DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sif and Akap13

DIOPT Version :9

Sequence 1:	NP_001261446.1	Gene:	sif / 43892	FlyBaseID:	FBgn0085447	Length:	2734	Species:	Drosophila melanogaster
Sequence 2:	XP_036009413.1	Gene:	Akap13 / 75547	MGIID:	2676556	Length:	2817	Species:	Mus musculus

Alignment Length:	2994	Identity:	535/2994 - (17%)
Similarity:	925/2994 - (30%)	Gaps:	1097/2994 - (36%)

- Green bases have known domain annotations that are detailed below.

  Fly    46 HVSASGAGTVKWQQVSEDLVPVNITCIQDSPECIFHITAYNSQVDKILDVRLVQPGTRIGQASEC 110
            |:|       |...:.:.|:..|:                 .|:|.::.:.:.      .|.|.|
Mouse   273 HIS-------KLMNIQQQLMKTNL-----------------KQMDNLMPLMVT------AQDSSC 307

  Fly   111 FVYWKDPMTNDTWGLNF---TSPIDAKQ--FRE-----CC--SPSFKFSRKASSSYSL------- 156
            .     |...:|.||..   ..|.|.:.  |.|     ||  ||    .|.|.||..|
Mouse   308 V-----PSVPETDGLFLPCVPEPSDHQHPPFEETKSTLCCQRSP----GRMAESSCDLSSMVEEE 363

  Fly   157 --------KLDPPGKGKVKAKRKPLSTPASPSRVRQE------PQC---------TC-----MSA 193
                    ..|...||:   :.:|.|...|.|...|:      |.|         :|     ::.
Mouse   364 NVICSHKKNKDVGRKGE---EAEPASAMDSGSASHQDSCLQSVPDCGVKGREGLPSCGNRNEVTG 425

  Fly   194 EQYARLRT--DPRVRGSSTLP---------------RNVGSHRITDVDGQQQVGSGKVVSAVSST 241
            ..|:.:.|  .|....||.|.               |.:.....|||...::.|..:........
Mouse   426 TNYSGVATCQQPLSSRSSVLQDAMVTEPDACQHSSGRELPDSSSTDVGAPEKAGELEHSLLTPDA 490

  Fly   242 SLYDNVASGGPGTNQ--------GADTLPRQMKGGQQDRQDVAN-----SGVNTNTPGVIVTGV- 292
            :..:|....|.||.:        ..:|...|:.....::.|:.|     |.|..|||......:
Mouse   491 TTQNNKPQVGEGTKERLENSDSSTTETTAVQVLSEPMEKADITNHVFATSAVGVNTPAEASPALS 555

  Fly   293 -------------------GNVGSDMCG--------QNHVGSQVGNDDP---------AACQMDM 321
                               |...||...        :|.|   :|..:|         .|..:|:
Mouse   556 SEEIPTEKPGMETQERGCEGGTTSDQSSPVLPAAAIENKV---LGGQEPDTSIAGFCKTASPLDL 617

  Fly   322 DL------------------------------SKSE-GTQAGGGLHQSVGTCTSSSK----GTGT 351
            .:                              |.:| ||.:....||. .|.|||.:    |:|.
Mouse   618 TMPGPSSDGMPEQNSESHARPAQSLSGQALLCSTAEAGTPSAEATHQP-STVTSSGRLEECGSGK 681

  Fly   352 RN---------------------KDFGDDMTRDAHSHDMHQHNVIN----------NNTRRKTKS 385
            .:                     ....|.:|.|...:......|.:          |..::.|..
Mouse   682 ASLPESTMVQPSTQELCTTLCPEDPQADTVTSDTVKNTQKSVGVCHLCVSDAKNQGNGLKQDTPL 746

  Fly   386 T---EDM----NVDTSTLKRM----LKPMPSTESPVTSPEMGRRRYNYYNANAAQTLGHPPHMHQ 439
            |   ||:    :|.:.|.|.:    :.|..|:.|..:|||.       .:......|...|...:
Mouse   747 TNVLEDVPRLPSVVSQTEKELAPDQVSPPASSFSLASSPES-------ESVTKDDALSLVPSQKE 804

