DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sif and net1

DIOPT Version :9

Sequence 1:	NP_001261446.1	Gene:	sif / 43892	FlyBaseID:	FBgn0085447	Length:	2734	Species:	Drosophila melanogaster
Sequence 2:	XP_021330686.1	Gene:	net1 / 493607	ZFINID:	ZDB-GENE-041121-18	Length:	605	Species:	Danio rerio

Alignment Length:	617	Identity:	128/617 - (20%)
Similarity:	220/617 - (35%)	Gaps:	162/617 - (26%)

- Green bases have known domain annotations that are detailed below.

  Fly  1352 IVPAPGWNGTSK----DLYSPEAES-------SPATSFVDPAAMAAQLA-------VGGV----- 1393
            :.|.|.|:.|.|    :....:|.|       .|.|..|.|......||       .|.|
Zfish    53 LTPGPQWDFTLKRKRREKDDADAVSLCSFDFKEPCTKRVRPLGRVTSLANLISPVKNGAVRRFGQ 117

  Fly  1394 -------GGVVVGGLGVAKPTSRTSSFEIENLLKTAEQETRKSSPTGSVTSSVSTTALTPSRQLT 1451
                   |.....|:...||.|:.::         .....|::|...|.|..|.......::::
Zfish   118 TLQASFRGDGRSPGVPQQKPCSKAAA---------PTPPKRRNSTLWSETLDVHQKGTFSTKEI- 172

  Fly  1452 DAEKLRKVVMELVDTERTYVKHLNNLLEHYLEPMKRETFLSNAEINALFGNIHEIVTFQRQFLQN 1516
               |.::.:.||...|:..::.|....:.|.:||.:.:.:|..|:.|:||::...:......|..
Zfish   173 ---KRQEAIFELSRGEQDLIEDLKLARKAYHDPMLKLSIMSEEELTAIFGDLDAYIPLHEDLLDQ 234

  Fly  1517 LEESLDLEPDFNKFEHCGQFRNVLFAIGSAFLYYVNHFKLYSSFCASHSKAQKVLHPNEGNHALQ 1581
            |.::...:         |..|.    ||...:.::.....|.::|::...|:.:|...:.:..:|
Zfish   235 LAKATGTD---------GTVRQ----IGKIVVDWLPRLNAYRAYCSNQLAAKALLDQKKQDPRVQ 286

  Fly  1582 EFLAARNPKQQHSSTLE--SYLIKPIQRILKYPLLLQQMRNLTDTRADEHVHLCEALKGMEKVAE 1644
            :|| .|..:...|..|:  |:|..|..|::||||||:::...|.....:...|.:|:..::.|..
Zfish   287 DFL-QRCLESPFSRKLDLWSFLDIPRSRLVKYPLLLKEILRHTPPDHPDKGSLEQAISVIQAVLA 350

  Fly  1645 HINEMQRIHEEYGAIFDHL--FRQHQK-----SCKQPIDLSPGDLLYYGGVEWLNISDFLGKIKK 1702
            .|| |::...|.....|.|  ....||     .||        .||.:|.:          :.|.
Zfish   351 DIN-MKKGESECQYYIDKLEYLEDKQKDPRIEQCK--------SLLCHGEL----------RNKS 396

  Fly  1703 GLELHAMCFVFKSAVVF-----------------------LCKERLRQKKKLMGVSSK----NAT 1740
            |.:||.  |:|...::.                       |..|.|:.....||.|.:    ||.
Zfish   397 GTKLHV--FLFTEVLILTRPVTRNDRHCFQVYRQPIPVQDLVLEDLQDGDVRMGGSFRGAFSNAD 459

  Fly  1741 NEVEIIRYQVLIPVTEVQVRASSAKDMDSHFLW--ELIH-------LRSQLQRRSEKVYVLSNS- 1795
            ....|.|           ||...|....||.|.  ::.|       ||:.:.  ::|...||.:
Zfish   460 KAKNIFR-----------VRFQDASQGQSHTLQVNDVFHKQQWLNCLRNAMS--TQKDLTLSENE 511

  Fly  1796 ---------TADFRNAFLKTIRQI-------IRESVRNMSIPMKNFGGS---SGSVSGHSSQGMG 1841
                     .|..|::.:.|:.|:       .|.:....|.|......|   |.:.:..||....
Zfish   512 PLTTPATDVCAKRRSSSVSTVIQMDEMDENCPRAAASAPSSPSSEEPPSPTPSATSTLSSSSSSS 576

  Fly  1842 SMGYPGNSQTLERPKQQITIVHGSHTLGKPKK 1873
            |:..|..|:..::.|:.:.      .|||.|:
Zfish   577 SISAPIPSRKSKKDKRSLC------PLGKRKE 602

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937
		PH	873..974	CDD:278594
		TIAM1_RBD	1141..1219	CDD:176413
		PDZ_signaling	1221..1308	CDD:238492
		RhoGEF	1456..1647	CDD:238091	42/192 (22%)
		PH2_Tiam1_2	1650..1823	CDD:269957	45/232 (19%)
		PH	1693..1812	CDD:278594	32/171 (19%)
net1	XP_021330686.1	RhoGEF	177..353	CDD:214619	42/189 (22%)
net1	XP_021330686.1	PH-like	367..500	CDD:327399	33/163 (20%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	1	0.910