DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sif and Arhgef28

DIOPT Version :9

Sequence 1:	NP_001261446.1	Gene:	sif / 43892	FlyBaseID:	FBgn0085447	Length:	2734	Species:	Drosophila melanogaster
Sequence 2:	XP_038958459.1	Gene:	Arhgef28 / 361882	RGDID:	1307061	Length:	1714	Species:	Rattus norvegicus

Alignment Length:	1805	Identity:	356/1805 - (19%)
Similarity:	605/1805 - (33%)	Gaps:	536/1805 - (29%)

- Green bases have known domain annotations that are detailed below.

  Fly   728 GHGMMTQQIPGSGHKRSPWDSLPSLRQDSSLNDSGYKSARADSLEQRAEFIRQDSLR--SEYLSD 790
            ||.|:.:.|.....:|||..|...|.:|::|...........::...||.:.:..::  .:|..|
  Rat   224 GHSMLVEDISNFQGRRSPGFSRLRLNEDATLQFDRSSETLTLTVNHTAEHLLEADIKLFRKYFWD 288

  Fly   791 RESRYGIVQQASIESTDSRMCYLTSSEISDDDRMSLTTAVS---DEDDGESVMASPYKAKATGTA 852
            |..   :|:....|:...|....:.:..::::..:|.:..|   :|:||:.|     |::|.|.:
  Rat   289 RAF---LVKALDQEAKTERATMPSGAAETEEEVRNLESGRSPSEEEEDGQLV-----KSQADGPS 345

  Fly   853 ASSFNCTGAVRKAGFLSVKKWLLRKKH--------QIELARKRGWKGYWVCL----KGTTLLFYP 905
            ... :....|....|..:||    .||        |:......|.:.|..|:    .|...:.|
  Rat   346 EQE-DQDRLVLDHSFDGLKK----SKHAPASLAAGQLSDVPNGGDEVYANCMVIDQVGDLDINY- 404

  Fly   906 CDSREGRSVEAAP------------KHLIIVDGAIMQPIPEHPKRDYIFCLSTAFGDAYLFQAPC 958
             .:.||.|...:|            ||::        | |:.....::.|..:   |..|..|..
  Rat   405 -INIEGLSTHTSPESMRSTLGPQACKHIL--------P-PDTSPCGHLLCEDS---DRTLDAAAS 456

  Fly   959 QVELENWVNSIHSACAAAFARHRGKTGTLHLLQEEIFRLEKAIESDHKLKHMAELQQSVVTDQET 1023
            |            :|.:..:.|   |..|:|    .|.|...::....||..:....::|.|.|.
  Rat   457 Q------------SCMSPPSSH---TSNLNL----SFGLHGFVKEQSHLKKRSSSLDALVADSEE 502

  Fly  1024 RHQIQTQILQWEENLERLHCEQFRLRCYMASLQSGE---LPNPKSLLT---------HVSRPTKN 1076
            ..:.:..|                  ||....||..   ||....|.:         ::||....
  Rat   503 EGRSEPPI------------------CYAVGSQSSPRTGLPGGDELDSFDANTEPDCNISRTESL 549

  Fly  1077 TLNKLGVFTVSSFHAFICARSPSLLNNLLAGRGATKRRPPMLSRSNSGSSR--RSMQMNSRDEPE 1139
            :|:       |:.|:     ..|||:. :..|..:...|    :.:||.||  |...:.|..|.:
  Rat   550 SLS-------STLHS-----KESLLSG-IRSRSYSCSSP----KISSGKSRLVRDFTVCSSSEEQ 597

  Fly  1140 KTFKVAMP-----------DNAYSTVYLRDAMSVEE---------FLAS-ACARRNLNPMEHFVR 1183
            :::....|           |:     |:..|.:..|         ||.: ..:.||.:.|
  Rat   598 RSYSFQEPPGEKRIQEEEWDD-----YIIPAKAESEKHKVSRTFSFLMNRMTSPRNKSKM----- 652

  Fly  1184 VKKRRDMEDHNYFVPHRNDLIENYLHNHEFVEVCMKILYQVE-LQRTTL--EQMWGFSVEAELIE 1245
              |.:|.:|            :..|:.|:||......:.|.. ..:|.|  |.:...:.:|...:
  Rat   653 --KNKDTKD------------KEKLNRHQFVPGTFSGVLQCSGCDKTLLGKESLQCANCKANTHK 703

