DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sif and PLEKHG2

DIOPT Version :9

Sequence 1:	NP_001261446.1	Gene:	sif / 43892	FlyBaseID:	FBgn0085447	Length:	2734	Species:	Drosophila melanogaster
Sequence 2:	XP_011525534.1	Gene:	PLEKHG2 / 64857	HGNCID:	29515	Length:	1387	Species:	Homo sapiens

Alignment Length:	1694	Identity:	338/1694 - (19%)
Similarity:	548/1694 - (32%)	Gaps:	589/1694 - (34%)

- Green bases have known domain annotations that are detailed below.

  Fly  1301 QSLSMMMRSSRTEPPDLVGIMRVTDDMIDSLVC------PPPPTDPPVMSEEMITGLIVPAPGWN 1359
            |.||:      ::|...:|..|..:......||      |..||              :.:|..:
Human     6 QGLSL------SKPSPSLGCGRRGEVCDCGTVCETRTAAPAAPT--------------MASPRGS 50

  Fly  1360 GTSKDLYSPEAESSPATSFVDPAAMAAQLAVGGVGGVVVGGLGVAKPTSRTSSFEIENLLKTAEQ 1424
            |:|..|.:..:|..||..                            ||...|:...|.|  ....
Human    51 GSSTSLSTVGSEGDPAPG----------------------------PTPACSASRPEPL--PGPP 85

  Fly  1425 ETRKSSPTGSVTSSVSTTALTPSRQLTDAEKLRKVVMELVDTERTYVKHLNNLLEHYLEPMKRET 1489
            .....||.|...|:      .|||       |.:|..|:|:|||.||:.|.:::|.||.|:....
Human    86 IRLHLSPVGIPGSA------RPSR-------LERVAREIVETERAYVRDLRSIVEDYLGPLLDGG 137

  Fly  1490 F--LSNAEINALFGNIHEIVTFQRQFLQNLEESLDLEPDFNKFEHCGQFRNVLFAIGSAFLYYVN 1552
            .  ||..::..||.||.:|..|..:.|::||.|          ...|       .|...|:....
Human   138 VLGLSVEQVGTLFANIEDIYEFSSELLEDLENS----------SSAG-------GIAECFVQRSE 185

  Fly  1553 HFKLYSSFCASHSKAQKVLHPNEGNHALQEFLAARNPKQQHSSTLESYLIKPIQRILKYPLLLQQ 1617
            .|.:|:.:|.::..:..:|.....:.....:|..|..:.:||..|:|:|:||:||||||.||||:
Human   186 DFDIYTLYCMNYPSSLALLRELSLSPPAALWLQERQAQLRHSLPLQSFLLKPVQRILKYHLLLQE 250

  Fly  1618 M-RNLTD---TRADEHVHLCEALKGMEKVAEHINEMQRIHEEYGAIFDHLFRQHQKSCKQPIDLS 1678
            : ::..:   |...|.|.  ||:..|..||.:||:|:| .:|:.|....:  |.:.......:||
Human   251 LGKHWAEGPGTGGREMVE--EAIVSMTAVAWYINDMKR-KQEHAARLQEV--QRRLGGWTGPELS 310

  Fly  1679 P-GDLLYYGGVE----------------WLNISDFLGKIKKGLELHAMCFVFKSAVVFLCKERLR 1726
            . |:|:..|...                :|.....|...::|||     :.:|.. :|.|...:.
Human   311 AFGELVLEGAFRGGGGGGPRLRGGERLLFLFSRMLLVAKRRGLE-----YTYKGH-IFCCNLSVS 369

  Fly  1727 QK-KKLMGVSSKNATNEVEIIRYQVLIPVTEVQVRASSAKDMDSHFLWELIHLRSQLQRRSEKVY 1790
            :. :..:|..    .:::.|.:::.|:          .||:.:...||  ||...:|...:....
Human   370 ESPRDPLGFK----VSDLTIPKHRHLL----------QAKNQEEKRLW--IHCLQRLFFENHPAS 418

