DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment trio and Mcf2l

DIOPT Version :9

Sequence 1:	NP_651960.2	Gene:	trio / 43974	FlyBaseID:	FBgn0024277	Length:	2263	Species:	Drosophila melanogaster
Sequence 2:	XP_006253478.1	Gene:	Mcf2l / 117020	RGDID:	619782	Length:	1280	Species:	Rattus norvegicus

Alignment Length:	1563	Identity:	355/1563 - (22%)
Similarity:	573/1563 - (36%)	Gaps:	461/1563 - (29%)

- Green bases have known domain annotations that are detailed below.

  Fly   496 PPPPGVPDHRKNSNN-----------GSFNKYERQN----PAADYSEGASHVLAVIHQILSHHRT 545
            |.||..|....|:..           ||..|  |.|    |||.......|..|.:.|    .||
  Rat    30 PLPPSAPRSPINAKRAPLPVARRPILGSQGK--RHNPLPAPAAPPPHAQPHPTAPLVQ----RRT 88

  Fly   546 LEAKWH----QERLRL---HQTLALRLFQEDVKQVLDWLKNHGEVF---------------LRK- 587
            :  .|.    ..|:|.   ::.:||   :|.|:::....||..|:.               |:|
  Rat    89 I--FWGSVLLSRRMRFWLRNEEMAL---EEMVQRLNAVSKNTDEIMHQDIVPLCAADIQEQLKKR 148

  Fly   588 ----NTGIGRNFHKARVYQTSHDNFENVAQNTYSNAQKLLAAADELARSGEADPNEIYSVARELE 648
                :.|.|::......: ..:..|..:....:.|....|.:...|..:|
  Rat   149 FAYLSGGRGQDGSPVITF-PDYPAFSEIPDKEFQNVMTYLTSIPSLQDAG--------------- 197

  Fly   649 LQVGSFAERVEQRRRRLDMAVIFYTHEKDVTAWIDKLRTDVSTDETRLSQE---NLEGI------ 704
              :| |...:::|:.:                |     |.|.....|::..   ||:.:
  Rat   198 --IG-FILVIDRRQDK----------------W-----TSVKASVLRIAASFPANLQLVLVLRPT 238

  Fly   705 ---ERILQ----QYQRDQQESSVNVCLTTISQGEALLQEMRSLE-YADNTGSIAALEATLEKLNK 761
               :|.|.    ::.||:.:..|.|         .:|..:..|. |.|.:.....|..||:..:.
  Rat   239 GFFQRTLSDIAFKFNRDEFKMKVPV---------MMLSSVPELHGYIDKSQLTEDLGGTLDYCHS 294

  Fly   762 QKVELEELWSARKFRADLILRLRYFERDARLLSVQMEMWSEELQHADLSRDYQKAEQLIRMHNES 826
            :       |...:      ..:..|....:..:..::.:..||...:|..|.|....::..|.|.
  Rat   295 R-------WLCHR------TAIESFALMVKQTAQMLQAFGTELAETELPNDVQSTSLVLSAHTEK 346

  Fly   827 VTEIQNATYEVVQQGQDLLQLFENAGFISMADA-THTA-------QARIEYLLDFLREREIDLEE 883
            ..:::......:.:|..:|:....    .:|:: .|:.       ||.::.||..|.|.|...:|
  Rat   347 KAKVKEDLQLALTEGNSILESLRE----PLAESIVHSVNQDQLDNQATVKRLLTQLNETEAAFDE 407

  Fly   884 MSEAKRAKLEQAVQLCQFQNDANQVISWIRNGEAMLVASFVTPNSLQEAEQLRKGHEQFQIAIEK 948
            .....:.||||.:||..|:....:|.:.:.:....:.|.....|||...:.|.|....|:   ||
  Rat   408 FWAKHQQKLEQCLQLRHFEQGFREVKTALDSMSQKIAAFTDVGNSLAHVQHLLKDLTTFE---EK 469

