DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sif and Arhgef5

DIOPT Version :9

Sequence 1:	NP_001261446.1	Gene:	sif / 43892	FlyBaseID:	FBgn0085447	Length:	2734	Species:	Drosophila melanogaster
Sequence 2:	NP_598435.1	Gene:	Arhgef5 / 54324	MGIID:	1858952	Length:	1581	Species:	Mus musculus

Alignment Length:	1617	Identity:	294/1617 - (18%)
Similarity:	501/1617 - (30%)	Gaps:	592/1617 - (36%)

- Green bases have known domain annotations that are detailed below.

  Fly   385 STEDMNVDTSTLKRMLKPMP-----STESPVTSPEMGRRRYNYYNANAAQTLGHPPHMHQHGMAM 444
            |||..:::.      ||.:|     |::.|...||....|...|.    .|.||.|.|:|..:..
Mouse    11 STEVPDIEE------LKTIPEGIMRSSQIPALDPEAQEDRDPSYK----WTDGHRPVMNQSKVLR 65

  Fly   445 GMGGGGGGGHHIMNNNTMGRASSQSSRFSGSRSSHEIGRGYPPRNLYLELERERSCIEGSPPSDN 509
            .||                                    .:.|.::.:..::|.|.:|   .|..
Mouse    66 DMG------------------------------------DHTPNSMAIFFKKESSDME---TSQE 91

  Fly   510 VMFDNQCYATTPSSSNGNSDQDQSYGQQQSSGQHPQQQQGPPQRSSRHQHHHQQAPNVTPTPGSP 574
            ::....|  .||       ||.::..|...            .|.||             |..:|
Mouse    92 ILLAEAC--NTP-------DQQEAVIQSLK------------DRLSR-------------TIAAP 122

  Fly   575 TSRLL--------LEYEMHLRNTLAKGMDAESYSLHTFEALLSQSMENLEFAESLPGSNQRSPYP 631
              .||        |:....|.|.:...:.:|..||     .|:.|.|..|...|...|..|..:|
Mouse   123 --ELLACAVQEEWLDIPSKLDNRVGAELQSELMSL-----TLAVSKEKEEEETSPDTSIPRGSWP 180

  Fly   632 LRRPNAKSSTLPLPPHRPLSTIRDKERDRDRDGYYSDRNELIRE---------RERERDRGYLSD 687
                             |..|...:.......|     :||:|:         :|.:...|:|
Mouse   181 -----------------PCKTHPGETEQTQGSG-----SELLRQGKQLQLEATQENQGQEGFL-- 221

  Fly   688 HNSSFSNSRCASCIGESARAQWFRHSDG-----WRSGSSTIGSGSGHGMMTQQ------IPGSGH 741
                           :|..||.....:|     ...|:...|...| |::.:|      ..|:..
Mouse   222 ---------------QSQEAQGLEEQEGQEVEIQEEGTLNEGICFG-GLLGEQEEVEEGFNGNEE 270

  Fly   742 KR------------SPWDSLPSLRQDSSLNDSGYKSARADSLEQRAEFIRQDSLRSEYLSDRESR 794
            ::            ..|::.....:...||    .|.|.....:|..::.:||  .|...|:|||
Mouse   271 EQKQGQIQSYMLLGGQWENEGLSGELEGLN----YSERGQENRERRVWVLRDS--EEEGQDQESR 329

  Fly   795 YGIVQQASIESTDSRMCYLTSSEI-----SDDDRMSLTTAVSDE----DDGESVMASPYKAKATG 850
            ....::.:.:.|:::. .:..|||     .|.|:......|.|:    |.|:.|..:
Mouse   330 EVEERRVATQYTENQR-LVEKSEIVKRKQRDHDQTGKVMPVRDQKEVVDSGDRVQGN-------- 385

