Protein Alignment trio and quoa

DIOPT Version :10

• Sequence 1: NP_651960.2
• Gene: trio / 43974
• FlyBaseID: FBgn0024277
• Length: 2263
• Species: Drosophila melanogaster
• Sequence 2: XP_009295405.2
• Gene: quoa / 100148904
• ZFINID: ZDB-GENE-090313-319
• Length: 1588
• Species: Danio rerio
• Alignment Length: 1699
• Identity: 351/1699 - (20%)
• Similarity: 567/1699 - (33%)
• Gaps: 571/1699 - (33%)

  Fly   348 SVAKSLQDEHQKFAVASMNVSVNID-RI------LAVAARLIESQHYAAQHIKTLAQRLDR---- 401
            |.:.|.|.|...........|.:|: ||      :||:.||::|...      |....:|:
Zfish   194 SASPSNQQESSSLCRPPTPSSFSIETRICPARDGIAVSLRLVDSSSI------TKHSEMDQSGMR 252

  Fly   402 --------TWKDFAAGLDERTAVLQLSVL-----FHHKAEQYCNSVASWAAACQASQPLPSDIQS 453
                    ||       |.|:....:.:.     |..:.:.|.|   :|....:::    ...:.
Zfish   253 PIGWVSPSTW-------DSRSKSSLVGICNDPLDFTKETQGYAN---TWTPLLRSN----GATRE 303

  Fly   454 LETAIRTHQSLYE--AMCQAYTEVHSTSKKLLYQLDHLVQVCNQPPPPGVPDHRKNSNNGSFNKY 516
            ..|.:||.:...|  ..|......|.|.:|..: :|..:      .|....||.        |:.
Zfish   304 RSTCVRTVRFAEEPCTPCMQRKHGHGTKQKCRF-MDTAI------TPKEKSDHP--------NEA 353

  Fly   517 ERQNPAAD------YSEGASHVLAVIHQILSHHRTLEAKWHQERL--------RLH-------QT 560
            |..:|..:      |.:|.:.  ||:......|..::.:.:||..        |||       .|
Zfish   354 ETADPCGEEQIQSAYRDGLAP--AVVGTSEKCHIVIQGQSNQEDRSSYFEMIPRLHVVPGKKTTT 416

  Fly   561 LAL--------RLFQEDVKQ--------VLDWLKNHGEVFLRKNT-------------GIGRNFH 596
            ..|        ||..:|:.|        |.....||.:......|             ......|
Zfish   417 FGLVSPKLNRRRLVIKDLPQDPKSQCAPVPGSRINHPDGLTPAETERRPSRSSTPPTLKADALLH 481

  Fly   597 KARVYQT-SHDNFENVAQNTYSNAQ-------------KLLAAADELARSG-------------- 633
            ...|..| ..|..:......|.:.|             |||.....:.|.|
Zfish   482 AGIVCLTGGQDRSDRAVLEVYGDHQVWISATLSSQEIFKLLLYFHTITRKGMKDVGMTVVFDARK 546

  Fly   634 ---------------EADPNEIYSVAREL--------ELQVGSFAERVEQRR--------RRLDM 667
                           |.||..::.|...|        |.:.|...|.|...:        .:|..
Zfish   547 KPPHPEFTKALLTLHEKDPGAVHCVVLLLNKDSSYSHEKRPGVTVETVTSYKALYKLVEVSQLTA 611

  Fly   668 AV---IFYTHEKDVTAWI---DKLRTDVS--TDETRL---SQENLEGIERI-----LQQYQRDQQ 716
            |:   :.|:|    ..||   .||...||  .|.:.|   :.:.|||:.:|     :||..::|.
Zfish   612 ALHGSLLYSH----WDWIQIHQKLYPFVSDLQDASALLSGAIKKLEGVHKIDTVQDVQQCIKEQA 672

  Fly   717 ESSVNVC----LTTISQ-GEALLQEMR------------SLEYADN-TGSIAALEATLEKL-NKQ 762
            ....||.    |..:.: |.|:|..:|            |.|..|. |.....:|..:..| .|.
Zfish   673 ALMKNVLEDARLVELQRGGGAMLARLRRETDVRSPHCEQSREVIDTVTHLYNGMEEQVHVLARKS 737

