DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment AMPKalpha and ssp2

DIOPT Version :9

Sequence 1:	NP_477313.1	Gene:	AMPKalpha / 43904	FlyBaseID:	FBgn0023169	Length:	582	Species:	Drosophila melanogaster
Sequence 2:	NP_588376.1	Gene:	ssp2 / 2539109	PomBaseID:	SPCC74.03c	Length:	576	Species:	Schizosaccharomyces pombe

Alignment Length:	584	Identity:	222/584 - (38%)
Similarity:	336/584 - (57%)	Gaps:	69/584 - (11%)

- Green bases have known domain annotations that are detailed below.

  Fly    25 IGHYLLGATLGTGTFGKVKIGEHQITRVKVAVKILNRQKIKSLDVVGKIRREIQNLKLFRHPHII 89
            ||.|::..|||.|:|||||:..|..|:.|||:|.::||.:|..|:..::.|||..|||.||||||
pombe    31 IGPYIIRETLGEGSFGKVKLATHYKTQQKVALKFISRQLLKKSDMHMRVEREISYLKLLRHPHII 95

  Fly    90 KLYQVISTPSDIFMIMEYVSGGELFDYIVKHGKLQEHQARRFFQQIISGVDYCHRHMIVHRDLKP 154
            |||.||:||:||.|::|| :|||||||||:..::.|.:.||||||||..::|||||.||||||||
pombe    96 KLYDVITTPTDIVMVIEY-AGGELFDYIVEKKRMTEDEGRRFFQQIICAIEYCHRHKIVHRDLKP 159

  Fly   155 ENLLLDHNMHVKIADFGLSNMMLDGEFLRTSCGSPNYAAPEVISGKLYAGPEVDIWSCGVILYAL 219
            ||||||.|::||||||||||:|.||.||:||||||||||||||:||||||||||:||||::||.:
pombe   160 ENLLLDDNLNVKIADFGLSNIMTDGNFLKTSCGSPNYAAPEVINGKLYAGPEVDVWSCGIVLYVM 224

  Fly   220 LCGTLPFDDEHVPTLFRKIKSGIFPIPEYLNKQVVNLVCQMLQVDPLKRANIEEIKKHEWFQKDL 284
            |.|.||||||.:|.||:|:.|.::.:|::|:....:|:.:|:..||::|..|:||::..||..:|
pombe   225 LVGRLPFDDEFIPNLFKKVNSCVYVMPDFLSPGAQSLIRRMIVADPMQRITIQEIRRDPWFNVNL 289

  Fly   285 PAYLFPSSIEQDSNVIDTYAVAEVCTKFGVKETEVHNSLLSGDPHDQLAIAYHLIIDNKRFADDA 349
            |.||.|.. |...:..|:..|:::....|..|..:..:|.| |.::::..||:|:.:|:...:.:
pombe   290 PDYLRPME-EVQGSYADSRIVSKLGEAMGFSEDYIVEALRS-DENNEVKEAYNLLHENQVIQEKS 352

  Fly   350 -ANQINEINNFFVAGSPPPPPPPPVPQSSMDHQAPLATVTVGGGTSASSGTATPVPPVAGGTPSS 413
             .::...:::|....      ||...:.:.:.|.......:.........|.:..|..|..|..|
pombe   353 HLSKSKRVDSFLSVS------PPAFSEYTSELQKKSKQELIDPTLEGPRWTVSDPPTYAKQTIDS 411

  Fly   414 TIPIRPHPERIAPMRDR---------QLAMSVQTSGGGAFPEKTARGGTPIKRAKWHLGIRSQSK 469
            .|.:      :.|..::         ..|.:|.||      :.|.:..   :|.|||.|:|.:..
pombe   412 NICV------LVPTAEKNKLEMRTLADAASAVDTS------QSTRKKS---RRNKWHFGVRCRGD 461

  Fly   470 PNDIMLEVYRAMKALSYEWKIINPYHVRVRRQ--NVKT----------GK--FSKMSLQLYQVDA 520
            ..:|:|.||||::....::.:..|.:.:.|..  .:|:          ||  ::.:.||||:|..
pombe   462 APEILLAVYRALQRAGAQFTVPKPVNGKYRSDMYTIKSRWEIPHCKREGKNTYAYIELQLYEVMP 526

  Fly   521 KSYLLDFKSLTNDEV----EQGDDVIMESLTPPPLSVSGVMPLQPTGHHTMEFFEMCAALIIQL 580
            ..::||.||....::    |:..|..|:.|                 ..:..|.::||.|:.:|
pombe   527 GCFMLDVKSNGYKDIYSHPERTADHGMDDL-----------------KSSFPFLDLCAMLVCKL 573

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
AMPKalpha	NP_477313.1	STKc_AMPK_alpha	25..280	CDD:270981	152/254 (60%)
		UBA_AID_AMPKalpha	297..360	CDD:270521	11/63 (17%)
		AMPKA_C	457..580	CDD:213378	34/140 (24%)
ssp2	NP_588376.1	STKc_AMPK_alpha	31..285	CDD:270981	152/254 (60%)
		S_TKc	34..285	CDD:214567	150/251 (60%)
		UBA_SNF1_fungi	305..351	CDD:270519	11/46 (24%)
		AdenylateSensor	451..573	CDD:293187	33/138 (24%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Domainoid	1	1.000	329	1.000	Domainoid score	I211
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H4551
Inparanoid	1	1.050	381	1.000	Inparanoid score	I416
OMA	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000976
OrthoInspector	1	1.000	-	-		oto101706
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	LDO	PTHR24343
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1362
SonicParanoid	1	1.000	-	-		X997
TreeFam	1	0.960	-	-
	11	11.050