  Fly  1246 NAERQDELCCYVSRVEDKSVAMHNGIIKGDEIMVINGAIVSDLDMMYLESVLQEEQSLSMMMRSS 1310
            ..:.....|  ..:.:||                                             .:
  Rat   704 GCKDTVPPC--TKKFQDK---------------------------------------------YN 721

  Fly  1311 RTEPPDLVGIMRVTDDMIDSLVCPPPPTDPPVMSEEMITGLIVPAPGWNGTSKDLYSPEAESSPA 1375
            :.:|..::|...|.|        .|||......|..|..||  || |...|:...: |.:.|.|.
  Rat   722 KNKPQTILGSSSVRD--------VPPPGLSLHPSPSMPIGL--PA-GRKETAGQAH-PLSRSVPG 774

  Fly  1376 TSFVDPAAMAAQLAVGGVGG---------VVVGGLGVAKPTSRTSSFEIENLLKTAEQETRKSSP 1431
            |:..........|...|...         .:...:| :.|:  |.||.:|:::.:
  Rat   775 TTLESFRRAVTSLESEGDSNSWRSRSHSDELFQSMG-SSPS--TESFMMEDVVDS---------- 826

  Fly  1432 TGSVTSSVSTTA-----------LTP---SRQLTDAEKLRKVVMELVDTERTYVKHLNNLL---E 1479
              |:.|.:|:.|           :.|   |||..|..|.:.|:.||:.||   |.|:..||   |
  Rat   827 --SLWSELSSDAQEFEAESWSLVVDPSFCSRQEKDVIKRQDVIFELMQTE---VHHIQTLLIMSE 886

  Fly  1480 HYLEPMKRETFLSNAEINALFGNIHEIVTFQRQFLQNLEESLDLEPDFNKFEHC-GQFRN-VLFA 1542
            .:.:.||.|..|.::.::.:|..:.|::...:.|..:::|        .:.|.| |..|| |:..
  Rat   887 VFRKGMKEELQLDHSTVDRIFPCLDELLETHKHFFFSMKE--------RRQESCTGNDRNFVINQ 943

  Fly  1543 IGSAFLYYVNHF---------KLYSSFCASHSKAQKVLHPNEGNHALQEFLAARNPK-QQHSSTL 1597
            ||...   |..|         ::|..||:.|.:|..:....:.|...|.|:..||.. ......:
  Rat   944 IGDIL---VQQFSEENASKMKRIYGDFCSHHKEAMSLFKELQQNKKFQNFIKIRNSNLLARRRGI 1005

  Fly  1598 ESYLIKPIQRILKYPLLLQQMRNLTDTRADEHVHLCEAL---KGM--------------EKVAEH 1645
            ...::...|||.|||:|::::...|..|.:||..||.||   |.|              :|..|.
  Rat  1006 PECILLVTQRITKYPVLVERILQYTKERTEEHRDLCRALGLIKDMIAAVDLKVSEYEKNQKWLEI 1070

  Fly  1646 INEMQRIHEEYGAIFD-HLFRQHQKSCKQPIDLSPGDLLYYGGVEWLNISDFLGKIKKGLELHAM 1709
            :|:::  ::.|..:.: |:||      ||.:......||:.|.|.|...:   |:.|..|.|
  Rat  1071 LNKIE--NKTYTKLKNGHVFR------KQALMSQERALLHDGLVYWKTAT---GRFKDILAL--- 1121

  Fly  1710 CFVFKSAVVFLCKERLRQKKKLMGVSSKNATNEVEIIRYQVLIPVTEVQ--------VRASSAKD 1766
              :....::||  :...||.....|..|.:     :|..|.|| ..||.        :.||||
  Rat  1122 --LLTDVLLFL--QEKDQKYIFAAVDQKPS-----VISLQKLI-AREVANEERGMFLISASSA-- 1174

  Fly  1767 MDSHFLWELIHLRSQLQRRSEKVYVLSNSTADFRNAFLKTIRQIIRESVRNMSIPMKNFGGSSGS 1831
                               ..::|.:..|:.:.||.:::.|:|.:.      |.|.:..|.:|.|
  Rat  1175 -------------------GPEMYEIHTSSKEERNNWMRRIQQAVE------SCPEEEGGRTSES 1214

