DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sif and itsn2a

DIOPT Version :9

Sequence 1:	NP_001261446.1	Gene:	sif / 43892	FlyBaseID:	FBgn0085447	Length:	2734	Species:	Drosophila melanogaster
Sequence 2:	XP_021324162.1	Gene:	itsn2a / 566412	ZFINID:	ZDB-GENE-041014-84	Length:	1666	Species:	Danio rerio

Alignment Length:	1608	Identity:	305/1608 - (18%)
Similarity:	533/1608 - (33%)	Gaps:	447/1608 - (27%)

- Green bases have known domain annotations that are detailed below.

  Fly   402 PMPSTESPVTSPEMGRRRYNYYNANAAQTLGHPPHMHQHGMAMGMGGGGGGGHHIMN-NNTMGRA 465
            |:|...:.::.|.:.       |.||......|        |..:..|....|.::: .::...:
Zfish   148 PLPQMPNSISVPALP-------NGNATLLTPTP--------AFPVSSGLTKSHSLLDLGSSSSNS 197

  Fly   466 SSQSSRFSGSRSSHEIGRGYPP------RNLYLELERERSCIEGSPPSDNVMFDNQCYATTPSSS 524
            ||.:|..|.|..:.......|.      |.|:..|::..|.....|...|.:..:....|..::.
Zfish   198 SSTTSLASNSPKTSSSDWAVPQSSRLKYRQLFNSLDKLMSGYLSGPQVRNALTASNLTQTQLATI 262

  Fly   525 NGNSDQDQSYGQQQSSGQHPQQQQGPPQRSSRHQHHHQQAPNVTPTPGSPTSRLLLEYEM---HL 586
            ...:|.|:. ||.::            :......|..:.|....|.|      |.|..::   .|
Zfish   263 WFLADVDRD-GQLRA------------EEFILAMHLVEMAKTGRPLP------LTLPPDLVPPSL 308

  Fly   587 RNTLAKGMD-----AESYSLHTFEALLSQ-SMENLEFAESLPGSNQRSPYPLRRPNAKSSTLPLP 645
            |.|  |..|     ....|....|..|.| :..|:.:.:.|..:.||....|.:   :...|...
Zfish   309 RTT--KSCDLLNGPVPLISTELIEPELPQKNKSNVSYEDKLRENFQRGNAELEK---RRLALQEQ 368

  Fly   646 PHRPLSTIRDKERDRDRDGYYSDRNEL-IRERE-------RERDRGYLSDHNSSFSNSRCASCIG 702
            ..|.....|::||..:......:|.|. .||||       |:|:.....:........|....:.
Zfish   369 QRREEERRREEERREEEKRQQKEREERERREREAREMEIRRQREEERRMERQRELERQREEERLK 433

  Fly   703 ESARAQWFRHSDGWRSGSSTIGSGSGHGMMTQQIPGSGHKRSPWDSLPSL----RQDSSLNDSGY 763
            |..|.:                            .....:|..|:....:    :::...||...
Zfish   434 ELERRE----------------------------EAERQRRREWEKSKRVELQTQKEKEQNDIQR 470

  Fly   764 KSARADSLEQRAEFI--RQDSLRSEYLSDRESRYGIVQQASIESTDSR--MCYLTSSEISDDDRM 824
            ...|..|||...|.:  :...:.|:....:..|.|  |.|.:|..:.|  :|      |:|.:.:
Zfish   471 LKDRKRSLEIELEAVGNKHKQISSQLRDAKSKRQG--QWAELELINQRRDVC------ITDINSL 527

  Fly   825 SLTTAVSDEDDGESVMASPYKAKATGTAASSFNCTGAVRKAGFLSVKKWLLRK-------KHQIE 882
            .|.   .||...:.....|.|.:   .|....:.|..:.......||:.:..|       |.|::
Zfish   528 QLQ---FDEMQRQLNQLGPEKQR---LADRLVHFTQNISSPLINDVKQSVCEKDRSCRKLKEQLD 586

