DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sif and tiam1a

DIOPT Version :9

Sequence 1:	NP_001261446.1	Gene:	sif / 43892	FlyBaseID:	FBgn0085447	Length:	2734	Species:	Drosophila melanogaster
Sequence 2:	XP_001924044.3	Gene:	tiam1a / 567621	ZFINID:	ZDB-GENE-080325-1	Length:	1621	Species:	Danio rerio

Alignment Length:	1757	Identity:	539/1757 - (30%)
Similarity:	797/1757 - (45%)	Gaps:	361/1757 - (20%)

- Green bases have known domain annotations that are detailed below.

  Fly   462 MGRASSQSSRFSGSRSSH-EIGRGYPPRNLYLELERERSCIEGSPPSDNVMFDNQCYATTPSSSN 525
            ||...||:...:.....| .|.|.:..|:|.:..::.|.....|  :..|...|. .::|.|||.
Zfish     1 MGNVESQNGDLAYYSQGHGHISRKHMTRSLRISNKQSRRSRHAS--TSKVEHRNS-ESSTKSSST 62

  Fly   526 GNSDQDQS-YGQQQSSGQHPQQQQGPP------QRSSRHQHHHQQAPNVTPTPGS---------- 573
            .:..|..| .|.:..|.::..:..|..      ..:.|....|....:.|.||||
Zfish    63 PSIPQSLSENGLEPFSEKNALESYGGTIWVDRMAMNLRPMSFHDNIGDPTITPGSEDGVEMDTVV 127

  Fly   574 ----PTSRLLLEYEMHLRNT-----------LAKGMDAESYSLHTFEALLSQ-SMENLEFAESLP 622
                ....|....:.:|:.|           ..:...|:.:...:.|..||. |.|:|...|.:.
Zfish   128 PDGCDDDGLFTGEDTYLQRTRDGPREGGSFKKKRSKSADMWRDDSMEFSLSDLSQEHLTSTEEII 192

  Fly   623 GSNQ---------------RSPYPL-----RRPNA--KSSTLPLPPHRPLSTIRDKERDRDRDGY 665
            |:|:               .||..|     .|.|:  :..|..|||.|....:        .:|:
Zfish   193 GTNEDEDKGFANCGGRRRTSSPRTLESHSGERANSLDELCTQRLPPLRGSHHL--------YNGH 249

  Fly   666 YSDRNELIRERE-----RERDRGY------------LSDHNSSFSNSRCASCIGESARAQWFRH- 712
            .::|.|.|...|     .|...||            ||:..|:...:.|:...|..|:.   .|
Zfish   250 NTERTEGIMNGEILSPAEEEGNGYGAYTLPCRRSHCLSEGLSNHQTTICSRMQGRRAQT---THD 311

  Fly   713 ---SDGWRSGSSTIGSGSGHGMMTQQIPGSGHK---RSPWDSLPSLRQDSSL--NDSGYKSARAD 769
               .:|...|.|.|..|:.      ::..|..:   .|...|:.|...:|..  :|||..|....
Zfish   312 ITAGEGSEYGDSGIDGGAA------EVEHSSRRCKAMSASFSVYSTASESIFAGSDSGSSSGGGG 370

  Fly   770 SLEQRAEFIRQDSLRSEYLSDRESRYGIVQQASIESTDSRMCYLTSSEISDDDRMSLTTAVSDED 834
            ..:|..:....::.|              |:..:.|..:|.|...:|..:.|:|.|.|
Zfish   371 DRQQGGDQGVYENFR--------------QELDMNSRQTRECLEEASSAASDERSSGT------- 414

  Fly   835 DGESVMASPYKAKATGTAASSFNCTGAVRKAGFLSVKKWLLRKKH-QIELARKRGWKGYWVCLKG 898
                 ::|.|.:....||..     |.|||||.|:||.:|:.||: ::|.|.||.||.|||.|||
Zfish   415 -----LSSIYPSDILLTAVQ-----GTVRKAGMLAVKNFLVHKKNKRVEPAAKRKWKQYWVSLKG 469

  Fly   899 TTLLFYPCDSREGRSVEAAPKHLIIVDGAIMQPIPEHPKRDYIFCLSTAFGDAYLFQAPCQVELE 963
            .||.||..|.|.|....:..||.:.|:.:|:|.:|||||:|::||||.|.|||:|||...|.|||
Zfish   470 CTLFFYETDGRSGIDHNSIHKHAVWVENSIVQAVPEHPKKDFVFCLSNALGDAFLFQTSGQTELE 534

