DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment axo and APP

DIOPT Version :9

Sequence 1:	NP_001246631.1	Gene:	axo / 43923	FlyBaseID:	FBgn0262870	Length:	2179	Species:	Drosophila melanogaster
Sequence 2:	NP_000475.1	Gene:	APP / 351	HGNCID:	620	Length:	770	Species:	Homo sapiens

Alignment Length:	551	Identity:	118/551 - (21%)
Similarity:	191/551 - (34%)	Gaps:	151/551 - (27%)

- Green bases have known domain annotations that are detailed below.

  Fly   117 EKCAGPGDPGPCKQYIYKWRYEPTTNECTNFIWGGCEGNPQNRFGTEAECLFHCIGGPHTLPPFL 181
            |.|:...:.|||:..|.:|.::.|..:|..|.:|||.|| :|.|.||..|:..|   ...:...|
Human   289 EVCSEQAETGPCRAMISRWYFDVTEGKCAPFFYGGCGGN-RNNFDTEEYCMAVC---GSAMSQSL 349

  Fly   182 QSTTREPSTTESSMLLGLPYTQSPAQSPDGMGGAEGGDGTTPVPIEQRGPELTFAETGQGKTFIF 246
            ..||:||...:.   :.||.|  .|.:||.:...          :|..|.|...|...:.|..:.
Human   350 LKTTQEPLARDP---VKLPTT--AASTPDAVDKY----------LETPGDENEHAHFQKAKERLE 399

  Fly   247 AKNNTFIQM----------------DGD---IIQTFQLRL--CREISFQFRTRL--PHGLLVYHN 288
            ||:...:..                ..|   :||.||.::  ..:.:...|.:|  .|...|...
Human   400 AKHRERMSQVMREWEEAERQAKNLPKADKKAVIQHFQEKVESLEQEAANERQQLVETHMARVEAM 464

  Fly   289 VKNPDRINLDPY--ALYVIVEKGQLKVVHVFGKHSTSVTVGESLNRDEWHSVM----VR-IDVHG 346
            :.:..|:.|:.|  ||..:..:.:    |||......|...:   :|..|::.    || :|...
Human   465 LNDRRRLALENYITALQAVPPRPR----HVFNMLKKYVRAEQ---KDRQHTLKHFEHVRMVDPKK 522

  Fly   347 ARLIARVDNSQEEVYLKGLNHEYNYGVST--NLPSV---------------------VLVGGLSS 388
            |..|    .||...:|:.:....|..:|.  |:|:|                     ||...:|.
Human   523 AAQI----RSQVMTHLRVIYERMNQSLSLLYNVPAVAEEIQDEVDELLQKEQNYSDDVLANMISE 583

  Fly   389 EEKLHGVKYITESFVGCIRNVVLSSGKAASDLLPIAPLVATKHENVNEGCSDMCESRHNLCFVGS 453
            ....:|...:..|         |:..|...:|||:.          .|...|..:..|:
Human   584 PRISYGNDALMPS---------LTETKTTVELLPVN----------GEFSLDDLQPWHS------ 623

  Fly   454 RCINHYGGISCDCFGTHYEGEHCDIYTAT---IITLRGASYVSYRIYDWKDRVHSSTRRISLMFR 515
                 :|..|... .|..|.|..|...|.   :.|..|:...:.:           |..||.:..
Human   624 -----FGADSVPA-NTENEVEPVDARPAADRGLTTRPGSGLTNIK-----------TEEISEVKM 671

  Fly   516 TNFDDSALFYASGESLKHQ---YIAASI-KNQSVHVEMDFGDNVMSTVLTDDLTRGYWHNLTILH 576
                |:...:.||..:.||   :.|..: .|:...:.:..|..|::||:...|........|.:|
Human   672 ----DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIATVIVITLVMLKKKQYTSIH 732

  Fly   577 ------------EQRTVSIILDQQQKVLELP 595
                        |:|.:|   ..||...|.|
Human   733 HGVVEVDAAVTPEERHLS---KMQQNGYENP 760

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
axo	NP_001246631.1	KU	119..171	CDD:238057	19/51 (37%)
		Laminin_G_2	275..411	CDD:280389	33/167 (20%)
		EGF_CA	433..476	CDD:238011	8/42 (19%)
		Laminin_G_2	514..640	CDD:280389	21/98 (21%)
		Laminin_G_2	698..817	CDD:280389
		EGF_CA	842..878	CDD:238011
		Laminin_G_2	1112..1240	CDD:280389
		EGF_CA	1256..1297	CDD:238011
		Laminin_G_2	1351..1503	CDD:280389
		LamG	1612..1733	CDD:304605
APP	NP_000475.1	A4_EXTRA	24..188	CDD:128326
		GFLD subdomain. /evidence=ECO:0000255\|PROSITE-ProRule:PRU01217	28..123
		Heparin-binding. /evidence=ECO:0000269\|PubMed:8158260	96..110
		CuBD subdomain. /evidence=ECO:0000255\|PROSITE-ProRule:PRU01217	131..189
		Zinc-binding	181..188
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	194..284
		Kunitz_BPTI	294..341	CDD:333766	18/47 (38%)
		OX-2. /evidence=ECO:0000269\|PubMed:2649245	344..365		5/23 (22%)
		APP_E2	366..548	CDD:372388	41/204 (20%)
		Heparin-binding	391..423		3/31 (10%)
		Heparin-binding	491..522		7/33 (21%)
		Collagen-binding. /evidence=ECO:0000269\|PubMed:8576160	523..540		5/20 (25%)
		Beta-APP	677..713	CDD:367525	8/35 (23%)
		Interaction with PSEN1. /evidence=ECO:0000269\|PubMed:30630874	695..722		6/26 (23%)
		APP_amyloid	716..766	CDD:371108	10/48 (21%)
		Basolateral sorting signal	724..734		2/9 (22%)
		Interaction with G(o)-alpha	732..751		4/21 (19%)
		Required for the interaction with KIF5B and for anterograde transport in axons. /evidence=ECO:0000269\|PubMed:17062754	756..770		2/5 (40%)
		YENPXY motif, contains endocytosis signal. /evidence=ECO:0000269\|PubMed:10383380	757..762		2/4 (50%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG4295
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	2	1.810