DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sif and Plekhg1

DIOPT Version :9

Sequence 1:	NP_001261446.1	Gene:	sif / 43892	FlyBaseID:	FBgn0085447	Length:	2734	Species:	Drosophila melanogaster
Sequence 2:	NP_001177928.1	Gene:	Plekhg1 / 679812	RGDID:	1583676	Length:	1380	Species:	Rattus norvegicus

Alignment Length:	1457	Identity:	301/1457 - (20%)
Similarity:	475/1457 - (32%)	Gaps:	503/1457 - (34%)

- Green bases have known domain annotations that are detailed below.

  Fly  1406 PTSRTSSFEIE--------NLLKTAEQETRKSSP----TGSVTSSVSTTALTPSRQLTDAEKLRK 1458
            |....||.|::        |:....|::.|  :|    |.....:.:.:|.:|.....|     :
  Rat    58 PAQPFSSSELQKDNPAGPPNMDGVGEKQPR--APWRVDTNGTPQTTADSATSPKLLYVD-----R 115

  Fly  1459 VVMELVDTERTYVKHLNNLLEHYLEPMKRETF--LSNAEINALFGNIHEIVTFQRQFLQNLEESL 1521
            ||.|:::||||||:.|.:::|.||..::.:|.  |...:..||||||.:|..|..:.||.|
  Rat   116 VVQEILETERTYVQDLKSIVEDYLACIRDQTKLPLGTEDRAALFGNIQDIYHFNSELLQEL---- 176

  Fly  1522 DLEPDFNKFEHCGQFRNVLFAIGSAFLYYVNHFKLYSSFCASHSKAQKVLHPNEGNHALQEFLAA 1586
                     |:|   .|...||...|:.....|.:|:.:|.::.::..||.....|..|.:|...
  Rat   177 ---------ENC---ENDPVAIAECFVSKSEEFHIYTQYCTNYPRSVAVLTECMRNKMLAKFFRE 229

  Fly  1587 RNPKQQHSSTLESYLIKPIQRILKYPLLLQQMRNLTDTRADEHVHLCEALKGMEKVAEHINEMQR 1651
            |....:||..|.|||:||:||||||.|||.::.|..|...:.:..:.:|:..|::||.|||:|:|
  Rat   230 RQETLKHSLPLGSYLLKPVQRILKYHLLLHEIENHLDKDTEGYDVVLDAIDTMQRVAWHINDMKR 294

  Fly  1652 IHEEYGAIFDHLFRQHQ-----KSCKQPIDLSPGDLLYYG--------GVEWLNISDFLGKIKKG 1703
            .||       |..|..:     .:.|.|...|.|:|:..|        ....|.:.|.|..|.|.
  Rat   295 KHE-------HAVRLQEIQSLLTNWKGPDLTSYGELVLEGTFRIQRAKNERTLFLLDKLLLITKK 352

  Fly  1704 LE------LHAMC------------------FVFKS-AVVFLCKERLRQKKKL------------ 1731
            .|      .|.:|                  |.:|| .:....:.:.:|:|:|
  Rat   353 REDTFTYKAHILCGNLMLVEVIPKEPLSFSVFHYKSPKLQHTVQAKSQQEKRLWILHLKRLILEN 417

  Fly  1732 ----MGVSSKNATNEVEIIRYQVLI--PVTEVQVRASSAKDMDSHFLWELIHLRSQLQRRSEKVY 1790
                :...:|.|..|::.|.:....  |..||:....||.        ..:..:|:...||.||.
  Rat   418 HAAKIPAKAKQAILEMDAIHHPGFCYDPKGEVKTPCGSAP--------HRLRRKSEPSSRSHKVL 474

  Fly  1791 VLSNSTADFRNAFLKTIRQIIRE---SVRNMSIPMK-----------NFGGSSGSVSG-----HS 1836
            ..|.:..|        |:::.||   |....::|.:           |.....|||.|     |.
  Rat   475 KTSETAQD--------IQKVSREESSSQLTSAVPAQRNRQPGNAAVLNMLRGGGSVRGLWMDHHI 531

  Fly  1837 SQG-MGSMGYPGNSQTLERPKQQITIVHGSHTLGKPK--KKSGSQR--------------HSAGN 1884
            .|. ..|...|..::..:...|.......|..|...:  :::.|.|              .|:|:
  Rat   532 QQPFFPSRRTPQENEDDDDDYQMFVPSFSSSDLNSTRLCEENTSSRPCSWHLGLIEPTEISSSGH 596

  Fly  1885 IDYDNLSGSQEADDLPPSVGV-----VHYASGHTHGQQMQPAGFRGRSKTVGDVTEITCSSPEPH 1944
            ......|.:.|::..||.|.:     ..|..|.......||.| :|.....|...|:|....:
  Rat   597 RIVRRASSAGESNACPPEVRLRDSDGSQYCPGREMQNSPQPGG-QGGMTPFGSSVELTIDDID-- 658

  Fly  1945 QQQQQQQQQQQQLMQQGHAHAHPHPHPHPREPPPPPIRQPHLHHHSS--DIERIDPGTKSEGEED 2007
                                                    |::.:.|  |: ::....:.|.|..
  Rat   659 ----------------------------------------HVYDNISFEDL-KLMVAKRDETESS 682

