DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sif and FGD6

DIOPT Version :9

Sequence 1:	NP_001261446.1	Gene:	sif / 43892	FlyBaseID:	FBgn0085447	Length:	2734	Species:	Drosophila melanogaster
Sequence 2:	NP_060821.3	Gene:	FGD6 / 55785	HGNCID:	21740	Length:	1430	Species:	Homo sapiens

Alignment Length:	1547	Identity:	282/1547 - (18%)
Similarity:	498/1547 - (32%)	Gaps:	455/1547 - (29%)

- Green bases have known domain annotations that are detailed below.

  Fly   554 SSRHQHHHQQAPNVTPTP------------GSPTSRLLLEYEMHLRNTLAKGMDAESYSLHTFEA 606
            ||..|...:..|.:.|.|            .||:.:::|..|.| :..||:..|       .|..
Human    39 SSVPQSTKKMKPAIAPKPKVLKTSPVREIGQSPSRKIMLNLEGH-KQELAESTD-------NFNC 95

  Fly   607 LLSQSMENLEFAESLPGSNQRSPYPL-RRPNAKSSTLPLPPHRPLSTIRDKERDRDRDGYYSDRN 670
            ....:..| ::...:...:....:.| .|.|.....|.|.|         .|.:.:.:....|..
Human    96 KYEGNQSN-DYISPMCSCSSECIHKLGHRENLCVKQLVLEP---------LEMNENLENSKIDET 150

  Fly   671 ELIRERERERDRGYLSDHNSSFSNSRCASCIGESARAQWFRHSDGWRSGSSTIGSGSGHGMMTQQ 735
            ..|:.|                  |:| ...||.|:.|     .|....:|.:.......::.|.
Human   151 LTIKTR------------------SKC-DLYGEKAKNQ-----GGVVLKASVLEEELKDALIHQM 191

  Fly   736 IPG-SGHKRSPWDSLPSLRQDSSLNDSGYKSARADSLEQRAEFIRQDSLRSEYLSDRESRYGIVQ 799
            .|. |..|..|.|| |.:  :...|.:|         :.|.||..        ||...|.:..|
Human   192 PPFISAQKHRPTDS-PEM--NGGCNSNG---------QFRIEFAD--------LSPSPSSFEKV- 235

  Fly   800 QASIESTDSRMCYLTSSEISDDDRMSLTTAVSDEDDGESVMASPYKAKATGTAASSFNCTGAVRK 864
                  .|...|:|   ::..|:.....|.   :||.|                .|.||.
Human   236 ------PDHHSCHL---QLPSDECEHFETC---QDDSE----------------KSNNCF----- 267

  Fly   865 AGFLSVKKWLLRKKHQIELARKRGWKGYWVCLKGTTLLFYPCDSREGRSVEAAPK-------HLI 922
                        :..::| |.:.|        |.:||:     |.:|.|.::..|       ||:
Human   268 ------------QSSELE-ALENG--------KRSTLI-----SSDGVSKKSEVKDLGPLEIHLV 306

  Fly   923 IVDGAIMQPIPEHPKRDYIF---CLSTAFGDAYLFQAPCQVELENWVNSIHSACAAAFARHRGKT 984
            ........|.|...:...:.   |:.|          |.:...|...:...|:|....:....|.
Human   307 PYTPKFPTPKPRKTRTARLLRQKCVDT----------PSESTEEPGNSDSSSSCLTENSLKINKI 361

  Fly   985 GTLH----LLQEEIFRLEKAIESDHKLKHMAELQ-----QSVVTDQETRHQIQTQILQWEENLER 1040
            ..||    ..||::.:::...:|:..::..::.|     |..:.::.|..:........:.||..
Human   362 SVLHQNVLCKQEQVDKMKLGNKSELNMESNSDAQDLVNSQKAMCNETTSFEKMAPSFDKDSNLSS 426

