Protein Alignment wb and lama4

DIOPT Version :10

• Sequence 1: NP_723870.1
• Gene: wb / 43946
• FlyBaseID: FBgn0261563
• Length: 3375
• Species: Drosophila melanogaster
• Sequence 2: XP_002936983.2
• Gene: lama4 / 100489161
• XenbaseID: XB-GENE-1004770
• Length: 1913
• Species: Xenopus tropicalis
• Alignment Length: 2769
• Identity: 557/2769 - (20%)
• Similarity: 891/2769 - (32%)
• Gaps: 1070/2769 - (38%)

  Fly   767 FLSVLVSL-------DAVLIRAAFHTD-QGETSLERAVIYSGGVELGGKSSSQVEQCLCPAGYTG 823
            |:.:|:||       ...:..:|||.| :|.||:.           .|:...|::....||....
 Frog     9 FICMLMSLWRYLCVCSGSMEGSAFHLDSEGSTSIP-----------SGQDMVQLQHVYIPARLVP 62

  Fly   824 LSCEGCAFGFKRIYENTSDHQILSKCIPCPCNGHSNSCDLQSGNCGDCMHNTFGDRCERCQLGYY 888
             ..:.|..||.        :.:..:|:||.|||::..|...||.|.||..||.|.:|:||..||.
 Frog    63 -PAQRCHMGFY--------YSVSGECLPCNCNGNAQKCLDGSGECLDCQRNTTGKQCDRCPPGYL 118

  Fly   889 GNPLQGTPNDCKRCACPLSEDSNNFSPSCQLKSYNYMDLNPQFELIEHAEYICTQCPEGYTGDHC 953
            ||.::|.|..|..|.|||....|||:.:|.:||.:...:                |.:.|.|.:|
 Frog   119 GNVVRGVPKSCLPCPCPLPFSPNNFAIACGVKSGSVRCI----------------CKDNYAGPNC 167

  Fly   954 QVCDDGYFGNPRQPGSSCQRCDCAGG--------PCNVTTGECITCRGNTEGWHCERCKLGYWGD 1010
            :.|..||:|||...||:|::|||:|.        .||..||:|..|..||.|::||||.|||:||
 Frog   168 ERCAPGYYGNPLLKGSTCKKCDCSGNSDPNLIFEDCNELTGQCNNCMRNTTGFNCERCALGYYGD 232

  Fly  1011 PAVGCDPCHCHTEGSESGLCDSTDGQCLCKPRYAGQKCDECDVGYANVELRCPSCNCDPLGSLVQ 1075
            ..|                               .:.|.||         ||..|.        .
 Frog   233 ARV-------------------------------AKNCKEC---------RCSKCG--------T 249

  Fly  1076 DRCDPHTGQCHCKEGVMGAKCHECQDGYFGMNAVAYRMDDLAALRQNSDSDDDEWELVPDTEDPN 1140
            :.|:..||.|.||.||.|..|..|:.||:|..
 Frog   250 ETCNDITGTCQCKPGVTGVFCDHCEAGYYGYT--------------------------------- 281

  Fly  1141 SESTVACEECHCSSVGSLSSDCDKRTGQCACLANVTGRRCDKCRPGHWNLTAGEGCRDCRCDPHG 1205
              |.:.|::|.||. .||.::||..|.||.|.....|.:|::|:||:||
 Frog   282 --SCLGCQKCMCSP-ASLDNNCDPETRQCNCKPGAGGLKCERCKPGYWN---------------- 327

  Fly  1206 SRGHECNPWTGQCDCKIGVGGQHCNECTEGFFGFSTEGCQRCSACRSEGQVCDPHNGRCICPKFT 1270
                                           :|.|
 Frog   328 -------------------------------YGPS------------------------------ 331

  Fly  1271 RGLGCGQCVPGTWGWQARLGCRECECDHIGSIGQQCSTGDGQCQCREGYSGRKCDTCAIGYFGYP 1335
                               ||::|:|            |||.|.
 Frog   332 -------------------GCQKCDC------------GDGPCN--------------------- 344