  Fly   763 KVELEELWSARKFRADLILRLRYFERDARLLSVQMEMW------------------SEELQH--- 806
            .:.|:.|        |.:|:||:.|  ||.  .|::.|                  ||::|.
Zfish   738 NMSLQHL--------DFLLQLRHME--ARF--AQIKEWFDTEGETQLLQAESVDESSEQIQQTLQ 790

  Fly   807 ---ADLSRDYQKAEQLIRMHNESVTEIQNATYEVVQQGQDLLQLFENAGFISMADATHTAQARIE 868
               |.||...::..|.:.:.::: ..||...|...:....::.:|:::
Zfish   791 TFKAFLSEANERKHQAMMLVSDA-ERIQGPNYPETEVFHRMVSMFKSS----------------- 837

  Fly   869 YLLDFLREREIDLEEMSEAKRAKLEQAVQLCQFQNDANQVISWIRNGEAMLV---ASFVTPNSLQ 930
             :.||:...|...||        |...|.:|.....|:.|.     |:..|.   .|...|...|
Zfish   838 -VSDFVSRAERCCEE--------LAMMVYICHICERASAVA-----GDCRLCLEQESLCCPADEQ 888

  Fly   931 EAEQLRKGHEQFQIAIEKTHTSAVQVKYRADALINANHYDPLSIDEISDDVTKKWQQLVTYAEER 995
            ...:|   |...|...|........|:.:|.||             .|....:.|.......||.
Zfish   889 HWARL---HAHLQRLDEFPPELLQDVRTQACAL-------------SSSRALRVWDAAWVKCEEV 937

  Fly   996 HKLVTASINFYKTAEQVCSVLDSLEREYKREDDW----CDGGGGSDKAQAIVQLISKHQEQKEAF 1056
            .:::          |:...:|:..:..:....||    ||.....|             |::|..
Zfish   938 RRML----------EERLMLLEGDQTHHGHHTDWTTGQCDATALED-------------EERECV 979

  Fly  1057 LKACTLARRTAETFLKYANRSQQCYQYKGNCEGHVKSKLDKLLTQENQVLDYWTLRKKSLDQCQQ 1121
            .::.|.|.|:.::.    :.:..|:.::.      |.:.|...|.        |.|:...|..|:
Zfish   980 PESETRANRSRDSI----HNTVTCFNFRS------KPRRDSKNTA--------TTRETPADCSQR 1026

  Fly  1122 FVLFERSA---KQAI-------EWI--HNTGEAYLSSRSNLVGISKEETEGLLKEHNEFRSTAKE 1174
            .....|.|   ::|:       :|.  .||.....|.||.|     ..||. ::.||.|.|...:
Zfish  1027 GRSSSRRAAPEQRAVSGAPVGCQWFPWQNTVNRSSSRRSEL-----HITEP-IQRHNSFSSQTHD 1085

  Fly  1175 TRERVKLLIQLADSLVEKGHAHASDIKQWVASVDQRYKDFSNRMDSYCEQLEKSLGMSQQQLLLG 1239
            |                  |..|     |                                   .
Zfish  1086 T------------------HGDA-----W-----------------------------------R 1092

  Fly  1240 SDGNSSISSSSGDRHSDPTLEAKLNSSNKENKEINEEKRKSARRKEFIMAELMQTERAYVNDLAT 1304
            ::|     .::.|.|..|:...||                     :.||.||:.||..||..|..
Zfish  1093 NEG-----ENTADTHESPSRSMKL---------------------QRIMEELLLTEVEYVRSLGY 1131

  Fly  1305 CIKCFLEEFRAGKSVPSALIGQEDVIFGNIKEIHHFHQKIFLRELEKYETMPEDVGHCFVTWASK 1369
            .:..:. ...:...||..|.||...||||:::::.||.:.|.:||:..:..|...|.||:.....
Zfish  1132 ILTHYY-PLLSRPDVPQDLRGQRGRIFGNLEKLYDFHCQHFQQELQACQAEPLRAGRCFLNHRES 1195