  Fly   949 THTSAVQV-KYRADALINANHYDPLSIDEISDDVTKKWQQLVTYAEERHKLVTASINFYKTAEQV 1012
               |:|.| |.||           ||::         .|||:   |.||..|.   :.:...|::
  Rat   470 ---SSVAVDKARA-----------LSLE---------GQQLI---ENRHYAVD---SIHPKCEEL 505

  Fly  1013 CSVLDSLEREYKREDDWCDGGGGSDKAQAIVQLISKHQEQKEAFLKACTLARRTAETFLKYANRS 1077
            ..:.|....|..|..|                |:||..|           .....||.:|:::
  Rat   506 QHLCDHFASEVTRRRD----------------LLSKSLE-----------LHSLLETSMKWSD-- 541

  Fly  1078 QQCYQYKGNCEGHVKSKLDKLLTQENQVLDYWTLRKKSLDQCQQFVLFERSAKQAIEWIHNTGEA 1142
                      ||                  .:.|..:.:|:||.....| :|.|.||....||
  Rat   542 ----------EG------------------IFLLASQPVDKCQSQDGAE-AALQEIEKFLETG-- 575

  Fly  1143 YLSSRSNLVGISKEETEGLLKEHNEFRSTA-KETRERVKLLIQLADSLVEKGHAHASDIKQWVAS 1206
                       ::.:.:.|.|.:.|:.... ::..|.|:.:.|..:|..|..|...:.:|:..|.
  Rat   576 -----------AENKIQELNKIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAK 629

  Fly  1207 VDQRYKDFSNRMDSYCEQLEKSLGMSQQQLLLGSDGNSSISSSSGDR-HSDPTLEAKLNSSNK-- 1268
            ..:..:..:.|.    |.|.||...|.     ||..:|..|||.|:. ...|...||...|..
  Rat   630 QTRPVQPVAPRP----EALTKSPSPSP-----GSWRSSENSSSEGNALRRGPYRRAKSEMSEPRQ 685

  Fly  1269 -ENKEINEEKRKSARRKEFIMAELMQTERAYVNDLATCIKCFLEEFRAGKSVP-------SALIG 1325
             ......||:...|..:..:|.||:.||||||.:|.    |.||.:.|....|       :.|..
  Rat   686 GRTSSTGEEEESLAILRRHVMNELLDTERAYVEELL----CVLEGYAAEMDNPLMAHLISTGLQN 746

  Fly  1326 QEDVIFGNIKEIHHFHQKIFLRELEKYETMPEDVGHCFVTWASKFDMYVHYCKNKPTSNNLLVQH 1390
            :::::|||::||:|||.:|||||||.....||.||.||:....:|.:|..||:|||.|.:|..|.
  Rat   747 KKNILFGNMEEIYHFHNRIFLRELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQC 811

  Fly  1391 GG-SFFEELQRRLEVDHPLPAYLIKPVQRITKYQLLLKDLLSCCEESHG--EIKEGLEVMLNVPK 1452
            .. .||:|.|::|:....|.:||:||||||||||||||::|...:...|  :::|.|..:|.:.|
  Rat   812 SDCPFFQECQKKLDHKLSLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAEDLQEALSSILGILK 876

  Fly  1453 KANDAMHLSLLENCDVSVDKLGEVVLQDAFQAW--------DTKQIIR-KGRERRVFLFELYLLF 1508
            ..||:|||..:...|.::..||::::|.:|..|        ..|::.| |..:|.:||.|..:||
  Rat   877 AVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHLFLHEKAVLF 941

  Fly  1509 AKEVKES-----NVVKYQFKSKLMTTDMGITEHIEGDETKFAVW-TGRSPMLSDCRIVLKATSLE 1567
            .|:.:|:     ....|.:|..|..|.:||||:::||..||.:| ..|..:     .:::|.:.|
  Rat   942 CKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEIWYNAREEV-----YIIQAPTPE 1001

