DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment sif and Itsn2

DIOPT Version :9

Sequence 1:	NP_001261446.1	Gene:	sif / 43892	FlyBaseID:	FBgn0085447	Length:	2734	Species:	Drosophila melanogaster
Sequence 2:	XP_038969030.1	Gene:	Itsn2 / 313934	RGDID:	1590890	Length:	1716	Species:	Rattus norvegicus

Alignment Length:	1641	Identity:	325/1641 - (19%)
Similarity:	564/1641 - (34%)	Gaps:	455/1641 - (27%)

- Green bases have known domain annotations that are detailed below.

  Fly   394 STLKRMLKPMPSTESPVTSPEMGRRRYNYYNANAA--QTLGHP------PHMHQHGMAMGMGGGG 450
            :::..::.|.|...|..||        :..|..|:  |.|..|      ||...:.:.|| |.||
  Rat   148 TSIPPLMMPAPLVPSVSTS--------SLPNGTASLIQPLSIPYSSSTLPHASSYSLMMG-GFGG 203

  Fly   451 GGGHHIMNNNTMGRASSQSSRFSGSRSSHEIGRG-----YPPRNLYLE----LERERSCIEGSPP 506
            .......:...:|.:||.||..|.|.:|.:.|..     .|.|..|.:    |::..|.......
  Rat   204 ASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPSRLKYRQKFNSLDKSMSGYLSGFQ 268

  Fly   507 SDNVMFDNQCYATTPSSSNGNSDQDQSYGQQQS--------------SGQHPQQQQGPPQRSSRH 557
            :.|.:..:....|..::....:|.|.. ||.::              :|| |.....||:.....
  Rat   269 ARNALLQSNLSQTQLATIWTLADIDGD-GQLKAEEFILAMHLTDMAKAGQ-PLPLTLPPELVPPS 331

  Fly   558 QHHHQQAPNVTPTPGS--------PTSRLLLEYEMHLRNTLAKG-MDAESYSLHTFEALLSQSME 613
            ....:|..::..|..|        |..:|.:.:|...:....:| |:.|...    :.|:.|...
  Rat   332 FRGGKQIDSINGTLPSYQKTQEEEPQKKLPVTFEDKRKANYERGNMELEKRR----QVLMEQQQR 392

  Fly   614 NLE-FAESLPGSNQRSPYPLRRPNAKSSTLPLPPHRPLSTIRDKERDRDRDGYYS-DRNE----- 671
            ..| .|:......:|....|:....|..   |...:.|...|:.||.|:.:.... :|.|
  Rat   393 EAERKAQKEKEEWERKQRELQEQEWKKQ---LELEKRLEKQRELERQREEERRKEIERRESCISF 454

  Fly   672 -LIRERE-----RERDRGYLSDHNSSFSNSRCASCIGESARAQWFRHSDGWRSGSSTIGSGSGHG 730
             |:||||     ::| :|::::.                           |:.||..
  Rat   455 PLMREREWISVYKDR-KGFMAEL---------------------------WKPGSCA-------- 483

  Fly   731 MMTQQIPGSGHKRSPWDSLPSLRQDSSLNDSGYK-------SARADSLEQRAEF----------- 777
             ..|::  ...:|..|:   .:|:...||....:       :::..||....|.
  Rat   484 -AKQEL--ERQRRLEWE---RIRRQELLNQKNREQEEIVRLNSKKKSLHLELEAVNGKHQQISGR 542

  Fly   778 -----IRQDSLRSEY-LSDRESRYGIVQ----QASIESTDSRMCYLTSSEISDDDRMSLTTAVSD 832
                 ||:.:.::|. :.|::....|::    |..::...:::.||...:...::|:......:.
  Rat   543 LQDVRIRKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQLSNT 607

  Fly   833 EDDGESVM--ASPYK--------------AKATGTAASSFNCTGAVRKAGFL--SVKKWLLRKKH 879
            .|.|.|::  .|..|              .|.|.:..|..:......|.|.:  ||.:.||
  Rat   608 PDSGISLLHKKSSEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGNMDDSVLQCLL---- 668

