DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sif and mcf2lb

DIOPT Version :9

Sequence 1:	NP_001261446.1	Gene:	sif / 43892	FlyBaseID:	FBgn0085447	Length:	2734	Species:	Drosophila melanogaster
Sequence 2:	XP_009303050.1	Gene:	mcf2lb / 569231	ZFINID:	ZDB-GENE-081104-416	Length:	1097	Species:	Danio rerio

Alignment Length:	1340	Identity:	253/1340 - (18%)
Similarity:	445/1340 - (33%)	Gaps:	447/1340 - (33%)

- Green bases have known domain annotations that are detailed below.

  Fly   808 SRMCYLTSSEISDDDRMSLTTA-VSDE-------------DDGESVMASP------------YKA 846
            ||.|....:||...:...|..| |..|             .||..::..|            ::.
Zfish    24 SRRCQWLQNEIMQKESSPLFAADVITELKRQFAFLSGGRAQDGSPIIIFPEYPSFCEIGDQEFRN 88

  Fly   847 KATG-TAASSFNCTGAVRKAGFLSVKKWLLRKKHQIELARKRGWKGYWVCLKGTTLL---FYPCD 907
            ..|. |:..|.:..|    .||:.|          |:..:.|     |.|||||.|.   ::|.:
Zfish    89 VLTYLTSIPSLSAAG----VGFIVV----------IDRRQDR-----WTCLKGTLLRISGWFPAN 134

  Fly   908 SREGRSVEAAPKHLIIV--DGAIMQPIPEHPKRDYIFCLSTAFGDAYLFQAPCQVELENWVNSIH 970
            .|           |::|  ..||:|.           .||..|
Zfish   135 LR-----------LVLVLRPSAILQR-----------TLSDVF---------------------- 155

  Fly   971 SACAAAFARHRGKTGTLHLLQEEIFRLEKAIESDHKLKHMAELQQSVVTDQETRHQIQTQILQWE 1035
                  |..||.......::...:..|...||.:       :|.|.:...|...|:      .|.
Zfish   156 ------FKLHRDDFKVPVIMLSSVADLHTYIERN-------QLTQELGGSQYYCHK------TWI 201

  Fly  1036 ENLERLHCEQF---------RLRCYMASLQSGELPN----PKSLL-THVSRPTKNTLNKLGVFTV 1086
            .:  |...|.|         ||:.:...|...|||:    ..||| ||.||.        .:|..
Zfish   202 SH--RTDLESFAALVKRMAQRLQVFGRELAETELPSNHLTASSLLNTHTSRK--------DIFKE 256

  Fly  1087 SSFHAFICARSPSLLNNLLAGRGATKRRPPMLSRSNSGSSRRSMQMNSRDEPEKTFKVAMPDNAY 1151
            ....|.  ::...:|.|:         |.|:                .||          ||::.
Zfish   257 DMAGAL--SQGRKILENI---------REPV----------------RRD----------PDSSL 284

  Fly  1152 STVYLRDAMSVEEFLASACARRNLNPMEHFVRVKKRRDMEDHNYFVPHRNDLIENYLHNHEFVEV 1216
            :...|.:..:|...|:      .||          ........:::.|:|.|           |:
Zfish   285 NPDQLENLATVHRLLS------QLN----------ESSTAFDEFWIQHQNKL-----------EL 322

  Fly  1217 CMKIL--------YQVELQRTTLEQMWGFS--------VEAELIENAERQDELCCYVSRVEDKSV 1265
            |:|:.        .:.||.|.| |.:..||        .|..|.|....:.::|..:.||. ..|
Zfish   323 CLKVCQFEHNFQQLRTELDRAT-ETLNAFSAVGISPAQTEHLLQELTNHEKKVCEVLDRVR-SLV 385

  Fly  1266 AMHNGIIKGDEIMVINGAIV---SDLDMMYLESVLQEEQSLSMMMRSSRTEPPDLVGIMRVTDDM 1327
            |...|:|  |...:::.:|.   |:|:        :..::|:..::..:|:....:.:.:..|.|
Zfish   386 AEGQGLI--DSSQILDDSIAAKCSELE--------KASENLTQELKDKQTKLTQAMDLHKRLDMM 440

  Fly  1328 I----DSL-VCPPPPTDPPVMSEEMITGLIVPAPGWNGTSKDLYSPEAESSPATSFVDPAAMAAQ 1387
            .    |.: :....|.| ...|:|                    ..|:..|....:::.|....:
Zfish   441 CKWCDDGIYMLASQPLD-KCQSQE--------------------GAESALSELECYLETANEKQK 484

  Fly  1388 LAVGGVGGVVVGGLGVAKPTSRTSSFEIE------------NLLKTAEQETRKSSPTGSVTSSVS 1440
            ..:..|.......|........|.:||.:            :|.|.|.::||...|......|:|
Zfish   485 WEISTVWQEYDNILNNELREQVTRAFEKKASLQEMFDRRRVSLKKLAAKQTRPVQPVAPSPESLS 549

