DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sif and arhgef12a

DIOPT Version :9

Sequence 1:	NP_001261446.1	Gene:	sif / 43892	FlyBaseID:	FBgn0085447	Length:	2734	Species:	Drosophila melanogaster
Sequence 2:	XP_009290006.1	Gene:	arhgef12a / 562455	ZFINID:	ZDB-GENE-121017-3	Length:	1357	Species:	Danio rerio

Alignment Length:	1824	Identity:	341/1824 - (18%)
Similarity:	554/1824 - (30%)	Gaps:	704/1824 - (38%)

- Green bases have known domain annotations that are detailed below.

  Fly   459 NNTMGRASSQSSRFSGSRSSHEIGRGYPPRNLYLELERERS-CIEGSP-----PSDNVMFDNQCY 517
            ::|....|.::|.|        :.|.:||.      :|.:| .:.|:|     |:|.::..:.|.
Zfish     2 SDTRSTVSDRTSSF--------LSRDHPPD------KRPKSDKLPGTPTHEFDPTDVLVQKSGCV 52

  Fly   518 ATTPSSSNGNSDQDQSYGQQQS---------------SGQHP-----QQQQGPPQRSSRHQHHHQ 562
            ..        ..:.:|.|..|.               ||.:|     .:|.|...|:
Zfish    53 VA--------DRRPESCGLVQRCVIIQKDENGFGLTVSGDNPVFVQLVKQDGAAMRA-------- 101

  Fly   563 QAPNVTPTPGSPTSRLLLEYEMHLRNTLAKGMDAESYSLHTFEALLSQSMENLEFAESLPGSNQR 627
                     |..|...:::    :..||.    .:|..:...:.:.|.|...|......||..|
Zfish   102 ---------GVQTGDRIIK----VNGTLV----TQSNHVEVVKLIKSGSYVALTVLGRPPGLPQ- 148

  Fly   628 SPYP----------------LRRPN----AKSSTLPLPPHRPLSTIRDKERDRDRDGYYSDRNEL 672
            .|.|                :..||    |:.::.||......|.:.::..|..|.|
Zfish   149 IPLPEVDVGLESPDVFGDPFITSPNSYNAAERTSSPLVSWEENSALHNQRMDSRRTG-------- 205

  Fly   673 IRERERERDRGYLSDHNSSFSNSRCASCIGESARAQWFRHSDGWRSGSSTIGSGSGHGMMTQQIP 737
                        |.|.    .:::.:.|:..|....| ...|.::...|.....||:     .:|
Zfish   206 ------------LKDQ----QDTQESPCLRNSEDGSW-AEEDQYKRDFSPEAFWSGN-----SVP 248

  Fly   738 GSGHKRSPWDSLPSLRQDSSLNDSGYKSARADSLEQRAEFIRQDSLRSEYLSDRESRYGIVQQAS 802
             |.|:|. :.:.|..::||  |.|..||...||      |....|...:|.||.:||:
Zfish   249 -SVHRRF-YPASPESQRDS--NCSSPKSTPRDS------FNSCPSPEVDYGSDLDSRF------- 296

  Fly   803 IESTDSRMCYLTSSEISDDDRMSLTTAVSDED-DGESVMASPYKAKATGTAASSFNCTGAVRKAG 866
                        .|.:.....:|...|..|:| |.|....:           ...:|
Zfish   297 ------------PSTVGSPRLVSEIIAAEDDDFDSEQEQVN-----------GQCSC-------- 330

  Fly   867 FLSVKKWLLRKKH----------QIELARKRGWKGYWVCLKGTTLLFY-------PCDSREGRSV 914
            |.|::....|..|          |.:.|               .||.|       ..:|:|.|.:
Zfish   331 FQSIQLLKTRPAHLAAFLHHVISQFDPA---------------PLLCYLYADLYKQTNSKETRRI 380

  Fly   915 EAAPKHLIIVDGAIM-----------------QPIPEHPKRDYIFCL-STAFGDAYLFQAPCQVE 961
            ........|..||.:                 :.|||...|.||..: .|.|.|.       |..
Zfish   381 FTDFLTFFIDRGANLKVTVPESISSELERRRPELIPEELHRQYIQTMQETLFPDV-------QRN 438