  Fly   440 HGMAMGMGGGGGGGHHIMNNNTMGRASSQSSRFSGSRSSHEIGRGY--PPRNLYLELERERSCI- 501
            .|.|...          ::..|..|........|   .:.::|.|.  ||.:..:||.......
Mouse   805 KGTATPQ----------LHRTTACRDGPDGRDLS---DTDKVGDGATDPPPSSAVELRTSMGNTS 856

  Fly   502 --------EGSPP---------------------SDNVMFDNQCYATTPSSSN--GNSDQDQSYG 535
                    |||.|                     ||..:.:.......|.||.  |...:|::..
Mouse   857 PVGIGGEQEGSSPTATLEVLSDSLLHNVDKAALVSDFTLPEEGVSVVVPESSTALGQDGKDRAMS 921

  Fly   536 ------QQQSSGQHPQQQQGPPQ----------------RSSRHQHH-------------HQQAP 565
                  ...||....:.|:.||.                ...:.|.|             .:.||
Mouse   922 CSSVKEDVHSSEMSREDQRTPPSGQEIPGLCEKPMSALCAEEKAQQHTPSACLKTETKDIKEVAP 986

  Fly   566 NVT-PTPGS-------PTSRLLLEYEMHLRNT-------LAKGMDAESYSLH------------- 602
            .|: .|.|.       |.:.|..:.:....:|       |..|:...|.:||
Mouse   987 QVSLLTEGGAAKSLVPPRTSLSADSKQKASSTEQSGSSLLPSGLPGASEALHCNQPSALDVVVEN 1051

  Fly   603 ------TFEALLSQS-MENLEFAESLPGSNQRSPYPLRRPNAKSSTLPLPPHRPLSTIRDKER-- 658
                  |....:|:| ||::..|::.|.:.:.     |:.:|......:    |:|.:.::|:
Mouse  1052 TQFQGETNACEVSRSAMEDVTVADASPATAEP-----RKKDASHCIKDI----PISELLNQEKQM 1107

  Fly   659 -----DRDRDGYYSDRNELIR------ERERERDRGYLSDHNSSFSNSR---CASCIGESARAQW 709
                 :...|...:|..|:|.      :.:||...|  :|.|.:.|||:   ....:...||
Mouse  1108 TPSLPEAFLDKGVTDLQEVITPEIEPLDCKRETLEG--TDLNCATSNSKETPIEKPMQPLAR--- 1167

  Fly   710 FRHSDGWRSGSSTIGSGSGHGMMTQQIPGSGHKRSPWDSLPSLRQDSSLNDSGYKSA----RADS 770
                        .:.:.:|..::...:|.:..|:....|:|:  ::|:......::|    ||||
Mouse  1168 ------------DLPTETGLSVINNNVPQADMKQVAQASIPA--EESNATTVSTQAADVPTRADS 1218

  Fly   771 LEQRA-----EFIRQ---------------DSLRSEYLSDRESRYG------------------- 796
            :|:.|     ..|||               .||.|......|:.|.
Mouse  1219 IEETATRIVEAVIRQVRASNALMAKVETQNPSLSSPETKQLENAYTESACAFLPGETPQIEKTHE 1283

  Fly   797 ----------------IVQQASIESTDSRMCYLTSSEISDDDRMSLTTAVSDEDDGESVMASPYK 845
                            |:.:::.|..|:|    |..|:   |.:|..:|.|:|:...:..|:|..
Mouse  1284 DTTGQCGAETEEPEKIILPESAPEMPDTR----TGDEV---DLLSRISAASEEEAVGNGAATPKM 1341

  Fly   846 AKATGTAASSFNCTGAVRKAGFLSVKKWLLRKKHQIELARKRGWKGYWVCLKGTTLLF------- 903
            .:..||.|:  :||..:...    .::...|.|...::.|: .|.....|.:..:||.
Mouse  1342 KQGPGTQAN--DCTRVIPSC----CEEKNQRNKSSPKINRE-SWCAIEPCPEAASLLASKQSSEC 1399

