Protein Alignment AMPKalpha and HSL1

DIOPT Version :10

• Sequence 1: NP_477313.1
• Gene: AMPKalpha / 43904
• FlyBaseID: FBgn0023169
• Length: 582
• Species: Drosophila melanogaster
• Sequence 2: NP_012821.2
• Gene: HSL1 / 853760
• SGDID: S000001584
• Length: 1518
• Species: Saccharomyces cerevisiae
• Alignment Length: 694
• Identity: 176/694 - (25%)
• Similarity: 305/694 - (43%)
• Gaps: 154/694 - (22%)

  Fly    11 AVAAGSANGQPLVKIGHYLLGATLGTGTFGKVKIGEHQITRVKVAVKILNRQK------------ 63
            :||..|:..:....:|.:.||.|||.|:.|:|::.::..|....|:||:.::|
Yeast    64 SVATKSSKRKSRDTVGPWKLGKTLGKGSSGRVRLAKNMETGQLAAIKIVPKKKAFVHCSNNGTVP 128

  Fly    64 -----------IKSLDVVGK------------IRREIQNLKLFRHPHIIKLYQVISTPSDIFMIM 105
                       :.|..:..:            |.|||..:||..|.:::.|::|....|::::::
Yeast   129 NSYSSSMVTSNVSSPSIASREHSNHSQTNPYGIEREIVIMKLISHTNVMALFEVWENKSELYLVL 193

  Fly   106 EYVSGGELFDYIVKHGKLQEHQARRFFQQIISGVDYCHRHMIVHRDLKPENLLLD-HNMHVKIAD 169
            |||.|||||||:|..|||.|.:|..:|:||:.||.|||...|.|||||||||||| .|..:||||
Yeast   194 EYVDGGELFDYLVSKGKLPEREAIHYFKQIVEGVSYCHSFNICHRDLKPENLLLDKKNRRIKIAD 258

  Fly   170 FGLSNMMLDGEFLRTSCGSPNYAAPEVISGKLYAGPEVDIWSCGVILYALLCGTLPFDDEHVPTL 234
            ||::.:.|..:.|:||||||:||:||::.|:.|.|...|:||||::|:|||.|.|||:|:::..|
Yeast   259 FGMAALELPNKLLKTSCGSPHYASPEIVMGRPYHGGPSDVWSCGIVLFALLTGHLPFNDDNIKKL 323

  Fly   235 FRKIKSGIFPIPEYLNKQVVNLVCQMLQVDPLKRANIEEIKKHEWFQK--DLPAYLFPSSIEQDS 297
            ..|::||.:.:|..|:.:..:|:.::|.:||.||...:||.||...:|  |||.......:.:|:
Yeast   324 LLKVQSGKYQMPSNLSSEARDLISKILVIDPEKRITTQEILKHPLIKKYDDLPVNKVLRKMRKDN 388

  Fly   298 ------------------NVIDTYAVAEVCTKF---------GVKETEVHNSLLSGDPHDQLAIA 335
                              :::..::..|:....         ||....:...||. .|..:..:.
Yeast   389 MARGKSNSDLHLLNNVSPSIVTLHSKGEIDESILRSLQILWHGVSRELITAKLLQ-KPMSEEKLF 452

  Fly   336 YHLII--------------DNKR-------------FADDAAN------QINEI----------- 356
            |.|::              :||:             :|...||      :.|::
Yeast   453 YSLLLQYKQRHSISLSSSSENKKSATESSVNEPRIEYASKTANNTGLRSENNDVKTLHSLEIHSE 517

  Fly   357 ------NNFFVAGSPPPPPPPPVPQSSMDHQAPLATVTVGGGTSASSGTAT--PVPPVAGGTPS- 412
                  .|..:.|.......|.:.|.|   |..:.|::......|.:...|  |..|:...:.|
Yeast   518 DTSTVNQNNAITGVNTEINAPVLAQKS---QFSINTLSQPESDKAEAEAVTLPPAIPIFNASSSR 579

  Fly   413 ----STIPIRPHPERIAPMRDRQLAMSVQTSGGGAFPEKTARGGTPIKRAKWHLGIRSQSKPNDI 473
                |...|.....|...:.:.:|::|..||.......:......|...:::.|..|:
Yeast   580 IFRNSYTSISSRSRRSLRLSNSRLSLSASTSRETVHDNEMPLPQLPKSPSRYSLSRRA------- 637

  Fly   474 MLEVYRAMKALSYEWKIINPYHVRVRRQNVKTGKFSKMSLQLYQVDAKSYLLDFKSLTNDEVEQG 538
             :....:.|::          |..:.|:|:.....::.:||  ...:|..|...:|::...:...
Yeast   638 -IHASPSTKSI----------HKSLSRKNIAATVAARRTLQ--NSASKRSLYSLQSISKRSLNLN 689

  Fly   539 DDVIMESLTPPPLSVS-GVMPLQPTGHHTME----FFEMCAALI 577
            |.::.:...|.....| .|...:|   |::|    |..:|..::
Yeast   690 DLLVFDDPLPSKKPASENVNKSEP---HSLESDSDFEILCDQIL 730

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
AMPKalpha	NP_477313.1	STKc_AMPK_alpha	25..280	CDD:270981	114/290 (39%)
		UBA_AID_AMPKalpha	297..360	CDD:270521	15/139 (11%)
		AMPKA_C	457..580	CDD:213378	20/126 (16%)
HSL1	NP_012821.2	STKc_BRSK1_2	79..369	CDD:270983	114/289 (39%)
		AMPKA_C_like	1347..1517	CDD:473060