  Fly   962 LENWVNSIHSACAAAFARHRGKTGTLHLLQEEIFRLEK---AIESDHKLKH--MAELQQSVVTDQ 1021
            ||::             |.:...| |.|...|:.||:.   |::.:.....  ::::...::|.|
Zfish   439 LEDF-------------RQKRSMG-LTLADTELSRLDSEQLAVDRERACAESILSKIDDVLLTSQ 489

  Fly  1022 ETRHQ----IQTQILQWEENL-----ERLHCEQFRLRCYMASLQSGELPNPKSLLTHVSRPTKNT 1077
            .|..:    ||..|..:.::|     |:.:.|..|:|...       ||:.    ...|:..|:.
Zfish   490 PTEEEKCSTIQYVIFMYMKHLGVRAKEQRNLESKRVRINF-------LPSK----LRKSKTEKDG 543

  Fly  1078 LNKLGVFTVSSFHAFICARSPSLLNNLLAGR--GATKRRP------PMLSRS---NSGSSRRSMQ 1131
            ..||   ..|.|...:..|.|..|:::.:||  .:.|:||      |.|..|   ::|..|.|..
Zfish   544 EEKL---KKSRFPIRVPPRWPGRLDSVPSGRVGDSIKQRPQKQMSQPALGGSELIDAGRLRGSQS 605

  Fly  1132 MNSRD---EPEKTFKVAMPDNAYSTVYL-------RDAMSVEEFLASACARRNLNPMEHFVRVKK 1186
            ....|   .|.     .:..:.:|..|.       ||:    ||        |::|
Zfish   606 SEGSDLLHSPG-----LITSSPFSAPYSSPSDPSGRDS----EF--------NMSP--------- 644

  Fly  1187 RRDMEDHNYFVPHRNDLIENYLHNHEFVEVCMKILYQVELQRTTLEQMWGFSVEAELIENAERQD 1251
                   :..||..:|.:::..........|..|.|.|                          |
Zfish   645 -------SAVVPRLSDGLQSGDPLDSIYPPCDYIPYNV--------------------------D 676

  Fly  1252 ELCCYVSRVEDKSVAMHNGIIKGDEIMVINGAIVSDLDMMYLESVLQEEQSLSMMMRSSRTEPPD 1316
            :|      .||:.         ||                                     .|||
Zfish   677 QL------DEDRD---------GD-------------------------------------RPPD 689

  Fly  1317 LVGIMRVTDDMIDSLVCPPPPTDPPVMSEEMITGLIVPAPGWNGTSKDLYSPEAESSPATSFVDP 1381
             ||                                 .|.|  :...:||.|.:.:.|...|.|||
Zfish   690 -VG---------------------------------TPKP--SRRFEDLQSEDDQGSDVYSDVDP 718

  Fly  1382 AAMAAQLAVGGVGGVVVGGLGVAKPTSRTSSFEIENLLKTAEQETRKSSPTGSVTSSVSTTALTP 1446
            ... .||    ||..|:.|                                           |||
Zfish   719 PNW-QQL----VGRDVLAG-------------------------------------------LTP 735

  Fly  1447 SRQLTDAEKLRKVVMELVDTERTYVKHLNNLLEHYLEPMKRETFLSNAEINALFGNIHEIVTFQR 1511
            ..     .|.::|:.||..|||.:|:.|..|.:.:.:.:.|:..|..|:|..:|.|:.||:....
Zfish   736 HE-----IKRQEVINELFYTERAHVRMLKVLDKVFYQKLNRDNILPPADIKYIFTNLEEIIQLHV 795

  Fly  1512 QFLQNLEESLDLEPDFNKFEH----------CGQFRNVLFAIGSAFLYYVNHFKLYSSFCASHSK 1566
            :.:..: .::..:.:.:..:|          .|:...:..|:|              :||::...
Zfish   796 RIVDQM-TAICKKNETSVIDHIGDELLSWFSSGEEEKIKQAVG--------------TFCSNQPF 845

  Fly  1567 AQKVLHPNEGNHALQEFLAARNPKQQHSST---------------LESYLIKPIQRILKYPLLLQ 1616
            |             .|.:.:|..|.|..|:               |:..:...:||..|||||:.
Zfish   846 A-------------LELIKSRQKKDQRFSSFMQEAESNRLCRRLQLKDIIPAEMQRFTKYPLLMD 897

