DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment sif and ARHGEF1

DIOPT Version :9

Sequence 1:	NP_001261446.1	Gene:	sif / 43892	FlyBaseID:	FBgn0085447	Length:	2734	Species:	Drosophila melanogaster
Sequence 2:	NP_945353.1	Gene:	ARHGEF1 / 9138	HGNCID:	681	Length:	927	Species:	Homo sapiens

Alignment Length:	922	Identity:	190/922 - (20%)
Similarity:	304/922 - (32%)	Gaps:	304/922 - (32%)

- Green bases have known domain annotations that are detailed below.

  Fly  1020 DQETRHQIQTQILQWEENLERLHCEQFRLR--CYMASLQSGEL---PNPKSLLTHVSRPTKNTLN 1079
            |::..::::|.  ..|:|.:....||.:.|  ..||.||...|   |.|.....|.     :.|.
Human    43 DEDFENELETN--SEEQNSQFQSLEQVKRRPAHLMALLQHVALQFEPGPLLCCLHA-----DMLG 100

  Fly  1080 KLG------VFTVSSFHAFICARSPSLLNNLLAGRGATKRRPPMLSRSNSGSSRRSMQMNSRDEP 1138
            .||      .| :..:|:|:
Human   101 SLGPKEAKKAF-LDFYHSFL--------------------------------------------- 119

  Fly  1139 EKT--FKVAMPDN-AYSTVYLRDAMSVEEFLASACARRNLNPMEHFVRVKKRRDMEDHNY----- 1195
            |||  .:|.:|.| |:.....|     .:.::....||.:..:....:|...|.:||...
Human   120 EKTAVLRVPVPPNVAFELDRTR-----ADLISEDVQRRFVQEVVQSQQVAVGRQLEDFRSKRLMG 179

  Fly  1196 FVPHRNDLIENYLHNHEFVEVCMKILYQVELQRTTLEQMWG---FSVEAELIENAER----QDEL 1253
            ..|...:|.:                         ||...|   .|.||.....|||    .:|:
Human   180 MTPWEQELAQ-------------------------LEAWVGRDRASYEARERHVAERLLMHLEEM 219

  Fly  1254 CCYVSRVEDKSVAMHNGIIKGDEIMVINGAIVSDLDMMYLESVLQEEQSLSMMMRSSRTEPPD-- 1316
            ...:|..|:||.|:.|.|                              .|.|.....||:..|
Human   220 QHTISTDEEKSAAVVNAI------------------------------GLYMRHLGVRTKSGDKK 254

  Fly  1317 ---------LVGIMRVTDDMIDSLVCPPPPTDPPVMSEEMITGLIVPAPGWN-GTSK--DLYSPE 1369
                     ::|..|               :|.|..:::.::. |:.|..|| |..:  |....:
Human   255 SGRNFFRKKVMGNRR---------------SDEPAKTKKGLSS-ILDAARWNRGEPQVPDFRHLK 303

  Fly  1370 AESSPATSFVDPAAMAAQLAVGGVG----------------GVVVGGLGVAKP------------ 1406
            ||       ||.....|....||||                ||.:..|.:..|
Human   304 AE-------VDAEKPGATDRKGGVGMPSRDRNIGAPGQDTPGVSLHPLSLDSPDREPGADAPLEL 361

  Fly  1407 --TSRTSSFEIENLL---KTAE-QETRKSSPTGSVTSSVSTTALTPS-----RQLTDAE------ 1454
              :|......:|:|.   .|.| .||....|........|...|.|.     |:|...:
Human   362 GDSSPQGPMSLESLAPPESTDEGAETESPEPGDEGEPGRSGLELEPEEPPGWRELVPPDTLHSLP 426

  Fly  1455 ----KLRKVVMELVDTERTYVKHLNNLLEHYLEPMKRETFLSNAEINALFGNIHEIVTFQRQFLQ 1515
                |.::|:.||:.||..:|:.|..|.:.:.:||....|....|:..:|.::.|::.....||.
Human   427 KSQVKRQEVISELLVTEAAHVRMLRVLHDLFFQPMAECLFFPLEELQNIFPSLDELIEVHSLFLD 491

  Fly  1516 NL----EESLDLEPDFNKFEHCGQFRNVLF--AIGSAFLYYVNHFKLYSSFCASHSKAQKVLHPN 1574
            .|    :||..|      .|..|......|  |.||.|.      |:.|.||:..|.|.:.|...
Human   492 RLMKRRQESGYL------IEEIGDVLLARFDGAEGSWFQ------KISSRFCSRQSFALEQLKAK 544

  Fly  1575 EGNHA-----LQEFLAARNPKQQHSSTLESYLIKPIQRILKYPLLLQQM-RNLTDTRADEHVHLC 1633
            :....     :||  |...|:.:... |:..:...:||:.|||||||.: :|..:....|.|.|
Human   545 QRKDPRFCAFVQE--AESRPRCRRLQ-LKDMIPTEMQRLTKYPLLLQSIGQNTEEPTEREKVEL- 605

  Fly  1634 EALKGMEKVAEHINEMQRIHEEYGAIFDHLFR---QHQKSCKQP-------IDLSPGDLLYYGGV 1688
             |.:...::..|:|:..|..|:...:.|:..|   .|.:....|       :|::...|::.|.:
Human   606 -AAECCREILHHVNQAVRDMEDLLRLKDYQRRLDLSHLRQSSDPMLSEFKNLDITKKKLVHEGPL 669

  Fly  1689 EWLNISDFLGKIKKGLELHAMCFVFKSAVVFLCKERL----RQKKKLMGVSSKNATNEVEIIRYQ 1749
            .|....|      |.:|:|          |.|..:.|    ||.::|: :.|.:.|.........
Human   670 TWRVTKD------KAVEVH----------VLLLDDLLLLLQRQDERLL-LKSHSRTLTPTPDGKT 717

  Fly  1750 VLIPV---TEVQVR--ASSAKDMDSHFLWELIHLRSQLQRRSEKVYVLSNSTADFRNAFLKTIRQ 1809
            :|.||   |....|  |:..|.....|.|:          :..::|.|...|...|    |....
Human   718 MLRPVLRLTSAMTREVATDHKAFYVLFTWD----------QEAQIYELVAQTVSER----KNWCA 768

  Fly  1810 IIRESVRNMSIP 1821
            :|.|:..::.:|
Human   769 LITETAGSLKVP 780

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937
		PH	873..974	CDD:278594
		TIAM1_RBD	1141..1219	CDD:176413	14/85 (16%)
		PDZ_signaling	1221..1308	CDD:238492	19/93 (20%)
		RhoGEF	1456..1647	CDD:238091	54/202 (27%)
		PH2_Tiam1_2	1650..1823	CDD:269957	39/191 (20%)
		PH	1693..1812	CDD:278594	26/127 (20%)
ARHGEF1	NP_945353.1	RGS_p115RhoGEF	67..259	CDD:188709	53/302 (18%)
		Trypan_PARP	<330..425	CDD:114603	17/94 (18%)
		RhoGEF	435..619	CDD:214619	54/200 (27%)
		PH_p115RhoGEF	653..777	CDD:275429	32/154 (21%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	1	0.910