Protein Alignment trio and mcf2la

DIOPT Version :10

• Sequence 1: NP_651960.2
• Gene: trio / 43974
• FlyBaseID: FBgn0024277
• Length: 2263
• Species: Drosophila melanogaster
• Sequence 2: XP_073810125.1
• Gene: mcf2la / 100148439
• ZFINID: ZDB-GENE-090312-122
• Length: 1242
• Species: Danio rerio
• Alignment Length: 2018
• Identity: 366/2018 - (18%)
• Similarity: 612/2018 - (30%)
• Gaps: 934/2018 - (46%)

  Fly     6 ARDLLTLLQERVVFLTGGRDRRGGPLLCFPATPRRDRLKPEDLRRLLSYLISIPSDAAKNLGFTV 70
            |.|:...|:::..||:|||.:.|.|::.||..|....|:.::...:|:||.||||.::..:||.:
Zfish    67 AADISPDLRKQFAFLSGGRGQNGSPIIIFPEYPEFGELEEQEFHNVLTYLTSIPSLSSTGIGFIL 131

  Fly    71 IIDMRGNGNCSTNVKTILKVLQEHFSANIHNVVIIKPDNFWQKQRASI----SSHKYKFETTTV- 130
            :||.|.:...|  ||..|..:...|..|:..|::::|...:|:..:.|    :...:|.:...:
Zfish   132 VIDRRQDRWAS--VKGTLLRIAGSFPGNLQLVLVLRPTTLFQRTISDILFKFNKDDFKMKVPVIM 194

  Fly   131 --SIESLNKIVESHQLTGDFEGQQLYDHQQWTDARLAIEDFFWQAGDMADRIDDLQEDLNRNDFA 193
              |:..|:..::..|||.:..|.|.|.|::|...|.|||.|.......|..:.....:|...:..
Zfish   195 LSSVTELHSYIDRSQLTQELGGTQEYCHEKWISHRTAIEGFALMVKKTAQTLQSFGTELAETELP 259

  Fly   194 EDVPLARHAIDHHNEMRKKITKLPIEDLDM---QGKKLLAKINAMAPPGGQAPTNSGGPDMDSGP 255
            .||....:.:..|.|.:.|:.    |||.:   ||.:||..||
Zfish   260 NDVEATSNLLTVHTEKKDKMK----EDLRIALCQGGRLLESIN---------------------- 298

  Fly   256 SSAGQQSQPSQQSRSVGGNPDMS---------AAVNKALRQIELIHTGQEKLLMLWQHKKVKLDQ 311
                   :|..:      :||.|         |.|.:.|.|::...|..:.   .|...:.||:|
Zfish   299 -------EPLVK------DPDYSMNHDELENLATVQRLLGQLDETETAFDD---FWDKHQTKLEQ 347

  Fly   312 CFQWRLFEQDCEKM---FDWILHNRDVFQMSYVEIGHNYSVAKSLQDEHQKFAVASMNVSVNIDR 373
            |.|.|.|||:..::   .|.:......|....:...|...:.|.|.:..::       .|..:|:
Zfish   348 CLQLRHFEQNFREVRAHLDLVYDRLSGFSEVGISPAHAEHILKELTNHEER-------ASEVMDQ 405

  Fly   374 ILAVAA---RLIESQHYAAQHIKTLAQRLDRTWKDFAAGLDERTAVLQLSVLFHHKAEQYCNSVA 435
            .|::|:   :|||:.|||..                             |:|
Zfish   406 ALSLASDADQLIEASHYAVD-----------------------------SIL------------- 428

  Fly   436 SWAAACQASQPLPSDIQSLETAIRTHQSLYEAMCQAYTEVHSTSKKLLYQLDHLVQVCNQPPPPG 500
                      |..|:::|              :|:..:.:....|..|:
Zfish   429 ----------PKCSELRS--------------VCEEISTILRAKKAYLF---------------- 453

  Fly   501 VPDHRKNSNNGSFNKYERQNPAADYSEGASHVLAVIHQILSHHRTLEAKWHQERLRLHQTLALRL 565
                                                                :.:.|||.|
Zfish   454 ----------------------------------------------------KSVELHQCL---- 462

  Fly   566 FQEDVKQVLDWLKNHGEVFLRKNTGIGRNFHKARVYQTSHDNFENVAQNTYSNAQKLLAAADELA 630
                 ::...|                                       |.:...|||:..
Zfish   463 -----EKATKW---------------------------------------YDDGIYLLASQP--- 480

