DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Rok and Rock1

DIOPT Version :9

Sequence 1:	NP_536796.2	Gene:	Rok / 43916	FlyBaseID:	FBgn0026181	Length:	1390	Species:	Drosophila melanogaster
Sequence 2:	NP_033097.1	Gene:	Rock1 / 19877	MGIID:	107927	Length:	1354	Species:	Mus musculus

Alignment Length:	1405	Identity:	620/1405 - (44%)
Similarity:	860/1405 - (61%)	Gaps:	121/1405 - (8%)

- Green bases have known domain annotations that are detailed below.

  Fly    21 RANTLEREMRDPTSICNVDCLLDTVSALVSDCDHESLRRLKNIEQYAAKYKPLAMQINQLRMNVE 85
            |...::..:|||.|..|.|||||.:.|||.|.|..:||:.|||:.:.::||....:|..|||..|
Mouse    10 RFEKIDNLLRDPKSEVNSDCLLDGLDALVYDLDFPALRKNKNIDNFLSRYKDTINKIRDLRMKAE 74

  Fly    86 DFHFIKLIGAGAFGEVQLVRHKSSSQVYAMKRLSKFEMMKRPDSAFFWEERHIMAHANSEWIVQL 150
            |:..:|:||.|||||||||||||:.:|||||.||||||:||.|||||||||.|||.|||.|:|||
Mouse    75 DYEVVKVIGRGAFGEVQLVRHKSTRKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQL 139

  Fly   151 HFAFQDAKYLYMVMDFMPGGDIVSLMGDYDIPEKWAIFYTMEVVLALDTIHNMGFVHRDVKPDNM 215
            .:||||.:||||||::|||||:|:||.:||:|||||.|||.|||||||.||:|||:|||||||||
Mouse   140 FYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWARFYTAEVVLALDAIHSMGFIHRDVKPDNM 204

  Fly   216 LLDSYGHLKLADFGTCMRMGANGQVVSSNAVGTPDYISPEVLQSQGVDNEYGRECDWWSVGIFLY 280
            |||..||||||||||||:|...|.|....|||||||||||||:|||.|..|||||||||||:|||
Mouse   205 LLDKSGHLKLADFGTCMKMNKEGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLY 269

  Fly   281 EMLFGETPFYADSLVGTYGKIMDHKNSLSFPPEVEISEQAKALIRAFLTDRTQRLGRYGIEDIKA 345
            |||.|:||||||||||||.|||:|||||:||.:.:||::||.||.||||||..||||.|:|:||.
Mouse   270 EMLVGDTPFYADSLVGTYSKIMNHKNSLTFPDDNDISKEAKNLICAFLTDREVRLGRNGVEEIKR 334

  Fly   346 HPFFRNDTWSFDNIRESVPPVVPELSSDDDTRNFEDIERDEKPEEVFPVPKGFDGNHLPFIGFTY 410
            |.||:||.|:::.:|::|.||||:||||.||.||:|:|.|:..||.||:||.|.||.|||:||||
Mouse   335 HLFFKNDQWAWETLRDTVAPVVPDLSSDIDTSNFDDLEEDKGDEETFPIPKAFVGNQLPFVGFTY 399

  Fly   411 TGDYQLLSSDTVDAESKEANVANSGAASNNHGHGHNHRHRPSNSNELKRLEALLERERGRSEALE 475
            ..:.:.|.|.........:||..|              .:.|....:.:||..|..|....:.:|
Mouse   400 YSNRRYLPSANASENRSSSNVDKS--------------LQESLQKTIYKLEEQLHNEMQLKDEME 450

  Fly   476 QQDAGLRQQIELITK-------REAELQRIASEYEKDLALRQHNYKVAMQKVEQEIELRKKTEAL 533
            |:......:::.|.|       :...|:...|:.||:..|.||......:|||||.|.|
Mouse   451 QKCRTSNLKLDKIMKELDEEGNQRRNLESAVSQIEKEKMLLQHRINEYQRKVEQENEKR------ 509

