DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sif and mcf2a

DIOPT Version :9

Sequence 1:	NP_001261446.1	Gene:	sif / 43892	FlyBaseID:	FBgn0085447	Length:	2734	Species:	Drosophila melanogaster
Sequence 2:	XP_021328504.1	Gene:	mcf2a / 557201	ZFINID:	ZDB-GENE-030616-52	Length:	1111	Species:	Danio rerio

Alignment Length:	1229	Identity:	240/1229 - (19%)
Similarity:	421/1229 - (34%)	Gaps:	406/1229 - (33%)

- Green bases have known domain annotations that are detailed below.

  Fly   925 DGAIMQPIPEHPKRDYIFCLSTAFGD----------AYLFQAP------------CQVELENWVN 967
            |.:::..:||        |  :||.|          :||...|            ....|:.| .
Zfish    60 DSSVIITLPE--------C--SAFSDIPEEALAKVLSYLTLIPRARQPGVKFIIILDRRLDTW-T 113

  Fly   968 SIHSA---CAAAFARHRGKTGTLHLL---------QEEI----FRLEKAIESDHKLKHMAELQQS 1016
            ||.:|   .||:|      .|.|||:         |..:    ||..   :.|..|| |..:..|
Zfish   114 SIKTALARIAASF------PGNLHLVLVLRPTSFFQRTVTDLGFRFS---QEDFMLK-MPVVMLS 168

  Fly  1017 VVTDQETRHQIQTQIL-QWEENLERLHCEQFRLRCYMAS------------------LQSGELPN 1062
            .|||. .|:..:.|:. ::...|:..|.:...||..:.|                  |...:|..
Zfish   169 SVTDL-LRYIDENQLTSEFGGTLDYCHSDWIVLRTAIESFAVMVKDIAQMLQAFGTELAETQLSE 232

  Fly  1063 PKS-----LLTHVS--RPTKNTLNKLGVFTVSSFHAFICARSPSLLNNLLAGRGATKRRPPMLSR 1120
            ..|     ||:|..  |..|:.:..            :......||:||
Zfish   233 ECSAIEFLLLSHTEKYRRLKDAIRS------------VMREGRQLLSNL---------------- 269

  Fly  1121 SNSGSSRRSMQMNSR-------DEPEKTF-KVAMPDNAYSTVYLRDAMSVEEFL----------A 1167
               .:||:...:::|       |..::.. ::...:.|:...:.:..:.::::|          .
Zfish   270 ---ETSRKEGDVDTRWDITQDWDTMQRLLAQLTDMEIAFDGFFDKHHLKLQQYLQLLRYEHSFQE 331

  Fly  1168 SACA--------------RRNLNPMEHFVR----VKKRRDMEDHNYFVPHRNDLIENYLHNHE-- 1212
            ..|:              ..:||..|..||    :.||...|     :.....||   ||.|:
Zfish   332 MECSLEKLRAQERKISIIGESLNQTEQSVRELDGLDKRAQEE-----MSRAQVLI---LHGHQLA 388

  Fly  1213 ---------FVEVCMKILYQVELQRTTLEQMWGFSVEAEL----IENAER------------QDE 1252
                     .::.|.::.:|.:...:.|........:|:.    :|.|:|            |.|
Zfish   389 AGHHYAMALIIQRCNELRHQCDTLTSALSTKRSSLTQAQTLLHRLEEAQRWCDDGAYLLANQQVE 453

  Fly  1253 LC-C------YVSRVEDKSVAMHNGIIKGDEIMVIN-GAIVSDLDMMYLESVLQEEQSLSMMMRS 1309
            .| |      .:..:|....|....:..|.:::.:. .::::.....::|...|:..|:..:::|
Zfish   454 KCQCKEGAQTALRDIEKFQEAAPPLLTAGADVLFLEYESVLTPYLQSHIEKTFQKYSSVQALIQS 518

  Fly  1310 SRTEPPDLVGIMRVTDDMIDSL-VCPPPPTDPPVMSEEMITGLIVPAPGWNGTSKDLYSPEAESS 1373
            .:      :.:.::.|..|..: :..|.|.:||                  .:...|:||:.:.:
Zfish   519 RQ------ICLKKMADKHIRPIQLVAPRPENPP------------------RSKSPLFSPKHDFN 559

  Fly  1374 PATSFVDPAAMAAQLAVGGVGGVVVGGLGVAKPTSRTS--SFEIENLLKTAEQETRKSSPTGSVT 1436
            ....|      ...|.:.|            |.|||.|  |.:||    ......|.||...|:.
Zfish   560 SGLKF------TFDLPLPG------------KRTSRKSPNSRKIE----VIHDYQRSSSLPYSID 602