  Fly   631 RSGEADPNEIYSVARELELQVGSFAERVEQRRRRLDMAVIFYTHEKDVTAWIDKLRTDVSTDETR 695
                                                               :||.::.
Zfish   481 ---------------------------------------------------VDKCQSQ------- 487

  Fly   696 LSQENLEGIERILQQYQRDQQESSVNVCLTTISQGEALLQEMRSLEYADNTGSIAALEATLEKLN 760
                  :|.|..||:.:|                            |.|..             |
Zfish   488 ------DGAESALQEIER----------------------------YLDTA-------------N 505

  Fly   761 KQKV-ELEELWSARKFRADLILRLRYFERDARLLSVQMEMWSEELQHADLSRDYQKAEQLIRMHN 824
            :.|: :|..:|                                        |||
Zfish   506 QHKLTDLNGIW----------------------------------------RDY----------- 519

  Fly   825 ESVTEIQNATYEVVQQGQDLLQLFENAGFISMADATHTAQARIEYLLDFLREREIDLEEMSEAKR 889
            :||                |.|.|.:.                   :|.:.::::.::||.|.:|
Zfish   520 DSV----------------LTQDFRDQ-------------------VDKVFQKQLSMQEMFEKRR 549

  Fly   890 AKLEQAVQLCQFQNDANQVISWIRNGEAMLVASFVTPNSLQEAEQLRKGHEQFQIAIEKTHTSAV 954
            ..|::..                                   |:|                |..|
Zfish   550 VSLKKLA-----------------------------------AKQ----------------TRPV 563

  Fly   955 Q-VKYRADALINANHYDPLSIDEISDDVTKKWQQLVTYAEERHKLVTASINFYKTAEQVCSVLDS 1018
            | |..|.:|:|..    |:|                                           ..
Zfish   564 QPVAPRPEAIIKT----PVS-------------------------------------------SP 581

  Fly  1019 LEREYKREDDWCDGGGGSDKAQAIVQLISKHQEQKEAFLKACTLARRTAETFLKYANRSQQCYQY 1083
            ..||...|.|                                                     ..
Zfish   582 AHREKSIEGD-----------------------------------------------------VI 593

  Fly  1084 KGNCEGHVKSKLDKLLTQENQVLDYWTLRKKSLDQCQQFVLFERSAKQAIEWIHNTGEAYLSSRS 1148
            .|||
Zfish   594 NGNC------------------------------------------------------------- 597

  Fly  1149 NLVGISKEETEGLLKEHNEFRSTAKETRERVKLLIQLADSLVEKGHAHASDIKQWVASVDQRYKD 1213
                                                      :||..|
Zfish   598 ------------------------------------------KKGELH----------------- 603

  Fly  1214 FSNRMDSYCEQLEKSLGMSQQQLLLGSDGNSSISSSSGDRHSDPTLEAKLNSSNKENKEINEEKR 1278
                                    |.::|:|        ||                ..:::|:.
Zfish   604 ------------------------LQNEGSS--------RH----------------PSVSDEEE 620

  Fly  1279 KSARRKEFIMAELMQTERAYVNDLATCIKCFLEEFRA-------GKSVPSALIGQEDVIFGNIKE 1336
            ..|..:..:|.||::||||||.:|.    |.||.:.|       ...:|:.|:.::|::|||:.|
Zfish   621 NFAVLRRHVMNELLETERAYVEELL----CVLEGYAAEMDNPAMSHLIPNTLLHKKDILFGNMSE 681

  Fly  1337 IHHFHQKIFLRELEKYETMPEDVGHCFVTWASKFDMYVHYCKNKPTSNNLLVQHGG-SFFEELQR 1400
            |:.||:|.||||||.|...||.||.||:...:...:|..||:|||.|.:|..|... :||:|.|:
Zfish   682 IYQFHKKTFLRELEAYTDYPELVGQCFLERMTDLQIYEKYCQNKPRSESLWRQCSDCAFFQECQK 746

  Fly  1401 RLEVDHPLPAYLIKPVQRITKYQLLLKDLLSCCEESHGE--IKEGLEVMLNVPKKANDAMHLSLL 1463
            :||....|.:||:||||||||||||||:||...:...||  ::|.|..:|.:.|..||:|||..:
Zfish   747 KLEHKLGLDSYLLKPVQRITKYQLLLKELLKYSKGCEGEEDLQEALSSILGILKAVNDSMHLIAI 811