  Fly  1791 VLSNSTADFRNAFLKTIRQIIRES-----------VRNMSIPMKNFGGSSGSVSGHSSQGMGSMG 1844
            :.:.:            :|::.|:           :..::.|:       ||.....::..
Human   419 IPAKA------------KQVLLENSLHCAPKSKPVLEPLTPPL-------GSPRPRDARSF---- 460

  Fly  1845 YPGNSQT--------LERPKQQITIVHGSHTL----GKPK-KKSGSQRHSAGNIDYDNLSGSQEA 1896
            .||...|        :.|.::|...|...:.:    .||. |.:||:...........:|||...
Human   461 TPGRRNTAPSPGPSVIRRGRRQSEPVKDPYVMFPQNAKPGFKHAGSEGELYPPESQPPVSGSAPP 525

  Fly  1897 DDL----PPSVGVVHYASGHTHGQQMQPAG----------------------------FRGRSKT 1929
            :||    ||:               :.|:|                            |.|.|:.
Human   526 EDLEDAGPPT---------------LDPSGTSITEEILELLNQRGLRDPGPSTHDIPKFPGDSQV 575

  Fly  1930 VGDVTEITCSSPEPHQQQQQQQQQQQ--QLMQQGHAHAHPHPHP-HPRE----------PPPPPI 1981
            .||...:|..:.......::::::::  ::.::|       |.| |..|          |..|.:
Human   576 PGDSETLTFQALPSRDSSEEEEEEEEGLEMDERG-------PSPLHVLEGLESSIAAEMPSIPCL 633

  Fly  1982 RQ----PHLHHHSSDIERIDPGTKSEGEEDSQQGTIRP-KATLGRTPNHLTLSTTSTLSVGSTGS 2041
            .:    |:|....|..| |..|::.....|.......| ...:...||..:||:|.|||..| ..
Human   634 TKIPDVPNLPEIPSRCE-IPEGSRLPSLSDISDVFEMPCLPAIPSVPNTPSLSSTPTLSCDS-WL 696

  Fly  2042 QARLIQSSHPPASYQPVLMKD----LGKPS------------------------------PDESE 2072
            |..|.:.:..||:.:.:....    ||:|.                              |||..
Human   697 QGPLQEPAEAPATRRELFSGSNPGKLGEPPSGGKAGPEEDEEGVSFTDFQPQDVTQHQGFPDELA 761

  Fly  2073 LESESEVHAA-------QVA-ASFNLSL---------GSSGSLFASDARDNQTQSRTQ------- 2113
            ..|.||:.:|       |:| ..|...|         |.|||..|......:|.||.:
Human   762 FRSCSEIRSAWQALEQGQLARPGFPEPLLILEDSDLGGDSGSGKAGAPSSERTASRVRELARLYS 826

  Fly  2114 SQIHSLHPSPRQSPRQSPRQSPK-----QDIEVIEIFESEAERLAASQQVAVVH-QHPSDQARES 2172
            .:|..:..:..::...:||:.|:     |....:...::|...|.|...|.|.. ..|...|:||
Human   827 ERIQQMQRAETRASANAPRRRPRVLAQPQPSPCLPQEQAEPGLLPAFGHVLVCELAFPLTCAQES 891

  Fly  2173 G--------------HKQRRDRDRNPTETDATARFMDKHLSDLEQENL-------------TDGT 2210
            .              .||....|.           ...|:|:|.:::|             ..|:
Human   892 VPLGPAVWVQAAIPLSKQGGSPDG-----------QGLHVSNLPKQDLPGIHVSAATLLPEQGGS 945

  Fly  2211 CDIVAYMESLREKAFDPQDL---AEDTGDHQQNDSSLSPEPQTIVENTQQTVV------------ 2260
            ..:.|...:...|...|..|   |..|...|.:.....|....:.|:....|:
Human   946 RHVQAPAATPLPKQEGPLHLQVPALTTFSDQGHPEIQVPATTPLPEHRSHMVIPAPSTAFCPEQG 1010