  Fly   880 QIELARKRGWKGYWVCLKGTTLLFYPC-DSREGRSVEAAPKHLIIVDGAIMQPIPEHPKRDYIFC 943
                       ....||....||.... :|...:.:.....|.|              |||.:
  Rat   669 -----------SLLSCLNNLFLLLKELRESYNTQQLALEQLHKI--------------KRDKL-- 706

  Fly   944 LSTAFGDAYLFQAPCQVELE-NWVNSIHSACAAAFARHRGKTGTLHLLQEEIFRLEKAIESDHKL 1007
                            .||| ..:..|........|..:.|.|..:|.:|.|.:     |.:.|.
  Rat   707 ----------------KELERKRLEQIQKKKLEDEAARKAKQGKENLWKESIRK-----EEEEKQ 750

  Fly  1008 KHMAELQQSVVTDQETRHQIQTQILQWEE-----NLERLHCEQFRLRCYMASLQSGELPNPKSLL 1067
            |.:.|.:....|.:|.|   :|:..|.|.     |...|:..:.|....| |..||:
  Rat   751 KRLQEEKSQDRTQEEER---KTEAKQSETARALVNYRALYPFEARNHDEM-SFNSGD-------- 803

  Fly  1068 THVSRPTKNTLNKLGVFTVSSFHA----FIC---------ARSPSLLNNLL---AGRGATKRRP- 1115
              :.:..:.|:.:.| :...||..    |.|         .::||....||   ....||...|
  Rat   804 --IIQVDEKTVGEPG-WLYGSFQGKFGWFPCNYVEKMLSSDKTPSPKKALLPPAVSLSATSAAPQ 865

  Fly  1116 PMLSRSNS------GSSRRSMQMNSRDEPEKTFKVAMPDNAYSTVYLRDAMSVEEFLASA-CARR 1173
            |:.|...:      ..|..::.:|:..:.:..|...:...:.|.:: ....:||...|.| |:
  Rat   866 PLCSNQPAPVTDYQNVSFSNLNVNTTWQQKSAFTRTVSPGSVSPIH-GQGQAVENLKAQALCS-- 927

  Fly  1174 NLNPMEHFVRVKKRRDMEDHNYFVPHRNDLI------ENY----LHNHE--FVEVCMKILYQVEL 1226
                    ...||    |:|..|..|  |:|      ||:    :|...  |.:..:||:...|:
  Rat   928 --------WTAKK----ENHLNFSKH--DVITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEV 978

  Fly  1227 QRTTLEQMWGFSVEAELIENA----ERQDELCCYVSRVEDKSVAMHNGIIKGDEIMVI------- 1280
            :|...|.::. :|..:....|    |....|..| |.||...:.    ..:|:|::|.
  Rat   979 KRGEPEALYA-AVNKKPTSTAYPVGEEYIALYSY-SSVEPGDLT----FTEGEELLVTQKDGEWW 1037

  Fly  1281 NGAIVSDLDMM---YLESVLQEEQSLSMMMRSSRTEPPDLVGIMRVTD----DMIDSLVCPPPPT 1338
            .|:|.....:.   |:....||....:....:|..:|.    |.:||.    ...:.|...|
  Rat  1038 TGSIGERTGIFPSNYVRPKDQENVGNASKSGASNKKPE----IAQVTSAYAASGAEQLSLAP--- 1095

  Fly  1339 DPPVMSEEMITGLIVPAPGW-------NGTSKD----------LYSPEAE-SSPATSFV-DPAAM 1384
                  .::|..|...:.||       .|..:.          |..|.|| ::||...| ...||
  Rat  1096 ------GQLILILKKNSSGWWQGELQARGKKRQKGWFPASHVKLLGPSAERTTPAFHAVCQVIAM 1154

  Fly  1385 AAQLAVGGVGGVVVGGLGVAKPTSRTSSFEIENLLKTAEQETRKSSP----------TGSVTSSV 1439
            ...:|..             :.....|..::.|::       .|..|          ||...|:.
  Rat  1155 YDYIANN-------------EDELNFSKGQLINVM-------NKDDPDWWQGEINGVTGLFPSNY 1199

