Protein Alignment sif and pbl

DIOPT Version :9

• Sequence 1: NP_001261446.1
• Gene: sif / 43892
• FlyBaseID: FBgn0085447
• Length: 2734
• Species: Drosophila melanogaster
• Sequence 2: NP_523965.2
• Gene: pbl / 38879
• FlyBaseID: FBgn0003041
• Length: 1311
• Species: Drosophila melanogaster
• Alignment Length: 1717
• Identity: 324/1717 - (18%)
• Similarity: 522/1717 - (30%)
• Gaps: 689/1717 - (40%)

  Fly   238 VSSTSLYDNVASGGPGTNQGADTLPRQMKGGQQDRQDVANSGVNTNTPGVIVTGVGNVGSDMCGQ 302
            :|.|:|...:...| |..|.||||           |...:.|:      .|||  .:.|.|:.|:
  Fly    14 MSITTLPTRICLVG-GVGQDADTL-----------QAAESFGL------PIVT--SDTGLDILGE 58

  Fly   303 N--------------------HVGSQVGNDDPA---ACQMDMDLSKSEGTQAGGGLHQSVGTCTS 344
            :                    |...:.....||   |.:|...|.::........: :.|.||.
  Fly    59 SSDWRTFYVLDDFEGASFEAIHKQKECILGPPALKYAAEMKQTLGQNSRPIYNYAM-RGVVTCF- 121

  Fly   345 SSKGTGTRNKDFGDDMTRDAHS------HDMH---QHNVINNNTRRKTKSTEDMNV--------- 391
                ||.|.||....:....||      .|::   .|.:.|::...|.:..:...:
  Fly   122 ----TGIRKKDELTKLVNLIHSMGGCIKKDLNTKTTHLICNHSGGEKYQYAKTFRLTVVRPAWVF 182

  Fly   392 ----DTSTLKRMLKPMPSTESPVTSPEMGRRRYNYYNANAAQTLGHPPHMHQHGMAMGMGGGGGG 452
                |.::|:     ..:|:...|.    ..|...:........|.|...|||.:.:.:..||..
  Fly   183 AAWADRNSLE-----FDATQENFTK----THRLKAFEGQKICFFGFPVEEHQHMVDVLLENGGVC 238

  Fly   453 GH-------HIMNNNTMGRASSQS---------------SRFSGSRSSHEIG------------- 482
            ..       |::..||....:|.|               |:..||.|:.:.|
  Fly   239 AELDDPECSHVVMPNTGAVFTSTSTSTDTNPSTLINPPESQVPGSSSASQAGEEEQRKAEHLEQA 303

  Fly   483 --------RGYPPRNLYLELERERSCIEGSPPSDNVMFDNQCYATTPSSSNGNSDQDQSYGQQQS 539
                    .......:.|::::|:...:|....|::.||.:.:.......|...||     ..:|
  Fly   304 EIKKMEYSHAETDNGMSLDIDQEQKENQGIEDEDDIHFDERLFVEAADIINHGEDQ-----LPES 363

  Fly   540 SGQHPQQQQGPP-QRSSRHQHHHQQAPNVTPTPGSPTSRLLLEYEMHLRNTLAKGMDAESYSLHT 603
            ..:..:::|.|. :...:.|...:.....||    .||::                   ..||..
  Fly   364 DEEDLEEEQKPKVEEVLKEQEVEEDVQASTP----KTSKI-------------------RTSLKA 405

  Fly   604 FEALLSQSMEN--LEFAESLPGSNQRSPYPLRRPNAKSSTLPLPPHRPLSTIRDKERDRDRDGYY 666
            ...|.|..|:.  |:|                    :|:|..:.|  .|..|  .|.|.:.|...
  Fly   406 ATPLSSPEMDGIALDF--------------------ESTTQNISP--ILKAI--DECDLETDDLP 446