  Fly   534 LVETQRNLENEQKT---RARDLN--------INDKVVSLEKQLLEMEQSYKTETENTQKLKKHNA 587
                 ||:|||..|   :..||.        .|:|:..|:|||.|.....:||::...:|:|.:.
Mouse   510 -----RNIENEVSTLKDQLEDLRKASQTSQLANEKLTQLQKQLEEANDLLRTESDTAVRLRKSHT 569

  Fly   588 ELDFTVKSQEEKVRDMVDMIDTLQKHKEELGQENAELQALVVQEKNLRSQLKEMHKEAENKMQTL 652
            |:..::...|...|::.:....|:..|.:..::..:|||::..|:..|....||..:.:.::.:|
Mouse   570 EMSKSISQLESLNRELQERNRILENSKSQADKDYYQLQAVLEAERRDRGHDSEMIGDLQARITSL 634

  Fly   653 INDIERTMCREQKAQEDNRALLEKISDLEK----AHAGLDFELKAAQGRYQQEVKAHQETEKSRL 713
            ..:::......::.:.:.:...:.::..||    ....|:::||:.|.|.:|||..|:.| |:||
Mouse   635 QEEVKHLKHNLERVEGERKEAQDMLNHSEKEKNNLEIDLNYKLKSIQQRLEQEVNEHKVT-KARL 698

  Fly   714 VSREEANLQEVKA-----LQSKLNEEKSARIKADQHSQEKERQLSMLSVDYRQIQLRLQKL-EGE 772
            ..:.: :::|.|:     ::.||.||:.||.||:....|.|:|.|||.||.:|.|.:|:.| |.:
Mouse   699 TDKHQ-SIEEAKSVAMCEMEKKLKEEREAREKAENRVVETEKQCSMLDVDLKQSQQKLEHLTENK 762

  Fly   773 CRQESEKVAALQSQLDQEHSKRNALLSELSLHSSEVAHLRSRENQLQKELSTQREAKRRFEEDLT 837
            .|.|.| |..|..||:||.:||..|.:||...:.|..:|:..|.|:::|::|..||||..|.:|.
Mouse   763 ERMEDE-VKNLALQLEQESNKRLLLQNELKTQAFEADNLKGLEKQMKQEINTLLEAKRLLEFELA 826

  Fly   838 QLKSTHHEALANNRELQAQLEAEQCFSRLYKTQANENREE----SAERLSKIEDLEEERVSLKHQ 898
            ||...:.......||||.||||||.||.|||||..|.:||    :.|.|.||::|:.|:.:|..|
Mouse   827 QLTKQYRGNEGQMRELQDQLEAEQYFSTLYKTQVKELKEEIEEKNRENLRKIQELQSEKETLSTQ 891

  Fly   899 VQVAVARADSEALARSIAEETVADLEKEKTIKELELKDFVMKHRNEINAKEAALATLKEAENELH 963
            :.:|..:|:||.|||.|.||...:|.:|.       |....::|.||..|:..::.|:|..:.|.
Mouse   892 LDLAETKAESEQLARGILEEQYFELTQES-------KKAASRNRQEITDKDHTVSRLEETNSVLT 949

  Fly   964 KKLGQKAAEYEDLVQQHKKQQEELALMRSSKDEEITKL---LDK-CKNEVLLKQVAVNKLAEVMN 1024
            |.:.....|.|:|.::.:..:||..|   .|:|||..|   .:| ...|..||..|||||||:||
Mouse   950 KDIEMLRKENEELNERMRTAEEEYKL---KKEEEINNLKAAFEKNISTERTLKTQAVNKLAEIMN 1011

  Fly  1025 RRDSDLPKQKNKARSTAELRKKEKEMRRLQQELSQERDKFNQLLLKHQ----DLQQLCAEEQQLK 1085
            |:|..:.::|   .:|.:|||||||.|:||.||:|||:||||:::|||    |:|....||...:
Mouse  1012 RKDFKIDRKK---ANTQDLRKKEKENRKLQLELNQEREKFNQMVVKHQKELNDMQAQLVEECTHR 1073