  Fly   904 ---------YPCDSREG--RSVEAAPKHLIIVDGAIMQPI--PEHPKRDYIFCLSTAF------- 948
                     ..|.|:||  :.|..:...|.......|..|  |: |:.:.:.|.:|..
Mouse  1400 RSFIDVGLGTECASKEGMLQRVSGSESDLFHSPSDEMDSIIFPK-PEEEQLLCDTTGSSSSTDDT 1463

  Fly   949 ----------GDAYL----------------------FQAPCQVELE--------------NWVN 967
                      .|..|                      .:||...|.|              :.:.
Mouse  1464 ASLDRHSSHGSDVSLPQTSKLNRSRNHQSANGFFSPGVEAPESRESESEPAGSGEMEEEEMDSIT 1528

  Fly   968 SIHSACA---------AAFARHR---GKT----------GTLHLLQEEIFR-------------- 996
            .:.:.|:         :.|.||.   ||.          .::..|...:.|
Mouse  1529 EVPANCSFLRSSMRSLSPFRRHSWGPGKNAASDAEMNQRSSMRALGHVVRRPPIHRRSMSWCPSG 1593

  Fly   997 --LEKAIESDHKL---------------KHMAELQQSVVTDQETRH------QIQTQILQWEENL 1038
              ...|:.:|..:               |..:.|:.|.....|.|:      |..:.:...||:|
Mouse  1594 VQYSAALNADFNIRSFSLEGLTGGGVGNKPSSSLEMSSANSSELRNPFGGEEQRNSLMSLSEEHL 1658

  Fly  1039 E---RLHCEQF-RLRCYMASLQ---------SGELPNPKSLLTHVSRPTKNTLNKLGVFTVSSFH 1090
            |   |.|...| :..||.:..|         |..|....||:| :|.|        |:.:...||
Mouse  1659 EPDQRQHHRMFDQQTCYRSKQQGFNYCTSAISSPLTKSISLMT-ISHP--------GLDSSRPFH 1714

  Fly  1091 AFICARSPSLLN---NLLAGRGATKRRPPMLSRSNSGSSRRSMQMNSRDEPEKTFKVAMPDNAYS 1152
            :.....:.|:..   |.|         ||       ..|:::.:..|..:..:||.
Mouse  1715 STSANLTESITEENCNFL---------PP-------SPSKKNFEEKSGTKVSRTFS--------- 1754

  Fly  1153 TVYLRDAMSVEEFLASACARRNLNPMEHFVRVK-KRRDMEDHNYFVPHRNDLIENYLHNHEFVEV 1216
              |:|:.||        .::::....:...::| |.:|.::        .:..:..|:.|.|..:
Mouse  1755 --YIRNKMS--------SSKKSKEKEKEKDKIKEKEKDSKE--------KEKDKKTLNGHTFSPI 1801

  Fly  1217 ----------CMKILYQVELQRTTLEQMWGFSVEAELIENAERQDELCCYVSRVEDKSVAMHNGI 1271
                      |||..                          ..:|...|     ......:|.|.
Mouse  1802 PIVGPISCSQCMKPF--------------------------TNKDAYTC-----AGCGAFVHKGC 1835

  Fly  1272 IKGDEIMVINGAIVSDLDMMYLESVLQEEQSLSM---MMRSSRTEPPDLVGIMRVTDDMIDSLVC 1333
            .:       |.|..:.:.|...:..||...:.|:   :||:..::|.:.   .|....:.|....
Mouse  1836 RE-------NLASCAKVKMKQPKGSLQAHDTSSLPTVIMRNKSSQPKER---PRSAVLLADEATA 1890

  Fly  1334 PPPPTD---------PPVMSEEMITGLIVPAPGWNGTSKDLYSPEAESSPATSFVDPAAMAAQ-- 1387
            .|..|:         ...:|.:.|||:        |..:::.:.....|.:|..::......:
Mouse  1891 APMFTNRRSQQSVSLSKSVSIQNITGV--------GNDENMSNTWKFLSHSTDSLNKICKVNEST 1947