  Fly   667 SDRNELIRERERERDRGYLSDHNSSFSNSRCASCIGESARAQWFRHSDGWRSGSSTIGSGSGHGM 731
            ...|:|.|:||             ||.|....|.             |.:
  Fly   447 PSENDLKRKRE-------------SFDNVSLMSV-------------DSF--------------- 470

  Fly   732 MTQQIPGSGHKRSPWDSLPSLRQDSSLNDSGYKSARADSLEQRAEFIRQDSLRSEYLSDRESRYG 796
               .:|.|..|       |.|.:..|:         :.:|.:...|:.:..:::.    |..|..
  Fly   471 ---ALPASTKK-------PKLIRTGSI---------SRTLRRSVSFVAEPLMKTL----RPRRSS 512

  Fly   797 IVQQASI---ESTDSRMCYLTSSEISDDDRMSLTTAVSDEDDGESVMASPYKAKATGTAASSFNC 858
            :...:|.   |:.|:.:|.|.||       ::||  ::|.      :..|.|.|     ..|| |
  Fly   513 VADASSFVGSEAADNSICSLASS-------INLT--LNDS------IRKPVKEK-----LRSF-C 556

  Fly   859 TGAVRKAGFLSVKKWLLRKKHQIELARKRGWKGYWVCLKGTTLLFYPCDSREGRSVEAAPKHLII 923
            ....|.:.          ::|.      ||..|...|   ||||    ||  |.....|||
  Fly   557 GRITRSSS----------RRHD------RGVDGTPEC---TTLL----DS--GFKTPKAPK---- 592

  Fly   924 VDGAIMQPIPEHPKRDYIFCLSTAFGDAYLFQAPCQVELENWVNSIHSACAAAFARHRGKTGTLH 988
               ......|:..||        .||.               |:.:.||.
  Fly   593 ---LRSTATPKTAKR--------LFGP---------------VSGVVSAL--------------- 616

  Fly   989 LLQEEIFRLEKAIESDHKLKHMAELQQSVVTDQETRHQIQTQILQWEENLERLHCEQFRLRCYMA 1053
                                        .:|:..|
  Fly   617 ----------------------------TLTNPST------------------------------ 623

  Fly  1054 SLQSGELPNPKSLLTHVSRPTKNTLNKLGVFTVSSFHAF-ICARSPSLLNNLLAGRGATKRRPPM 1117
                 ..|||        .|..|....|.| ..:|.|.| .||.:            .|...||
  Fly   624 -----PTPNP--------NPNTNCTATLDV-NDTSIHTFAFCAAA------------VTPAMPP- 661

  Fly  1118 LSRSNSGSSRRSMQMNSRDEPEKTFKVAMPDNAYSTVYLRDAMSVEEFLASACARRNLNPMEHFV 1182
             |.|:|.||           |..:..:|:    :|:|.:  ..|.||   |...|.:..|:|..:
  Fly   662 -SSSSSSSS-----------PILSTSIAV----HSSVQV--IKSPEE---STTRRNSPAPIEEPM 705

  Fly  1183 RVKKRRDMEDHNYFVPHRNDLIENYLHNHEFVEVCMKILYQVELQRTTLEQMWGFSVEAELIENA 1247
            .:.    :::|                                   |||
  Fly   706 AMV----VDEH-----------------------------------TTL---------------- 715

  Fly  1248 ERQDELCCYVSRVEDKSVAMHNGIIKGDEI-MVINGAIVSDLDMM---YLESVLQEEQSLSMMMR 1308
                      ::.|.|:  .|..|:|.|.. ..|.....:::|.:   ||:|:
  Fly   716 ----------TKPEPKN--NHTHILKSDWFWYTIQNGYANEMDYLFGDYLDSI------------ 756

  Fly  1309 SSRTEPPDLVGIMRVTDDMIDSLVCPPPPTDPPVMSEEMITGLIVPAPGWNGTSKDLYSP--EAE 1371
               |..|:        .|..|||         |:              .:|...:..:|.  :.|
  Fly   757 ---TNTPN--------TDRRDSL---------PI--------------SFNKRKRKRFSQRIQLE 787