  Fly  1086 QKMVMEIDCKATEIENLQSKL------NETASLSSAD----NDPEDSQHSSLLSLTQDSVFEGWL 1140
            .::.|::..|.::||.|::||      ...||..|||    |.||             |..||||
Mouse  1074 NELQMQLASKESDIEQLRAKLLDLSDSTSVASFPSADETDGNLPE-------------SRIEGWL 1125

  Fly  1141 SVPNKQNRRRGHGWKRQYVIVSSRKIIFYNSDIDKHNTTDAVLILDLSKVYHVRSVTQGDVIRAD 1205
            ||||:.|.:| :|||:|||:|||:||:|||.:.||..::.: ::||:.|::|||.||||||.||:
Mouse  1126 SVPNRGNIKR-YGWKKQYVVVSSKKILFYNDEQDKEQSSPS-MVLDIDKLFHVRPVTQGDVYRAE 1188

  Fly  1206 AKEIPRIFQLLYAGEGASHRPDEQSQLDVSVLHGNCNEERPGTIVHKGHEFVHITYHMPTACEVC 1270
            .:|||:|||:|||.||...:..|...:.        ..|:.....||||||:...||.|..||.|
Mouse  1189 TEEIPKIFQILYANEGECRKDIEVEPVQ--------QGEKTNFQNHKGHEFIPTLYHFPANCEAC 1245

  Fly  1271 PKPLWHMFKPPAAYECKRCRNKIHKEHVD-KHDPLAPCKLNHDPRSARDMLLLAATPEDQSLWVA 1334
            .|||||:||||.|.||:||..|.|::|:| |.|.::|||:::|..|||||||||.:.::|..||.
Mouse  1246 AKPLWHVFKPPPALECRRCHVKCHRDHLDKKEDLISPCKVSYDVTSARDMLLLACSQDEQKKWVT 1310

  Fly  1335 RLLKRIQK---SGYKAASYNNNSTDGSKISPSQSTRSSYK 1371
            .|:|:|.|   ||:..||....||   :.:.:||.|...|
Mouse  1311 HLVKKIPKNPPSGFVRASPRTLST---RSTANQSFRKVVK 1347

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Rok	NP_536796.2	STKc_ROCK	61..411	CDD:270747	241/349 (69%)
		S_TKc	87..349	CDD:214567	193/261 (74%)
		SbcC	484..1088	CDD:223496	217/643 (34%)
		Rho_Binding	963..1027	CDD:286056	26/67 (39%)
		PH_ROCK	1134..1250	CDD:269948	54/115 (47%)
		C1	1254..1307	CDD:197519	29/53 (55%)
Rock1	NP_033097.1	STKc_ROCK1	2..406	CDD:270772	260/395 (66%)
		S_TKc	76..338	CDD:214567	193/261 (74%)
		Interaction with FHOD1. /evidence=ECO:0000250	368..727		101/385 (26%)
		Smc	<422..>1097	CDD:224117	232/715 (32%)
		HR1_ROCK1	489..554	CDD:212029	25/75 (33%)
		SHROOM3 binding. /evidence=ECO:0000269\|PubMed:22493320	707..946		98/246 (40%)
		Rho_Binding	949..1014	CDD:286056	26/67 (39%)
		RHOA binding. /evidence=ECO:0000250	998..1010		9/11 (82%)
		Auto-inhibitory. /evidence=ECO:0000250	1115..1354		119/259 (46%)
		PH_ROCK	1119..1225	CDD:269948	54/115 (47%)
		C1	1229..1283	CDD:237996	29/53 (55%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1333..1354		6/18 (33%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167833268
Domainoid	1	1.000	414	1.000	Domainoid score	I670
eggNOG	1	0.900	-	-		E2759_KOG0612
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	1070	1.000	Inparanoid score	I250
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG46537
OrthoDB	1	1.010	-	-		D36370at33208
OrthoFinder	1	1.000	-	-		FOG0001986
OrthoInspector	1	1.000	-	-		otm44051
orthoMCL	1	0.900	-	-		OOG6_104177
Panther	1	1.100	-	-	LDO	PTHR22988
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2768
SonicParanoid	1	1.000	-	-		X1475
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	15	14.800