Protein Alignment trio and arhgef7

DIOPT Version :10

• Sequence 1: NP_651960.2
• Gene: trio / 43974
• FlyBaseID: FBgn0024277
• Length: 2263
• Species: Drosophila melanogaster
• Sequence 2: XP_012813343.1
• Gene: arhgef7 / 100145259
• XenbaseID: XB-GENE-962606
• Length: 861
• Species: Xenopus tropicalis
• Alignment Length: 1022
• Identity: 193/1022 - (18%)
• Similarity: 346/1022 - (33%)
• Gaps: 339/1022 - (33%)

  Fly  1128 SAKQAIEWIHNTGEAYLSSRSNLVGISKEETEGLLKEHNEFRSTAKETRERVKLLIQLADSLVEK 1192
            ||:|.:.|:...|  .|.|....:    .:.||.|      :::.|:.....:||.:|....::|
 Frog     3 SAEQTVTWLITLG--VLESPKKTI----SDPEGFL------QTSLKDGVVLCRLLERLLPGSIDK 55

  Fly  1193 GHAH---------------------------ASDIKQWVASVDQRYKDFSNRM------------ 1218
            .:..                           |:|:.|     .|.:....|.:
 Frog    56 VYPEPRTDTECLGNIREFLRGCSAFNLETFDANDLYQ-----GQNFNKVLNSLVLLNKVTADIGL 115

  Fly  1219 --DSYCEQLEK-------SLG----MSQQQLLLGSDGNSSISSSSGDRHSDPTLEAKLN------ 1264
              ||.|.:...       |||    :|:...||.|...|...:.:.:.|.  .:.||.|
 Frog   116 GSDSVCARSSSHRIKSFDSLGSQSVLSRSSKLLQSQYRSLDMTENSNHHM--VVRAKFNFQQTNE 178

  Fly  1265 ---SSNKEN-----------------------------KEINEEKR-----------------KS 1280
               |.||.:                             :|:...::                 .|
 Frog   179 DELSFNKGDLIHVTRLEEGGWWEGTHSGKTGWFPSNYVREVKSSEKPVSPKSTTLKSPPKGFDTS 243

  Fly  1281 ARRKEF---IMAELMQTERAYVNDLATCIKCFLEEFRAGKSVPSALIGQEDVIFGNIKEIHHFHQ 1342
            |..|.:   ::..::.||..|..:|...:..:|...:..:.:....|   ..:.||::||..| |
 Frog   244 AINKSYYNVVLQNILDTEGEYSKELQDILSNYLRHLQTSEKLSPTNI---SYLLGNLEEICAF-Q 304

  Fly  1343 KIFLRELEKYETMPED---VGHCFVTWASKF-DMYVHYCKNKPTSNNLLVQHGGSFFEELQRRLE 1403
            :|.::.||:...:||.   ||.||::...:. .:|:.||.|.|::.|:|.||.    |||...:|
 Frog   305 QILVQSLEECTKLPEGQQRVGGCFMSLMPQMKSLYIAYCANHPSAVNVLTQHS----EELGEFME 365

  Fly  1404 V---DHP----LPAYLIKPVQRITKYQLLLKDLLSCCEESH---GEIKEGLEVMLNVPKKANDAM 1458
            :   :.|    |...|.||..|:.||..|||:|....|:.|   .:|::.:....|:..:..:
 Frog   366 MKGANSPGILVLTTGLSKPFMRLDKYPTLLKELERHMEDYHPDRPDIQKSMTAFKNLSAQCQE-- 428

  Fly  1459 HLSLLENCDVSVDKLGEVVLQ---DAFQAWDTKQIIRKG------------------RERRVFLF 1502
                       |.|..|:.||   :..:.|:.:.|...|                  .||.:.||
 Frog   429 -----------VRKRKELELQILTETIRGWEGEDIKTLGTVIYMSQVMIQCAGSEEKNERYLLLF 482

  Fly  1503 -ELYLLFAKEVKESNVVKYQFKSKLMTTDMGITEHIEGDETKFAVWTGRSPMLSDCRIVLKATSL 1566
             .:.|:.:...:.|..:   ::.|:..|.|.:|: :|.:|.....:.....|:.  ||::...:.
 Frog   483 PNVLLMLSASPRMSGFI---YQGKIPLTGMCVTK-LEDNENHKNAYEIAGNMIE--RIIVSCNNQ 541