  Fly  1370 FDMYVHYCKNKPTSNNLLVQHGGSFFEELQRRLEVDHPLPAYLIKPVQRITKYQLLLKDLLSCCE 1434
            |.:|..|.||||.|:.|:..|  .:|:..|..|.....|.:||:||||||:||.|||:::|..|.
Zfish  1196 FGLYALYSKNKPQSDALIQHH--RYFKRKQLELGDSMDLSSYLLKPVQRISKYSLLLQEILDECV 1258

  Fly  1435 ESHG----EIKEGLEVMLNVPKKANDAMHLSLLENCDVSVDKLGEVVLQDAFQAWDTKQIIRKGR 1495
            ....    ||:..|||:....:..||.:.:..:.:||:::::.|:::.||.|  |   .|.||.|
Zfish  1259 SDQSPEREEIQAALEVVRFQLRHGNDLLTMDAIRDCDLNLNEQGQLIRQDEF--W---VIFRKKR 1318

  Fly  1496 E-RRVFLFELYLLFAKEVKE---SNVVKYQFKSKLMTTDMGITEHIEGDETKFAVWTGRSPMLSD 1556
            . ||||||:..:||.|..|.   .:|  |.:|..:.|.::|:|:........|.:|..|  ..|.
Zfish  1319 SLRRVFLFQDVILFTKTKKNDRGDDV--YVYKLSIKTCEIGMTQSCGLSGRSFEIWFRR--RRSQ 1379

  Fly  1557 CRIVLKATSLETKQIWVKKLREVM------------QETCFSGTSL-----TLPKSPA-KHSGSS 1603
            ...:|:|.:.:.|..|...|..::            ||..|.||..     ..|::.| .|:.:.
Zfish  1380 DTYILQAETRDIKHAWTTDLEHILWEQALKSRELRRQERLFMGTGCKPFVDIQPRNAAVNHTVNG 1444

  Fly  1604 QRSSRDL----------------DEQLTENDHDRCSLASFGSGNTTDSDNKLGNQEATWVVADYI 1652
            ..:.|:|                ...|...|       |.|||:||.:   .|:|.::      .
Zfish  1445 DVTGRELVGILKTPNPLVSGLQRGGALPRPD-------SIGSGSTTST---AGSQSSS------S 1493

  Fly  1653 ATSGSNELSVSKGQQVEIVEPPTAGEPDFCLVRLNPQHDDAAVQEGLVPVSVLKPPPGSHKHGSG 1717
            :..||...||.:|:...:||                       ..||...:       .|...:|
Zfish  1494 SGRGSMSPSVFQGKYESLVE-----------------------NAGLTGCA-------DHTCSTG 1528

  Fly  1718 TAANAASSAGSQKSDMQDQSNRSKADALSSSTKRRGFSGRNWLPLMNRK---------GTDKPPS 1773
            ...::.||.      :..:|:.|.|.::..|:           |.::||         ||.|...
Zfish  1529 RRRSSVSSL------LHSRSSASPAHSIHYSS-----------PALSRKMLSPQTEAHGTLKCQF 1576

  Fly  1774 SKPLVKKPS 1782
            .:|:..|.|
Zfish  1577 EEPIRSKAS 1585

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
trio	NP_651960.2	SEC14	13..152	CDD:214706
		SPEC	314..>468	CDD:238103	27/145 (19%)
		SPEC	563..778	CDD:238103	64/337 (19%)
		SPEC	782..997	CDD:238103	45/241 (19%)
		SPEC	897..1119	CDD:238103	39/228 (17%)
		SPEC	1125..1226	CDD:197544	20/112 (18%)
		RhoGEF	1287..1455	CDD:459876	61/171 (36%)
		PH1_Kalirin_Trio_like	1462..1584	CDD:270060	38/137 (28%)
		SH3_Kalirin_1	1643..1707	CDD:212786	9/63 (14%)
		RhoGEF	1945..2121	CDD:459876
		PH2_Kalirin_Trio_p63RhoGEF	2130..2260	CDD:270061
quoa	XP_009295405.2	SPEC	634..>803	CDD:413338	42/180 (23%)
		RhoGEF	1114..1276	CDD:459876	61/164 (37%)
		PH_puratrophin-1	1276..1411	CDD:270062	38/143 (27%)