DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sif and net1

DIOPT Version :9

Sequence 1:	NP_001261446.1	Gene:	sif / 43892	FlyBaseID:	FBgn0085447	Length:	2734	Species:	Drosophila melanogaster
Sequence 2:	NP_001072884.2	Gene:	net1 / 780346	XenbaseID:	XB-GENE-487220	Length:	585	Species:	Xenopus tropicalis

Alignment Length:	717	Identity:	122/717 - (17%)
Similarity:	241/717 - (33%)	Gaps:	231/717 - (32%)

- Green bases have known domain annotations that are detailed below.

  Fly  1329 DSLVCPPPPTDPP-----------VMSEEMITGL-------------------------IVPAPG 1357
            :.::.||....||           |..:..::.|                         :.|...
Frog     4 EEVMAPPAALTPPSENVKDKKRKGVKRKSSVSSLAGGQSTPEGGSSRRYSLRRGSSFTFLTPGSQ 68

  Fly  1358 WNGTSKDLYSPEAESS----------PATSFVDPAAMAAQLAVGGVGGVVVGGLGVAKPTSRTSS 1412
            |:.|.|.....:.:.:          |:|..|.|.:....||            .:..|....:.
Frog    69 WDFTLKRKRREKDDDALSLCSFDFKEPSTKRVRPLSRVTSLA------------NLISPVRNGAV 121

  Fly  1413 FEIENLLKTAEQETRKSSPTGSVTSSVSTTALTPSRQ-----------------LTDAE-KLRKV 1459
            ......:::........||.|:..:.....|.||.::                 |:..| |.::.
Frog   122 RRFGQTIQSFTMRAENKSPGGTQKACSKVAAPTPPKRRNSVLWSEMLDINMKESLSTKEIKRQEA 186

  Fly  1460 VMELVDTERTYVKHLNNLLEHYLEPMKRETFLSNAEINALFGNIHEIVTFQRQFLQNLEESLDLE 1524
            :.|:...|:..:..|....:.|.:||.:.:.:|..|:..:||::...:....:.|..|.|:...:
Frog   187 IFEMTKGEQDLIDDLKLARKAYHDPMLKLSIMSEEELTQIFGDLDSYIPLHEELLAKLGEATKAD 251

  Fly  1525 PDFNKFEHCGQFRNVLFAIGSAFLYYVNHFKLYSSFCASHSKAQKVLHPNEGNHALQEFLAARNP 1589
            ..      .||       ||...:.::.....|..:|::...|:.:|...:.:..:|:|| .|..
Frog   252 GT------VGQ-------IGPILINWLPRLNAYKEYCSNQLAAKALLDKKKLDRRVQDFL-QRCL 302

  Fly  1590 KQQHSSTLE--SYLIKPIQRILKYPLLLQQMRNLTDTRADEHVHLCEALKGMEKVAEHINEMQRI 1652
            :...|..|:  |:|..|..|::||||||:::...|.....:...|.|||..::.|...||
Frog   303 ESPFSRKLDLWSFLDIPRSRLVKYPLLLKEILRHTPKDHPDTKGLHEALCLIQGVLTDIN----- 362

  Fly  1653 HEEYGAIFDHLFRQHQKSCKQPIDLSPGDLLYYGGVEWLNISDFLGKIKKGLELHAMCFVFKSAV 1717
                       .::.:..|:           ||     ::..::|...:|.|.:       :|:.
Frog   363 -----------LKKGESECQ-----------YY-----IDKLEYLDDKQKDLRI-------ESSK 393

  Fly  1718 VFLCKERLRQKKKLMGVSSKNATNEVEIIRYQVLIPVTEVQVRASSAKDMDSHFLWELIHLRSQL 1782
            ..||...|:.|.          .:::.:..:|.::.:|...:|     :...||         |:
Frog   394 ALLCHGELKNKN----------GHKLFLFLFQDVLVLTRPVIR-----NEHQHF---------QV 434

  Fly  1783 QRRSEKV--YVLSN-------STADFRNAFLKTIRQIIRESVRNM-SIPMKNFG-GSSGSVSG-- 1834
            .|:...|  .||.:       ....||.||..:      :..:|: .:..|:.| |.|.::..
Frog   435 YRQPIPVQDLVLEDLQDGDVRMGGSFRGAFSNS------DKAKNIFRVRFKDAGAGQSHTLQAND 493

  Fly  1835 --HSSQGMGSMGYPGNSQTLERPKQQITIVHGSHTLGKPKKKSGSQRHSAGNIDYDNLSGSQEAD 1897
              |..|.:..:      :|...|.||          ..|.::.          :..:|:...|.:
Frog   494 VFHKQQWLNCI------KTAVSPYQQ----------ASPTEQK----------ELPDLNEECEEN 532

  Fly  1898 DLPPSVGVVHYASGHTHGQQMQPAGFRGRSKTVGDVTEI------------TCSSPEPHQQQQQQ 1950
            : ||:..    :..|.            ||.|:..:.|:            |..||:...:.||:
Frog   533 N-PPATN----SKDHR------------RSSTISGIMEMDLEENAFSSLLDTTESPKTRDKTQQR 580

  Fly  1951 QQ 1952
            ::
Frog   581 KE 582

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937
		PH	873..974	CDD:278594
		TIAM1_RBD	1141..1219	CDD:176413
		PDZ_signaling	1221..1308	CDD:238492
		RhoGEF	1456..1647	CDD:238091	43/192 (22%)
		PH2_Tiam1_2	1650..1823	CDD:269957	26/182 (14%)
		PH	1693..1812	CDD:278594	22/127 (17%)
net1	NP_001072884.2	RhoGEF	186..362	CDD:366202	43/189 (23%)
net1	NP_001072884.2	PH_Net1	376..510	CDD:270044	30/176 (17%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	0.910