  Fly   883 LARKRGWKGYWVCLKGTTLLFYPCD--SREGRSVEAAPKHLIIVDGAIMQPIPEHPKRDYIFCLS 945
            :..:.           ||......:  ::|.:.||        :|..::|.:     ...:.||:
Zfish   587 VLERE-----------TTAKLSQMEQYNKEIKFVE--------MDDCVLQGL-----LSLLGCLT 627

  Fly   946 TAF---GDAYLFQAPCQVELENWVNSIHSACAAAFARHRGKTGTLHLLQEEIFRLEKAIESDHKL 1007
            ..|   .:....|:..|..||. :..:.|.......|||        .:||    |:..:.:.:.
Zfish   628 NLFLLIKELRQKQSQQQDVLEQ-LRRVRSDKLKELQRHR--------KEEE----ERKRKKEEEA 679

  Fly  1008 KHMAELQQSVVTDQETRHQIQTQILQWEENLERLHCEQFRLRCYMASLQSGELPNPKSLLTHVSR 1072
            ...|:|:|..:..:|...|.:..    :|...:|..||.| :.....|::.|....|.   ...|
Zfish   680 ARQAQLEQERLEQEEAERQRKLA----QEREAKLREEQQR-QALARLLEAQEQARAKE---EKRR 736

  Fly  1073 PTKNTLNKLGVFTVSS--FHAFICARSPSLLNNLLAGRGATKRRP---PMLSRSNSGSSRRSMQM 1132
            ..:...|:|.|.|.||  |:        |..||:|        :|   |.::.|:..:.|.....
Zfish   737 AEEKERNELDVHTQSSLPFN--------STENNIL--------QPVVIPEVTCSSLTTYRALYPF 785

  Fly  1133 NSRDEPEKTFKVAMPDNAYSTVYLRDAMSVEE---FLASACARRNLNPMEHFVRVKK-------- 1186
            .:|:..|.|.:.    :.:..|   |..:|.|   ...|....|...|..:..|..|
Zfish   786 TARNSDELTLEA----DCFIQV---DESTVRETGWLYGSYSGNRGWFPESYAERCSKDSQTQPTA 843

  Fly  1187 ----------------RRDMEDHNYFVPHRNDLIENYLHNHEFVEVCMKILYQVELQRTTLEQMW 1235
                            |.|:|..   .|....:..|..|: :.|.:|       |....| :...
Zfish   844 AETESTAPSANYPGIPRVDIEGP---TPTHTPVSTNTQHS-QAVALC-------EWSAKT-DSHL 896

  Fly  1236 GFSVEAELIENAERQD-----EL--------CCYVSRV---EDKSVAMHNGIIKGDEIMVINGAI 1284
            ||..: ::|...|:|:     ||        |.:||.|   ..:|..:::.:   :||.|.:..:
Zfish   897 GFCKD-DIITVLEKQENWWYGELKEKRGWFPCSHVSVVTTNNTQSEPLYSSV---EEIEVSDSGL 957

  Fly  1285 VSDLDMMY-LESVLQEEQSLSMMMRSSRTEPPDLVGIMRVTDDMIDSLVCPPPPTDPPVMSEEMI 1348
            :.:...:| .||                                       |...|....:|:::
Zfish   958 LDEYVALYTYES---------------------------------------PESGDLTFSAEDVV 983

  Fly  1349 TGLIVPAPG--WNGTSKD---LY--------SPEAESSPATS---------FVDPAAMAAQLAVG 1391
              |:....|  |.|...|   |:        .|:..||...|         .|.||....||::.
Zfish   984 --LVTEKEGEWWRGCIGDQTGLFPSNYVKPKEPDTASSAVPSKKPEIAQVTTVSPATTPEQLSLT 1046

  Fly  1392 GVGGVVV----------GGLGVAKPTSRTSSFEIENLLKTAEQETRKSSPT-------------- 1432
            ....:||          |.|.......:...|:..| ::..|..:.|.:|.
Zfish  1047 PGQLIVVLHKNSNSWWLGELQARGKKRKKGWFQSSN-VRLLEANSGKITPAPQPLCQVIAMYDYK 1110