  Fly  1441 TTAL---------------------------TPSRQLTDAEK------LRKVVM-ELVDTERTYV 1471
            :.|.                           ..||..:.:|:      ||:.|| ||::|||.||
Zfish   550 SPAHRDHESICISEDSDSRGSCKQINSDQVDRSSRNASVSEEEETLAVLRRHVMNELLETERAYV 614

  Fly  1472 KHLNNLLEHYLEPMKRETF-------LSNAEINALFGNIHEIVTF-QRQFLQNLEESLDLEPDFN 1528
            :.|..:|:.|...|...:.       |.|.: :.||||:.||..| :|.||:.||...|. |:.
Zfish   615 EELMCVLQGYAAEMDNPSMAPLIPAALQNKK-DVLFGNMQEIYNFHKRIFLRELETYTDY-PEL- 676

  Fly  1529 KFEHCGQFRNVLFAIGSAFLYYVNHFKLYSSFCASHSKAQKVLHPNEGNHALQEFLAARNPKQQH 1593
                          :|..||.::...::|..:|.:..:::.:..........||.    ..|.:|
Zfish   677 --------------VGRCFLDWMGELQIYEKYCHNKPRSESLWRQCSDCAFFQEC----QKKLEH 723

  Fly  1594 SSTLESYLIKPIQRILKYPLLLQQMRNLTDTRADEHVHLCEALKGMEKVAEHINEMQRIHEEYGA 1658
            ...|:|||:||:|||.||.||:::|...: ...:..|.|..||..:..:.:.:|:...:....|
Zfish   724 KLGLDSYLLKPVQRITKYQLLIKEMIKYS-KGCEGSVELQAALSSILGILKAVNDSMHLIAITG- 786

  Fly  1659 IFD---------------HLFRQHQKSCKQPIDLSPGDLLYYGGVEWLNISDFLGKIKKGLELHA 1708
             :|               .::.:|::                |.|:.:.::.|     |.::.|
Zfish   787 -YDGNLGDLGRLLMQGSFSVWAEHKR----------------GHVKVMELARF-----KPMQRH- 828

  Fly  1709 MCFVFKSAVVFLCKERLRQKKKLMGVSSKNATNEVEIIRYQVLIPVTEVQVRASSAKDMDSHF-L 1772
             .|:.:.|::| ||.|....:......|.:...|:.:    ..|.:||      .||.....| :
Zfish   829 -LFLHEKALLF-CKRREESGEGYEKAPSYSFKQELSM----AAIGITE------HAKGDSKKFEI 881

  Fly  1773 WELIHLRSQLQRRSEKVYVLSNSTADFRNAFLKTIRQI-----------IRESVRNMSIPMK--- 1823
            |         ....::||.:..::.:.:..::..||::           |.|..:|.:...:
Zfish   882 W---------SSSRDEVYTIQAASEEVKTIWVTEIRKLLTGQLEACKERIFEEPKNEASQQRAPE 937

  Fly  1824 NFGGSSGSVS---GHSSQGMGSMGYPGNSQTLERPKQQITIVHGSHTLGKPKKKSGSQRHSAGNI 1885
            .|...|.|.:   .:....:.|.|.....:..||.| ::..:..|.|         ::|....:.
Zfish   938 QFTSESSSTNRLVRNERSSLKSDGVEKQKREEERGK-ELESIFESRT---------AERAKGSSF 992

  Fly  1886 DYDNLSGSQE--ADDLP------PSVGVVHYASGHTHGQQMQPAGFRGRSKTVGDVTEITCSSPE 1942
            .::.....|:  :|..|      |::|.|.:.|..:..|..:....:|.......|......|.|
Zfish   993 SFETKPKRQDVRSDPTPLETGPHPNLGGVRWFSTSSLFQSRRRGWMKGSLSLDASVEHDGYFSAE 1057

  Fly  1943 PHQQQQQQQQQQQQLMQQGHAHAHPHPHPHPREPPPPPIR 1982
            .......:::::.:|                .|..|.|:|
Zfish  1058 EQANSDPEEEREDKL----------------SEEEPQPVR 1081

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937	26/129 (20%)
		PH	873..974	CDD:278594	19/105 (18%)
		TIAM1_RBD	1141..1219	CDD:176413	12/77 (16%)
		PDZ_signaling	1221..1308	CDD:238492	22/105 (21%)
		RhoGEF	1456..1647	CDD:238091	58/199 (29%)
		PH2_Tiam1_2	1650..1823	CDD:269957	30/199 (15%)
		PH	1693..1812	CDD:278594	22/130 (17%)
mcf2lb	XP_009303050.1	SEC14	50..193	CDD:214706	38/218 (17%)
		SPEC	328..>473	CDD:321951	32/177 (18%)
		RhoGEF	599..776	CDD:238091	57/198 (29%)
		PH-like	783..914	CDD:327399	27/175 (15%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	2	1.910