Protein Alignment spag and RPAP3

DIOPT Version :10

• Sequence 1: NP_524664.1
• Gene: spag / 43958
• FlyBaseID: FBgn0015544
• Length: 534
• Species: Drosophila melanogaster
• Sequence 2: NP_078880.2
• Gene: RPAP3 / 79657
• HGNCID: 26151
• Length: 665
• Species: Homo sapiens
• Alignment Length: 680
• Identity: 167/680 - (24%)
• Similarity: 275/680 - (40%)
• Gaps: 164/680 - (24%)

  Fly     1 MSAQEKAFELQRQVRQNAREYENSVKDLYSWEQDIKNKEKELQKS---------PLSAAN----- 51
            |::..||.|||.||:|||.|.::.::||.:||:|||.|:.||::.         |:...|
Human     1 MTSANKAIELQLQVKQNAEELQDFMRDLENWEKDIKQKDMELRRQNGVPEENLPPIRNGNFRKKK 65

  Fly    52 ----KDLPVRSHVQTDKSR----------------------KESPSSSAASSPTEKQDLPVDPVA 90
                |:...::..:..|:|                      |:..:..:.|..:|.::   |.:.
Human    66 KGKAKESSKKTREENTKNRIKSYDYEAWAKLDVDRILDELDKDDSTHESLSQESESEE---DGIH 127

  Fly    91 QQYKKANDIKDRGNTYVKQGEYEKAIVAYSTAIAVYPHDPIYHINRALCYLKQESFDQCVEDCEA 155
            ...:||..:|::||.|.|||:|::||..|:..:...|::|:...|||..|.:.:.|.....||..
Human   128 VDSQKALVLKEKGNKYFKQGKYDEAIDCYTKGMDADPYNPVLPTNRASAYFRLKKFAVAESDCNL 192

  Fly   156 AIALDKLCVKAYYRRMQANESLGNNMEALKDCTTVLAIEPKNIEAKRSLARINDRLRK------- 213
            |:||::...|||.||..|..:|....||.||...||.:||.|.||...|.:|:..|..
Human   193 AVALNRSYTKAYSRRGAARFALQKLEEAKKDYERVLELEPNNFEATNELRKISQALASKENSYPK 257

  Fly   214 ---IATKSGPNFTPDRPGMIEILPIEKPA----------YKRSK--------------------- 244
               |..||    |......||....::.|          :|..|
Human   258 EADIVIKS----TEGERKQIEAQQNKQQAISEKDRGNGFFKEGKYERAIECYTRGIAADGANALL 318

  Fly   245 KAMRSVPVVDVVSPRATIDDSNQLRISDEDIDKIF------NSNCGIIEEVKKTNPKPTPMPDTS 303
            .|.|::..:.:........|..|..:.|....|.|      .:..|.:.|.|:  ...|.:....
Human   319 PANRAMAYLKIQKYEEAEKDCTQAILLDGSYSKAFARRGTARTFLGKLNEAKQ--DFETVLLLEP 381

  Fly   304 GPPKAET-IAKTSKEV------------KPTKQTAVKVAPAVETPKETETRKDTK--IVPESDN- 352
            |..:|.| ::|..||:            ...:|..||   .::.|....:.|..|  |:.|:.|
Human   382 GNKQAVTELSKIKKELIEKGHWDDVFLDSTQRQNVVK---PIDNPPHPGSTKPLKKVIIEETGNL 443

  Fly   353 --------EAKPSAPKKTAVEVPKVQTQVSPPKTTIERS-------PEVNTVQTE--KIEQASSN 400
                    ....:||:...:.:..|   ::...||.:::       |..:|.:.:  |||:.|..
Human   444 IQTIDVPDSTTAAAPENNPINLANV---IAATGTTSKKNSSQDDLFPTSDTPRAKVLKIEEVSDT 505

  Fly   401 NAMSPS---------------PIE----------RFLPPAPTSTAQFHVTWKEL-SGPQK-YQYL 438
            :::.|.               |||          ..|||.|.::.|....:::| |.|.. ||||
Human   506 SSLQPQASLKQDVCQSYSEKMPIEIEQKPAQFATTVLPPIPANSFQLESDFRQLKSSPDMLYQYL 570

  Fly   439 KSIEVPNLCKILGAGFDSDTFADLLRTIHDFFVPNKEPNTA-AVLLEISKNDEFTILAMLMSAEE 502
            |.||.....|:.....|.|.|..:::.:|||::..::|... .:|..:|:...|.:..|.||..|
Human   571 KQIEPSLYPKLFQKNLDPDVFNQIVKILHDFYIEKEKPLLIFEILQRLSELKRFDMAVMFMSETE 635

  Fly   503 KKMVSSILNAIKNWPSKNPAVLDNLFKEYG 532
            ||:..::.|.|.....|:.:| :.|.|.||
Human   636 KKIARALFNHIDKSGLKDSSV-EELKKRYG 664

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
spag	NP_524664.1	TPR repeat	96..124	CDD:276809	12/27 (44%)
		TPR	102..>211	CDD:440225	42/108 (39%)
		TPR repeat	129..159	CDD:276809	9/29 (31%)
		TPR repeat	164..192	CDD:276809	12/27 (44%)
		PLN03209	<296..416	CDD:178748	34/177 (19%)
		RPAP3_C	416..503	CDD:464012	26/89 (29%)
RPAP3	NP_078880.2	TPR 1	8..41		17/32 (53%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	37..82		6/44 (14%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	109..129		3/22 (14%)
		TPR	126..372	CDD:440225	65/251 (26%)
		TPR 2	133..166		13/32 (41%)
		TPR repeat	133..161	CDD:276809	12/27 (44%)
		TPR repeat	166..196	CDD:276809	9/29 (31%)
		TPR 3	168..200		10/31 (32%)
		TPR 4	201..234		15/32 (47%)
		TPR repeat	201..229	CDD:276809	12/27 (44%)
		TPR 5	282..315		3/32 (9%)
		TPR repeat	286..310	CDD:276809	2/23 (9%)
		TPR repeat	315..345	CDD:276809	4/29 (14%)
		TPR 6	317..349		5/31 (16%)
		TPR 7	350..383		6/34 (18%)
		TPR repeat	350..377	CDD:276809	6/28 (21%)
		RPAP3_C	546..636	CDD:464012	26/89 (29%)