  Fly  1832 VSGHSSQGMGSMGYPGNSQTLERPKQQITI-----VHGSHTLGKPKKKSGSQRHSAGNIDYDNLS 1891
            ..................:.|....|||..     :|....||:                   ||
  Rat  1215 DEERRKADARVAKIQQCQEILSNQDQQICTYLEEKLHIYAELGE-------------------LS 1260

  Fly  1892 GSQEADDLPPSVGVVHYASGHTHGQQMQPAGFRGRSKTVGDVTEITCSSPEPHQQQQQQQQQQQQ 1956
            |.::. .|.|     |.......|:..|.|.....:....:..::...:        .:.....|
  Rat  1261 GFEDV-HLEP-----HLLIKPDPGEPPQAASLLAAALREAESLQVAVKA--------SKLADVSQ 1311

  Fly  1957 LMQQGHAHAHPHPHPHPREPPPPPIR-------------QPHLHHHSSDIERIDPGTKSEGEE-- 2006
            ..::|...|........::.|..|..             .|.|....:|:...|.|.|.|...
  Rat  1312 PSEEGPGGAVLADTLSAQDAPASPTAFTEGTEGRGCWDVDPRLQGVVTDLAVSDAGEKVEYRSFS 1376

  Fly  2007 -DSQQGTIRPKATLGRTPNHLTLSTTSTLSVGSTGSQARLIQSSH---------------PPASY 2055
             .||...|:....|.|    |..|..:.|:          ||.||               |..|:
  Rat  1377 GSSQSEIIQAIQNLTR----LLYSLQAALT----------IQDSHIEIHKLVLQQQESLAPSHSF 1427

  Fly  2056 QPVLMKDLGKPSPDESELESESEVHAAQVAASFNLSLGSSGSLFASDARD-----NQTQSRTQSQ 2115
            :...::|..|....|...|..:.:|..|..             |..:.|.     :|.|...::|
  Rat  1428 RGGPLQDQEKSRYLEKHREELANIHKLQYQ-------------FQQEQRRWHRTCDQQQREQEAQ 1479

  Fly  2116 IHSLHPSPRQSPRQSP---RQSPKQDIEVIEIFESEAERLAASQQ-VAVVHQHPSDQARESGH-K 2175
            ...|....|:...|..   |...:.|.::.| ::...|||...|: |....|...||....|| |
  Rat  1480 ESWLQARERECQSQEELLLRHRSELDHQLQE-YQQNLERLREGQRMVERERQRMRDQQGLLGHCK 1543

  Fly  2176 QRRDRDRNPTETDATARFMDKHLSDLEQENLTDGTCDIVAYMESLREKAFDPQDLAEDTGDHQQN 2240
            ..|.|......:..:     |.:::|   |..:..|         .||:|    ...|...|...
  Rat  1544 HSRQRSLPAVFSPGS-----KEVTEL---NRAESLC---------HEKSF----FLNDAFTHMSL 1587

  Fly  2241 DSSLSPEPQ----------------TIVENTQQTVVVDIESPSRPATESDSTGNTTGPSERSGDE 2289
            ::|..|.|.                ....::|..|.:|:..||...:|..:||  .||...:..|
  Rat  1588 NTSNKPNPSGAPWDAHPPGGSHLDLARTSDSQIEVKMDVSQPSDVNSELWTTG--LGPQRPARQE 1650

  Fly  2290 TEEEATTSSLASHIVVIDKKVDKI--FKAKSPERPASTKVKGGRRQIYIS 2337
            :.:::.            |.|..:  |:.:||: |..:..:|.:.|..|:
  Rat  1651 SIKDSC------------KNVADLNSFQTESPD-PQDSNQRGPQPQTLIT 1687

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937	27/148 (18%)
		PH	873..974	CDD:278594	21/124 (17%)
		TIAM1_RBD	1141..1219	CDD:176413	17/98 (17%)
		PDZ_signaling	1221..1308	CDD:238492	8/89 (9%)
		RhoGEF	1456..1647	CDD:238091	57/222 (26%)
		PH2_Tiam1_2	1650..1823	CDD:269957	39/181 (22%)
		PH	1693..1812	CDD:278594	26/126 (21%)
Arhgef28	XP_038958459.1	C1_p190RhoGEF	659..719	CDD:410426	11/73 (15%)
		RhoGEF	866..1055	CDD:214619	55/202 (27%)
		PH_p190RhoGEF	1100..1200	CDD:275430	31/136 (23%)
		DUF5401	<1368..>1536	CDD:375164	40/195 (21%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG3519
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	3	2.810