  Fly  1617 QMRNLTDTRADEHVHLCEALKGMEKVAEHINEMQRIHEEYGAIFDHLFRQHQKSCKQP------- 1674
            .:...|| .::|...:..|.:...::..|:|:..:..|....:.|:..|....|.||.
Zfish   898 NIAKYTD-ESEEKDKVRRAAECCRQILNHVNQSVKESENKQRLEDYQRRLDLSSLKQSENPMISE 961

  Fly  1675 ---IDLSPGDLLYYGGVEWLNISDFLGKIKKGLELHAMCFVFKSAVVFLCKERLRQKKKLMGVSS 1736
               :||:...:::.|.:.|        ||.|...:.....:.:..:|.|.|:   .::.::...|
Zfish   962 FKNLDLTKRKMVHEGPLSW--------KINKDKTIELYTLLLEDILVLLQKQ---DERLILRCHS 1015

  Fly  1737 KN--ATNEVEIIRYQVLIPVTEVQVRASSAKDMDSHFLWELIHLRSQLQRRSEKVYVLSNSTADF 1799
            ||  .|.|.:.| :..:|.::.|.|| |.|.|..|.|:       ..:.:...::|.|...|...
Zfish  1016 KNLAGTAETKHI-FSPIIKLSTVLVR-SVATDNRSFFV-------ISMSKNGAQIYELMAQTVSE 1071

  Fly  1800 RNAFLKTIRQIIRESVRNMS----IPMKNFGGSSGSVSGHSSQGMGSMGYPGNSQTLERPKQQIT 1860
            :..:.:.|.|  |..|.|..    ||:....|....|.
Zfish  1072 QRMWQRQITQ--RAEVMNTKPHSVIPLPQHDGDRDGVE--------------------------- 1107

  Fly  1861 IVHGSHTLGKPKKKSGSQRHSAGNIDYDNLSGSQEAD-DLPPSVGVVHYASGHTHGQQMQPAGFR 1924
                   |.|..|  ||.|.|.|:|.      |.|.| :..||..:.........|::.|.|. .
Zfish  1108 -------LSKFSK--GSDRISTGSIQ------SAEKDGEFSPSAALRDCVPDTNPGEEDQVAR-A 1156

  Fly  1925 GRSKTVGD--------VTEITCSSPEPHQQQQQQQQQQ---QQLMQQGHAHAHPHPHPHPREPPP 1978
            ||..:..|        ...::|||......:.....:|   .|||.|
Zfish  1157 GRDASYQDSELLNGRETDSLSCSSKADEALKTLAALKQVLLTQLMAQ------------------ 1203

  Fly  1979 PPIRQPHLHHHSSDIERIDPGTKSEGEE-DSQQGTIRPKATLGRTP----NHLTLSTTSTLSVGS 2038
                                   .|||. ....|:...:.|..|||    ::.|.|.....:.|.
Zfish  1204 -----------------------DEGERLGRSAGSRLLRTTSLRTPVEHNSNRTPSRAQDYTSGD 1245

  Fly  2039 TGSQARLIQSSHPPASYQPVLMKDLGKPSPDESELESESEV--------H--AAQVAASFNLSLG 2093
            ||..       ..|..|...|:.: |.....||..:.:.:|        |  ||....|...|..
Zfish  1246 TGFY-------ECPEDYAAYLVLE-GFGGSGESSTDDDFQVGRKEPGLAHSCAADSGISLKFSSP 1302

  Fly  2094 SSGS 2097
            ||||
Zfish  1303 SSGS 1306

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937	28/159 (18%)
		PH	873..974	CDD:278594	25/135 (19%)
		TIAM1_RBD	1141..1219	CDD:176413	12/84 (14%)
		PDZ_signaling	1221..1308	CDD:238492	8/86 (9%)
		RhoGEF	1456..1647	CDD:238091	42/215 (20%)
		PH2_Tiam1_2	1650..1823	CDD:269957	41/188 (22%)
		PH	1693..1812	CDD:278594	26/120 (22%)
arhgef12a	XP_009290006.1	PDZ_signaling	66..140	CDD:238492	14/98 (14%)
		RGS	308..524	CDD:295367	50/270 (19%)
		RhoGEF	743..928	CDD:279015	42/213 (20%)
		PH-like	947..1083	CDD:302622	33/157 (21%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	1	0.910