  Fly  1041 --------------------LHC-------EQFRLRCYMASLQSGE--------------LPNPK 1064
                                :.|       :|.:|.| ...||||.              :....
Human   427 DSTTVDGSSMSLAVDEGTGFIRCTVSMSLPKQLKLTC-NEHLQSGRNLGVSAPQMQKESVIKEEN 490

  Fly  1065 SLLTHVSRPTKNTLNKLGVFTVSSFHAFICARSPSLLNNLLAGRGATKRRPPMLSRS-------- 1121
            ||.....:|.:::|...||...        |.|..||..........|.....|.|:
Human   491 SLRIVPKKPQRHSLPATGVLKK--------AASEELLEKSSYPSSEEKSSEKSLERNHLQHLCAQ 547

  Fly  1122 NSGSSRRSMQMNSRDEPEKTFKVAMPDNAYSTVYLRDAMSVEEFLASACARRNLNPMEHFV--RV 1184
            |.|.| .|..|..|...:..:|:..|...:|        ...|||.|.....|..|.....  |.
Human   548 NRGVS-SSFDMPKRASEKPVWKLPHPILPFS--------GNPEFLKSVTVSSNSEPSTALTKPRA 603

  Fly  1185 KKRRDMEDHNYFVPHRNDLIENYLHNHEFVEVCMKILYQVEL----QRTTLEQMWGFSVEAELIE 1245
            |....|:......|.::...:|          ..|.|..::|    .::..::.|.        :
Human   604 KSLSAMDVEKCTKPCKDSTKKN----------SFKKLLSMKLSICFMKSDFQKFWS--------K 650

  Fly  1246 NAERQDELCCYVSRVEDKSVAMH-NGIIKGDEIMVINGAIVSDLDMMYLESVLQEEQSLSMM--M 1307
            :::..|....::|..|.|.:... .|::.|:|           .....:::...|..||...  .
Human   651 SSQLGDTTTGHLSSGEQKGIESDWQGLLVGEE-----------KRSKPIKAYSTENYSLESQKKR 704

  Fly  1308 RSSRTEPPDLVGIMRVTDDMIDSLVCPPPPTDPPVMSEEMITGLIVPAPGWNGTSKDLYSPEAES 1372
            :.||.:.....|:.  .:.:.|.::.....:..|..|   :|.|.  ||.:.........||.|:
Human   705 KKSRGQTSAANGLR--AESLDDQMLSRESSSQAPYKS---VTSLC--APEYENIRHYEEIPEYEN 762

  Fly  1373 SP---------------ATSFVDPAAMA-----------AQLAVGGVGGVVVGGLGVAKP----- 1406
            .|               ::|..|..|..           .||.:|              |
Human   763 LPFIMAIRKTQELEWQNSSSMEDADANVYEVEEPYEAPDGQLQLG--------------PRHQHS 813

  Fly  1407 TSRTSSFEIENL----LKTAEQETRKSSPTGSVTSSVSTTALTPSRQLTDAE-----KLRKVVME 1462
            :|..|..|..:|    |.:.|:|...||....|:|..|.....|.....|.:     |:..:..|
Human   814 SSGASQEEQNDLGLGDLPSDEEEIINSSDEDDVSSESSKGEPDPLEDKQDEDNGMKSKVHHIAKE 878

  Fly  1463 LVDTERTYVKHLNNLLEHYLEPMKR------ETFLSNAEINALFGNIHEIVTFQRQFLQNLEESL 1521
            ::.:|:.:|..|..|...:.:.:..      :..:.:..:|.:...:.::....|..|:.|||
Human   879 IMSSEKVFVDVLKLLHIDFRDAVAHASRQLGKPVIEDRILNQILYYLPQLYELNRDLLKELEE-- 941

