FWTKHERRLRYCLELRQFEDSFRKLQAAFARH---MLYLEEHRE-VGNCTEKALHLAEQHRQYAE 444

  Fly  1171 TAKETRERVKLLIQLADSLVEKGHAHAS--------DIKQWVASVDQRYK--------------- 1212
            .|.|..|..:.|.:..:.|:....|..|        ::::..|::....:
 Worm   445 GAMEEVEASRNLKKAGEDLINSNEAELSGSLEPKCEELERMAAALTSALERRGDCLKRSATMHEQ 509

  Fly  1213 --------------------DFSN--------RMDSYCEQ--------LEKSLGMSQQQLLLGSD 1241
                                ||:|        .:|::.|:        |:.:.....|.|||.:.
 Worm   510 IAKANSWCTRGADLLTNGMTDFTNPNPSSSLTTLDAFIEEGTNLNIEFLKDTTSPMNQLLLLTTI 574

  Fly  1242 GNSSISSSSGDRHSD---------------------------PTLEAKLNSSNKE--NKEINE-- 1275
            ..|::.:...:|..|                           |..::|.|||..|  :|::..
 Worm   575 ETSTLLNLIEERIGDIRRMSLAKRDQLTKLHLQKPPPVQVVTPEKKSKKNSSKNELDSKDVPSCS 639

  Fly  1276 ---------EKRKSARRKEFIMAELMQTERAYVNDLATCIKCFLEEFRAGK---SVPSALIGQED 1328
                     ..|:..|.......|::.||.:||.||...|..:||.|.|.:   |:|..|.|:.|
 Worm   640 GESTPAGTLSPRRETREVTNAFDEMIATEISYVADLKDIIIHYLEPFEATENQNSLPDTLRGKPD 704

  Fly  1329 VIFGNIKEIHHFHQKIFLRELEKYETMPEDVGHCFVTWASKFDMYV---HYC------------- 1377
            .:|||::|::.||.:..|.:|....:..|   .|.|....:..:||   .||
 Worm   705 CLFGNVRELYKFHHRTVLEDLVAARSTAE---MCRVLMQHRNQIYVTYRTYCQIHGSNQKVRDSV 766

  Fly  1378 KNKPTSNNLLVQHGGSFFEELQRRLEVDHPLPAYLIKPVQRITKYQLLLKDLL-SCCEESHGEI- 1440
            ||.|            ||:|.||....:..:.:||:||:|||.||||||.::: .|..:...|:
 Worm   767 KNHP------------FFKECQRNANHNMDMSSYLLKPIQRIMKYQLLLGNIMDDCPADVRDEVA 819

  Fly  1441 --KEGLEVMLNVPKKANDAMHLSLLENCDVSVDKLGEVVLQ---DAFQAWDTKQI-IRKGRERRV 1499
              ::.:..:||....:...:|:|   ..:..:..||.:.||   |.|.....|:. :.:.::|.:
 Worm   820 MTRDSMVELLNQIDASMQQLHIS---GYNGDLKSLGLLRLQTECDVFTYNRKKKAKLSRAQKRFI 881

  Fly  1500 FLFELYLLFAKE-------VKESNVVKYQFKSKLMTTDMGITEHIEGDETKFAVWTGRSPMLSDC 1557
            |.|:..::|.|:       ...|....::.|..:....:|.........::|.||       .|.
 Worm   882 FFFDGAVMFCKKRVSNPGTTLNSEPEYFEHKFCIPIISLGYDTSSRTGASRFEVW-------DDA 939

  Fly  1558 R---IVLKATSLETKQIWVKKL--REVMQETCFSGTSLTLPKSPAKHSGSSQRSSRDLDEQLTEN 1617
            :   .|::......:..|:::|  .|..|:.|.....  .|||.|.                |.:
 Worm   940 KTDAYVIETIDQTARTKWIQRLGKSETAQDACLENRQ--RPKSWAS----------------TVS 986

  Fly  1618 DHDRCSLASFGSGNTTDSDNKLGNQEATWVVADYIATSGSNELSVSKGQQVEIVEPPTAGE 1678
            :...||.::..|.:|..:.:..||.:.|.|...|..|..|   |:....|::...|....|
 Worm   987 NESSCSSSTRESDSTDSTMDTNGNTQTTQVDVPYSPTLDS---SIDLSFQMDTTTPLRTSE 1044

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
trio	NP_651960.2	SEC14	13..152	CDD:214706
		SPEC	314..>468	CDD:238103
		SPEC	563..778	CDD:238103	10/38 (26%)
		SPEC	782..997	CDD:238103	48/271 (18%)
		SPEC	897..1119	CDD:238103	53/302 (18%)
		SPEC	1125..1226	CDD:197544	26/161 (16%)
		RhoGEF	1287..1455	CDD:459876	57/190 (30%)
		PH1_Kalirin_Trio_like	1462..1584	CDD:270060	24/137 (18%)
		SH3_Kalirin_1	1643..1707	CDD:212786	9/36 (25%)
		RhoGEF	1945..2121	CDD:459876
		PH2_Kalirin_Trio_p63RhoGEF	2130..2260	CDD:270061
cgef-1	NP_741736.2	CRAL_TRIO_2	142..269	CDD:463965	25/144 (17%)
		RhoGEF	658..833	CDD:238091	57/189 (30%)
		PH-like	841..968	CDD:473070	24/136 (18%)