  Fly  1440 --STTALTPSRQ----------LTDAEKLRK-VVMELVDTERTYVKHLNNLLEHYLEPMKRETFL 1491
              .||...||:|          :...|:.|: .:.||::||..|:..|..::|.:.:.|....||
  Rat  1200 VKMTTDSDPSQQWCADLQALDTMQPMERKRQGYIHELIETEERYMDDLQLVIEVFQKRMAESGFL 1264

  Fly  1492 SNAEINALFGNIHEIVTFQRQFLQNLE-------ESLDLEPDFNKFEHCGQFRNVLFAIGSAFLY 1549
            :.||:..:|.|..|::....:.|:.|.       |.:.:|                 .:|.....
  Rat  1265 TEAEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVE-----------------MMGDILAA 1312

  Fly  1550 YVNHFKLYSSFCASHSKAQKVLHPNEGNHA-LQEFL--AARNPKQQHSSTLESYLIKPIQRILKY 1611
            .::|.:.|..||:.......:|.......| .:|||  .|.:|:.: ...|.|:|:||:|||.:|
  Rat  1313 ELSHMQAYIRFCSCQLNGAALLQQKTDEDADFKEFLKKLASDPRCK-GMPLSSFLLKPMQRITRY 1376

  Fly  1612 PLLLQQMRNLTDTRADEHVHLCEALKGMEKVAEHINEMQR------------IHEEYGAIFDHLF 1664
            |||::.:...|.....:|..|..||:..|::...:||..|            .|.:...:.:.|.
  Rat  1377 PLLIRSILENTPQNHVDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLI 1441

  Fly  1665 RQHQKSCKQPIDLSPGDLLYYGGVEWLNISDFLGKIKKGLELHAMCFVFKSAVVFLCKERLRQK- 1728
            .....:|     |.|..|||.|.         |.|.|...|||...|.....:.:|.::.....
  Rat  1442 FNSLTNC-----LGPRKLLYSGK---------LYKTKSNKELHGFLFNDFLLLTYLVRQFAASSG 1492

  Fly  1729 -KKLMGVSSKNATNEVEIIRYQVLIPVTEVQVRASSAKDMDSHFLWELIHLRSQLQRRSEKVYVL 1792
             :||.  |||::   .:...|:..|.:.||.|:..:....|.. ::.:.|:        ::||.|
  Rat  1493 FEKLF--SSKSS---AQFKMYKTPIFLNEVLVKLPTDPSSDEP-VFHISHI--------DRVYTL 1543

  Fly  1793 SNSTADFRNAFLKTIR 1808
            .....:.|.|:::.|:
  Rat  1544 RTDNINERTAWVQKIK 1559

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937	22/128 (17%)
		PH	873..974	CDD:278594	16/102 (16%)
		TIAM1_RBD	1141..1219	CDD:176413	19/90 (21%)
		PDZ_signaling	1221..1308	CDD:238492	19/100 (19%)
		RhoGEF	1456..1647	CDD:238091	50/201 (25%)
		PH2_Tiam1_2	1650..1823	CDD:269957	36/173 (21%)
		PH	1693..1812	CDD:278594	26/118 (22%)
Itsn2	XP_038969030.1	EH	15..107	CDD:197477
		KLF3_N	106..>185	CDD:410554	10/44 (23%)
		EH	238..317	CDD:197477	11/79 (14%)
		PTZ00121	<347..797	CDD:173412	97/553 (18%)
		SH3_Intersectin2_1	780..836	CDD:212921	13/67 (19%)
		SH3_Intersectin2_2	921..972	CDD:212923	15/66 (23%)
		SH3_Intersectin2_3	1004..1055	CDD:212925	11/55 (20%)
		SH3_Intersectin2_4	1076..1134	CDD:212927	10/66 (15%)
		SH3_Intersectin2_5	1149..1202	CDD:212929	10/72 (14%)
		RhoGEF	1233..1413	CDD:214619	49/197 (25%)
		PH_13	1431..1574	CDD:406942	34/157 (22%)
		C2_Intersectin	1574..1708	CDD:176021
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	0.910