Protein Alignment trio and Mcf2

DIOPT Version :10

• Sequence 1: NP_651960.2
• Gene: trio / 43974
• FlyBaseID: FBgn0024277
• Length: 2263
• Species: Drosophila melanogaster
• Sequence 2: XP_006527813.1
• Gene: Mcf2 / 109904
• MGIID: 96932
• Length: 1127
• Species: Mus musculus
• Alignment Length: 955
• Identity: 232/955 - (24%)
• Similarity: 398/955 - (41%)
• Gaps: 217/955 - (22%)

  Fly   679 TAWIDKLRTDVSTDETRLSQENLEGIERILQQYQRDQQESSVNVCLTTISQGEALLQEMRSLEYA 743
            |:::.:..||:.   .|.|||              |.......|.|:::|.         .|.|.
Mouse   216 TSFLQRTFTDIG---FRFSQE--------------DFMLKLPVVMLSSVSD---------LLTYI 254

  Fly   744 DNTGSIAALEATLEKLNKQKVELEELWSARKFRADLILR--LRYFERDARLLSVQMEMWSEELQH 806
            |:......|..||:..:.:       |        :|.|  :..|....:.::..::.:..||..
Mouse   255 DDKQLTPELGGTLQYCHSE-------W--------IIFRNAIEKFAVTVKEMAQMLQSFGTELAE 304

  Fly   807 ADLSRDYQKAEQLIRMHNESVTEIQNATYEVVQQGQDLLQLFENAGFISMADATHTAQAR----- 866
            .:|.:|....|:::....|....::|....|.::|:.||...:    :...:.|.::...
Mouse   305 TELPQDILSIEEILAGRAERYHLLKNDLTAVTKEGKVLLMNLQ----VPATEETVSSSLECTQHI 365

  Fly   867 ------IEYLLDFLREREIDLEEMSEAKRAKLEQAVQLCQFQNDANQVISWIRNGEAML----VA 921
                  |:.||..:.:.|...:...|..:.|:||.:||.:|:.|..:.::.:   |.:|    ..
Mouse   366 NGDWQTIKKLLAQVHDMETAFDGFWEKHQLKMEQYLQLWKFEQDFQEAVTQV---EFLLSQQREL 427

  Fly   922 SFVTPNSLQEAEQLRKGHEQFQIAIEKTH---TSAVQVKYRADALINANHYDPLSIDEISDDVTK 983
            ..:|.|..|..::|:|    .:|..:|:.   |.|..|..|...|.:.:||   ::|.|.....:
Mouse   428 GDITGNLAQVKQRLKK----LEILDDKSQELLTKARIVILRGHKLASNHHY---ALDLICQRCNE 485

  Fly   984 -KWQQLVTYAEERHKLVTASINFYKTAEQVCSVLDSLEREYKREDDWCDGG------GGSDKAQA 1041
             ::...:...|.|.|.|..|..|            .:.|..::....||.|      .|.||.|
Mouse   486 LRYLSDILVNEIRTKRVQLSRTF------------KVHRLLQQARQCCDQGECLLASQGMDKFQ- 537

  Fly  1042 IVQLISKHQEQKEAFLKACTLARRTAETFLKYA----NRSQQCYQYKGNCEGHVKSKLDKLLTQE 1102
                 :|...||         |.:..:.||:.|    |...:..||          :.|.||:.|
Mouse   538 -----TKEDAQK---------ALQDVDNFLQMAMPFINYDIESLQY----------EFDVLLSPE 578

  Fly  1103 NQVLDYWTLRKKSLDQCQQFVLFERSAKQAIEWIHNTGEAYLSSRSNLVGISKEETEGLLKEHNE 1167
            .:.            |.|...|          .:.|...|:.:.::....: ||..||..:  |.
Mouse   579 LKA------------QMQNIQL----------KLENIRSAFQNQQAGCKSL-KEVPEGAFQ--NL 618