  Fly  2261 --VDIESPSRPATE----SDSTGNTTGPSERSGDETEEEATTSSLASHIVVIDKKVDKIFKAKSP 2319
              .||..|:.||..    ||.|.:.|.|..:.....:.|                        ||
Human  1011 HCADIHVPTTPALPKEICSDFTVSVTTPVPKQEGHLDSE------------------------SP 1051

  Fly  2320 ERPASTKVKGGRRQIYISPDRCKSQ--------GSS-------------PVRIIKIKSPRTS-LS 2362
            .....|| :||.|.:. .||...||        |||             |..:..::.|.|| |.
Human  1052 TNIPLTK-QGGSRDVQ-GPDPVCSQPIQPLSWHGSSLDPQGPGDTLPPLPCHLPDLQIPGTSPLP 1114

  Fly  2363 DQGKRLSVCSSRDNSQDRLSAGRRTPSPRRSSEGGGILKHTTGPDPGILKP------PSSPAKSK 2421
            ..|..|   ..|..:...||..:..|..:       :...|..|.|.:|..      |:||.:..
Human  1115 AHGSHL---DHRIPANAPLSLSQELPDTQ-------VPATTPLPLPQVLTDIWVQALPTSPKQGS 1169

  Fly  2422 SPDRSCLKKGGPSHV----------CSVETLSPRVSPRGSMD-HLS-----PDRG---------- 2460
            .||..     ||:..          ..|:.|:|.:..:..:| |:.     |:||
Human  1170 LPDIQ-----GPAAAPPLPEPSLTDTQVQKLTPSLEQKSLIDAHVPAATPLPERGGSLDIQGLSP 1229

  Fly  2461 -MTSSSCLRHSPRSSFDSHG---ESDHNLDVPHGSRKRSMSAHGSFETGTRPRAQETYQYYPVYD 2521
             ...::.:...|..|..||.   ||. :|..||.....|....|                    .
Human  1230 TPVQTTMVLSKPGGSLASHVARLESS-DLTPPHSPPPSSRQLLG--------------------P 1273

  Fly  2522 NQTCSDHYVAAAPTGRYGGGTSSRSRLSKSLERSTSRDSTTTSSSYGHRAYGVSPDRNYVVFSSG 2586
            |......|:||             |.:|:||.|.........::|.|.             :||.
Human  1274 NAAALSRYLAA-------------SYISQSLARRQGPGGGAPAASRGS-------------WSSA 1312

  Fly  2587 PLRSQSAENTFSSQPIYDPLPRHPPQPLHQPPMHHQQQQQQQQQQQYMSYAPEMGGYTGCQHDPQ 2651
            |       .:.:|.|        ||||...||           ..:.:|||..:..:.|
Human  1313 P-------TSRASSP--------PPQPQPPPP-----------PARRLSYATTVNIHVG------ 1345

  Fly  2652 GGYQLSSSAPEYTTCIDCLYQKRPSXHKSRVRQSRTRKKTPRGMVVVSAQPTATGGIGFVPEMGS 2716
            ||.:|                 ||: .:.|:........|...|.:..||             |:
Human  1346 GGGRL-----------------RPAKAQVRLNHPALLASTQESMGLHRAQ-------------GA 1380

  Fly  2717 SEAP 2720
            .:||
Human  1381 PDAP 1384

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937
		PH	873..974	CDD:278594
		TIAM1_RBD	1141..1219	CDD:176413
		PDZ_signaling	1221..1308	CDD:238492	3/6 (50%)
		RhoGEF	1456..1647	CDD:238091	60/196 (31%)
		PH2_Tiam1_2	1650..1823	CDD:269957	30/201 (15%)
		PH	1693..1812	CDD:278594	18/119 (15%)
PLEKHG2	XP_011525534.1	RhoGEF	107..283	CDD:279015	60/194 (31%)
		PH_PLEKHG1_G2_G3	264..414	CDD:270063	37/176 (21%)
		PH	<338..410	CDD:278594	17/93 (18%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1291
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	2	1.940