  Fly  1336 ECRRCGCDAEGSFTQADGSIACDSNGQCPCKSLVVGLKCDTCMQSTFGLSAQNPEGCTRCFCFGR 1400
                                                                           .:
 Frog   345 ---------------------------------------------------------------NK 346

  Fly  1401 SGECEQSDLSWGHIRMAESRNLSVQQIRPHSVPSSDYEYIVVVQMEGSSFHREDAEIQRMNDLSL 1465
            :|||...|                    |.|..|||           .||..:......::||.:
 Frog   347 TGECLLED--------------------PESPSSSD-----------CSFDCDKCIWDLIDDLRI 380

  Fly  1466 V------PKSTG-NVSIGAYGQFYHPLYFQLPPQFYGDRTSSYGGFLYFTLITEGAHKPLERNIL 1523
            .      .|:|. |:|.|.                                   .|||.|.
 Frog   381 AALLGDETKTTALNISTGV-----------------------------------AAHKHLN---- 406

  Fly  1524 GQYPLVQLHAHSKLLLDFYEYEEFEYSLNVTHRVPLHESFWKYHHTSQAVDRNTLMAALQNIRHI 1588
                    :.:|.|                            :|.|....|:|.     |:|
 Frog   407 --------YINSTL----------------------------FHLTEMLEDKNN-----QSI--- 427

  Fly  1589 FIRAFAFADFQEVVLQNVHMDAAIYIKGSTNLIAKGVERCKCPKRFDGLSCQDPGRSFYRWRNTT 1653
                     |.::::.|....... ::...|.:|.
 Frog   428 ---------FTDLMIDNAESKVEA-LQTKVNELAD------------------------------ 452

  Fly  1654 IVESVFIEDLIGRAAPCHCNGRSSDCDRETGVCQNCRGNTG---GDHCHQCAEGFYGDPNSPHGC 1715
                                                :||..   |...|:
 Frog   453 ------------------------------------KGNLAEMKGSRFHK--------------- 466

  Fly  1716 QACPCPETNRNFARGCNVWDGEVSCVCKPGYTGRLCERCQAGYFGDPMRYPNTTCQPCNCHPDGI 1780
                  ||.....|.                      :..||...|              ..|||
 Frog   467 ------ETMETMKRA----------------------KMTAGQVND--------------IEDGI 489

  Fly  1781 QTEGCDVETGRCYCREGVTGLKCDKCQAERHHLVDNGCKICDNCTLLLLDYMELVGNKLRRGMHN 1845
            |                                                   ||:|
 Frog   490 Q---------------------------------------------------ELLG--------- 494

  Fly  1846 MDLTGIPAPYRKLSEYESAYEKWNGRHWDFSQTKRRLQ-DYDSADILKLEAHAENLKFQSRKAVA 1909
                       ||..|||                  || |..:||.:|....||.:
 Frog   495 -----------KLEYYES------------------LQGDLSAADRVKSVKQAEQM--------- 521

  Fly  1910 TIGKREFAIKSMRE-DAVTQ--------QHSVGLLRSEILQTLSDLHGYGKSAHYLSLPTALKQA 1965
                    :|.|.| |..||        ::...||| |||.....:....:|.    ||..|::
 Frog   522 --------LKEMEELDFSTQRIALEEEEEYVEELLR-EILDEWQQVQNNTRSL----LPNVLEK- 572

  Fly  1966 RFYLQAIREHDQMVQGIRSTNDCAWKHFYAMGNASDASFDESGRLEMLWRDLNQT--NHRVVDMR 2028
                  |.|||                        |...|....||....|:|||  .::....:
 Frog   573 ------IAEHD------------------------DELADLYEALEEARHDINQTREKNKANIAK 607

  Fly  2029 LQVDRVQEVE--NEAEDVLEHVRNLSIRVGESH---QELDELNQRISDH---LDPGYL---EQGE 2082
            |.:..:|.|:  .|.|:|.|.:...:|.:..:|   .::.::.:.:|.|   :|..|.   |:.|
 Frog   608 LHMSNIQTVKLTKEVENVSETLTQANITLNGTHMLISDISDITKNMSGHHAEVDGAYTGLKERLE 672

