DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment AMPKalpha and mark2a

DIOPT Version :9

Sequence 1:	NP_477313.1	Gene:	AMPKalpha / 43904	FlyBaseID:	FBgn0023169	Length:	582	Species:	Drosophila melanogaster
Sequence 2:	XP_009293774.1	Gene:	mark2a / 562231	ZFINID:	ZDB-GENE-030131-4145	Length:	782	Species:	Danio rerio

Alignment Length:	652	Identity:	201/652 - (30%)
Similarity:	315/652 - (48%)	Gaps:	126/652 - (19%)

- Green bases have known domain annotations that are detailed below.

  Fly    16 SANGQPLVKIGHYLLGATLGTGTFGKVKIGEHQITRVKVAVKILNRQKIKSLDVVGKIRREIQNL 80
            :|:.||  .||.|.|..|:|.|.|.|||:.:|.:|..:|||||:::.::.| ..:.|:.||::.:
Zfish    34 TADEQP--HIGCYRLLKTIGKGNFAKVKLAKHILTGKEVAVKIIDKTQLNS-SSLQKLFREVRIM 95

  Fly    81 KLFRHPHIIKLYQVISTPSDIFMIMEYVSGGELFDYIVKHGKLQEHQARRFFQQIISGVDYCHRH 145
            ||..||:|:||::||.|...::::|||.||||:|||:|.||:::|.:||..|:||:|.|.|||:.
Zfish    96 KLLNHPNIVKLFEVIETEKTLYLVMEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQK 160

  Fly   146 MIVHRDLKPENLLLDHNMHVKIADFGLSNMMLDGEFLRTSCGSPNYAAPEVISGKLYAGPEVDIW 210
            .|||||||.||||||.:|::||||||.||....|..|.|.||||.|||||:..||.|.|||||:|
Zfish   161 CIVHRDLKAENLLLDADMNIKIADFGFSNEFTLGNKLDTFCGSPPYAAPELFQGKKYDGPEVDVW 225

  Fly   211 SCGVILYALLCGTLPFDDEHVPTLFRKIKSGIFPIPEYLNKQVVNLVCQMLQVDPLKRANIE-EI 274
            |.|||||.|:.|:||||.:::..|..::..|.:.||.|::....||:.:.|.::|.||.::| :|
Zfish   226 SLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKKFLVLNPTKRGSLEQQI 290

  Fly   275 KKHEWF-----QKDLPAYLFPSSIEQDSNVIDTYAVA------EVCTKFGVKETEVHNSLLSGDP 328
            .|..|.     .::|..|:.|....:|......:..:      ::..:.|....|:.:||::...
Zfish   291 MKDRWMNVGHEDEELKPYIEPQPDYKDPKRTGQHPSSAGGWKRDIMIRMGYSLDEIQDSLINQKY 355

  Fly   329 HDQLAIAYHLIID--------------------------------------NKRFADDA--ANQI 353
            :|  .:|.:|::|                                      ::|..|.:  :::.
Zfish   356 ND--VMATYLLLDYRNSELDELSIKARPGTDITNNAQSPSHKGQRSTSNLKSRRSTDQSSVSSKR 418

  Fly   354 NEINNFFVAGSPPPPPPPPVPQSSMDHQ--------------------APL---ATVTVGG---- 391
            .:.::..:..|......||.|..|.||:                    :|:   ||:.|..
Zfish   419 PQGDSKHIGSSGSSSKVPPSPLISGDHKKTPTPSTNSILSSGTGRSRNSPITERATLGVQNGKDS 483

  Fly   392 ----GTSASSGTATPVPPVAGGT------PSSTIPIRP----HPERIAPMRDRQLAMSVQTSGGG 442
                |:.||:.:|..|...:..|      .:|..|..|    |....|..|...::.|.|.....
Zfish   484 LHTPGSRASTASAAAVLAASSRTRHHKSLSTSAHPSPPDLHAHRPSTANQRAPVVSPSAQNINIS 548

  Fly   443 AFPEKT--ARGGTPIKRAKWHLGIRSQSKPNDIMLEVYR---AMKALSYEWKIINPYHVRVRRQN 502
            :..::|  :||.|  .|:.:|.|  .|....|.....|.   |..:||:.       :.:|||..
Zfish   549 SMADRTNFSRGVT--SRSTFHAG--QQRATRDQQASAYNDPSASPSLSHG-------NSQVRRPG 602

  Fly   503 VKTGKFSKMSLQLYQVDAKSYLLDFKSLTNDEVE-QGDDVIMESLTPPPLSVSGVMPLQPTGHHT 566
              ||.|||.:.:..:.:     |.|:.......| :|.|    ..:.|.||.:..|.....||.:
Zfish   603 --TGIFSKFTSKFVRRN-----LSFRFPRRSPYEGEGRD----EASRPMLSTADKMEKGTQGHPS 656

  Fly   567 ME 568
            .|
Zfish   657 DE 658

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
AMPKalpha	NP_477313.1	STKc_AMPK_alpha	25..280	CDD:270981	125/255 (49%)
		UBA_AID_AMPKalpha	297..360	CDD:270521	10/108 (9%)
		AMPKA_C	457..580	CDD:213378	29/116 (25%)
mark2a	XP_009293774.1	STKc_MARK	44..296	CDD:270974	123/252 (49%)
		S_TKc	44..296	CDD:214567	123/252 (49%)
		UBA_MARK2	314..367	CDD:270589	8/54 (15%)
		MARK2_C	683..781	CDD:213386
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	1	0.910