  Fly   964 NWVNSIHSACAAAFARHRGKTGTLHLLQEEIFRLEKAIESDHKLKHMAELQQSVVTDQETRHQIQ 1028
            ||:.:||||||||.||...:..|:.||:.||.:||:.|:.|.|:|.|.|:|.|.|||.:.|..|.
Zfish   535 NWITAIHSACAAAVARQHHREDTVRLLRSEIKKLEQKIDMDEKMKKMGEMQLSAVTDGKKRKTIL 599

  Fly  1029 TQILQWEENLERLHCEQFRLRCYMASLQSGELPNPKSLLTHVSRPTKNTLNKLGVFTVSSFHAFI 1093
            .||..||:|||:.|.:.||.|||::|||.|||||||.||...|||||.::.:||:|:||||||.:
Zfish   600 DQIFLWEQNLEQFHMDLFRYRCYLSSLQGGELPNPKRLLAVASRPTKLSMGRLGIFSVSSFHALV 664

  Fly  1094 CARSPSLLNNLLAGRGATKRRPPMLSRSNSGSSRRSMQMNS------------------------ 1134
            .||:.          |..:||...||||   ||:|..:.:|
Zfish   665 AARTD----------GGIRRRTQALSRS---SSKRKSRFSSLWGLDTTSKKKTKGRPSINQVFVD 716

  Fly  1135 RDEP---------EKTFK---------------------------------VAMPDNAYSTVYLR 1157
            .::|         |.|.|                                 |::||:......::
Zfish   717 GEQPVKRNLEHMFEDTGKNTLHKDKDGSLKSLPQHKTDNEVWVPDYLTPSWVSLPDDRPVLAIIQ 781

  Fly  1158 DAMSVEEFLASACARRNLNPMEHFVRVKKRRDMEDHNYFVPHRNDLIENYLHNHEFVEVCMKILY 1222
            ...:..|.|.:.|....|...:|::|:|...|.:...| ||...:.:.:.|:..  :|:|.|...
Zfish   782 PGETAMEALEALCKVHKLELSKHYIRLKFLIDNQVQLY-VPRPEEDLCDLLYKE--IEICAKTTK 843

  Fly  1223 QVELQR-TTLEQMWGFSVEAELIENAERQDELCCYVSRVEDKSVAMHNGIIKGDEIMVINGAIVS 1286
            .::..| .:....:|||| |.:.|:..:|    .:::.|:...:|...|:..||||:.:|....|
Zfish   844 VIQFDRDESCAIGYGFSV-AVVEEDGGQQ----LHITDVKAGGLAFAKGLRAGDEILKLNSKPAS 903

  Fly  1287 DLDMMYLESVLQEEQSLSMMMRSSRTEPPDLVGIMRVTDDMIDSLVCPPPP----------TDPP 1341
            .|::..:::.. ...||::.:.:.....|              ..:|..||          ||..
Zfish   904 VLELADMQAAF-ALPSLTLTVSTLPAADP--------------HQLCQLPPRRSDGAQDLCTDIF 953

  Fly  1342 VMSEEMI----TGLIVPAPGWNGTSKDLYSPEAESSPATSFVDPAAMAAQLAVGGVGGVVVGGLG 1402
            ..|:|.|    .||::.:|  :.|..|..:..|| |||.|..|....|
Zfish   954 SQSQEDILDEGLGLVLHSP--DDTGDDRVNLMAE-SPADSLEDEIDSA----------------- 998

  Fly  1403 VAKPTSRTSSFEIENLLKTAEQETRKSSPTGSVTSSVSTTAL-----------TPSRQLTDAEKL 1456
             .|.|.:.::| ..||......|...|..:.|.|||.|::.:           || |||:||:||
Zfish   999 -HKSTEQVTAF-CRNLHDMNSNEGPVSCSSSSSTSSSSSSCMPSPISPLPGLFTP-RQLSDADKL 1060

  Fly  1457 RKVVMELVDTERTYVKHLNNLLEHYLEPMKRETFLSNAEINALFGNIHEIVTFQRQFLQNLEESL 1521
            |||:.|||||||||||.||.|:|.||.|:::|:||:..|::.||||:.|:|.||.:||:.||:..
Zfish  1061 RKVISELVDTERTYVKDLNILIERYLNPLQKESFLTQDELDVLFGNLAEMVEFQVEFLKTLEDGT 1125

  Fly  1522 DLEPDFNKFEHCGQFRNVLFAIGSAFLYYVNHFKLYSSFCASHSKAQKVLHPNEGNHALQEFLAA 1586
            .|.||.:|.|...||:.|||::|.:||||.:.||:||:|||||:|..|||...:.:...:.|||.
Zfish  1126 RLVPDLDKLERVDQFKKVLFSLGGSFLYYADRFKIYSAFCASHTKVPKVLTKAKTDPEFKAFLAE 1190