  Fly  2083 GLLR-------------LTVQRQIM-LNGHLNQLDGYRILLNTTLGVKTEQQREVRKHWLPKAEK 2133
            .|.|             ||:||:.. |...|..:|
 Frog   673 NLSRNVENIVEEAVAHSLTLQREASGLTSDLKGID------------------------------ 707

  Fly  2134 HASHLLARSNEYARKFQPTRNGARIAMLASSAHS---NITEAINDARLASILAKERVYEAQRTLY 2195
                    :|.:.:|          |:.||:.|.   |:.||.|:....::...|||.:|
 Frog   708 --------ANGFVQK----------AIDASNVHESIVNMIEAANETSFIALSTAERVIDA----- 749

  Fly  2196 PSDGSSMIERAKHSLHRSKQLQQEALKQMHKSNVLKDKLHRQEQQVEGIKSTIYDSGLRTNNISG 2260
             :||  |..:.|:.:..|                  :||..|.::::|...:..|.|     ||.
 Frog   750 -TDG--MDNQIKYQMTES------------------EKLLVQAKELKGASDSSKDLG-----ISE 788

  Fly  2261 QLQGLSDGSARRQA-KDSLEMA-----DRTGEQMRAELQKAK-DMQKSIQNMRNSFSNLEPDWEI 2318
            ..|.::..:.|:.| .|.:|.|     ....|..|..|:|:| |.:::: |:....|.:......
 Frog   789 TKQHVNSATLRKDALSDKIENAISQIEVHEDENTRKRLEKSKLDAEEAL-NITAIISKVTDPMSK 852

  Fly  2319 KLGMAQENISLTQTNLRLANVSLSYLEQQAEKEQQVFEV--------------W-NNSMAQQLQQ 2368
            ...:..||:|.|..:....|   ..:....|..:.:.||              | .|:::..:.:
 Frog   853 NAKIWSENLSNTDFDASAYN---KVVNSAGEAVRNLTEVVPLLLNKLRTVDYKWPTNNISSSILR 914

  Fly  2369 LRDQIAKARHAAEAIDVSLESLGPKCIRSYLPASYGLSTSNKIRMSFALSNHLESSPLIHL---- 2429
            :|:.||:.|..|..:.||:...|...:      ...|.| |...::...|..|..:|:.|.
 Frog   915 IRELIAQTRSVASKVQVSMRFGGKSAV------DVNLKT-NAADLNAFSSISLYLNPIAHQEKPH 972

  Fly  2430 ---------ASSEGRHITLELYKRRVRLVWNLGGTTATVTHPMVVHTRDPKYDDAW--YHVEANR 2483
                     .:|...::.|.:....:..|:|||  :..|..|:     |.|..::|  |.
 Frog   973 DKFIMYFGNKNSTSDYMGLAIKSNNLVYVYNLG--SGDVEIPL-----DSKPVNSWPGYF----- 1025

  Fly  2484 TLNLGSLV-VRRMNNYGE--LTPPNPVIITGSTDTEHTRFYQ-----SRSDRISL---------- 2530
                 ||: :.|:..:|:  ||.|:|      :.|...:|.:     .|...:.|
 Frog  1026 -----SLIKIERLGRHGKVLLTVPSP------SSTAEEKFIKKGEATGRDSVLDLDPSSMVFYVG 1079

  Fly  2531 ---GGF---ASKDLQFTPGLNVVVHQVEVDNKPLGLWNF-------VTSEGSCGGSMVGAKESSA 2582
               .||   ||.||   ||....:....|::..:.|:||       ..:...|..:.:...:|.|
 Frog  1080 GVPSGFQLPASLDL---PGFVGCLELSTVNDDVVSLYNFKNIYNLNTATAPPCARNKLAFTQSRA 1141

  Fly  2583 SSTARHFNGLGYAQL--MKTRPRPTRKNLFSVQMTFRTLDENALLFLAVDDKNNRSVSVTLSRGR 2645
            :|  ..|:|.|||.:  |:.|.|.|:...|.:::  ||..:|||:||.|:  ..:..|:.|..|.
 Frog  1142 AS--YFFDGTGYAVIRNMERRGRFTQVTRFDIEV--RTPVDNALIFLMVN--GTKFFSMELQDGY 1200

