DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment trio and Mcf2l

DIOPT Version :9

Sequence 1:	NP_651960.2	Gene:	trio / 43974	FlyBaseID:	FBgn0024277	Length:	2263	Species:	Drosophila melanogaster
Sequence 2:	NP_001357978.1	Gene:	Mcf2l / 17207	MGIID:	103263	Length:	1178	Species:	Mus musculus

Alignment Length:	1292	Identity:	297/1292 - (22%)
Similarity:	482/1292 - (37%)	Gaps:	364/1292 - (28%)

- Green bases have known domain annotations that are detailed below.

  Fly   710 QYQRDQQESSVNVCLTTISQGEALLQEMRSLE-YADNTGSIAALEATLEKLNKQKVELEELWSAR 773
            ::.||:.:..|.|         .:|..:..|. |.|.:.....|..||:..:.:       |...
Mouse   152 KFNRDEFKMKVPV---------MMLSSVPELHGYIDKSQLTEDLGGTLDYCHSR-------WLCH 200

  Fly   774 KFRADLILRLRYFERDARLLSVQMEMWSEELQHADLSRDYQKAEQLIRMHNESVTEIQNATYEVV 838
            :      ..:..|....:..:..::.:..||...:|..|.|....::..|.|...:::......:
Mouse   201 R------TAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEKKAKVKEDLQLAL 259

  Fly   839 QQGQDLLQ-----LFENAGFISMADATHTAQARIEYLLDFLREREIDLEEMSEAKRAKLEQAVQL 898
            ::|..:|:     |.|:|......|.... ||.::.||..|.|.|...:|.....:.||||.:||
Mouse   260 KEGNSILESLREPLAESAAHSVNQDQLDN-QATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQL 323

  Fly   899 CQFQNDANQVISWIRNGEAMLVASFVTPNSLQEAEQLRKG----HEQFQIAIEKTHTSAVQVKYR 959
            ..|:....:|.:.:.:....:.|.....|||...:.|.|.    .|:..:|::|....:::
Mouse   324 RHFEQGFREVKTTLDSMSQKIAAFTDVGNSLAHVQHLLKDLTAFEEKSSVAVDKARALSLE---- 384

  Fly   960 ADALINANHYDPLSI-----------DEISDDVTKKWQQLVTYAEERHKLVTASINFYKTAEQVC 1013
            ...||...||...||           |..:.:||:: :.|::.:.|.|.|:..|:.
Mouse   385 GQQLIENRHYAVDSIHPKCEELQHLCDHFASEVTRR-RGLLSKSLELHSLLETSMK--------- 439

  Fly  1014 SVLDSLEREYKREDDWCDGGGGSDKAQAIVQLISKHQEQKEAFLKACTLARRTAETFLKYANRSQ 1078
                           |.|.|                     .||.|
Mouse   440 ---------------WSDEG---------------------IFLLA------------------- 449

  Fly  1079 QCYQYKGNCEGHVKSKLDKLLTQENQVLDYWTLRKKSLDQCQQFVLFERSAKQAIEWIHNTGEAY 1143
                         ...:||..:|:.        .:.:|.:.::|:  |..|:..|:.::   |.|
Mouse   450 -------------SQPVDKCQSQDG--------AEAALQEIEKFL--ETGAENKIQELN---EIY 488

  Fly  1144 LSSRSNLVGISKEETEGLLKEHNEFRSTAKETRERVKLLIQLADSLVEKGHAHASDIKQWVASVD 1208
                        :|.|.:|.:         :..|.|:.:.|..:|..|..|...:.:|:..|...
Mouse   489 ------------KEYECILNQ---------DLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQT 532

  Fly  1209 QRYKDFSNRMDSYCEQLEKSLGMSQQQLLLGSDGNSSISSSSGDR-HSDPTLEAKLNSSNK---E 1269
            :..:..:.|.    |.|.||...|.     ||..:|..|||.|:. ...|...||...|..   .
Mouse   533 RPVQPVAPRP----EALTKSPSPSP-----GSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQGR 588

  Fly  1270 NKEINEEKRKSARRKEFIMAELMQTERAYVNDLATCIKCFLEEFRAGKSVP-------SALIGQE 1327
            .....||:...|..:..:|.||:.||||||.:|.    |.||.:.|....|       :.|..::
Mouse   589 TSSTGEEEESLAILRRHVMNELLDTERAYVEELL----CVLEGYAAEMDNPLMAHLISTGLQNKK 649

  Fly  1328 DVIFGNIKEIHHFHQKIFLRELEKYETMPEDVGHCFVTWASKFDMYVHYCKNKPTSNNLLVQHGG 1392
            :::|||::||:|||.:|||||||.....||.||.||:....:|.:|..||:|||.|.:|..|...
Mouse   650 NILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSD 714