  Fly  1587 RNPKQQHSSTLESYLIKPIQRILKYPLLLQQMRNLTDTRADEHVHLCEALKGMEKVAEHINEMQR 1651
            |||:|||||||||||||||||:|||||||:::.:|||..::||.||..|:|.|.|||.||||||:
Zfish  1191 RNPRQQHSSTLESYLIKPIQRVLKYPLLLRELYSLTDPDSEEHYHLDVAMKAMNKVASHINEMQK 1255

  Fly  1652 IHEEYGAIFDHLFRQHQKSCKQPIDLSPGDLLYYGGVEWLNISDFLGKIKKGLELHAMCFVFKSA 1716
            |||||||:||.|..:.....|:..|||.||||.|..|.|:|....|.|.|:..||.|  |||::|
Zfish  1256 IHEEYGAVFDQLISEQSSEKKEVADLSMGDLLLYDTVVWINPPSSLMKGKRDPELAA--FVFRTA 1318

  Fly  1717 VVFLCKERLRQKKKLMGVSSKNATNEVEIIRYQVLIPVTEVQVRASSAKDMDSHFLWELIHLRSQ 1781
            |||:||:..:||||:.|....:..:|.:..|::.:|....:|||  :..:.:...:.|::|:||:
Zfish  1319 VVFVCKDCSKQKKKIGGPHRVSTLDERDPFRFRHMISTDALQVR--NLPNSEGSAMCEIVHVRSE 1381

  Fly  1782 LQRRSEKVYVLSNSTADFRNAFLKTIRQIIRESVRNMSIPMKNFGGSSGSVSGHSSQGMGSMGYP 1846
            .:.|.|:.:.:..|:.:.:..||||:..|:|:..|.                    |.|.:...|
Zfish  1382 FEGRPERTFHICCSSPESKKDFLKTVHSILRDKQRR--------------------QLMKTESLP 1426

  Fly  1847 GNSQTL------------ERPKQQITIVHGSHTLGKPKKKSGSQRHSAGNIDYDNLSGSQEADDL 1899
            .|.|.:            .||.....:...|.|||:.|..........|.|..|:  .||:...:
Zfish  1427 PNQQYIPFGGKRLCALKGSRPTMNRAVSAPSRTLGQRKLIRNRITIDTGVIFDDD--SSQDLSPI 1489

  Fly  1900 PPSVGVVHYASGHTHGQQMQPAGFRGRSKTVGDVTEITCSSPEPHQQQQQQQQQQQQLMQQGHAH 1964
            .|:....|        :..||  .|...:.|.|..::..       .:.|...::..::.....:
Zfish  1490 TPTEPPFH--------KPQQP--IRDTDRWVEDQFDLQI-------YENQDDVKETDILSDDDEY 1537

  Fly  1965 AHPHPHPHPREPPPPPIRQPHLHHHSSD--IERIDPGTKSEGEED-SQQGTIRPKATLGRTPNHL 2026
            ..             .:|.|     |||  :|........||:|| ...|....|:.....|..|
Zfish  1538 CQ-------------SVRAP-----SSDPSLEESFEALSVEGDEDKGLNGLSEDKSKKSHAPLKL 1584

  Fly  2027 TL 2028
            ||
Zfish  1585 TL 1586

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937	69/125 (55%)
		PH	873..974	CDD:278594	54/101 (53%)
		TIAM1_RBD	1141..1219	CDD:176413	19/110 (17%)
		PDZ_signaling	1221..1308	CDD:238492	20/87 (23%)
		RhoGEF	1456..1647	CDD:238091	110/190 (58%)
		PH2_Tiam1_2	1650..1823	CDD:269957	63/172 (37%)
		PH	1693..1812	CDD:278594	38/118 (32%)
tiam1a	XP_001924044.3	PH1_Tiam1_2	430..556	CDD:269937	69/125 (55%)
		PH	443..545	CDD:278594	54/101 (53%)
		UBQ	765..840	CDD:294102	17/77 (22%)
		PDZ_signaling	842..923	CDD:238492	20/86 (23%)
		RhoGEF	1063..1252	CDD:214619	108/188 (57%)
		PH2_Tiam1_2	1254..1423	CDD:269957	65/192 (34%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	233	1.000	Domainoid score	I2328
eggNOG	1	0.900	-	-		E1_KOG3519
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0002962
OrthoInspector	1	1.000	-	-		otm26125
orthoMCL	1	0.900	-	-		OOG6_105244
Panther	1	1.100	-	-	O	PTHR46001
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3224
SonicParanoid	1	1.000	-	-		X2782
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	12	11.800