  Fly  2646 IMFRIDYG---DESKLEINTTKKYNVGQWIKIEAAR--EFSAKRSTENGMLRVNNDRPISGAPTL 2705
            :....|:|   ....|| ::.||:.|..      ||  |.|........|:.|.:.|.:...
 Frog  1201 LRLHYDFGFAKGPVLLE-DSAKKFQVND------ARYHEISVIYHNSKKMILVVDRRHVKSV--- 1255

  Fly  2706 PVNIHLLPDLSKTV-----YYLGGVPPGFTSGTSKAPGADNPFLGCMKDVQVNGETYDPLESSSY 2765
                    |..||.     .|:||.|...........|||..|.||:|..|...:.::.||....
 Frog  1256 --------DNEKTSIPFSDIYIGGAPSLVLQSVKSHLGADIAFKGCIKGFQFQKKDFNLLEEPDT 1312

  Fly  2766 YGVEPSCKN---MITKAGFSGNGYLELPSQSLRKRSN--TALVFRTLQPDCLLLLAAYPPEILGD 2825
            .|:...|..   |..:|.|:|..:: ..||.|....:  ....|:||||..|||..:
 Frog  1313 LGISYGCPEESLMSRRAYFNGQSFI-ASSQKLAPFDSFEGGFSFKTLQPSGLLLYHS-------- 1368

  Fly  2826 YDAKDIKGNFSISLVDGQLHVWVNSGRSFIKMSSNSSQMNDGEFHVVHLIKTGRKLELMVDDELQ 2890
             :..|:   ||:|:..|.:.:.:..    ||:.|.:.:.:||:.|.|....:..:.:|:||:...
 Frog  1369 -EGADV---FSVSMDKGSVVLRIKD----IKVQSKNRKYSDGQSHFVVASVSPTRYQLLVDETDA 1425

  Fly  2891 EIRNLNGNPTVVSLPRDAGGLYIGGAPPHESYTPLAPTFVNLEGAIRDVVFN--NRTINFNDALT 2953
            .::: ...|...||.  |...|.||       ||:..:..|..|.|.:..|.  :|.:...|...
 Frog  1426 SVQD-RQKPGASSLV--ARTFYFGG-------TPVGISQANFTGCISNAYFTRVDRDVEVEDFQK 1480

  Fly  2954 FA-NVQ---------------IGRNGPLMGSLKGGL-----YDVLLKTEPMIGKSFTASPEGCKR 2997
            :. .||               ..:||......||.|     .|..|::....|...|........
 Frog  1481 YTEKVQSSLYGCPVESPPVALFQKNGKNSSKDKGKLRKSVGMDKTLESHAAFGSKETEEDSEEVT 1545

  Fly  2998 IGSYSYEPNAFKFGDDIYSYSQLKLPERHF------WQRNFHLSFDFRSFYPNGMLYLSPGSKEK 3056
            :.|.|..|.|.:   ..:.|..:....:.|      :.....|....::...|||::..  |.::
 Frog  1546 LCSLSSRPKAAR---QAHLYGGIANSRQEFNHIPNGFNEKSELFVRLKTHSSNGMIFYV--SDKE 1605

  Fly  3057 PKHYVALVLKDGQLVLVV-RGRRREELQLTAKLNDGEWHRVTISCHDRKVTMSVEIGRTDQKTSA 3120
            ..:::.|.|..|:|..:. .|:::..::...|.|||.||.|.......|..:.:...|..:.:.:
 Frog  1606 EDNFMTLFLSHGRLTFMFNNGQQKLRIKSQEKYNDGLWHSVLCIREKNKGRLIINGLRVLEDSIS 1670

  Fly  3121 QMKLPKKIGASQLLLVGGLPQSPVKVSSDLYVR-LEPFKGCLRRVSINNNTQDLARPGKHSNVGQ 3184
            ...:...:.|.  ..:||:  :|.:...::.:. ...|.|||..:.:|...  |..|.:..:|..
 Frog  1671 AAAVTWTVDAP--FYLGGV--APGRAIKNVQINSAYSFNGCLSNLQLNGRA--LTSPSQTFSVTP 1729