  Fly  1437 SSVSTTALTPSRQLTDAEKLRKVVMELVDTERTYVKHLNNLLEHYLEPMKR-------ETFLSNA 1494
            ...|:..|.           |.|:.||::|||.||:.|..:|..|...|..       .|.|.|.
Zfish   603 GEDSSDLLK-----------RHVMKELIETERIYVEELLAVLLGYRAEMDNPSLAPLLPTSLRNK 656

  Fly  1495 EINALFGNIHEIVTFQRQ-FLQNLEESLDLEPDFNKFEHCGQFRNVLFAIGSAFLYYVNHFKLYS 1558
            . :.||||:.:|..|... |||:||..|: .|:               |:|:.||...:||.||.
Zfish   657 R-DVLFGNLPDIYNFHSSVFLQDLESCLE-SPE---------------AVGACFLERKDHFHLYE 704

  Fly  1559 SFCASHSKAQKVLHPNEGNHALQEFLAARNPKQQHSSTLESYLIKPIQRILKYPLLLQQMRNLTD 1623
            .:|.:..:::.:..........||.    ..|.:|...|:|||:|||||:.||.|||::
Zfish   705 CYCQNKPRSESLWRQFSDCPFFQEC----QKKLEHKLALDSYLLKPIQRLTKYQLLLKE------ 759

  Fly  1624 TRADEHVHLCEALKGMEKVAEHINEMQRIHEEYGAIFDHLFRQHQKSCKQPIDLSPGDLLYYGGV 1688
                    |.:...|.|:|:|....::.:.:...::.|.:   ||.:    |....|||...|.|
Zfish   760 --------LLKYSSGCERVSELQGALEAMLDLLKSVNDSM---HQIA----ITGYEGDLSDLGRV 809

  Fly  1689 -------EWLNISDFLGKIK-----KGLELHAMCFVFKSAVVFLCKERLRQKKKLMGVSSKNATN 1741
                   .|:.......::|     |.::.|  .|:.:.|::| ||.|...        .:|| :
Zfish   810 LMQGSFSVWIGHKKGPTRVKEMARFKPMQRH--LFLHEHALLF-CKRREEH--------GENA-D 862

  Fly  1742 EVEIIRYQVLIPVTEVQVRASSAKDMDSHFLWELIHLRSQLQRRSEKVYVLSNSTADFRNAFLKT 1806
            :.....::..:.::.|.:..:...|:....:|         ....|:|||:...|.:.:.|:|..
Zfish   863 KTPSYSFKHCLKMSAVGITENVKGDVKKFEIW---------YSGREEVYVIQAPTVEVKIAWLNE 918

  Fly  1807 IRQIIRESVRNM------------------SIPM------KNFGGSSGSVSGHSS---------Q 1838
            ||:|:....:.:                  |.|:      :....|.|:.|||||         |
Zfish   919 IRKILTNQQKQLKEELCLSTAVSEQMQMQISPPLSVSKQQRASVSSDGTESGHSSPDAFSCSSQQ 983

  Fly  1839 GMGSMGYPG---------------NSQTLERP-----KQQITIVHGSHTLGKPKKKSG------- 1876
            ......:||               .:.:|..|     :..:.:|.|.:.:....:.:|
Zfish   984 QRNRRSWPGAPYSVDICEGLEDWSGANSLSNPSDTEEEDAVHLVPGRYKVFMDWRNNGLDNLMIK 1048

  Fly  1877 -------SQRHSAGNIDYDNLSGSQEADDLPPSVGVVHYASGHTHGQQMQPAGFRGRSK 1928
                   ......|.....|||..||.....|::..:...|...|..::...|.|...|
Zfish  1049 CGDVIQLQHEEDHGQWLVKNLSRRQEGRVPTPNLQCILGESMKNHSSRLGDQGIRKARK 1107

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937	18/84 (21%)
		PH	873..974	CDD:278594	15/73 (21%)
		TIAM1_RBD	1141..1219	CDD:176413	17/117 (15%)
		PDZ_signaling	1221..1308	CDD:238492	16/110 (15%)
		RhoGEF	1456..1647	CDD:238091	61/198 (31%)
		PH2_Tiam1_2	1650..1823	CDD:269957	36/208 (17%)
		PH	1693..1812	CDD:278594	24/123 (20%)
mcf2a	XP_021328504.1	SEC14	46..191	CDD:214706	35/152 (23%)
		SPEC	324..520	CDD:321951	32/203 (16%)
		RhoGEF	611..788	CDD:238091	61/222 (27%)
		PH_Dbs	795..926	CDD:269934	31/155 (20%)
		SH3_DBS	1030..1084	CDD:212791	8/53 (15%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	2	1.910