  Fly  1388 --LAVGGVGGVVVGGLGVAKPTSRTSSFEIENLLKTAEQETRKSSPTGSVTSSVSTTALTPSRQL 1450
              |...|||..:..|..:....|.:...|.|:..:|.:.:..|                   :|.
Mouse  1948 ESLTDEGVGTDMNEGQLMGDFESDSKQLEAESWSRTVDSKFLK-------------------QQK 1993

  Fly  1451 TDAEKLRKVVMELVDTERTYVKHLNNLLEHYLEPMKRETFLSNAEINALFGNIHEIVTFQRQFLQ 1515
            .|..|.::|:.||:.||..:::.|..:.:.|...|..:.......:..||..:.|:::...||.|
Mouse  1994 KDVVKRQEVIYELMQTELHHIRTLKIMSDVYSRGMMTDLLFEQQMVEKLFPCLDELISIHSQFFQ 2058

  Fly  1516 NL----EESLDLEPDFNKFEHCGQFRNVLF-AIGSAFLYYVNHF---------KLYSSFCASHSK 1566
            .:    :|||     .:|.|     :|.|. .||...   |:.|         |.|..||..|::
Mouse  2059 RILERKKESL-----VDKSE-----KNFLIKRIGDVL---VSQFSGESAERLKKTYGKFCGQHNQ 2110

  Fly  1567 AQ---KVLHPNEGNHALQEFLAARNPKQQHSSTLESYLIKP------IQRILKYPLLLQQMRNLT 1622
            :.   |.|:..:  ...|.|:     |::.||::...|..|      .|||.|||:|.|::...|
Mouse  2111 SVNYFKDLYTKD--KRFQAFV-----KKKMSSSVVRRLGIPECILLVTQRITKYPVLFQRILQCT 2168

  Fly  1623 DTRADEHVHLCEALKGMEKVAEHINEMQRIHEEYGAIFDHLFRQHQKSCKQPIDLSPGDLLYYGG 1687
            .....|...|.::|..::.|             .||: |.....::|..:
Mouse  2169 KDNEVEQEDLTQSLSLVKDV-------------IGAV-DSKVASYEKKVR--------------- 2204

  Fly  1688 VEWLNISDFLGKIKKGLELHAMCFVFKSAVVFLCKERLRQKKKLM--GVSSKNATNEVEIIRYQV 1750
                     ||:|....:..:: ...||..:| .||.||:||.:.  .|..|:.|..::.::..:
Mouse  2205 ---------LGEIYTKTDSKSI-MRMKSGQMF-AKEDLRRKKLVRDGSVFLKSTTGRLKEVQAVL 2258

  Fly  1751 LIPVTEVQVRASSAKDMDSHFLWELIHLRSQLQRRSEKVYVLSNSTADFRNAFLKTIRQIIRESV 1815
            |   |::.|                     .||.:.:| ||.  ::.|.::..:...:.|:||
Mouse  2259 L---TDILV---------------------FLQEKDQK-YVF--ASLDHKSTVISLKKLIVRE-- 2294

  Fly  1816 RNMSIPMKNFGGSSGSVSGHSSQGMG--SMGYPGNSQTLERPKQQITIVHGSHTLGKPKKKSGSQ 1878
                             ..|..:|:.  |||       ::.|  ::..||.|         |..:
Mouse  2295 -----------------VAHEEKGLFLISMG-------VKDP--EMVEVHAS---------SREE 2324

  Fly  1879 RHSAGNIDYDNLSGSQEADD--LPPSVGVVHYASGHTHGQQMQPAGFRGRSKTVGDVTEITCSSP 1941
            |:|...|..|.::.....:|  :|                          |:...:...:...:.
Mouse  2325 RNSWIQIIQDTINSLNRDEDEGIP--------------------------SENEEEKKLLDTKAR 2363

  Fly  1942 EPHQQQQQQQQQQQQLMQQGHAHAHPHPH---PHPREPPPPPIRQPHLHHHSSDIERIDPGTKSE 2003
            |..:|.||:.||...|:::..........   |.|.:..|.         ||..:.     .:|.
Mouse  2364 ELKEQLQQKDQQILLLLEEKEMIFRDMTECSTPLPEDCSPT---------HSPRVL-----FRSN 2414