  Fly  3185 CFP-TVERGSYFPGD-AYAIYKKNFNVGKYLDLETEFRTSELSGILLSVSDPNGFPALSLELHNG 3247
            ||. .:|.|:|...: .|.|...:|:.|...:...|.|....||:|:.:.:.|| ..|:|.:..|
 Frog  1730 CFEGPMESGTYISSEGGYVILDDSFSTGLTFEFALEVRPRNNSGVLIHIQNVNG-EYLNLHMKQG 1793

  Fly  3248 NIIFSCDPGNGAPMRVESSLPTKYALCDNKWHNISALYDGEQIVLRIDQLPAVISVGNQGNAGKV 3312
            .:|.....||   ....:.:..|.::||.:||.|:.:.||..:.|.:|.  .:..|....|...:
 Frog  1794 QVIVKVKNGN---REFSTRVIPKQSICDGQWHRIAVIRDGNVVQLDVDS--EINHVVGPINTKVL 1853

  Fly  3313 QTRSPLYIGGLPESAPSGSLISRENFKGCIRHVSIRNER 3351
            ..:.|:::||:|.|....:|.:|.:|.||||:..| :||
 Frog  1854 DLKEPVFVGGVPGSLLGPTLTTRNSFTGCIRNFVI-DER 1891

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
wb	NP_723870.1	Laminin_N	153..398	CDD:459653
		EGF_Lam	401..450	CDD:238012
		EGF_Lam	505..552	CDD:238012
		Laminin_EGF	550..597	CDD:395007
		Laminin_B	660..813	CDD:459652	13/53 (25%)
		EGF_Lam	851..900	CDD:238012	23/48 (48%)
		Laminin_EGF	<943..971	CDD:395007	12/27 (44%)
		Laminin_EGF	974..1018	CDD:395007	23/51 (45%)
		EGF_Lam	1017..1065	CDD:238012	5/47 (11%)
		Laminin_EGF	1065..>1105	CDD:395007	14/39 (36%)
		EGF_Lam	1150..1197	CDD:238012	18/46 (39%)
		Laminin_EGF	1199..1247	CDD:395007	2/47 (4%)
		EGF_Lam	1247..1292	CDD:238012	1/44 (2%)
		Laminin_EGF	1294..1342	CDD:395007	6/47 (13%)
		Laminin_EGF	1340..1392	CDD:395007	0/51 (0%)
		Laminin_B	1486..1626	CDD:459652	16/139 (12%)
		EGF_Lam	1669..1716	CDD:238012	4/49 (8%)
		Laminin_EGF	1718..1767	CDD:395007	6/48 (13%)
		EGF_Lam	1772..1819	CDD:238012	4/46 (9%)
		YhaN	<1907..2141	CDD:443752	52/269 (19%)
		SMC_prok_B	<2123..>2390	CDD:274008	58/291 (20%)
		Laminin_II	2279..2400	CDD:368703	28/141 (20%)
		LamG	2399..>2492	CDD:473984	21/108 (19%)
		LamG	2586..2753	CDD:238058	50/178 (28%)
		Laminin_G_2	2804..2944	CDD:460494	36/141 (26%)
		LamG	3018..3167	CDD:238058	28/156 (18%)
		LamG	3192..3347	CDD:238058	43/155 (28%)
lama4	XP_002936983.2	EGF_Lam	81..120	CDD:238012	19/38 (50%)
		EGF_Lam	131..186	CDD:238012	22/70 (31%)
		EGF_Lam	188..240	CDD:238012	23/82 (28%)
		EGF_Lam	242..286	CDD:214543	19/95 (20%)
		EGF_Lam	289..333	CDD:214543	20/140 (14%)
		Laminin_I	393..649	CDD:310534	86/613 (14%)
		SCP-1	<519..>837	CDD:114219	92/449 (20%)
		Laminin_II	829..958	CDD:368703	25/139 (18%)
		LamG	975..1106	CDD:214598	32/156 (21%)
		LamG	1143..1299	CDD:238058	50/179 (28%)
		LamG	1329..1466	CDD:238058	41/163 (25%)
		LamG	1582..1714	CDD:238058	27/137 (20%)
		LamG	1743..1889	CDD:238058	42/152 (28%)