  Fly  1393 -SFFEELQRRLEVDHPLPAYLIKPVQRITKYQLLLKDLLSCCEESHG--EIKEGLEVMLNVPKKA 1454
             .||:|.|::|:....|.:||:||||||||||||||::|...:...|  :::|.|..:|.:.|..
Mouse   715 CPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILKAV 779

  Fly  1455 NDAMHLSLLENCDVSVDKLGEVVLQDAFQAW--------DTKQIIR-KGRERRVFLFELYLLFAK 1510
            ||:|||..:...|.::..||::::|.:|..|        ..|::.| |..:|.:||.|..:||.|
Mouse   780 NDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLFCK 844

  Fly  1511 EVKES-----NVVKYQFKSKLMTTDMGITEHIEGDETKFAVW-TGRSPMLSDCRIVLKATSLETK 1569
            :.:|:     ....|.:|..|..|.:||||:::||..||.:| ..|..:     .:::|.:.|.|
Mouse   845 KREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEV-----YIIQAPTPEIK 904

  Fly  1570 QIWVKKLREVMQETCFSGTSLTLPKSPAKHSGSSQRSSRDLDEQLTENDHDRCSLASFGSGNTTD 1634
            ..||.::|:|:.      :.|...:..::|....|..|..|...         |..|...|||.:
Mouse   905 AAWVNEIRKVLT------SQLQACREASQHRALEQSHSLPLPTP---------SSTSPTKGNTRN 954

  Fly  1635 SDNKLGNQEATWVVADYIATSGSNELSVSKGQQVEIVEPPTAGEPDFCLVRLNPQHDDAAVQEGL 1699
                                                            :.:|..:..|....||.
Mouse   955 ------------------------------------------------VKKLEDRKTDPLSLEGY 971

  Fly  1700 VPVSVLKPPPGSHKHGSGTAANAASSAGSQKSDMQDQSNRSKADALSSSTKRRGFSGRNWLPLM- 1763
            |..|:.|||                             .:.|.||:.|||.......|....|.
Mouse   972 VSSSLPKPP-----------------------------EKGKDDAVPSSTSESSALSRKRFTLQG 1007

  Fly  1764 --NRKGTDKPPSSKPLVKKPSEKNLRTP--------QKHAEELAEQQNQQPGASPATVLPSSQFP 1818
              |.|.:...|..|  .|:...|:..||        ..|:.|..|:..   |.|.|..|.:|
Mouse  1008 LANLKASPTSPDKK--AKRHEVKSDPTPFGLRGWSKTSHSLEAPEEDG---GWSSAEELINS--- 1064

  Fly  1819 SSTQHPGGAGAGDYEP-------DEEVGLELPPPMKPIQEPHLIANGPPAFAKDAKESSGNMA-- 1874
            |..:..||.|.....|       |:|.|                  ||...|.    .||:|.
Mouse  1065 SDAEEDGGVGPKKLVPGKYTVVMDDEKG------------------GPDTLAM----RSGDMVEV 1107

  Fly  1875 --------------SPGKMDGNPLSEIEEIV-KTTTEQHESNSRVDGGGGVENASTN 1916
                          :..|....|.|.:..:: |:::.|..|:|....|..|.::|::
Mouse  1108 VEEGAEGLWYVRDLTSSKEGWVPASSLSTLLGKSSSAQCLSSSDSSPGSAVLSSSSS 1164

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
trio	NP_651960.2	SEC14	13..152	CDD:214706
		SPEC	314..>468	CDD:238103
		SPEC	563..778	CDD:238103	12/68 (18%)
		SPEC	782..997	CDD:238103	50/234 (21%)
		SPEC	897..1119	CDD:238103	37/236 (16%)
		SPEC	1125..1226	CDD:197544	18/100 (18%)
		RhoGEF	1287..1456	CDD:279015	75/178 (42%)
		PH1_Kalirin_Trio_like	1462..1584	CDD:270060	38/136 (28%)
		PH	1495..1580	CDD:278594	28/90 (31%)
		SH3_Kalirin_1	1643..1707	CDD:212786	6/63 (10%)
		RhoGEF	1945..2122	CDD:279015
		PH2_Kalirin_Trio_p63RhoGEF	2130..2260	CDD:270061
Mcf2l	NP_001357978.1	SEC14	46..191	CDD:214706	10/47 (21%)
		SPEC	322..524	CDD:238103	52/317 (16%)
		RhoGEF	603..780	CDD:238091	75/180 (42%)
		PH_Dbs	787..918	CDD:269934	38/141 (27%)
		SH3_DBS	1082..1136	CDD:212791	12/75 (16%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG4240
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.810