  Fly  1568 TKQIWVKKLREVMQ---ETCFSGT---------SLTLP----KSPAKHSGSSQRSSRDLDEQLTE 1616
            .|..||.::|:|:.   :.|...:         ||.||    .||.|   .|.|:.:.|:::.|:
  Rat  1002 IKAAWVNEIRKVLTSQLQACREASQHRALEQSHSLPLPTPASTSPTK---GSTRNVKKLEDRKTD 1063

  Fly  1617 NDHDRCSLASFGSGNTTDSDNKLGNQEATWVVADYIATSGSNELSVSKGQQVEIVEPPTAGEPDF 1681
                                                                           ..
  Rat  1064 ---------------------------------------------------------------PL 1065

  Fly  1682 CLVRLNPQHDDAAVQEGLVPVSVLKPPPGSHKHGSGTAANAASSAGSQKSDMQDQSNRSKADALS 1746
            ||             ||.|..|:.|||                             .:.|.||:.
  Rat  1066 CL-------------EGCVSSSLPKPP-----------------------------EKGKDDAVP 1088

  Fly  1747 SST------KRRGFSGRNWLPLMNRKGTDKPPSSKPLVKKPSEKNLRTP--------QKHAEELA 1797
            |||      .|:.|:.:....|..:|.:...|..|  .|:...|:..||        ..|:.|..
  Rat  1089 SSTSESSALSRKRFTLQGLANLKGQKASPTSPDKK--AKRHEVKSDPTPFGLRGWSKTSHSLEAP 1151

  Fly  1798 EQQNQQPGASPATVLPSSQFPSSTQHPGGAGAGDYEP-------DEEVGLELPPPMKPIQEPHLI 1855
            |:..   |.|.|..|.:|   |..:..||.|.....|       |.|.|......|:......::
  Rat  1152 EEDG---GWSSAEELINS---SDAEEDGGVGPRKLVPGKYTVLMDGEKGGSDTLAMRSGDMVEVV 1210

  Fly  1856 ANGPPA--FAKDAKESSGNMASPGKMDGNPLSEIEEIV-KTTTEQHESNSRVDGGGGV-ENASTN 1916
            ..|...  :.:|...|        |....|.|.:..:: |:::.|..|:|....|..| .|:|:.
  Rat  1211 EEGTEGLWYVRDLTSS--------KEGWVPASSLATLLGKSSSAQCLSSSDSSPGSAVLSNSSSC 1267

  Fly  1917 GHG 1919
            ..|
  Rat  1268 SEG 1270

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
trio	NP_651960.2	SEC14	13..152	CDD:214706
		SPEC	314..>468	CDD:238103
		SPEC	563..778	CDD:238103	38/251 (15%)
		SPEC	782..997	CDD:238103	50/223 (22%)
		SPEC	897..1119	CDD:238103	44/222 (20%)
		SPEC	1125..1226	CDD:197544	20/101 (20%)
		RhoGEF	1287..1456	CDD:279015	75/178 (42%)
		PH1_Kalirin_Trio_like	1462..1584	CDD:270060	38/139 (27%)
		PH	1495..1580	CDD:278594	28/90 (31%)
		SH3_Kalirin_1	1643..1707	CDD:212786	6/63 (10%)
		RhoGEF	1945..2122	CDD:279015
		PH2_Kalirin_Trio_p63RhoGEF	2130..2260	CDD:270061
Mcf2l	XP_006253478.1	SEC14	145..290	CDD:214706	30/193 (16%)
		SPEC	421..623	CDD:238103	62/304 (20%)
		RhoGEF	702..879	CDD:238091	75/180 (42%)
		PH_Dbs	886..1017	CDD:269934	38/135 (28%)
		PH	900..1014	CDD:278594	34/118 (29%)
		SH3_DBS	1184..1236	CDD:212791	10/59 (17%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG4240
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	3	2.810