  Fly  1464 ENCDVSVDKLGEVVLQDAFQAW--------DTKQIIR-KGRERRVFLFELYLLFAKEVKES---- 1515
            ...:.::..||.:::|.:|..|        ..|.:.| |..:|.:||.|..|||.|:.:|:
Zfish   812 TGYEGNLSDLGRLMMQGSFSVWTEHKKGHAKVKDLARFKPMQRHLFLHEKALLFCKKREENGEGY 876

  Fly  1516 -NVVKYQFKSKLMTTDMGITEHIEGDETKFAVWTGRSPMLSDCRIVLKATSLETKQIWVKKLREV 1579
             ....|.||..|..:.:||||:.:||..||.:|......:    .:::|::.|.|..||.::|:|
Zfish   877 EKAPSYSFKHSLSMSAVGITENAKGDNKKFEIWCNSREEV----YIVQASTPEIKTAWVNEIRKV 937

  Fly  1580 MQETCFSGTSLTLPKSPAKHSGSSQRSSRDLDEQLTENDHDRCSLASFGSGNTTDSDNKLGNQEA 1644
            :..           :..|....|.|:||            |:.|..:..|...|.|..|
Zfish   938 LTR-----------QLKACREASQQKSS------------DQISPLTPSSTGLTLSPFK------ 973

  Fly  1645 TWVVADYIATSGSNELSVSKGQQVEIVEPPTAGEPDFCLVRLNPQHDDAAVQEGLVPVSVLKPPP 1709
                        :|..|:.||.:.: .||.|.......|.:.|.:..|..|      ||     |
Zfish   974 ------------TNPKSLKKGDEKK-TEPTTTDPSSAALFKNNDKLKDETV------VS-----P 1014

  Fly  1710 GSHKHGSGTAANAASSAGSQKSDMQDQSN-RSKADALSSSTKRRGFSGRN-WLPLMN--RKGTDK 1770
            .:.:          ::...::..:|..|| :|:.:.|::|      .|:: .||::.  ..|: .
Zfish  1015 NTER----------AAVAKKRFTLQGFSNLKSQKELLAAS------GGKSLTLPMVTLCHPGS-S 1062

  Fly  1771 PPSSKPLVKK---PSEKNLRTPQKH--------AEELAEQQNQQPGASPATVLPSSQFPSSTQHP 1824
            |||.....|:   |:....|....|        ....:..|.::.|.:.|    |....:|.::.
Zfish  1063 PPSPDHKTKRQSDPTPFGFRDAGPHITLTRVRWLSTSSLLQTKRRGFTKA----SLSMDASEEND 1123

  Fly  1825 GGAGAGD---YEPDEEVGLELPPPMKPIQEPH-----------------LIANGPPA--FAKDAK 1867
            |.:.|.|   .:|::|.|.:|.|....:...|                 |:..|...  |.::.:
Zfish  1124 GYSSAEDPMNSDPEDEGGKKLSPGKYVVVADHDKSGPQELTVKSGDTVQLVREGDDGRWFVRNLR 1188

  Fly  1868 ESSGNMASPGKMDGNPLSEIEEIV-KTTTEQHESNSRVDGGGGVENASTNGHGRSHDNNDESHTA 1931
            .|        |....|.:.:..:: ::.:.|..|:|...|.|.:..:|         :..|::|:
Zfish  1189 TS--------KEGWVPAANLLTLIGESKSSQSLSSSEGSGSGNLSTSS---------SCSETYTS 1236

  Fly  1932 VSD 1934
            .||
Zfish  1237 FSD 1239

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
trio	NP_651960.2	SEC14	13..152	CDD:214706	41/145 (28%)
		SPEC	314..>468	CDD:238103	25/159 (16%)
		SPEC	563..778	CDD:238103	18/215 (8%)
		SPEC	782..997	CDD:238103	25/215 (12%)
		SPEC	897..1119	CDD:238103	18/222 (8%)
		SPEC	1125..1226	CDD:197544	3/100 (3%)
		RhoGEF	1287..1455	CDD:459876	78/177 (44%)
		PH1_Kalirin_Trio_like	1462..1584	CDD:270060	37/135 (27%)
		SH3_Kalirin_1	1643..1707	CDD:212786	13/63 (21%)
		RhoGEF	1945..2121	CDD:459876
		PH2_Kalirin_Trio_p63RhoGEF	2130..2260	CDD:270061
mcf2la	XP_073810125.1	None