  Fly  1372 SSPATSFVDPAAMAAQLAVGGVGGVVVGGLGVAKPTSRTSSFEIENLLKTAEQETRKSSPTGSVT 1436
            .:|                          ||..|   |.||.....||              ||:
  Fly   788 GTP--------------------------LGSGK---RRSSVSDAGLL--------------SVS 809

  Fly  1437 SSVSTTALTPSR-----------QLTDAEKLRKVV-----MELVDTERTYVKHLN---NLLEHYL 1482
            :|:.....:|.:           :.:||...:|.:     |:...||..||..|:   ||.::.|
  Fly   810 NSLFDCTTSPDKLESDKLLHAEPEASDATPAKKSMRFNHFMDFYTTESNYVGILDTILNLFKNKL 874

  Fly  1483 EPM--KRETFLSNAEINALFGN---IHEIVTFQRQFLQNLEESLDLEPDFNKFEHC--G----QF 1536
            |.:  ..:..|:.:||..:|||   |||:.....:.|:.|..        |..|.|  |    |.
  Fly   875 EELAETNDPLLNKSEIKTIFGNFLPIHEVHQSMLEHLRKLHA--------NWREDCLIGDIIIQH 931

  Fly  1537 RNVLFAIGSAFLYYVNHF---KLYSSFCASHSKAQKVLHPNEGNHALQEFLAARNPKQQ-HSSTL 1597
            |:.|.   .|:..|||.|   |....:|...       :|.  .||   ||.....|.: ....|
  Fly   932 RDELI---KAYPPYVNFFEQMKEQLQYCDRE-------YPR--FHA---FLKINQTKPECGRQGL 981

  Fly  1598 ESYLIKPIQRILKYPLLLQQMRNLTDTRADEHVHLCEALKGMEKVAEHINEMQRIHEEYGAIFDH 1662
            :..:|:|:||:....|||..:...|.:...:|..|.||||.:::|..||||.:|..|...||||
  Fly   982 QDLMIRPVQRLPSISLLLNDILKHTTSGNADHGRLEEALKAIKQVTLHINEDKRRTESRMAIFD- 1045

  Fly  1663 LFRQHQKSCKQPIDLSPGDLLYYGGVEWLNISDFLGKIKKGLELHAMCFVFKSAVVFLCKERLRQ 1727
            :|...: .|  |..|...:..:....|...:||.|......|.|    ::|..::. |||.|.:.
  Fly  1046 IFNDIE-GC--PAHLVSSNRSFILKCEVNELSDSLSGRGDSLVL----YLFSDSIE-LCKRRSKG 1102

  Fly  1728 KKKLMGVSSKNATNEVEIIRYQVLIPVTEVQVRASSAKDMDSHFLWELIHLRSQLQRRSEKVY-- 1790
            .......|:......:::|....:..|.::.         ||...:.|:     |:...||:|
  Fly  1103 FNTAKSPSTAKTHKHLKLISLNTIRLVIDIS---------DSPRAFGLL-----LRGDKEKLYTF 1153

  Fly  1791 VLSNSTADFRNAFLKTI-RQIIRESVR 1816
            .:|:...| ::.::|.: .||...:.|
  Fly  1154 TISDEETD-KSVYVKKLCNQIAAHTCR 1179

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937	24/124 (19%)
		PH	873..974	CDD:278594	23/100 (23%)
		TIAM1_RBD	1141..1219	CDD:176413	11/77 (14%)
		PDZ_signaling	1221..1308	CDD:238492	14/90 (16%)
		RhoGEF	1456..1647	CDD:238091	59/213 (28%)
		PH2_Tiam1_2	1650..1823	CDD:269957	37/170 (22%)
		PH	1693..1812	CDD:278594	25/121 (21%)
pbl	NP_523965.2	PTCB-BRCT	117..180	CDD:289507	14/67 (21%)
		RhoGEF	852..1032	CDD:279015	58/202 (29%)
		PH_Ect2	1023..1186	CDD:269936	42/181 (23%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
	1	0.910