  Fly   851 TAASSFNCTGAVRKAGFLSVKKWLLRKKHQIELARKRGWKGYWVCLKGTTLLFYPCDSREGR-SV 914
                                                 |..|....::|         ||.|. |.
Mouse   386 -------------------------------------GDSGGQTAVEG---------SRPGEDSK 404

  Fly   915 EAAPKHLIIVDGAIMQP-------------IPEHPKRDYIFCLSTAFGDAYLFQAPCQVELENWV 966
            .:.|  :..||..::.|             ||:..|......||.        |||. :|...|.
Mouse   405 PSLP--VASVDPEVLSPGTLFPGISSSVADIPQIQKEPVCEELSP--------QAPA-LEPTEWS 458

  Fly   967 NSIHSACAAAFARHRGKTGTLHLLQEEIFRLEKAIESDHKLKHMAE---------LQQSVVTDQE 1022
            :...|. .|:||.........|..|:|.|||.|.||.......:|.         ...:|.:...
Mouse   459 HQPISP-PASFAPEESLDNRTHNSQQEEFRLRKGIEVVSASTSVAPSGTRDSPPFSPPNVFSSTA 522

  Fly  1023 TRHQIQTQILQWEENLERL-------HC----------EQFRLRCYMASLQSGELPNPKSLLT-- 1068
            |...:.:.::..||.....       ||          :..|..|     .:.:..||.|.|:
Mouse   523 TLSPVSSSVILPEETPTASASADTPHHCGPCETPPLPAKSSRYPC-----ATSDTANPHSPLSSY 582

  Fly  1069 -----HV-------SRPTKNTLNKLGVFTVSSFHAFICAR--SPSLLNNLLAGRGATKRRPPMLS 1119
                 |:       |..|:.|.:.||: ::|..|..:..|  .|::..:|...|  .:|....:.
Mouse   583 TGVTQHLRSNSFPGSHRTEQTPDSLGM-SLSFSHLELPQRPPKPAIYGSLTPRR--NRRSRDGIV 644

  Fly  1120 RSNSGSSRRSMQMNSRD---EPEK----------------TFKVAMPDNAYSTVYLRDAMSVEEF 1165
            .|:|.::..:::.:|.:   .||:                .|.:..|.|..|...:...|::.|.
Mouse   645 FSDSSTALFALKQDSEEFTSNPERPSSPHGSPTWGSPQNSAFAIGSPANVSSPPTVSMDMTIREA 709

  Fly  1166 LASACARRNLNPMEHFVRVKKRRD---------MEDHNYFVP-------HRN---------DLIE 1205
            |......:. :...|.|    .||         ::.|::..|       ||:         |...
Mouse   710 LLPIPPEKR-HSYSHIV----ERDGLLHEVASTLKRHSHPPPLTLSSGLHRSSKGSFSLVPDSTV 769

  Fly  1206 NYLH----------NHEFVEVCMKILYQVELQRT-TLEQMWGFSVEAELIENAERQDELCCYVSR 1259
            ...|          ||....:..::.|...|..| .:.:.:..|:.:.             |:||
Mouse   770 ARQHRPLPSTPESPNHTQTSIPSRLRYNKPLPPTPDMPEFYHPSISSS-------------YISR 821

  Fly  1260 V-----------------------EDKSVAMHNGII-KGDEIMVINGAIVSDLDMMYLESVL--- 1297
            :                       ..:|.::..|:| .|.:      |.....:..:..|.|
Mouse   822 MYRPLPPVPIIDPSSEPPPLPPKSRGRSKSIQGGVIHSGGQ------AKPRPNNQDWTASTLSVG 880

  Fly  1298 ---------QEEQSLSMMMRSSRTEPPDLVGIMRVTDDMIDSLVCPPPPT--------------- 1338
                     :..:||.:..|.:....|.| ....:|     :|:.|..||
Mouse   881 RTSWPPATGRSTESLPLTSRCNNEVSPGL-AFSNMT-----NLLSPSSPTTPWIPDLQRPTTKDE 939