  Fly  2008 ---SQQGTIRPKAT-----LGRTPNHLTLSTTSTLSVGSTGSQARLIQSSHPPASYQPVLMKDLG 2064
               |.:.::|||:|     ..|..:|.|.|..|.....|:|.:|. .||.|.....:..:...:|
  Rat   683 FSKSSRDSVRPKSTPELAFSKRQASHSTSSLHSKKETASSGQEAS-NQSIHEHQEVEENIYDTIG 746

  Fly  2065 KPSPDESELESESEVHAAQVAASFNLSLGSSGSLFASDARDNQTQSRTQSQIHSLHPSPRQSPRQ 2129
            .|.|                 .|.|.                                 ::|..|
  Rat   747 LPDP-----------------PSLNF---------------------------------QRSSLQ 761

  Fly  2130 SPRQSPKQDIEV-------IEIFESEAERLAASQQVAVVHQHPSDQARESGHKQRRDRDRNPTET 2187
            .|::|....:|.       :..|.|: :||..|:.....||.|||                   |
  Rat   762 QPKRSTFLGLEADFACCDSLRPFVSQ-DRLQFSEDDISYHQGPSD-------------------T 806

  Fly  2188 D----------ATARFMDKHLSDLEQENLTDGTCDIVAYMESLREKAFD---------------- 2226
            |          ......||.|||...|.:.:...|:..|::...:|:.|
  Rat   807 DYLSLLYDSPRCNLPIADKTLSDKLSEEVDEIWNDLENYIKKNEDKSRDRLLAAFPVSKDDVPER 871

  Fly  2227 -----PQDLAEDTGDHQQNDSSLS-PEPQTIVENTQQTVV------------------------- 2260
                 .|:|:.|.|   :..|.|: |..||.:...:..||
  Rat   872 LYVESTQELSRDAG---RATSMLALPGSQTFLTPGESRVVRASRANCSLEDDLISTEGSFLSLNQ 933

  Fly  2261 -------------VDIESPSRPATESDSTGNTTGPSERSGDETEEEATTSSLASHIVVIDKKVDK 2312
                         .|:.|.|.|.|:.::      |..|.....:.::....:|.......|||::
  Rat   934 LSLVSDEPSEDNPYDLASCSLPQTDPEN------PDPRMEITDKTKSRVFMMARQYSQKIKKVNQ 992

  Fly  2313 IFKAKSP--ERPAST---------------KVKGG---------RRQIY---------------- 2335
            :.|.|||  |:|.|:               |.:||         ..|:|
  Rat   993 LLKVKSPELEQPLSSQHRPIHKDLVAILEEKRQGGPAIGARIAEYSQLYDQIVFRETPLKSQKDG 1057

  Fly  2336 ------------ISPDRCKSQG-------SSPVRIIKIKSPRTSLSDQGKRLSVCSSRDNSQDR- 2380
                        .||.:.:..|       |...|.:...||:|....:.|......|......|
  Rat  1058 WTSTQEPALHRPASPPQVQGAGEDWLWHSSYSNREVADFSPQTEQDSKSKYPVTLESITKIMPRQ 1122

  Fly  2381 LSAGRRTPS---------------------PRRSSEGGGILKHTTGPD--PGILKPPSSPAKSKS 2422
            |||....||                     |.:.:.|.|.|.::.|..  ...|:|.|.|..|.|
  Rat  1123 LSAACSVPSLQGSDPLLGSVQQRCSVVVSQPHKENSGQGHLYNSLGRKAISAKLQPYSRPQSSSS 1187

  Fly  2423 P--DRSCLKKGGPSHVCSVETLSPRVSPRGS---------MDHLSPDRGMTSSSCLRHSPR---- 2472
            .  ::|......||.....:.||.:.||||:         .|....|.|..|...|:.|.:
  Rat  1188 VLINKSLDSINYPSETEKKQLLSLQKSPRGASQQDLLSGLADSCQQDTGKQSDLTLQDSQKVLVV 1252

  Fly  2473 ----------------SSF-DSHGESDHNLDVPHGSRKRSMSAHGSFETGTRPRAQETYQYYPVY 2520
                            |:| ::.|:.|..:::        .|..........||......  |..
  Rat  1253 NRNLPLSAQIASQNYFSNFKETEGDEDDYVEI--------KSEEDEVRLDLSPRRNRKSD--PKI 1307

  Fly  2521 DNQTCSDH-------YVAAAP-TGRYG 2539
            .:..|||:       |....| :||.|
  Rat  1308 PDPDCSDNICRHNTPYSLKEPVSGRLG 1334

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937
		PH	873..974	CDD:278594
		TIAM1_RBD	1141..1219	CDD:176413
		PDZ_signaling	1221..1308	CDD:238492
		RhoGEF	1456..1647	CDD:238091	65/192 (34%)
		PH2_Tiam1_2	1650..1823	CDD:269957	46/231 (20%)
		PH	1693..1812	CDD:278594	30/161 (19%)
Plekhg1	NP_001177928.1	RhoGEF	116..291	CDD:279015	66/190 (35%)
Plekhg1	NP_001177928.1	PH_PLEKHG1_G2_G3	272..417	CDD:270063	33/151 (22%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	2	1.910