DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sif and arhgef25b

DIOPT Version :9

Sequence 1:	NP_001261446.1	Gene:	sif / 43892	FlyBaseID:	FBgn0085447	Length:	2734	Species:	Drosophila melanogaster
Sequence 2:	XP_005166148.2	Gene:	arhgef25b / 569561	ZFINID:	ZDB-GENE-080225-17	Length:	625	Species:	Danio rerio

Alignment Length:	633	Identity:	133/633 - (21%)
Similarity:	257/633 - (40%)	Gaps:	139/633 - (21%)

- Green bases have known domain annotations that are detailed below.

  Fly  1293 LESVLQEEQSLSMMMRSSRTEPPDLVGIMRV-TDDMIDSLVCPPPPTDPPVMSEEMI----TGLI 1352
            ||.:.:::.|.::...|..:.|||......| |||:     .|..|::..:.|....    |..|
Zfish    67 LERIPEDKDSYTLTGLSDVSLPPDDPPSASVSTDDL-----SPISPSNHQLFSSRRASVTSTRSI 126

  Fly  1353 VPAPGW---------------NGTSKDLYSPEAES------SPATSFVDPAAMAAQLAVGGVGGV 1396
            .....|               :.:||.:.|||..:      |||.|.:.|...:.:| ......:
Zfish   127 KAFKRWLSSPVRRLSLGGGNKSQSSKGIQSPEGSTYMFLPLSPAQSPLSPMEKSQKL-TRSYRSL 190

  Fly  1397 VVGGLGVAKPTSRTSSF---EIENLLKTAEQETRKSSPTGSVTSSVSTTALTPSRQLTDAEKLRK 1458
            ..|.|  ::.||..|.:   .:.:|.:..:....::|.:.|:|..   |.:..|:.|.|.|:.|:
Zfish   191 DCGEL--SRETSPDSPYYPRYLNDLFQRNQSFDVQTSVSPSLTDK---TLVEQSQSLEDNEQHRR 250

  Fly  1459 --------VVMELVDTERTYVKHLNNLLEHYLEPMKR---ETFLSNAEINALFGNIHEIVTFQRQ 1512
                    |:.|||:||:.||:.|..::|.|:..|:.   ..:|...: ..:|||||:|..:.:.
Zfish   251 TALEKSIYVLTELVETEKLYVEDLGLVVEGYMLTMRSVGVPEYLEGKD-KIVFGNIHQIYDWHKD 314

  Fly  1513 -FLQNLEESLDLEPDFNKFEHCGQFRNVLFAIGSAFLYYVNHFKLYSSFCASHSKAQKVLHPNEG 1576
             ||..||:.:. |||.               :...|:.:.....:|..:|.:..|::
Zfish   315 YFLGELEKCVS-EPDL---------------LAQLFIKHERRLNMYVVYCQNMPKSE-------- 355

  Fly  1577 NHALQEFLAA--RNPKQQ--HSSTLESYLIKPIQRILKYPLLLQQMRNLTDTRADEHVHLCEALK 1637
             |.:.|::..  .:.:||  |...|...||||:|||:||.|||:...........:...|..|::
Zfish   356 -HIVSEYIETYFEDLRQQLGHRLQLNDLLIKPVQRIMKYQLLLKDFLKYYTKAGRQTEDLERAVE 419

  Fly  1638 GMEKVAEHINEMQRIHEEYGAIFDHLFRQHQKSCKQ----------PIDLSPGDLLYYGGVEWLN 1692
            .|..|.:..|:|..:....|  |:...|...|..:|          .|...|.:...:...:.:.
Zfish   420 VMCFVPKRCNDMMNVGRLQG--FEGQLRAQGKLLQQDTFTFIENENSILSRPKERRVFLFEQLII 482

  Fly  1693 ISDFLGKIKKGLELHAMCFVFKSAVVFLC---KERLRQKKKLMGVSSKNATNEVEIIRYQVLIPV 1754
            :|:.:.: |:|..|..  :|:|:::...|   ::|..:....:.::|:|....::    :.::..
Zfish   483 LSEPMDR-KRGFSLPG--YVYKNSIKISCLSIEDRCPEDPCRLVLTSRNIDGSLQ----KFILRA 540

  Fly  1755 TEVQVRASSAKDMDSHFLWELIHLRSQLQRRSEKVYVLSNSTADFRNAFLKTIRQIIRESVRNMS 1819
            :..::|.|.|.|                      :..:..:..:|.||....| :..|:.::::|
Zfish   541 SSSEIRTSWAND----------------------IIQMLETQRNFLNALQSPI-EFQRKEIKSLS 582

  Fly  1820 IPMKNFGGSSGSVSGHSSQGMGSMGYPGNSQTLERPKQQITIVHGSHT 1867
            :      |.|.:.|...:.|:    .|.:|.:::|  |.:..:...|:
Zfish   583 L------GKSMTASASLNSGL----RPYSSASIDR--QTLPCLQSWHS 618

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937
		PH	873..974	CDD:278594
		TIAM1_RBD	1141..1219	CDD:176413
		PDZ_signaling	1221..1308	CDD:238492	3/14 (21%)
		RhoGEF	1456..1647	CDD:238091	53/206 (26%)
		PH2_Tiam1_2	1650..1823	CDD:269957	27/185 (15%)
		PH	1693..1812	CDD:278594	18/121 (15%)
arhgef25b	XP_005166148.2	RhoGEF	258..429	CDD:238091	52/196 (27%)
arhgef25b	XP_005166148.2	PH-like	434..573	CDD:327399	25/170 (15%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	1	0.910