  Fly  1433 ---------------------------GSV--------TSSVS-TTALTPSRQ-----------L 1450
                                       |.|        |:.|. ||...||:|           .
Zfish  1111 AANKDEMSFQKGQLITVLNKDNPDWWKGEVAGVTGLFPTNYVKMTTECDPSQQWCADLLSLDSMC 1175

  Fly  1451 TDAEKLRKVVMELVDTERTYVKHLNNLLEHYLEPMKRETFLSNAEINALFGNIHEIVTFQRQFLQ 1515
            |:..|.:..:.||:.||.:|::.|...:|.:.:||.....|:.|||:.:|.|..|::....:.|:
Zfish  1176 TEERKRQGYIHELIQTEESYLEDLELAVEVFYKPMAESGRLTEAEISMIFVNWRELIMCSTKLLK 1240

  Fly  1516 NLEESLDLEPDFNKFEHCGQFRNVLFAIGSAFLYYVNHFKLYSSFCASHSKAQKVLHPNEGNHAL 1580
            .|..         :.:..|: |..:..:|......::|.:.|..||:....|..:|. .:.:.:|
Zfish  1241 ALRV---------RKKTAGE-RMPVQVVGDILSSELSHMQAYIRFCSCQLNAAALLQ-QKTDKSL 1294

  Fly  1581 QEFLAARNPKQQH---SSTLESYLIKPIQRILKYPLLLQQMRNLTDTRADEHVHLCEALKGMEKV 1642
            ...|..:.....:   ...|.|:|:||:|||.:||||::.:...|.....:|.:|..||:..|::
Zfish  1295 DFKLFLKKIASNYRCKGMPLSSFLLKPMQRITRYPLLIKNILENTPPAHADHSNLQAALEQAEEL 1359

  Fly  1643 AEHINEMQRIHEE------------YGAIFDHLFRQHQKSCKQPIDLSPGDLLYYGGVEWLNISD 1695
            ...:||..|..|.            ...:.:||......:|     |.|..||:.|.
Zfish  1360 CSQVNEAVREKENSDRLEWIQNHVLCDGVIEHLVFNSLTNC-----LGPRKLLHSGK-------- 1411

  Fly  1696 FLGKIKKGLELHAMCFVFKSAVVFLCKERLRQKKKLMGVSSKNATNEVEIIRYQVLIPVTEVQVR 1760
             |.|.|...||.|:.|.....:.:..|:......||.     |..:..:...|:..:.:.||.|:
Zfish  1412 -LHKTKSSKELWALLFNDFLLLTYTSKQFSSSPDKLF-----NPNSNTQYKMYKTPVFLNEVLVK 1470

  Fly  1761 ASSAKDMDSHFLWELIHLRSQLQRRSEKVYVLSNSTADFRNAFLKTIR 1808
            ..|....|.. ::.:.|:        ::||.|...|.:.|..:::.|:
Zfish  1471 MPSDPSSDDP-VFHISHI--------DRVYTLKTDTINERTTWVQKIK 1509

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937	23/136 (17%)
		PH	873..974	CDD:278594	18/112 (16%)
		TIAM1_RBD	1141..1219	CDD:176413	18/104 (17%)
		PDZ_signaling	1221..1308	CDD:238492	20/103 (19%)
		RhoGEF	1456..1647	CDD:238091	47/193 (24%)
		PH2_Tiam1_2	1650..1823	CDD:269957	34/171 (20%)
		PH	1693..1812	CDD:278594	24/116 (21%)
itsn2a	XP_021324162.1	EH	7..92	CDD:197477
		EH	214..293	CDD:197477	14/91 (15%)
		SMC_N	453..>727	CDD:330553	61/329 (19%)
		SH3_Intersectin2_1	776..832	CDD:212921	11/62 (18%)
		SH3	881..932	CDD:327375	13/59 (22%)
		SH3_Intersectin_3	960..1011	CDD:212772	12/91 (13%)
		SH3_Intersectin2_4	1028..1086	CDD:212927	11/58 (19%)
		SH3	1101..1154	CDD:327375	4/52 (8%)
		RhoGEF	1181..1364	CDD:238091	47/193 (24%)
		PH_13	1383..1523	CDD:318790	32/155 (21%)
		C2_Intersectin	1524..1658	CDD:176021
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	2	1.910