Protein Alignment AMPKalpha and KIN4

DIOPT Version :10

• Sequence 1: NP_477313.1
• Gene: AMPKalpha / 43904
• FlyBaseID: FBgn0023169
• Length: 582
• Species: Drosophila melanogaster
• Sequence 2: NP_014876.1
• Gene: KIN4 / 854408
• SGDID: S000005759
• Length: 800
• Species: Saccharomyces cerevisiae
• Alignment Length: 690
• Identity: 185/690 - (26%)
• Similarity: 279/690 - (40%)
• Gaps: 197/690 - (28%)

  Fly    26 GHYLLGATLGTGTFGKVKIG------EHQITRVKVAVKILNRQKI-KSLDVVGKIRREIQNLKLF 83
            |.|::|:|||.|.|||||:|      .:::.: :||:|::.|..| |..|...||.|||..||..
Yeast    44 GPYIIGSTLGEGEFGKVKLGWTKASSSNEVPK-QVAIKLIRRDTIKKDADKEIKIYREINALKHL 107

  Fly    84 RHPHIIKLYQVISTPSDIFMIMEYVSGGELFDYIVKHGKLQEHQARRFFQQIISGVDYCHRHMIV 148
            .||:||.|.:|:.....|.:::|:|||||.:.||.:..:|:|..|.|.|.|:||||:|.|...:|
Yeast   108 THPNIIYLEEVLQNSKYIGIVLEFVSGGEFYKYIQRKRRLKESSACRLFAQLISGVNYMHYKGLV 172

  Fly   149 HRDLKPENLLLDHNMHVKIADFGLSNMML-DGEFLRTSCGSPNYAAPE-VISGKLYAGPEVDIWS 211
            |||||.||||||.:.::.|.|||..|... |.|.::||||||.||||| |:|.|.|...:.|:||
Yeast   173 HRDLKLENLLLDKHENLVITDFGFVNEFFEDNELMKTSCGSPCYAAPELVVSTKAYEARKADVWS 237

  Fly   212 CGVILYALLCGTLPFDDEH-------VPTLFRKIKSGIFPIPEYLNKQVVNLVCQMLQVDPLKRA 269
            |||||||:|.|.||:||:|       :..|::.|.......|||:.....:|:.::|..:|.:|.
Yeast   238 CGVILYAMLAGYLPWDDDHENPTGDDIARLYKYITQTPLKFPEYITPIPRDLLRRILVPNPRRRI 302

  Fly   270 NIEEIKKHEW------------------FQKDLP-----------------AYLFPSSIEQDSNV 299
            |::.||:|.|                  .||:.|                 |..:..|.:::|.:
Yeast   303 NLQTIKRHVWLKPHEAFLSIQPNYWDEHLQKERPKPPNKGDVGRHSTYSSSASSYSKSRDRNSLI 367

  Fly   300 ID--------------------TYAVAEVC----TKFGVKETEVHNSLLS------------GDP 328
            |:                    |::.....    ||..:||:.::....|            |..
Yeast   368 IESTLEQHRMSPQLATSRPASPTFSTGSKVVLNDTKNDMKESNINGERTSASCRYTRDSKGNGQT 432

  Fly   329 HDQLAIAYHLIIDNK-----------------RFADDAANQINE-----------------INNF 359
            ..:...|.|....||                 |..:..::::.|                 |.|:
Yeast   433 QIEQVSARHSSRGNKHTSVAGLVTIPGSPTTARTRNAPSSKLTEHVKDSSQTSFTQEEFHRIGNY 497

  Fly   360 FVAGSPPPPPP--PPVPQSSMDHQAPLATVTVG--GGTSASSGTATPVP---------------- 404
            .|..|.|.|..  |.:.:::.|:.  ||.:.|.  |.....:....|||
Yeast   498 HVPRSRPRPTSYYPGLSRNTADNS--LADIPVNKLGSNGRLTDAKDPVPLNAIHDTNKATISNNS 560

  Fly   405 --PVAGGTPSSTIPIRPHPERIAPMRDRQLAMSVQTSGGGAFPE-----------KTARGGTPIK 456
              .::.|..:.|.|:           |...|:.....|.....|           |.|..|.|
Yeast   561 IMLLSEGPAAKTSPV-----------DYHYAIGDLNHGDKPITEVIDKINKDLTHKAAENGFP-- 612

  Fly   457 RAKWHLGIRSQSKPND-----IMLEVYRAMKALSYEWKIINPYHVRVRRQNVKTGKFSKMSLQLY 516
                    |....|..     :..|...:......|.::.|..|    ..|.......|.|.::|
Yeast   613 --------RESIDPESTSTILVTKEPTNSTDEDHVESQLENVGH----SSNKSDASSDKDSKKIY 665

  Fly   517 QVDAKSYLLDFKSLTNDEVEQGDDVIMESLT----PPPLS 552
            :....|::..:.||      .|....:||.|    .||:|
Yeast   666 EKKRFSFMSLYSSL------NGSRSTVESRTSKGNAPPVS 699

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
AMPKalpha	NP_477313.1	STKc_AMPK_alpha	25..280	CDD:270981	119/287 (41%)
		UBA_AID_AMPKalpha	297..360	CDD:270521	17/132 (13%)
		AMPKA_C	457..580	CDD:213378	20/105 (19%)
KIN4	NP_014876.1	STKc_Kin4	44..313	CDD:270978	118/269 (44%)