  Fly  1168 FRSTAKETRERVKLLIQLADSLVEKGHAHASDIKQWVASVDQRYKDFSNRMDSYCEQLEKSLGMS 1232
            ..::....|.|   :|..:...|..|::.....:.:: .|.:.::....:.:...|.:|
Mouse   619 VPASENVMRSR---MIFFSPKHVGIGYSFFQACRIFL-KVKKSWRQIHAQSNVKVEAVE------ 673

  Fly  1233 QQQLLLGSDGNSSISSSSGDRHSD--PTLEAKLNSSNKENKEINEEKRKSARRKEFIMAELMQTE 1295
                            .|.:::||  |.|:..|:.                 .|..::.||:|||
Mouse   674 ----------------DSQEKNSDQSPKLDNSLDI-----------------LKNHVLNELIQTE 705

  Fly  1296 RAYVNDLATCIKCFLEEF---RAGKSVPSALIGQEDVIFGNIKEIHHFHQKIFLRELEKYETMPE 1357
            ||||.:|.|.:..:..|.   :....:|..|..::||:|||:.||:.||..||:..||.....||
Mouse   706 RAYVRELFTVLLGYRSEMDNPQMFDLMPPLLRNKKDVLFGNMAEIYEFHNNIFMSRLEDCSDAPE 770

  Fly  1358 DVGHCFVTWASKFDMYVHYCKNKPTSNNLLVQHGG-SFFEELQRRLEVDHPLPAYLIKPVQRITK 1421
            .||.||:.....|.||..||:|||.|..:..::.. :||:|.||:|:....|.:||:||||||||
Mouse   771 RVGPCFLERKDDFQMYAKYCQNKPRSELIWRKYSECAFFQECQRKLKHRLGLDSYLLKPVQRITK 835

  Fly  1422 YQLLLKDLLSCCEESHG--EIKEGLEVMLNVPKKANDAMHLSLLENCDVSVDKLGEVVLQDAFQA 1484
            ||||||:||...:|..|  ::||.|:.||.:.|..||:||.:.:.....::::||::|||.:|..
Mouse   836 YQLLLKELLKYSKEGEGTTKLKEALDSMLELLKSVNDSMHQTAINGYVGNINELGKMVLQGSFNV 900

  Fly  1485 W--------DTKQIIR-KGRERRVFLFELYLLFAKEVKES-----NVVKYQFKSKLMTTDMGITE 1535
            |        ..|...| |..:|.:||:|..::|.|...||     ....|.||..|...|:||||
Mouse   901 WLGHRKGATKMKDFARFKPMQRHLFLYEKAVMFCKRRFESGEGADRYPSYSFKHCLKMEDVGITE 965

  Fly  1536 HIEGDETKFAVWTGRSPMLSDCRIVLKATSLETKQIWVKKLREVM 1580
            |::||..||.:.......:    .:::|.:::.|.:|:|::|:::
Mouse   966 HVKGDNRKFEIRYSEKEEI----YIVQAPNVDVKMLWLKEIRKIL 1006

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
trio	NP_651960.2	SEC14	13..152	CDD:214706
		SPEC	314..>468	CDD:238103
		SPEC	563..778	CDD:238103	18/98 (18%)
		SPEC	782..997	CDD:238103	46/235 (20%)
		SPEC	897..1119	CDD:238103	50/239 (21%)
		SPEC	1125..1226	CDD:197544	14/100 (14%)
		RhoGEF	1287..1455	CDD:459876	75/173 (43%)
		PH1_Kalirin_Trio_like	1462..1584	CDD:270060	37/133 (28%)
		SH3_Kalirin_1	1643..1707	CDD:212786
		RhoGEF	1945..2121	CDD:459876
		PH2_Kalirin_Trio_p63RhoGEF	2130..2260	CDD:270061
Mcf2	XP_006527813.1	SEC14	128..268	CDD:214706	16/77 (21%)
		SPEC	408..599	CDD:413338	52/259 (20%)
		RhoGEF	694..871	CDD:238091	76/176 (43%)
		PH-like	878..1009	CDD:473070	37/133 (28%)