  Fly  1522 DLEPDFNKFEHCGQFRNVLFAIGSAFLYYVNHFKLYSSFCASHSKAQKVLHPNEGNHALQEFLAA 1586
                   :..|..:.:.    |...|:....:.|:||::.....|          |.||.:....
Human   942 -------RMLHWTEQQR----IADIFVKKGPYLKMYSTYIKEFDK----------NIALLDEQCK 985

  Fly  1587 RNP------KQQHSS------TLESYLIKPIQRILKYPLLL-QQMRNLTDTRADEHVHLCEALKG 1638
            :||      ::...|      .|:.||:||:|||.:|.||| ..::||.:. |.::....:||..
Human   986 KNPGFAAVVREFEMSPRCANLALKHYLLKPVQRIPQYRLLLTDYLKNLIED-AGDYRDTQDALAV 1049

  Fly  1639 MEKVAEHINEMQRIHEEYGAIFDHLFR-QHQKSCKQPIDLSPGDLLYYGGVEWLNISDFLGKIKK 1702
            :.:||.|.|:..:    .|..|..|.: |:..:....| :.||.:....|:        |.|:.:
Human  1050 VIEVANHANDTMK----QGDNFQKLMQIQYSLNGHHEI-VQPGRVFLKEGI--------LMKLSR 1101

  Fly  1703 GLELHAMCFVFKSAVVFLC-----KERLRQKKKLMGVSSKNATNEVEIIRYQVLIPVTEVQ---- 1758
            .:....|.|:|..|:::..     ..:|.....|.|:..:..|.|.    ||..:.:..|:
Human  1102 KVMQPRMFFLFNDALLYTTPVQSGMYKLNNMLSLAGMKVRKPTQEA----YQNELKIESVERSFI 1162

  Fly  1759 VRASSAKDMDSHFLW-ELIH--LRSQLQRR----------------SEKVYVL------------ 1792
            :.||||.:.|.   | |.|.  :....::|                .|:|..|
Human  1163 LSASSATERDE---WLEAISRAIEEYAKKRITFCPSRSLDEADSENKEEVSPLGSKAPIWIPDTR 1224

  Fly  1793 --------SNSTADFRNAFLKTIRQIIRESVRNMSIPMKNFGGSSGSVSGHSSQGMGSM------ 1843
                    |..|..:|....:...:|:.::..:....:.........|..|..|.:..:
Human  1225 ATMCMICTSEFTLTWRRHHCRACGKIVCQACSSNKYGLDYLKNQPARVCEHCFQELQKLDHQHSP 1289

  Fly  1844 --GYPGNSQTLERPKQQI-TIVHGSHTLGKPKKKSGSQRHSAGNIDYDNLSG 1892
              |.|||.::   |...: :::|...:..|.||...:.:..:.|.:..::||
Human  1290 RIGSPGNHKS---PSSALSSVLHSIPSGRKQKKIPAALKEVSANTEDSSMSG 1338

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937	21/134 (16%)
		PH	873..974	CDD:278594	19/110 (17%)
		TIAM1_RBD	1141..1219	CDD:176413	14/79 (18%)
		PDZ_signaling	1221..1308	CDD:238492	13/93 (14%)
		RhoGEF	1456..1647	CDD:238091	44/209 (21%)
		PH2_Tiam1_2	1650..1823	CDD:269957	38/221 (17%)
		PH	1693..1812	CDD:278594	30/166 (18%)
FGD6	NP_060821.3	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..36
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	330..351		5/30 (17%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	516..538		4/21 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	695..739		7/45 (16%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	800..869		18/82 (22%)
		RhoGEF	875..1059	CDD:279015	44/207 (21%)
		PH1_FGD6	1077..1199	CDD:275436	27/137 (20%)
		PH	1092..1183	CDD:278594	23/105 (22%)
		FYVE_FGD6	1217..1277	CDD:277282	6/59 (10%)
		PH	1336..1426	CDD:278594	2/3 (67%)
		PH2_FGD5_FGD6	1336..1424	CDD:270057	2/3 (67%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	1	0.910