  Fly  1339 -------DPPV-----------------------------------------------------M 1343
                   :|||                                                     .
Mouse   940 SGLTEESEPPVRGSFRRSAPQEEFNNTRRSALGSRKNSEKPLHHQLEKASSWPHRRDPARTSESS 1004

  Fly  1344 SEEMITGLIVP--APGWN-----------GTSKDLYSPEAESSPATSFVDPAAMAAQLAVGGVGG 1395
            ||:::.|. ||  ..|||           .:|.||.:|.|...|.:|  |......:....|:||
Mouse  1005 SEQVVLGQ-VPNKQKGWNRQGLRRPSILPESSSDLRNPAAGRLPGSS--DSVVFREKKPKEGMGG 1066

  Fly  1396 --------------------VVVGGL--------GVAKPTSRTSSFEIENLLKTAEQETRKSS-- 1430
                                ||:...        .:|:|...:|.......|.:::...::.|
Mouse  1067 FSRRCSKLISSQLLYQEYSDVVLNKEIQSQQRLDSLAEPHGLSSPRHRRKALVSSDSYLQRLSMA 1131

  Fly  1431 PTGSVTSSV-----STTALTPSRQLTDAEKLRKVVMELVDTERTYVKHLNNLLEHYLEPMKRETF 1490
            .:||:...:     ||..|:.:.   :.:||::...||:.:|.:|::.||..::|:....:....
Mouse  1132 SSGSLWQEIPVVRNSTVLLSMTH---EDQKLQEAKFELIVSEASYLRSLNIAVDHFQHSAQLRGT 1193

  Fly  1491 LSNAEINALFGNIHEIVTFQRQFLQNLEESLDLEPDFNKFEHCGQFRNVLFAIGSAFLYYVNH-- 1553
            |||.:...||..:.::......||.:|||  :.|.:...|:.|....|........:|.||.:
Mouse  1194 LSNQDHQWLFSRLQDVRDVSTTFLSDLEE--NFENNIFSFQVCDVVLNHAADFHRVYLPYVTNQT 1256

  Fly  1554 --FKLYSSFCASHSKAQKVLHPNEGNHALQEFLAARNPKQQHSSTLESYLIKPIQRILKYPLLLQ 1616
              .:.:.|...|:|..::||...|.:...|..            :|:|:||.|.|||.:..||||
Mouse  1257 YQERTFQSLMNSNSSFREVLEKLESDPICQRL------------SLKSFLILPFQRITRLKLLLQ 1309

  Fly  1617 QMRNLTDTRADEHVHLCEALKGMEKVAEHIN-EMQRIHEEYGAIFDHLFRQHQKSCK 1672
            .:...|...:.|.....:|...:||:....| .:||:......|:  |.::.:..||
Mouse  1310 NILKRTQPGSSEEAEATKAHHALEKLIRDCNSNVQRMRRTEELIY--LSQKIEFECK 1364

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937	25/138 (18%)
		PH	873..974	CDD:278594	22/114 (19%)
		TIAM1_RBD	1141..1219	CDD:176413	19/112 (17%)
		PDZ_signaling	1221..1308	CDD:238492	16/123 (13%)
		RhoGEF	1456..1647	CDD:238091	49/194 (25%)
		PH2_Tiam1_2	1650..1823	CDD:269957	6/23 (26%)
		PH	1693..1812	CDD:278594
Arhgef5	NP_598435.1	ARHGEF5_35	1..477	CDD:373847	120/668 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	25..54		7/32 (22%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	159..274		26/154 (17%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	316..643		78/403 (19%)
		PHA03247	<398..949	CDD:223021	104/600 (17%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	659..700		7/40 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	741..810		11/68 (16%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	829..1051		35/236 (15%)
		RhoGEF	1164..1340	CDD:366202	48/189 (25%)
		PH_ephexin	1362..1488	CDD:269929	2/3 (67%)
		SH3_ARHGEF5_19	1498..1552	CDD:212873
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	0.910