  Fly  2004 GEEDSQQGTIRPKATLGRTPNHLTLSTTSTLSVGSTGSQARLIQSSHPPASYQ----PVLM---- 2060
            .||..:.|.:...|.     |.:.:  ..:|..||.|  ..|.||...|...:    |:.:
Mouse  2415 TEEALKGGPLMKSAI-----NEVEI--LQSLVSGSLG--GTLGQSISSPVEQEVMAAPISLPRRA 2470

  Fly  2061 ------------------KDLGKPSPDESELESESEVHAAQVAASFNLSLGSSGSLFASDARDNQ 2107
                              |:.|....|....||:|           .|..|.:|:|.....|:::
Mouse  2471 ETFGGFDCHQMNASKGGEKEEGDDGQDLRRTESDS-----------GLKKGGNGNLVFMLKRNSE 2524

  Fly  2108 TQSRTQSQIHSLHPSPRQSPRQSPRQSPKQDIEVIE------------IFESEAERLAASQQVAV 2160
            ...::...:|.|....:....|.......|.:.:.|            :.|.|.:|....|:..:
Mouse  2525 QVVQSIVHLHELLSMLQGVVLQQDSYIEDQKLVLTEKVLTRSASRPSSLIEQEKQRSLEKQRQDL 2589

  Fly  2161 VH------QHPSDQAR-----ESGHKQRRDRDRNPTETDATARFMDKHLS-DLEQENLTDGT--C 2211
            .:      ||..::.|     |:..::.|||:....|.:.|.|...:.|. |.|:.....||  |
Mouse  2590 ANLQKQQAQHLEEKRRREREWEAREQELRDREAKLAEREETVRRRQQDLERDREELQQKKGTYQC 2654

  Fly  2212 DIVAYMESLREKAFDPQDLAEDTG--DHQQNDSSLSPEPQTIVENTQQTVVVDIESPSRPATESD 2274
            |:.....:.::...:.:.|..||.  ..:|.|.:|..     |.|....:   :..||. ...||
Mouse  2655 DLERLRAAQKQLEREQEQLRRDTERLSQRQMDQNLCQ-----VSNKHGRL---MRIPSF-LPNSD 2710

  Fly  2275 STGNTTGPS-ERSGDETEEEATTSSLASHIVVIDKK--VDKIFKAKSP----ERPASTKVKGGRR 2332
            ...:.:.|| .:||          ||.|.:.|..|:  :.:..|.|.|    ...:.|||..|:.
Mouse  2711 EFSSPSAPSVTKSG----------SLDSELSVSPKRNSISRTQKDKGPFHILGSASQTKVPEGQS 2765

  Fly  2333 QIYISPDRCKSQGSSPVRIIKIKSPRTSLSDQGKRLSVCSSRDNSQDRLSAGRRT-----PSPRR 2392
            |       ..|..|:..|:..:..|:.....:.|               |.|.||     |:...
Mouse  2766 Q-------APSSTSTSTRLFGLSKPKEKKEKKKK---------------SKGSRTQPGDGPASEV 2808

  Fly  2393 SSEG 2396
            .:||
Mouse  2809 PAEG 2812

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674	22/109 (20%)
		PH1_Tiam1_2	860..985	CDD:269937	31/219 (14%)
		PH	873..974	CDD:278594	25/173 (14%)
		TIAM1_RBD	1141..1219	CDD:176413	13/88 (15%)
		PDZ_signaling	1221..1308	CDD:238492	10/89 (11%)
		RhoGEF	1456..1647	CDD:238091	52/213 (24%)
		PH2_Tiam1_2	1650..1823	CDD:269957	31/174 (18%)
		PH	1693..1812	CDD:278594	25/120 (21%)
Akap13	XP_036009413.1	opacity_OapA	<1150..>1298	CDD:411470	26/164 (16%)
		C1_AKAP13	1792..1848	CDD:410428	12/93 (13%)
		RhoGEF	1999..2193	CDD:238091	54/227 (24%)
		PH_AKAP13	2236..2338	CDD:275427	29/165 (18%)
		SMC_prok_B	<2519..2674	CDD:274008	27/154 (18%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	0.910