  Fly  1567 ETKQIWVKKLREVMQETCFSGTSL--------TLPKSPAKHSGSSQRSSRDLDEQLTENDHDRCS 1623
            :..|.||..|.:..:.|.....::        |||..|.  :.||:.|...| ..||...|....
 Frog   542 QDLQEWVDYLHKQTKYTSLINPTIKPHSVPCHTLPSHPV--TPSSKHSDCKL-MPLTPAYHTLPH 603

  Fly  1624 LASFGSGNTTDSDNKLGNQEATWVVADYIATSGSNELSVSKGQQVEIVEPPTAGEPDFCLVRLNP 1688
            |:..|:.::|.|.:.|                                |||...:|
 Frog   604 LSHHGTPHSTISWSPL--------------------------------EPPKTPKP--------- 627

  Fly  1689 QHDDAAVQEGLVPVSVLKPPPGSHKHGSGTAANAASSAGSQKSDMQDQSNRSKADALSSSTKRRG 1753
                       ..:|.|:|.|...          .|:|...|.|            ||.|.|
 Frog   628 -----------WSLSCLRPAPPLR----------PSAALCYKED------------LSKSPK--- 656

  Fly  1754 FSGRNWLPLMNRKGTDKP--PSSKPLVKKPSEKNLRTPQKHAEELAEQQNQQPGASPATVLPSSQ 1816
                          |.|.  |..|| .:|||::.....:..|....:.|..:...:..|...:.|
 Frog   657 --------------TMKKLLPKRKP-ERKPSDEEFALRKSTAALEEDAQILKVIEAYCTSAKTRQ 706

  Fly  1817 FPSSTQHPGGAGAGDYEPDEEVGLELPPPMKPIQEPHLIANGPPAFAKDAKESSGNMASPGKMDG 1881
            ..:||..               |.:|       ...|::|:..    :.:.:|.|..:|..:::.
 Frog   707 TLNSTWQ---------------GTDL-------MHNHVLADDD----QSSLDSLGRRSSLSRLEP 745

  Fly  1882 NPLSE---IEEIVKTTTEQHESNSRVDGGGG------------VENASTNGHGRSHDNN-DESHT 1930
            :.|||   .:.|....:.:..|.||...|..            ||...:||.....:.: .::..
 Frog   746 SDLSEDSDYDSIWTAHSYRMGSTSRCRKGSTPQVLLPEEEKIIVEETRSNGQTVIEEKSLVDTVY 810

  Fly  1931 AVSDKAAALKKRQCVFAELMSTEEAYVQDLHEIVNGYMTEINNTNSD 1977
            |:.|:...||:......:.:..|:...::|.:::...:..:|:.:.|
 Frog   811 ALKDEVQELKQDSKKMKKSLEEEQRARKELEKLIRKVLKNMNDPSWD 857

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
trio	NP_651960.2	SEC14	13..152	CDD:214706
		SPEC	314..>468	CDD:238103
		SPEC	563..778	CDD:238103
		SPEC	782..997	CDD:238103
		SPEC	897..1119	CDD:238103
		SPEC	1125..1226	CDD:197544	23/138 (17%)
		RhoGEF	1287..1455	CDD:459876	50/181 (28%)
		PH1_Kalirin_Trio_like	1462..1584	CDD:270060	27/143 (19%)
		SH3_Kalirin_1	1643..1707	CDD:212786	6/63 (10%)
		RhoGEF	1945..2121	CDD:459876	4/33 (12%)
		PH2_Kalirin_Trio_p63RhoGEF	2130..2260	CDD:270061
arhgef7	XP_012813343.1	CH_betaPIX	3..114	CDD:409115	20/127 (16%)
		RhoGEF67_u1	116..162	CDD:465201	11/45 (24%)
		SH3_betaPIX	166..219	CDD:212994	6/52 (12%)
		RhoGEF	250..427	CDD:238091	50/184 (27%)
		PH_Cool_Pix	458..556	CDD:269932	19/103 (18%)
		RhoGEF67_u2	615..706	CDD:465200	29/182 (16%)
		betaPIX_CC	777..860	CDD:465158	12/81 (15%)